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The freeze preservation of coccidia   总被引:1,自引:0,他引:1  
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REASONS FOR PERFORMING STUDY: A safe, affordable and effective treatment for endotoxaemia in horses is needed in order to reduce the incidence of this potentially fatal condition. OBJECTIVE: To evaluate the effect of polymyxin B (PMB) on signs of experimentally-induced endotoxaemia. HYPOTHESIS: PMB ameliorates the adverse effects of endotoxaemia without causing nephrotoxicity. METHODS: Four groups of 6 healthy mature horses each received 20 ng endotoxin/kg bwt i.v. over 30 mins. Additionally, each group received one of the following i.v.; 5000 u PMB/kg bwt 30 mins before endotoxin infusion; 5000 u PMB/kg bwt 30 mins after endotoxin infusion; 1000 u PMB/kg bwt 30 mins prior to endotoxin infusion; or saline. Clinical response data and samples were collected to determine neutrophil count, serum tumour necrosis factor (TNF) activity, plasma thromboxane B2 concentration and urine gamma glutamyltranspeptidase (GGT) to creatinine ratio. RESULTS: Treatment with PMB before or after administration of endotoxin significantly reduced fever, tachycardia and serum TNF, compared to horses receiving saline. The differences in response to endotoxin were greatest between horses that received saline vs. those that received 5000 u PMB/kg bwt prior to endotoxin. Urine GGT:creatinine did not change significantly. CONCLUSIONS AND POTENTIAL RELEVANCE: This study indicates that PMB may be a safe and effective treatment of endotoxaemia, even when administered after onset. Although nephrotoxicity was not demonstrated with this model, caution should be exercised when using PMB in azotaemic patients.  相似文献   
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Six wild-caught, captive canyon tree frogs (Hyla arenicolor) purchased as a group and housed at the North Carolina Zoological Park were diagnosed with intradermal Hannemania mites, the first described infestation of this frog species by this mite. Typical gross lesions were orange-colored skin vesicles approximately 1 mm in diameter, predominantly located on the ventrum and ventral hind limbs. The larval mites had ovoid bodies approximately 44 microm in length and 240 microm in width. Three of the frogs died, and three became free of mites and pathologic changes after approximately 28 wk of treatment with transcutaneous ivermectin and manual mite removal. The frogs remained free of mites at a 1-yr recheck.  相似文献   
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Perennial grasses provide forage and environmental benefits in the agricultural systems of southern Australia, but persistent cultivars are lacking for the lower rainfall inland margins (<600 mm average annual rainfall). Potential new cultivars of cocksfoot (Dactylis glomerata L.), tall fescue (Festuca arundinacea Schreb. = syn. Lolium arundinaceum (Schreb.) Darbysh.) and phalaris (Phalaris aquatica L.) have been developed in response to this need. These and older cultivars were evaluated for persistence at five sites in south‐eastern Australia from 2009 to 2013. A wide range in rainfall was experienced, but survival was principally reduced by a severe spring–summer–autumn drought in the final year in which all species began to decline in persistence once the spring 2012 to autumn 2013 cumulative climatic moisture deficit became higher than about 700 mm and declined very severely at deficits over 1000 mm. The highly summer‐dormant cocksfoot ssp. hispanica control cultivar Kasbah showed outstanding survival of this event in the cropping zone of southern New South Wales, but not at the drier of two sites with acidic soils in central Victorian where all cocksfoot cultivars showed similar survival. Potential new summer‐active tall fescue cultivars survived well at the higher rainfall Victorian site and under high rainfall in northern NSW. All tall fescue cultivars experienced high mortality during a period of high moisture stress in the spring of the establishment year in southern NSW, but the Mediterranean cultivar Grasslands Flecha MaxP gradually recovered. Differences among phalaris cultivars were not large with new cultivars of North African origin being marginally more persistent at the drier site in southern NSW. Comparisons with results from the Mediterranean Basin are discussed. Although there was no clear evidence of differences in persistence between the new and old cultivars, some of the new cultivars were considered to show potential for commercial release.  相似文献   
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A retrospective review of case records of ultrasonography and necropsy outcomes of 62 koalas was used to investigate the accuracy of ultrasonography in assessing koala urogenital tract structural disease at the Port Macquarie Koala Hospital. The results showed high concordance, supporting ultrasonography as an effective tool for evaluating structural disease of the koala urogenital tract, most commonly seen with chlamydiosis. The study also illustrates the advances benefiting animal welfare that can be made by wildlife carer groups through using a scientific, evidence‐based approach.  相似文献   
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External oscillating electric fields (1166 volts per centimeter, 5 hertz) enhanced the incorporation of [3H] thymidine into the DNA of chondrocytes isolated from the proliferative layer of embryonic (16 days) chick epiphysis. Verapamil or tetrodotoxin at 10(-6)M concentrations completely blocked the electric field effect. Tetracaine reduced the incorporation of [3H] thymidine in both control and electrically stimulated cells. The findings support the hypothesis that Na and Ca2 fluxes generated by the electrical perturbation trigger DNA synthesis in these cells.  相似文献   
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