Effect of testicular tissue lysate on developmental competence of porcine oocytes matured and fertilized in vitro          下载免费PDF全文

AK Singh  S Naskar  B Saikia  Y Vashi  S Gupta  S Banik  MK Tamuli  V Pande  DK Sarma  SK Dhara 《Reproduction in domestic animals》2017,52(2):183-188

The objective of the present study was to investigate the effect of testicular tissue lysate (TTL) on developmental competence of germinal vesicle (GV) stage porcine oocytes. Two types of TTL were prepared through repeated freeze–thaw in liquid nitrogen, one from whole testicular tissue (wTTL) and other from either of four different sections of testes, namely just beneath the tunica albuginea (TA), from the transitional area between the seminiferous cord/tubules and the mediastinum testis (TR) and from the intermediate area (parenchymal tissue origin) and CE (cauda epididymis origin). The whole or section‐wise TTL treatments were given for 44 hr during in vitro maturation (IVM). Oocyte maturation was done in either of the two media, namely defined (high‐performance basic medium for porcine oocyte maturation, commercially available) and serum containing (TCM199). After maturation, oocytes were co‐incubated with fresh spermatozoa for 6 hr and then transferred to embryo culture media. Treatment of GV stage oocytes with wTTL (1 mg/ml) increased the cleavage and morula percentage rate (69.23 ± 6.23 and 48.15 ± 6.77, respectively) than that of their control (58.33 ± 8.08 and 32.54 ± 5.53, respectively) in defined media, and in serum‐containing media, cleavage and morula percentage rate were almost equal in both treatment (54.56 ± 7.79 and 34.70 ± 6.78, respectively) and control (59.52 ± 8.21 and 38.52 ± 6.54, respectively). However, effect of wTTL was not significant. In case of section‐wise TTL supplements, TR section significantly (p < .01) improved cleavage and morula rate (58.43 ± 7.98 and 36.14 ± 6.89, respectively) followed by TA. In conclusion, present study indicates that IVM, in vitro fertilization and in vitro culture of embryo are improved in the presence of TTL, particularly its TR section. Further study is expected to reveal the principal components of TTL which may prove useful for IVM.  相似文献   

Euthanasia; not always Eu     

DK BLACKMORE 《Australian veterinary journal》1993,70(11):409-413

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Signalling Events and Associated Pathways Related to the Mammalian Sperm Capacitation          下载免费PDF全文

DK Gangwar  SK Atreja 《Reproduction in domestic animals》2015,50(5):705-711

Capacitation is a biological phenomenon occurring prior to fertilization and is a multiple event process. Many physiological and biochemical changes takes place during the process; these changes are related to lipid composition of membrane, intracellular modulation of ion concentration, protein phosphorylation, sperm movement and membrane permeability. These events occur when the sperm is exposed to the new environment of ion concentration in the female reproductive tract. Ions such as bicarbonate and calcium facilitate capacitation by activating adenylyl cyclase, thus initiating protein kinase A (PKA) signalling cascade. Extracellular‐regulated kinase pathway is activated by ligand binding to the membrane receptors and intracellular activation by reactive oxygen species (ROS). Activation of these pathways leads to the phosphorylation of different proteins, which is associated with events such as capacitation, hyperactivation and acrosome reaction that are essential for successful fertilization. Extensive studies were carried out on protein phosphorylation in relation to capacitation, but its role still remains ambiguous.  相似文献   

Bovine Circadian Locomotor Output Cycles Kaput (CLOCK) and Clusterin (CLU) mRNA Quantitation in Ejaculated Crossbred Bull Spermatozoa          下载免费PDF全文

S Kumar  R Deb  U Singh  I Ganguly  DK Mandal  S Tyagi  M Kumar  G Sengar  S Sharma  R Singh  R Singh 《Reproduction in domestic animals》2015,50(3):505-509

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Management of equine skull fractures using fixation with polydioxanone sutures     

KL Schaaf  NJ Kannegieter  DK Lovell 《Australian veterinary journal》2008,86(12):481-485

Ten horses presented with severe distortion of the facial contour, crepitus on palpation and mild to moderate epistaxis. Individual horses also showed ocular damage, ptosis, severe dyspnoea and movement of the facial bones concurrent with respiration. The fracture fragments were exposed using a large curvilinear incision and elevated using a retractor, periosteal elevator, chisel or Steinmann pin. The fracture fragments were unstable following reduction and fixation was necessary. Stabilisation was achieved with polydioxanone sutures placed through holes drilled in opposing sides of the fracture lines. Polydioxanone sutures provided good stability and had better handling properties than wire. There was good apposition of fracture edges and minimal complications. Use of polydioxanone sutures can also avoid the expense and complexity of plate fixation in selected cases, and should be considered as an alternative to fixation with stainless steel wire in any facial fracture that adjoins stable bone.  相似文献   

