Myofibroblastic fibrosarcoma with multifocal osseous metaplasia at the site of equine influenza vaccination     

NJ Kannegieter  KL Schaaf  DK Lovell  CD Simon  BM Stone 《Australian veterinary journal》2010,88(4):132-136

We describe a fibrosarcoma in a 12-year-old Quarterhorse × Arabian gelding as a sequela to equine influenza vaccination. Shortly after the second vaccination, swelling at the site was noticed by the owner and it continued to increase in size over the following 6 months. Biopsy of the mass indicated a fibrosarcoma had developed at the vaccination site. It was approximately 20 cm in diameter and elevated well above the level of the skin. There was no clinical evidence of metastases to the lungs or local lymph nodes. Surgical resection of the mass was performed and the wound healed by first and second intention. Histopathological examination and immunohistochemical staining confirmed a myofibroblastic fibrosarcoma with multifocal osseous metaplasia. To the authors' knowledge, this is the first equine case of a vaccine-associated fibrosarcoma.  相似文献   

Erratum     

ML Lepherd  PJ Canfield  GB Hunt  PC Thomson  KL Bosward 《Australian veterinary journal》2011,89(7):238-238

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Restriction Enzyme Analysis of Tissue Culture-adapted Velogenic Newcastle Disease Virus     

Mohan CM  Dey S  Kumanan K 《Veterinary research communications》2006,30(4):455-466

A velogenic Newcastle disease virus isolate typed to belong to group C1 by monoclonal antibody typing was adapted 50 times in chicken embryo fibroblast cell culture and 60 times in Vero cells. At every 10th passage the virus was characterized on the basis of mean death time, intracerebral pathogenicity indices and viral titration studies. A gradual reduction in the virulence of the virus was noted as the passage number increased. RT-PCR of a 254 bp region of the fusion gene encompassing the fusion protein cleavage site was carried out for the virulent as well as cell culture-adapted viruses at every 10th passage level. The amplicons were subsequently digested with three restriction enzymes, viz. AluI, HaeIII and PstI. It was found out that there was difference in banding patterns between the virulent and adapted viruses, indicating nucleotide substitutions in the virulent virus when it was sequentially passaged onto cell culture systems.  相似文献   

Prevalence of infestation with gastrointestinal nematodes in Pony Club horses in Victoria     

KL Flanagan  JM Morton  RM Sandeman 《Australian veterinary journal》2013,91(6):241-245

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Isolation of anacardic acid from natural cashew nut shell liquid (CNSL) using supercritical carbon dioxide     

Philip JY  Da Cruz Francisco J  Dey ES  Buchweishaija J  Mkayula LL  Ye L 《Journal of agricultural and food chemistry》2008,56(20):9350-9354

Solvent extracted cashew nut shell liquid (CNSL), conventionally known as natural CNSL, is a mixture of several alkenyl phenols. One of these alkenyl phenols is anacardic acid, which is present at the highest concentration. In view of anticipated industrial applications of anacardic acid, the objective of this work was to isolate anacardic acid from natural CNSL by supercritical carbon dioxide (scCO 2). In this study, the solubility data for natural CNSL in scCO 2 under a range of operating conditions of pressure (100, 200, and 300 bar), temperature (40 and 50 degrees C), and CO 2 flow rate (5, 10, and 15 g min (-1)) were established. The best scCO 2 working conditions were found to be 50 degrees C and 300 bar at a flow rate of 5 g min (-1) CO 2. Using 3 g of sample (CNSL/solid adsorbent = 1/2) under these scCO 2 conditions, it was possible to quantitatively isolate high purity anacardic acid from crude natural CNSL (82% of total anacardic acid) within 150 min. The anacardic acid isolated by scCO 2 was analyzed by different spectroscopic techniques (UV-vis, FT-IR, and (1)H NMR) and HPLC analysis, indicating that the anacardic acid isolated by scCO 2 has better quality than that obtained through a conventional method involving several chemical conversion steps.  相似文献   

Effect of fire severity on physical and biochemical soil properties in Zagros oak (<Emphasis Type="Italic">Quercus brantii</Emphasis> Lindl.) forests in Iran     

M. Heydari  A. Rostamy  F. Najafi  D. C. Dey 《林业研究》2017,28(1):95-104

Fire affects the physical and chemical properties and soil biological activity of natural ecosystems. This study was conducted in the Miyan Tang region, Ilam Province in western Iran. The study site was 110 hectares, where we sampled soils in areas that were classified by fire severity: low (LS), high (HS) and medium severity (MS), and unburned (UB), which served as the control. In each severity class, 25 transect points were randomly selected for measurement. Around each transect plot center, 3 soil samples were selected randomly and soils collected from the 0 to 20 cm depth were combined into a composite sample that was used in laboratory analysis to represent conditions at that point. Plots in the UB and LS fire classes had similar soil conditions and had higher values of factors such as saturated moisture, organic carbon, carbon dioxide, and silt and clay content. In contrast, plots in the HS and MS fire severity classes were clustered in the positive direction along the first axis that represented gradients in soil acidity, electrical conductivity, cation exchange capacity, accessible phosphorus, accessible potassium, bulk density, and sand. Soil attributes were similar in areas of HS and MS fire severity classes, whereas soil conditions in the LS class and UB controls were most similar. Fire in the LS areas either did not significantly alter the physical–chemical soil properties and microbial basal respiration, or soils were able to recover quickly after being burned.  相似文献   

