旋毛虫成虫丝氨酸蛋白酶抑制剂的原核表达及免疫原性研究     

李达  万悦  马静  孔令杰  路佳琦  武文浩  路义鑫 《中国兽医杂志》2015,(3):17-19,23

以旋毛虫成虫的总RNA为模板,应用RT-PCR扩增获得旋毛虫成虫丝氨酸蛋白酶抑制剂(TsAdSPI)基因。经克隆鉴定正确后,将TsAdSPI基因连接到pET-28a表达载体,转化至大肠杆菌BL21(DE3)中进行IPTG诱导表达,并将纯化后的目的蛋白免疫新西兰大白兔,制备多克隆抗体。应用Western-blot及ELISA方法对重组蛋白的免疫原性和抗体效价进行分析。SDS-PAGE结果显示,目的蛋白分子质量为44 k Da,以包涵体形式表达。经Western-blot和ELISA方法分析,制备的多克隆抗体效价可高达1∶51200,表明该蛋白具有较好免疫原性。表明TsAdSPI蛋白可作为旋毛虫感染早期血清学诊断方法的候选抗原,为新型的抗旋毛虫及相关寄生虫药物研发打下结实基础。  相似文献   

浅议冰蓄冷低温送风系统在商务建筑中的应用     

马达 《林业科技情报》2012,(3):40-42

冰蓄冷低温送风系统因其显著的节能效果而受到越来越多的关注,本文从其系统结构特点、初投资与运行费用等方面简述其经济与社会效益,并通过对某商务建筑空调系统的介绍,有针对性地探讨了该空调形式在商务建筑中应用的可行性。  相似文献   

利用辐射不育技术防治柑桔大实蝇的示范试验   总被引：5，自引：0，他引：5  

王华嵩  胡建国 《中国生物防治》1995,11(4):156-159

在贵州省惠水县１０万株温州蜜结构，约１１８ｈｍ＾２的７个桔园内，于１９９３和１９９４年分别释放辐射不育柑桔大实蝇约６０万头和１００万头，使柑桔大实蝇的为害率由释放前３年的５．１９４％下降到１９９３年的０．１３４％和１９９４年的０．０９８％。  相似文献   

Inheritance of resistance to a Bacillus thuringiensis toxin in a field population of diamondback moth (Plutella xylostella)     

Amparo C. Martínez-Ramírez  Baltasar Escriche  M. Dolores Real  Francisco J. Silva  Juan Ferr 《Pest management science》1995,43(2):115-120

Inheritance of resistance to the Bacillus thuringiensis Berl. CryIA(b) crystal protein was studied in Plutella xylostella L. (diamondback moth). A field population 50-fold more resistant to CryIA(b) than a control susceptible strain was used. Dose-mortality curves of the resistant population, the susceptible strain and the F₁ from the two reciprocal crosses were compared. Resistance transmission to the F₁ was dependent on the sex of the resistant progenitor. Sex ratio of the survivors to high doses of CryIA(b) in the F₁ of the two reciprocal crosses did not corroborate the preliminary hypothesis of resistance being due to a recessive sex-linked allele. Since, in a previous work, the loss of CryIA(b) binding capacity of resistant insects had been demonstrated, binding to midgut tissue sections from F₁ individuals was also analysed. The presence of binding in all of the F₁ preparations showed that, at least, a recessive autosomal allele was responsible for the loss of binding capacity in the resistant population.  相似文献   

Relationships between kidney mass and body size in some Anseriformes     

Kalisińska E  Dańczak A  Pierko M  Wysocki D 《Anatomia, histologia, embryologia》1999,28(1):55-60