Impacts of pollutant inputs on forest canopy condition in the UK: possible role of P limitations   总被引：1，自引：0，他引：1  

Harrison  AF; Carreira  J; Poskitt  JM; Robertson  SMC; Smith  R; Hall  J; Hornung  M; Lindley  DK 《Forestry》1999,72(4):367-378

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Effect of grassland degradation on aggregate‐associated soil organic carbon of alpine grassland ecosystems in the Qinghai‐Tibetan Plateau     

Shikui Dong  Jing Zhang  Yuanyuan Li  Shiliang Liu  Qugnaming Dong  Huakun Zhou  Jane Yeomans  Yv Li  Shuai Li  Xiaoxia Gao 《European Journal of Soil Science》2020,71(1):69-79

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The impact of introduced signal crayfish Pacifastacus leniusculus on stream invertebrate communities     

Lynne Crawford  William E. Yeomans  Colin E. Adams 《水产资源保护:海洋与淡水生态系统》2006,16(6):611-621

• 1. The effect of a newly introduced population of signal crayfish (Pacifastacus leniusculus) on a riverine invertebrate community in the River Clyde, Scotland, is examined. Five hypotheses related to crayfish impact on the invertebrate community are tested by comparing sites containing signal crayfish with similar sites in the same river which have not yet been colonized.
• 2. The hypothesis that crayfish significantly reduce the total numbers of invertebrates in the community was supported by this study, with invertebrate density in areas containing signal crayfish around 60% of that with no observed crayfish.
• 3. The hypothesis that crayfish presence affects some groups more than others was also supported. Plecoptera, Chironomidae, Diptera and Hirudinea, in particular, showed evidence of reduced species numbers in areas containing crayfish, and Plecoptera, Hirudinea, Tricladida and Hydracarina showed evidence of reduced densities. Community diversity and richness was also lower at sites containing crayfish.
• 4. Neither the hypothesis that overall invertebrate biomass would be lower at sites with crayfish present nor the hypothesis that individual invertebrate size would be lower at sites with crayfish was supported.
• 5. Overall the study indicates that introduced signal crayfish can significantly and fundamentally modify fluvial macroinvertebrate community structure.

Copyright © 2006 John Wiley & Sons, Ltd.  相似文献   

Limited inhibition of nitrous oxide reduction in soil in the presence of acetylene   总被引：2，自引：0，他引：2  

Jane C. Yeomans  E.G. Beauchamp 《Soil biology & biochemistry》1978,10(6):517-519

Anaerobic flasks with two different soils contained microorganisms which effectively reduced NO₃⁻ to N₂ in the absence of C₂H₂ and in the presence or absence of CO₂. In the presence of C₂H₂, the microorganisms reduced NO₃⁻ to N₂O and the further reduction of N₂O to N₂ was temporarily inhibited. This was shown for two partial pressures of C₂H₂ (0.1 kPa and 1.0 kPa). However. after a maximum of 168 h, microorganisms were able to reduce N₂O to N₂ in the presence of C₂H₂. This was shown in the presence of CO₂ for both partial pressures of C₂H₂ and in the absence of CO₂ for 1.0 kPa C₂H₂. The absence of CO₂ delayed the complete reduction of N₂O. Microorganisms which had reduced N₂O in the presence of C₂H₂ retained this ability for at least 3 days after the original atmosphere containing C₂H₂ had been removed.  相似文献   

牛DES基因的SNPs检测     

狄江  Cynthia DK Bottem  BozenaKruk 《西北农业学报》2004,13(3):1-4

设计了6套引物对利木赞(Limousin)和娟姗(Jersey)两个牛品种杂交一代的3头公牛进行DES基因的PCR扩增和测序,除引物4外均获得扩增产物,并在引物1扩增产物的上、下游及引物2扩增产物的上游序列的非编码区中各发现一个SNP,分别为SNP1(A/G)、SNP2(C/T)和SNP3(T/G)。同时测得公牛1、2、3的SNP1、SNP2、SNP3的基因型分别为A/A、A/G、A/G,C/C、C/C、C/T,T/G、G/G、G/G。  相似文献