Salmonella serotypes isolated from the aquatic environment (Wabash River, Indiana, 1973-1976).     

E V Morse  M A Duncan  E P Myhrom 《American journal of veterinary research》1978,39(4):717-719

During 1973-1976, 833 isolations of salmonellae were made from the aquatic biosphere of the Wabash River, Lafayette, Ind. Of the isolants, 643 were definitively serotyped. Salmonella typhimurium represented 34.4% of the cultures. Salmonella eimsbuettel previously had not been recognized in Indiana. A total of 35 serotypes and 1 untypable group C1 was obtained from the aquatic samples, ie, river water, river bottom sediment, fish, and mussels. Most of the isolants were presumed to be of human origin, because many samplings were made in close proximity to sewage treatment plant outfalls. During the survey period, epidemic salmonellosis had not been reported for the Lafayette-West Lafayette metropolitan area.  相似文献   

Quantification of imidacloprid toxicity to avocado thrips, Scirtothrips perseae Nakahara (Thysanoptera: Thripidae), using a combined bioassay and ELISA approach     

Byrne FJ  Toscano NC  Urena AA  Morse JG 《Pest management science》2005,61(8):754-758

A competitive ELISA (enzyme-linked immunosorbent assay) technique was evaluated for quantifying titres of imidacloprid in homogenates of leaf discs sampled from avocado plants treated with systemic applications of imidacloprid 240 g litre(-1) SC (Admire). Matrix effects were evident with undiluted leaf tissue homogenates, but these were effectively eliminated by dilution of homogenates in water. In a field trial conducted in a commercial nursery, there was an excellent correlation between imidacloprid residues within leaves and avocado thrips (Scirtothrips perseae Nakahara) mortality. However, with subsequent sampling over a 2-month period, the relationship between mortality and apparent imidacloprid concentration became less robust, suggesting that the material was being degraded within the plant to non-toxic metabolites. Nevertheless, assessments of thrips mortality on leaves that had been recently treated with imidacloprid established a lower threshold of activity for imidacloprid residues of 6 ng cm(-2) leaf. Limitations on the use of ELISA to quantify the impact of systemic insecticides on pest populations are discussed.  相似文献   

Effects of pH, sodium bicarbonate, cryoprotectants and foetal bovine serum on the cryopreservation of European eel sperm.     

DL Garzón  DS Peñaranda  L Pérez  F Marco‐Jiménez  X Espert  T Müller  M Jover  JF Asturiano 《Reproduction in domestic animals》2008,43(1):99-105

The main objective of the present study was to evaluate the influence of pH and bicarbonate concentration in the activation or inhibition of European eel (Anguilla anguilla) spermatozoa and to evaluate the effect of different cryoprotectants: dimethyl sulphoxide (DMSO), acetamide, ethylene glycol, propanol, glycerol and methanol (MeOH). The effect of these factors was evaluated comparing the percentage of motile cells, the percentage of alive cells (by Hoechst staining) and the spermatozoa morphometry pre- and post-cryopreservation (by computer-assisted morphology analysis). Based on the above findings, three cryoprotectants (DMSO, MeOH and glycerol) were chosen and evaluated in two media (P1 and P1 modified) with different concentrations of NaHCO(3) and in the presence or absence of foetal bovine serum (FBS). The effect of these factors was evaluated comparing the percentage of alive and motile cells post-cryopreservation. DMSO was the cryoprotectant showing better results in relation to the percentage of spermatic alive cells post-freezing and caused a smaller modification of the head spermatozoa morphology. The combination of P1-modified medium with DMSO and containing FBS increased slightly but significantly the percentage of motile spermatozoa post-cryopreservation.  相似文献   

Perceptions of Whooping Cranes among waterfowl hunters in Alabama: using specialization,awareness, knowledge,and attitudes to understand conservation behavior     

Sarah K. Lessard  Wayde C. Morse  Christopher A. Lepczyk  Erin Seekamp 《Human Dimensions of Wildlife》2018,23(3):227-241