Relationships between kidney mass (KM) and body mass (BM), body length (BL), and sternum length (SL) were studied in adults of both sexes of 4 Mergini anseriforms: Clangula hyemalis (n = 74), Melanitta fusca (n = 29), M. nigra (n = 15), and Somateria mollissima (n = 8). The following indices were established for the four species and for the Mergini tribe: KM/BM (as per cent body mass), KM/BL, and KM/SL. Additionally, allometric equations describing the relationships studied were developed for the tribe using mean kidney weights and body parameters of males and females of the species examined. The KM/BM indices for several anseriform tribes (i.e. Anserini, Anatini, Aythyini and Mergini), differing in food and feeding modes, were determined, based on the literature data and those obtained in this study. In addition, an allometric equation describing the kidney weight-body weight relationship in the Anseriformes order was developed as log KM = 0.797 log BM-1.346 (n = 22). The relative kidney size in the sea duck species studied showed significant intra- and interspecific differences. In addition, clear between-tribes differences in KM/BM were revealed. The highest value (1.57%) of the index is typical of the Mergini, grouping diving carnivorous sea ducks, while the lowest index (0.65%) is typical of the Anserini, a tribe which groups non-diving herbivorous birds.  相似文献   

Detection of genomic variability in different populations of the cattle tick Boophilus microplus in southern Brazil     

Passos DT  Ferreira CA  da Silva SS  Richter MF  Ozaki LS 《Veterinary parasitology》1999,87(1):83-92

DNA from seven isolates of the cattle tick Boophilus microplus was analyzed by restriction fragment length polymorphism (RFLP). Three different cDNA clones, named P-9, P-25 and CP-12, isolated from a B. microplus cDNA library, were used as DNA probes. DNA sequences of P-9 have high similarity to ribosomal genes, whereas P-25 does not show significant homology with known sequences within databases. CP-12 is a cDNA clone encoding a cysteine endopeptidase gene. A limited degree of polymorphism was detected with P-9 and P-25, while CP-12 showed a different pattern of bands for each tick isolate. These findings suggest the existence of a complex genotypic diversity of the tick B. microplus population in endemic regions.  相似文献   

Experimental infection of calves with a gI, gE, US9 negative bovine herpesvirus type 5     

Hübner SO  Oliveira AP  Franco AC  Esteves PA  Silva AD  Spilki FR  Rijsewijk FA  Roehe PM 《Comparative immunology, microbiology and infectious diseases》2005,28(3):187-196

In this work, a role for the genes encoding glycoproteins I (gI) and E (gE) and the US9 protein of bovine herpesvirus type 5 (BHV-5) in neuropathogenicity and reactivation of latent infections was examined. Calves infected intranasally with a gI/gE/US9 deleted recombinant shed up to 10(2.85) TCID50/ml infectious virus in nasal secretions. Calves infected with the wild type BHV-5 parental virus shed up to 10(5) TCID50/ml virus. No signs of disease were observed in calves infected with the recombinant virus, whereas those infected with wild type virus displayed respiratory and neurological signs. The recombinant was only able to reach the basal portions of the central nervous system. In contrast, wild type virus was found widespread within the brain. Reactivation with dexamethasone 60 days post-infection resulted in reactivation of wild type virus, whereas the recombinant virus could not be reactivated. These studies demonstrate that genes gI, gE and US9 of BHV-5 are important for its neuropathogenicity and its ability to reactive from latency.  相似文献   

Feline leishmaniasis due to Leishmania (Leishmania) amazonensis in Mato Grosso do Sul State, Brazil     

de Souza AI  Barros EM  Ishikawa E  Ilha IM  Marin GR  Nunes VL 《Veterinary parasitology》2005,128(1-2):41-45

A case of leishmaniasis in a domestic cat (Felis domesticus) is described. The animal showed a single, nodular lesion on the nose and many nodules of different size on the ears and digital regions of all the paws. Diagnosis was made by microscopic detection of amastigotes in Giemsa-stained smears from the lesions. By monoclonal antibodies the aetiological agent was identified as Leishmania (Leishmania) amazonensis, one of the seven species implicated in human leishmaniasis in Brazil. The clinical signs in feline leishmaniasis are unspecific and similar to those observed in other diseases such as cryptococcosis and in sporotrichosis, commonly found in cats. Leishmaniasis should therefore, be added to the differential diagnosis by feline veterinary practitioners and adequate investigations should carried out for dermal leishmaniasis in the area where the feline infection is detected.  相似文献   

