A comparison of the interferon gamma assay with the absorbed ELISA for the diagnosis of Johne''s disease in cattle   总被引：3，自引：0，他引：3  

H. BILLMAN-JACOBE  M. CARRIGAN†  F. COCKRAM†  LA CORNER  IJ GILL‡  JF HILL§  T. JESSEP¶  AR MILNER#  PR WOOD 《Australian veterinary journal》1992,69(2):25-28

Two interferon gamma (IFN-gamma) assays, the IFN-gamma enzyme immunoassay (EIA) and the IFN-gamma bioassay and an absorbed ELISA were used to screen 6 cattle herds for Johne's disease. Each herd had a history of Johne's disease but the majority of infected animals did not show clinical signs. The disease status of the cattle, which were removed from the herds, was confirmed by bacteriological culture of faeces or histopathological examination and culture of tissues collected at necropsy. The sensitivities of the IFN-gamma assays and the absorbed ELISA were determined using test results from infected animals. The sensitivity of the IFN-gamma EIA in detecting subclinical (71.8 to 93.3%) and clinical animals (100%) was not significantly different. However, the IFN-gamma bioassay and the absorbed ELISA were more sensitive in detecting cattle with advanced infections (80%) than those that were subclinically affected (16.7 to 33.3%).  相似文献   

Ascending placentitis in the mare: A review     

Cummins C  Carrington S  Fitzpatrick E  Duggan V 《Irish veterinary journal》2008,61(5):307-313

Ascending placentitis is a condition that occurs late in pregnancy when bacteria enter the sterile uterus from the lower reproductive tract. It leads to abortion or the birth of premature and weakened foals. Early detection and treatment of this condition is vital for ensuring the production of a viable foal.Mares with ascending placentitis often present in late term pregnancy with signs of premature udder development and premature lactation. There may be a vulvar discharge. Early detection of placental problems is possible using trans-abdominal or trans-rectal ultrasonography. Hormones such as progesterone and relaxin may be measured as indicators of foetal stress and placental failure. Postpartum foetal membranes may be thickened and contain a fibronecrotic exudate. The region most affected is the cervical star. Definitive diagnosis of ascending placentitis is by histopathological examination of the chorioallantoic membrane.Ideal treatment strategies are aimed at curing the infection and prolonging the pregnancy to as close to term as possible and consist of anti-microbials, anti-inflammatories and hormonal support.Swabs are taken from affected mares to determine antibiotic sensitivity and to aid in treatment of foals born from these mares which are at risk of becoming septic. If detected early enough, the chances of producing a viable foal are greatly increased.  相似文献   

Rams with poor feed efficiency are highly responsive to an exogenous adrenocorticotropin hormone (ACTH) challenge     

Knott SA  Cummins LJ  Dunshea FR  Leury BJ 《Domestic animal endocrinology》2008,34(3):261-268

An animal's response to a stressor is to increase metabolic rate, and thus energy consumption through the activation of the hypothalamic-pituitary-adrenal axis. Changes to energy use by an animal are likely to influence the efficiency with which it is utilised. In this study, we tested the hypothesis that less efficient sheep are more responsive to exogenous administration of adrenocorticotropin hormone. This was done by firstly determining the appropriate dose (0.4, 1.6 or 6.4microg/kg LW) and peak serum cortisol response time (45min) to exogenous administration of adrenocorticotropin hormone in a pilot study (n=3 sheep). Following this, adrenocorticotropin hormone (2.0microg/kg LW) stimulated cortisol levels were measured in a larger group of sheep (n=50) of known feed efficiency (feed conversion ratio and residual feed intake values). Less efficient sheep (more positive residual feed intake values) were found to have a greater (P<0.001) increase in cortisol concentration in comparison to more efficient animals. Those sheep which had higher levels of cortisol also had a greater proportion (P<0.001) of fat tissue. These data clearly demonstrated that efficiency of energy use, when measured as residual feed intake, is significantly related to an animal's stress response. These findings have important implications for understanding the physiological mechanisms underpinning efficiency of energy use, and may be useful in successfully identifying animals which are superior in terms of feed efficiency.  相似文献   