A central component of successful wildlife conservation is the understanding of public perceptions toward a species. We administered an online survey to Alabama waterfowl hunters (n = 284) to examine differences in awareness and knowledge of and attitudes toward Whooping Cranes. We investigated if these variables and/or birding specialization influenced likeliness to participate in conservation behaviors. Specialization level was positively related to awareness, knowledge, and conservation attitudes. Respondents with greater knowledge and favorable attitudes toward cranes were more likely to donate to a hypothetical crane conservation fund, and expert birdwatchers were more likely to belong to a birding organization than novice or intermediate birdwatchers. Our research supplements existing birding specialization literature by incorporating cognitive variables specific to Whooping Cranes. Findings suggested that enhancing knowledge regarding Whooping Crane conservation may be an advantageous strategy for promoting conservation and positive behaviors toward the species.  相似文献   

Detection of Mycoplasma gallisepticum by direct immunofluorescence using a species-specific monoclonal antibody     

J W Morse  J T Boothby  R Yamamoto 《Avian diseases》1986,30(1):204-206

A monoclonal antibody against Mycoplasma gallisepticum (MG) (strain S6) was prepared in mice and identified as isotype IgG1 by standard procedures. Although it did react at high titers (1:100,000) in the enzyme-linked immunosorbent assay (the original method for its identification), it failed to react in the agglutination, hemagglutination-inhibition, and growth-inhibition tests. When conjugated to fluorescein isothiocyanate, the monoclonal antibody reacted with the homologous and eight "atypical" strains of MG but not with M. meleagridis or M. synoviae in the direct fluorescent-antibody test. This reagent may be useful for detecting field infections involving atypical strains of MG.  相似文献   

FIREFLIES FLASHING IN UNISON     

Morse ES 《Science (New York, N.Y.)》1918,48(1230):92-93

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Toxicity of systemic neonicotinoid insecticides to avocado thrips in nursery avocado trees   总被引：1，自引：0，他引：1  

Byrne FJ  Toscano NC  Urena AA  Morse JG 《Pest management science》2007,63(9):860-866

The efficacies of four systemic neonicotinoid insecticides applied to potted avocado trees at manufacturer-recommended rates were assessed against the avocado thrips, Scirtothrips perseae Nakahara. At the time of treatment, fully expanded first-flush young leaves were tagged for identification, and a proportion of these leaves was used in bioassays with second-instar thrips. At 7 weeks post-treatment, a second flush of leaves had fully expanded on the trees, and these leaves were included in additional bioassays comparing avocado thrips mortality on both first- and second-flush leaves. In bioassays with first-flush leaves, imidacloprid (273 mg AI pot(-1)) was the most effective insecticide, providing at least 70% mortality of thrips for 14 weeks. Thiamethoxam (137 mg AI pot(-1)), clothianidin (109 mg AI pot(-1)) and dinotefuran (241 mg AI pot(-1)) provided good control in bioassays that were conducted within 4 weeks of treatment, but thereafter their efficacies were inconsistent. In bioassays with second-flush leaves, imidacloprid provided at least 70% mortality up to 9 weeks after the insecticide application. Thereafter, mortality declined to 30% or lower. Bioassays with second-flush leaves collected from trees treated with thiamethoxam, clothianidin and dinotefuran resulted in unacceptably low thrips mortality. Monitoring of imidacloprid and thiamethoxam residues by ELISA showed that the greater persistence of imidacloprid in both first and second leaf flushes was due to a steadier uptake of this material. Although thiamethoxam residues rose quickly within the first leaf flush, levels had already begun to dissipate by the time the second leaf flush had started to develop.  相似文献   

THE EFFICIENCY OF HALOGENS IN INDUCING METAMORPHOSIS IN FROG LARVAe     

Morse M 《Science (New York, N.Y.)》1914,40(1039):793-794

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FIREFLIES FLASHING IN UNISON     

Morse ES 《Science (New York, N.Y.)》1916,44(1133):387-388

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OBSERVATIONS UPON A YELLOWS DISEASE OF THE FALL DANDELION     

Morse WJ 《Science (New York, N.Y.)》1908,28(715):348-349

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