Sero-epidemiological survey for toxoplasmosis in wild New World monkeys (Cebus spp.; Alouatta caraya) at the Paraná river basin, Paraná State, Brazil     

Garcia JL  Svoboda WK  Chryssafidis AL  de Souza Malanski L  Shiozawa MM  de Moraes Aguiar L  Teixeira GM  Ludwig G  da Silva LR  Hilst C  Navarro IT 《Veterinary parasitology》2005,133(4):307-311

In this study, we captured 60 wild New World monkeys (Cebus spp.; Alouatta caraya) at the Paraná river basin, Paraná State, Brazil, and modified agglutination test (MAT) was performed to evaluate anti-Toxoplasma gondii antibodies. Prevalence was 30.2% (13/43) in Cebus spp. (capuchin monkeys) and 17.6% (3/17) for A. caraya (black and golden howler monkeys). MAT showed antibody titers of 16 (15/16) and 64 (1/16). Herein, we have observed an odds ratio (OR)=4.67 (1.060.05). The present work is the first report on serum occurrence of anti-T. gondii antibodies in wild capuchin monkeys and in wild black and golden howler monkeys.  相似文献   

Immunogenicity in dogs of three recombinant antigens (TSA, LeIF and LmSTI1) potential vaccine candidates for canine visceral leishmaniasis     

Fujiwara RT  Vale AM  França da Silva JC  da Costa RT  Quetz Jda S  Martins Filho OA  Reis AB  Corrêa Oliveira R  Machado-Coelho GL  Bueno LL  Bethony JM  Frank G  Nascimento E  Genaro O  Mayrink W  Reed S  Campos-Neto A 《Veterinary research》2005,36(5-6):827-838

Control of canine visceral leishmaniasis (VL) remains a difficult and serious problem mostly because there is no reliable and effective vaccine available to prevent this disease. A mixture of three recombinant leishmanial antigens (TSA, LeIF and LmSTI1) encoded by three genes highly conserved in the Leishmania genus have been shown to induce excellent protection against infection in both murine and simian models of cutaneous leishmaniasis. A human clinical trial with these antigens is currently underway. Because of the high degree of conservation, these antigens might be useful vaccine candidates for VL as well. In the present study, using the dog model of the visceral disease, we evaluated the immunogenicity of these three antigens formulated with two different adjuvants, MPL-SE and AdjuPrime. The results were compared with a whole parasite vaccine formulated with BCG as the adjuvant. In order to investigate if sensitization with the recombinant antigens would result in recognition of the corresponding native parasite antigens upon infection, the animals were exposed for four weeks after the termination of the immunization protocol with the recombinant antigens to a low number of L. chagasi promastigotes, an etiological agent of VL. Immune response was evaluated by quantitative ELISA in the animal sera before and after exposure to the viable parasites. Both antigen specific IgG1 and IgG2 antibody levels were measured. Immunization of dogs with the recombinant antigens formulated in either MPL-SE or AdjuPrime resulted in high antibody levels particularly to LmSTI1. In addition, this immunization although to low levels, resulted in the development of antibody response to the whole parasite lysate. Importantly, experimental exposure with low numbers of culture forms of L. chagasi promastigotes caused a clear boost in the immune response to both the recombinant antigens and the corresponding native molecules. The boost response was predominantly of the IgG2 isotype in animals primed with the recombinant antigens plus MPL-SE. In contrast, animals primed with the recombinant antigens formulated in AdjuPrime as well as animals vaccinated with crude antigen preparation responded with mixed IgG1/IgG2 isotypes. These results point to the possible use of this antigen cocktail formulated with the adjuvant MPL-SE in efficacy field trials against canine VL.  相似文献