Development of a pathogen transport model for Irish catchments using SWAT     

R. Coffey  E. Cummins  N. Bhreathnach  V.O. Flaherty  M. Cormican 《Agricultural Water Management》2010,97(1):101-111

SWAT (Soil and Water Assessment Tool) represents a dynamic catchment modelling application that can be applied to any river basin and used to quantify the impact of land management practices on water quality over a continuous period. The objective of this study is to apply the Soil and Water Assessment Tool (SWAT) to model pathogen transport, simulate management practices affecting water quality and predict pathogen loads in Irish catchments. Based on input data regarding agricultural practice, demographics and hydrological parameters for the river Fergus catchment, SWAT was run to predict concentrations of Escherichia coli. Hydrometric validation results display a very good linear relationship between observed and predicted data (Coefficient of determination R² = 0.83, Nash-Sutcliffe efficiency E = 0.78) and indicate satisfactory simulation of hydrologic processes within the catchment. To date, pathogen predictions have proved variable between observed and simulated figures. Based on recommended values for the quantification of catchment modelling accuracy, predictions for E. coli can be described as acceptable and satisfactory with R² = 0.68 and E = 0.59. Extensive monitoring is required for such simulations and the current study represents partial validation. Results suggest that although the capabilities exist to simulate pathogen transport in catchments, the capacity to accurately account for all factors that can contribute to water degradation is uncertain. The sensitivity analysis identified the bacteria partition coefficient (BACTKDDB) as the most important input parameter. In addition it reveals areas where further research is required, particularly in assessing the initial concentration of E. coli in human/animal waste. The developed model provides a tool capable of protecting water sources and human health from waterborne pathogens.  相似文献   

大豆遗传资源保护的法律法规及其可专利性     

丁海  骆素芳 《分子植物育种》2004,2(4):465-472

大豆起源于中国,中国是世界上大豆遗传资源最为丰富的国家,也曾经是世界上最主要的大豆生产国和出口国。半个多世纪以来,科学技术的进步带动了一些美洲国家大豆产业的迅猛发展,中国的大豆生产量由世界首位降到美国、巴西之后,从大豆出口国变为大豆净进口国,大豆成为中国农产品贸易中最令国际社会关心的贸易作物。随着农业生物技术的快速发展,知识产权保护的法律法规的国际化,尤其是我国加入WTO以后,大豆研究、开发、生产领域遇到了来自比其他作物前所未有的挑战。作为我国最重要的植物蛋白、食用油及动物饲料来源的大豆作物,有效地利用有关的法律法规保护我国大豆种质资源及新品种已经迫在眉睫。本文着重介绍了与大豆遗传资源、新品种保护相关的我国法律法规,进行了大豆保护的可专利性分析,并对大豆研发过程中的知识产权保护问题作了初步探讨,以促进对大豆新品种的申请保护,提高我国应对来自国外竞争的抗衡能力。  相似文献   

Effect of Cyclin‐dependent Kinase (CDK) Inhibition on Expression,Localization and Activity of Maturation Promoting Factor (MPF) and Mitogen Activated Protein Kinase (MAPK) in Bovine Oocytes     

MD Quetglas  PR Adona  THC De Bem  PRL Pires  CLV Leal 《Reproduction in domestic animals》2010,45(6):1074-1081

  相似文献   

A sandwich enzyme immunoassay for bovine interferon-γ and its use for the detection of tuberculosis in cattle   总被引：14，自引：0，他引：14  

JS ROTHEL  SL JONES†  LA CORNER  JC COX†  PR WOOD 《Australian veterinary journal》1990,67(4):134-137

An in vitro cellular assay for bovine tuberculosis has recently been developed. This assay detects gamma-interferon released in response to specific antigen in a whole blood culture system. The bio-assay previously described for the detection of bovine gamma-interferon (IFN-gamma) has now been replaced with a sandwich enzyme immunoassay (EIA) which utilises two monoclonal antibodies to bovine IFN-gamma. The EIA detects less than 25pg/ml of recombinant bovine IFN-gamma and is specific for biologically active bovine IFN-gamma; and does not detect bovine alpha or beta interferon. IFN-gamma from sheep, goat and buffalo, but not from pig, deer or man, are also recognised by the EIA. The bovine IFN-gamma EIA when used in conjunction with the whole blood culture system has resulted in a simple, rapid and sensitive in vitro assay for specific cell mediated immune responsiveness to M. bovis infection in cattle.  相似文献   

Developmental changes in the regulation of plasma growth hormone concentrations in Holstein calves     

J L Sartin  R J Kemppainen  K A Cummins  D N Marple  J C Williams 《American journal of veterinary research》1991,52(9):1397-1400

