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81.
82.
Effect of testicular tissue lysate on developmental competence of porcine oocytes matured and fertilized in vitro
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AK Singh S Naskar B Saikia Y Vashi S Gupta S Banik MK Tamuli V Pande DK Sarma SK Dhara 《Reproduction in domestic animals》2017,52(2):183-188
The objective of the present study was to investigate the effect of testicular tissue lysate (TTL) on developmental competence of germinal vesicle (GV) stage porcine oocytes. Two types of TTL were prepared through repeated freeze–thaw in liquid nitrogen, one from whole testicular tissue (wTTL) and other from either of four different sections of testes, namely just beneath the tunica albuginea (TA), from the transitional area between the seminiferous cord/tubules and the mediastinum testis (TR) and from the intermediate area (parenchymal tissue origin) and CE (cauda epididymis origin). The whole or section‐wise TTL treatments were given for 44 hr during in vitro maturation (IVM). Oocyte maturation was done in either of the two media, namely defined (high‐performance basic medium for porcine oocyte maturation, commercially available) and serum containing (TCM199). After maturation, oocytes were co‐incubated with fresh spermatozoa for 6 hr and then transferred to embryo culture media. Treatment of GV stage oocytes with wTTL (1 mg/ml) increased the cleavage and morula percentage rate (69.23 ± 6.23 and 48.15 ± 6.77, respectively) than that of their control (58.33 ± 8.08 and 32.54 ± 5.53, respectively) in defined media, and in serum‐containing media, cleavage and morula percentage rate were almost equal in both treatment (54.56 ± 7.79 and 34.70 ± 6.78, respectively) and control (59.52 ± 8.21 and 38.52 ± 6.54, respectively). However, effect of wTTL was not significant. In case of section‐wise TTL supplements, TR section significantly (p < .01) improved cleavage and morula rate (58.43 ± 7.98 and 36.14 ± 6.89, respectively) followed by TA. In conclusion, present study indicates that IVM, in vitro fertilization and in vitro culture of embryo are improved in the presence of TTL, particularly its TR section. Further study is expected to reveal the principal components of TTL which may prove useful for IVM. 相似文献
83.
SUMMARY The epidemiological and clinical features of big liver and spleen disease (BLS) in flocks on two broiler breeder farms were investigated by serology and gross pathology. The most common necropsy findings on farm 1 were splenomegaly and hepatomegaly, with kidney enlargement in some birds. In one flock (farm 1), a decline in egg production began at 40 weeks of age and lasted for 9 weeks. Seroconversion to BLS antigen was first detected at 45 weeks (3.1% of birds) and increased to 72% at 50 weeks, which coincided with clinical recovery in the flock. Antigen was detected before antibody at 44 weeks and persisted at low incidence (<15%). Farm egg production statistics and serology indicated that the disease affected all flocks on the farm. In three of eight flocks, seroconversion was detected in birds before peak production. The birds in the remaining sheds did not seroconvert or become sick until after peak production. On the second farm, sampling began within a flock already experiencing BLS. Clinical signs and pathology were similar to those seen in flocks on farm 1. However, the lesions that were seen in the pancreas in 15% of birds have not been reported previously. BLS antibody was detected in 78%, and circulating antigen in 14%, of sick birds. 相似文献
84.
A strain of Tetranychus urticae (Koch; Acari: Tetranychidae), collected from hops (Humulus humuli L; Cannabaceae) in England with a short history of tebufenpyrad use, exhibited resistance to four METI (mitochondrial electron transport inhibitor)-acaricides; tebufenpyrad, pyridaben, fenazaquin and fenpyroximate. Resistance factors for these compounds in a microimmersion assay were 46, 346, 168 and 77 respectively, and corresponded to those exhibited by a Japanese METI-acaricide-resistant reference strain. Levels of resistance remained stable without further selection, and selection with tebufenpyrad did not increase them. The UK strain was also resistant (c 6-fold) to bifenthrin. Crosses of homozygous, diploid females with hemizygous, haploid males showed that, in the UK strain, METI-acaricide resistance was paternally and maternally inherited, and was an incompletely dominant trait. Another tebufenpyrad-resistant strain from the UK, originating from a chrysanthemum nursery (Chrysanthemum foeniculaceum Giseke; Asteraceae) was collected eight months later at a site c 210 km distant from the first. These are the first published incidences of METI-acaricide resistance in Europe and implications for the future use of these compounds are discussed. 相似文献
85.
The naphthoquinones and the METI group of compounds act on sites associated with mitochondrial respiration, but METI-resistant strains of two-spotted spider mite from Japan and the UK exhibited no cross-resistance to the naphthoquinones. The potential for developing commercial naphthoquinones therefore remains high. 相似文献
86.
Evolutionary genetics. Insecticide resistance on the move 总被引:1,自引:0,他引:1
87.
Capacitation is a biological phenomenon occurring prior to fertilization and is a multiple event process. Many physiological and biochemical changes takes place during the process; these changes are related to lipid composition of membrane, intracellular modulation of ion concentration, protein phosphorylation, sperm movement and membrane permeability. These events occur when the sperm is exposed to the new environment of ion concentration in the female reproductive tract. Ions such as bicarbonate and calcium facilitate capacitation by activating adenylyl cyclase, thus initiating protein kinase A (PKA) signalling cascade. Extracellular‐regulated kinase pathway is activated by ligand binding to the membrane receptors and intracellular activation by reactive oxygen species (ROS). Activation of these pathways leads to the phosphorylation of different proteins, which is associated with events such as capacitation, hyperactivation and acrosome reaction that are essential for successful fertilization. Extensive studies were carried out on protein phosphorylation in relation to capacitation, but its role still remains ambiguous. 相似文献
88.
89.
Woolley R Blundell R Else R Corcoran B Devine C French A 《The Journal of small animal practice》2007,48(8):455-457
A 10-year-old male cairn terrier cross was presented with a history of myxomatous mitral valve disease diagnosed six months previously and with a four-week history of intermittent collapse. On 24 hour electrocardiograph (Holter) analysis, periods of no discernable electrical cardiac activity, which coincided with three collapsing episodes, were identified. Unfortunately, on re-presentation for removal of the Holter monitor, the dog collapsed and died. A post-mortem examination was conducted, and histology of the right and left atrium showed evidence of myocarditis. This is the first reported case, to our knowledge, of collapse because of electrical asystole in a dog with atrial myocarditis. 相似文献
90.