Ultrasound during mid‐gestation: Agreement with physical foetal and placental measurements and use in predicting gestational age in sheep          下载免费PDF全文

AK Jones  RE Gately  KK McFadden  ML Hoffman  SM Pillai  SA Zinn  KE Govoni  SA Reed 《Reproduction in domestic animals》2017,52(4):649-654

To determine the effects of poor maternal nutrition and litter size on foetal growth during mid‐gestation, pregnant ewes (n = 82) were fed 100%, 60% or 140% of NRC TDN beginning at day 30.2 ± 0.2 of gestation. Transabdominal ultrasound was performed weekly between day 46.0 ± 0.4 and 86.0 ± 0.7 to monitor foetal heart width (HW), umbilical diameter (UMB), rib width (RW) and placentome outer (OD) and inner diameter (ID). Data were analysed with repeated‐measures using the mixed procedure for effects of maternal diet, litter size and gestation, and equations predictive of gestational age were generated using the regression procedure. To determine the agreement of ultrasound measurement and actual size, ewes (n = 20–21) were euthanized at day 45 or 90 to obtain corresponding postmortem measurements for Bland–Altman analysis. The HW, UMB and placentome OD and ID increased with gestation (p < .0001) but were unaffected by maternal diet or litter size (p ≥ .12). Ultrasound underestimated postmortem measurements of HW (14.8%), UMB (7.3%), placentome OD (4.5%) and ID (37.3%) at day 90 of gestation. Ultrasound underestimated RW at day 45 (7.7%) but overestimated RW (23.8%) at day 90, indicating inconsistent bias when reporting RW by ultrasound. Combining the HW, UMB, RW and placentome OD generated the strongest equation predictive of gestational age (R² = .91). These findings indicate that during mid‐gestation, maternal diet or litter size did not affect HW, UMB or placentome diameters and these factors can be used to estimate gestational age.  相似文献   

Endometrial biopsy in Bos indicus beef heifers          下载免费PDF全文

O Ramirez‐Garzon  N Satake  RE Lyons  J Hill  MK Holland  M McGowan 《Reproduction in domestic animals》2017,52(3):526-528

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Nephroblastoma in two dogs     

A D J WATSON  T L W ROTHWELL  J D MOORE  J M GIBBS  M F RE 《Australian veterinary journal》1987,64(3):94-96

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G02Identification of functional TRPV1 vanilloid receptor in MCF-7 cells     

R. BARBERO  P. BADINO  B. CUNIBERTI  A. MIOLO  R. ODORE  V. DI MARZO  & G. RE 《Journal of veterinary pharmacology and therapeutics》2006,29(S1):203-204

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G03Aliamides modulate skin mast cell degranulation in dogs and cats     

A. MIOLO  G. RE  R. BARBERO  M. GIORGI  R. LEOTTA  S. AUXILIA  C. NOLI  & F. ABRAMO 《Journal of veterinary pharmacology and therapeutics》2006,29(S1):204-205

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Progesterone and Caspase-3 Activation in Equine Cyclic Corpora Lutea     

G Ferreira-Dias  L Mateus  AS Costa  S Solá  RM Ramalho  RE Castro  CMP Rodrigues 《Reproduction in domestic animals》2007,42(4):380-386

Soon after ovulation, the newly formed corpus luteum (CL) starts secreting progesterone (P(4)), necessary for implantation. The CL, an ovarian transient endocrine organ, undergoes growth and regression throughout its life span. The objective of this study was to evaluate if caspase-3 mediates cell death in the equine cyclic luteal structures and relate it to luteal endocrine function. Blood and luteal tissue were collected during the breeding season after slaughter from 38 randomly assigned cycling mares. Luteal tissues were classified as corpora haemorrhagica (CH; n = 7); mid luteal phase corpora lutea (Mid-CL; n = 17); late or regressing corpora lutea (Late-CL; n = 9) and corpora albicans (CA; n = 5). Plasma P(4) concentration, determined by radioimmunoassay, showed a significant increase from CH to Mid-CL (p < 0.001), followed by a decrease to Late-CL (p < 0.001) and CA (p < 0.001). Caspase-3 processing and poly (ADP) ribose polymerase (PARP) degradation were assessed by western blotting. Active caspase-3 was twofold increased in Mid-CL, Late-CL and CA as compared with CH (p < 0.05). Immunocytochemistry also showed a significant increase in caspase-3 expression in large luteal cells in all structures when compared with CH (p < 0.05). Consistently, the endogenous caspase-3 substrate, PARP, was markedly degraded from CH to CA (p < 0.05). In fact, the ratio of full-length to degraded PARP showed a significant decrease from CH to Mid-CL, Late-CL and CA (p < 0.05). Finally, the decrease in P(4) from Mid- to Late-CL coincided with no further increases in apoptosis. In conclusion, these results suggest that the effector caspase-3 of apoptosis, might play an important role during luteal tissue involution in the mare, even though its relationship with P(4) remains to be elucidated.  相似文献   