A study was initiated to determine whether development of a functional ruminant digestive system was associated with alterations in plasma growth hormone (GH) concentration. Holstein bull calves were fed milk or milk with grain until studied at the age of 1 month (n = 12). Calves placed on pasture with some grain supplementation were studied at the age of 3 months (n = 6) to determine plasma GH concentration in an animal with fully developed ruminant metabolism. Blood samples were taken at 10-minute intervals for 5 hours, followed by administration of bovine GH-releasing factor (0.075 micrograms/kg of body weight) and subsequent blood sample collection for 1 hour. On the following day, a blood sample was collected via jugular cannula, clonidine (10 micrograms/kg) was administered, and blood samples were subsequently obtained. Data indicated that milk-fed calves had higher mean plasma GH concentration than did either milk/grain-fed or older calves. The difference in mean plasma GH concentration was related to higher secretory pulse amplitude. Pituitary responses to bovine GH-releasing factor did not differ among the 3 groups, but response to clonidine were greater in milk-fed calves than in calves of the other groups. These data indicate that the change from a nonruminant to a ruminant-type gastrointestinal tract, perhaps attributable to subsequent changes in metabolism, may induce changes in hypothalmic function to decrease GH concentration.  相似文献   

Neutralizing antibody responses to bluetongue and epizootic hemorrhagic disease virus serotypes in beef cattle     

R W Fulton  L J Burge  J M Cummins 《American journal of veterinary research》1989,50(5):651-654

Blood samples were obtained from sentinel beef cattle at monthly intervals, and the sera were tested for antibodies, using a bluetongue virus (BTV) immunodiffusion test (IDT) and virus-neutralization test (VNT), for 5 BTV serotypes (2, 10, 11, 13, and 17) and 2 epizootic hemorrhagic disease virus (EHDV) serotypes (1 and 2). The cattle tested were transported from Tennessee to Texas in 1984 and 1985. All cattle were seronegative by the BTV IDT at the initial bleeding in Texas in 1984 and 1985. In 1984, 16 of 40 (40%) cattle seroconverted as assessed by results of the BTV IDT. In the 16 seropositive cattle in 1984, neutralizing antibodies were detected to BTV serotypes 10 (n = 7), 11 (n = 3), and 17 (n = 11), and EHDV serotypes 1 (n = 1) and 2 (n = 7). In 1984, no cattle seroconverted to BTV-2 or BTV-13. In 1985, 10 of 36 (27.8%) cattle seroconverted as assessed by results of the IDT. Of the 10 seropositive cattle in 1985, neutralizing antibodies were detected to BTV serotypes 10 (n = 10), 11 (n = 10), 13 (n = 7), and 17 (n = 5), and EHDV serotypes 1 (n = 1) and 2 (n = 7). In 1985, no cattle seroconverted to BTV-2. Clinical diseases attributable to BTV or EHDV was not detected in these cattle in 1984 or 1985.  相似文献   

Efficiency of inspection procedures for the detection of tuberculous lesions in cattle   总被引：2，自引：0，他引：2  

LA CORNER  L. MELVILLE†  K. McCUBBIN‡  KJ SMALL†  BS McCORMICK§  PR WOOD  JS ROTHEL 《Australian veterinary journal》1990,67(11):389-392

The sensitivity of the abattoir inspection procedure introduced for Australian export beef in 1976 was compared to a detailed necropsy procedure for the detection of tuberculous lesions in cattle. In a sample of cattle that were reactors to the tuberculin test, abattoir inspection failed to detect an estimated 47% of cattle with lesions. The detailed necropsy examination of cattle with lesions of tuberculosis identified 21 sites of infection compared with 13 to 18 in cattle examined by routine meat inspection procedures. Of the lesions detected during detailed necropsy, 15.9% did not involve the thoracic cavity or the medial retropharyngeal lymph nodes. The failure to detect lesions during abattoir inspection has its greatest significance in an animal with a single lesion. If the 245 cattle found with single lesions during detailed necropsy had been examined by abattoir inspection using the 1976 or the 1986 procedures, 0.8 and 8.9%, respectively, of these animals would not have been detected because the diseased tissues would not have been examined. If meat inspection is to provide an effective means of monitoring the level of bovine tuberculosis during the final stages of eradication, a procedure no less sensitive than that introduced in 1976 should be used.  相似文献