Quantifying flooding effects on hardwood seedling survival and growth for bottomland restoration     

John M. Kabrick  Daniel C. Dey  J. W. Van Sambeek  Mark V. Coggeshall  Douglass F. Jacobs 《New Forests》2012,43(5-6):695-710

Growing interest worldwide in bottomland hardwood restoration necessitates improved ecological understanding of flooding effects on forest tree seedlings using methodology that accurately reflects field conditions. We examined hardwood seedling survival and growth in an outdoor laboratory where the timing, depth, duration, and flow rate of flood water can be carefully controlled while simulating natural soil conditions occurring in floodplains. Flooding treatments were initiated in mid-May and included partial inundation (15–20?cm) during the growing season for 5-week flowing, 5-week standing, 3-week flowing, and control. We monitored the vigor, survival, and growth (changes in basal diameter and stem length) of six hardwood species representing a wide range in expected flood tolerance including eastern cottonwood (Populus deltoides Bartr. Ex Marsh.), pin oak (Quercus palustris Muenchh.), swamp white oak (Q. bicolor Willd.), bur oak (Q. macrocarpa Michx.), black walnut (Juglans nigra L.), and pecan [Carya illinoensis (Wangenh.) K. Koch]. All stock was 1-0 bareroot except that cuttings were used for eastern cottonwood. Five species—eastern cottonwood, bur oak, swamp white oak, pin oak, and pecan—exhibited high survival probabilities (>0.62 for cottonwood; >0.77 for the others) regardless of flood treatment. But of the survivors, only eastern cottonwood and swamp white oak maintained positive growth and healthy green foliage. Despite high survival, bur oak and pin oak suffered stem growth losses and exhibited chlorotic foliage in flood treatments suggesting greater vulnerability to other abiotic or biotic stresses if outplanted on flood-prone sites. Pecan also suffered stem dieback in controls suggesting vulnerability to competition and browsing when outplanted despite high survival after flooding. Our quantitative data helps to confirm and/or refine previously published qualitative flood tolerance ratings for these species, and describes operation of an in situ outdoor flood experiment laboratory that may prove effective in guiding future flood tolerance research.  相似文献   

A scanning electron microscope study of Raillietascaris varani (Baylis et Daubney, 1922) (Nematoda: Ascaridioidea).     

N C De  J Dey 《Folia parasitologica》1992,39(2):145-151

The Raillietascaris varani (Baylis et Daubney, 1922), parasitic in the Indian monitor lizard, Varanus monitor (L.), was studied by scanning electron microscopy (SEM). A surface topography of the worm, especially the head and male tail, were described, illustrated and discussed with regard to the available data on this species, and also other species of ascaridoid nematodes. The present study confirms the previous observations from Sprent (1985), and also provides some additional information concerning the microtopography of the worm which might be useful in comparative studies on the Ascaridoidea.  相似文献   

First detection of bovine noroviruses and detection of bovine coronavirus in Australian dairy cattle          下载免费PDF全文

SJ Symes  JL Allen  PD Mansell  KL Woodward  KE Bailey  JR Gilkerson  GF Browning 《Australian veterinary journal》2018,96(6):203-208

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The effects of PPR on the reproductive health of Black Bengal goats and the possible role played by oxidative stress     

Pankaj Kumar  Amitava Dey  Abhay Kumar  Pradeep Kumar Ray  Poolangulam Chinnakkan Chandran  Rashmi Rekha Kumari  Manish Kumar 《Tropical animal health and production》2018,50(7):1441-1447

Outbreaks of Peste des petits ruminants (PPR) viral disease in Black Bengal goats were investigated from the middle Indo-Gangetic Plains of India. Clinical profile of PPR-affected flocks was recorded from four different outbreak sites of the region. The PPR outbreak was diagnosed serologically using commercially available sandwich ELISA kit. Relatively, low mortality rate (mean 26.75%) for PPR outbreak was recorded due to the endemic status of the disease. To understand the role of oxidative stress in PPR virus pathogenesis, various oxidant and antioxidant parameters in goats infected with PPR were estimated and compared with the uninfected/healthy goats of the same flock. The measured high level of pro-oxidant malondialdehyde (MDA) obtained from lipid peroxidation along with lower levels of anti-oxidants viz. superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in PPR-affected Black Bengal goats suggests oxidative stress as one of the mechanism of pathogenesis of PPR virus. In addition, the correlation of oxidative stress due to PPR and the resulting reproductive disorders in the female goats were evaluated. The abortion in pregnant does observed during PPR outbreak was proportional to debility and oxidative stress manifested during PPR infection. The reproductive performance of recovered female goats in the period of 18 months of monitoring was significantly compromised in terms of kidding and twinning frequency. The mortality rate in kids born from PPR-recovered goats was significantly higher compared to those from health goats in the first 9 months post-recovery. From the present study, it may be concluded that together with the PPR virus, infection in goats and the resulting oxidative stress play a vital role for abortion and reduced post-reproductive performance in Black Bengal female goat.  相似文献