抓低改促高产求发展——双峰乡实施毛竹丰产经营的调查     

宋军华  钟日生 《江西林业科技》2001,(Z1)

双峰乡是中国“毛竹之乡”——宜丰县的毛竹重点产区.在对毛竹林的经营中,双峰乡通过全面规划、分类改造,改革机制、多种管理方式经营,建设样板、典型带动推广,以及基地建设与竹材深加工开发相结合等一系列措施,使这个“双料”的毛竹之乡真正发挥了资源优势,实现了毛竹丰产高效,促进了地方经济发展.调查总结了其成功的竹林经营管理经验,还介绍了向全县推广的措施方法.  相似文献   

Magnetic microstructure of magnetotactic bacteria by electron holography     

RE Dunin-Borkowski  MR McCartney  RB Frankel  DA Bazylinski  M Posfai  PR Buseck 《Science (New York, N.Y.)》1998,282(5395):1868-1870

Off-axis electron holography in the transmission electron microscope was used to correlate the physical and magnetic microstructure of magnetite nanocrystals in magnetotactic bacteria. The magnetite crystals were all single magnetic domains, and the magnetization directions of small superparamagnetic crystals were constrained by magnetic interactions with larger crystals in the chains. Shape anisotropy was found to dominate magnetocrystalline anisotropy in elongated crystals. A coercive field between 300 and 450 oersted was determined for one chain.  相似文献   

Effect of breed of cattle on transmission rate and innate resistance to infection with Babesia bovis and B bigemina transmitted by Boophilus microplus     

RE BOCK  TG KINGSTON  AJ de  VOS 《Australian veterinary journal》1999,77(7):461-464

OBJECTIVE: To assess the effect of breed of cattle on the transmission rates of and innate resistance to Babesia bovis and B bigemina parasites transmitted by Boophilus microplus ticks. DESIGN: Groups of 56 purebred B indicus and 52 B indicus cross B taurus (50%, F1 generation) steers were placed in a paddock seeded with and also naturally infested with B microplus which were the progeny of females ticks fed on B taurus cattle specifically infected with a virulent isolate of B bovis. The cattle were placed in the infested paddock 50 days after seeding had started. PROCEDURE: Cattle were inspected from horseback daily for 50 days. Clinically ill cattle were brought to yards and assessed by monitoring fever, depression of packed-cell volume, parasitaemia and severity of clinical signs. Any animals that met preset criteria were treated for babesiosis. Blood samples were collected from all cattle on day 28, 35 and 42 after exposure and antibodies to Babesia spp and packed cell volume measured. RESULTS: All steers, except for one crossbred, seroconverted to B bovis and B bigemina by day 35 and 75% of the crossbred steers showed a maximum depression in packed cell volume of more than 15% due to infection with Babesia spp compared with only 36% of the B indicus group. Ten of the 52 crossbreds and 1 of the 56 B indicus steers showed severe clinical signs. Two of the crossbreds required treatment of which one died 2 weeks after initial treatment. CONCLUSIONS: Pure-bred B indicus cattle have a high degree of resistance to babesiosis, but crossbred cattle are sufficiently susceptible to warrant the use of preventive measures such as vaccination. Transmission rates of B bovis and B bigemina to B indicus and crossbred cattle previously unexposed to B microplus were the same.  相似文献   

水分胁迫下4种冷季型草坪草抗旱性比较研究   总被引：1，自引：0，他引：1  

初晓辉  岳信龙  任健  陈功  单贵莲 《草原与草坪》2012,32(4):15-19

采用盆栽育苗法,研究了4种冷季型草坪草在不同水分胁迫下的抗旱性,结果表明:1)随着水分胁迫程度的加深,4种冷季型草坪草的外观质量和叶片相对含水量显著下降,细胞质膜透性、MDA含量和Pro含量上升。2)模糊数学综合评价结果表明,4种冷季型草坪草种抗旱性能由强到弱的顺序为:高羊茅>多年生黑麦草>草地早熟禾>匍匐翦股颖。  相似文献