Genetics of stem rust resistance in the durum wheat accession ‘Reichenbachii’     

Efrem Bechere  E. L. Smith  F. J. Gough  O. W. Merkle 《Euphytica》1991,53(2):103-106

Summary Inheritance of seedling reactions to cultures of races TLM and MBCT of Puccinia graminis f.sp. tritici was studied in crosses of the resistant durum Reichenbachii with susceptible Marruecos 9623 and DZ04-118. The Chi-square test for goodness of fit was used to analyze segregating populations. Two independent recessive genes appeared to control resistance of Reichenbachii to both TLM and MBCT. Depending upon the cross, the two genes interacted cumulatively to condition immunity or higher levels of resistance than either parent conditioned singly. There was no evidence of maternal influence on the inheritance of resistance. Resistance genes in Reichenbachii were recessive; therefore, breeders should consider the use of relatively large F₂ populations to effectively transfer these genes to cultivars with good agronomic characteristics.  相似文献   

真空袋装储藏对荞麦品质影响的试验研究     

杨晓清  杜文亮  赵丽芹  陈忠军  代丽君 《内蒙古农业大学学报(自然科学版)》2003,24(1):56-59

本文针对荞麦在收获后到食用时品质下降过快的问题，在理论分析的基础上，采用真空袋装方法对荞麦米就储藏期间的品质保持问题进行了研究。试验结果表明：真空袋装能有效地抑制荞麦米储藏期间游离脂肪酸浓度的增长。真空度保持在80Kpa以上时，其游离脂肪酸浓度下降为同期常规储藏的42％左右：在荞麦米的色泽保持方面，避光条件下，真空袋装能很好地保持荞麦米原有色泽，但在光照条件下，它的护绿效果却很不明显，只略好于常规储藏。  相似文献   

Impact of lower atmospheric carbon dioxide on tropical mountain ecosystems   总被引：1，自引：0，他引：1  

FA Street-Perrott  Y Huang  RA Perrott  G Eglinton  P Barker  LB Khelifa  DD Harkness  DO Olago 《Science (New York, N.Y.)》1997,278(5342):1422-1426

Carbon-isotope values of bulk organic matter from high-altitude lakes on Mount Kenya and Mount Elgon, East Africa, were 10 to 14 per mil higher during glacial times than they are today. Compound-specific isotope analyses of leaf waxes and algal biomarkers show that organisms possessing CO2-concentrating mechanisms, including C4 grasses and freshwater algae, were primarily responsible for this large increase. Carbon limitation due to lower ambient CO2 partial pressures had a significant impact on the distribution of forest on the tropical mountains, in addition to climate. Hence, tree line elevation should not be used to infer palaeotemperatures.  相似文献   

Evaluation of a Simulation Training Program for Uncomplicated Fishhook Removal     

Scott McMaster  DO David Ledrick  Julies Stausmire 《The Forestry Chronicle》2013,24(3):292

  相似文献   

Time‐coordinated prevalence of extracellular HGF,FGF2 and TGF‐β3 in crush‐injured skeletal muscle     

Mai‐Khoi Q. DO  Takahiro SUZUKI  Borjigin GERELT  Yusuke SATO  Wataru MIZUNOYA  Mako NAKAMURA  Yoshihide IKEUCHI  Judy E. ANDERSON  Ryuichi TATSUMI 《Animal Science Journal》2012,83(10):712-717

Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon‐guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early‐differentiated satellite cells up‐regulate a secreted neural chemorepellent Sema3A upon in vivo muscle‐crush injury. The study also revealed that Sema3A expression is up‐regulated in primary satellite‐cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) and is prevented by transforming growth factor (TGF)‐β2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time‐course of extracellular HGF, FGF2 and TGF‐β3 concentrations after crush‐injury of Gastrocnemius muscle in the rat lower hind‐limb, using a combination of a non‐homogenization/non‐spin extraction of extracellular wound fluids and enhanced chemiluminescence–Western blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 2–8 days post‐crush, whereas active TGF‐β3 increases after 12 days, providing a better understanding of the time‐coordinated levels of HGF, FGF2 and TGF‐β3 that drive regulation of Sema3A expression during regenerative intramuscular moto‐neuritogenesis.  相似文献   

越南水稻黄矮病毒多克隆血清抗体的制备          下载免费PDF全文

何越强  陈氏懦花  陈阮河  杜新勇  阮德辉  韦善富  覃武  吕荣华 《南方农业学报》2019,50(7):1472-1482

[目的]制备用于检测水稻黄矮病毒(RYSV)的多克隆抗体,为水稻和黑尾叶蝉上的病毒诊断提供技术支持.[方法]利用两种不同来源的RYSV抗原免疫家兔,一种是从受感染的水稻病叶组织纯化获得RYSV病毒粒子;另一种是从越南RYSV分离株克隆出完整N蛋白编码基因,然后将其连接至pET-28a载体上,在大肠杆菌Rosetta(DE3)菌株中进行诱导表达获得N蛋白抗原.其中,N蛋白抗原又以两种形式(洗脱纯化N蛋白和N蛋白条带聚丙烯酰胺切片匀浆)对家兔进行免疫.最后,采用PTA-ELISA分析评估家兔抗体血清(多克隆抗体)对水稻叶片和黑尾叶蝉RYSV的特异性和敏感性.[结果]分离自越南RYSV分离株的N基因由1542个核苷酸组成,与来自我国和日本RYS分离株N基因序列进行比对,其核苷酸序列同源性分别为98.1%和97.9%,对应的推导氨基酸序列同源性为83.4%和99.4%.以RYSV病毒粒子、洗脱纯化N蛋白和N蛋白条带聚丙烯酰胺切片匀浆3种抗原免疫家兔获得的多克隆抗体均能有效检测出水稻植株中的RYSV,其中,感病植株的OD405分别为1.449、2.337和1.649,健康植株的OD405分别为0.375、0.294和0.283.PTA-ELISA检测结果表明,获得的多克隆抗体能从单个带病毒黑尾叶蝉中检测出RYSV,且该结论在RT-PCR检测中得到进一步验证.[结论]制备获得的多克隆抗体对RYSV具有较高特异性和敏感性,可用于水稻和黑尾叶蝉上的病毒诊断,同时表明以含有病毒植物蛋白的聚丙烯酰胺切片直接注射免疫模型动物制备多克隆抗体具有可行性.  相似文献   

A 'new' strain of infectious bronchitis virus infecting domestic fowl in Great Britain.   总被引：5，自引：0，他引：5  

R E Gough  C J Randall  M Dagless  D J Alexander  W J Cox  D Pearson 《The Veterinary record》1992,130(22):493-494

  相似文献   

Massive liver hemorrhage in Ontario broiler chickens     

A W Gough  L J Weber 《Avian diseases》1978,22(1):205-210

A massive liver hemorrhage (MLH) recognized in Ontario broiler chickens had a characteristic clinical course and pathological lesions. Affected flocks had a higher death rate than normal, with no obvious clinical signs. Deaths from MLH began at two weeks of age; the mortality rate returned to a normal level by four weeks. The main necropsy finding was massive multiple liver hemorrhage with consequent profuse hemoperitoneum.  相似文献   

Studies with an atypical avian rotavirus from pheasants     

R E Gough  G W Wood  D Spackman 《The Veterinary record》1986,118(22):611-612

  相似文献   

OBSERVATIONS ON THE BULB AND POTATO APHID RHOPALOSIPHONINUS LATYSIPHON (DAVIDSON) IN EAST ANGLIA     

T. J. Legowski  H. C. Gough 《Plant pathology》1953,2(4):126-130

  相似文献