Prolactin stimulation of maternal behavior in female rats   总被引：9，自引：0，他引：9  

R S Bridges  R DiBiase  D D Loundes  P C Doherty 《Science (New York, N.Y.)》1985,227(4688):782-784

Inexperienced, hypophysectomized female rats treated with steroids were used in experiments to investigate the roles of the pituitary gland and prolactin in the expression of maternal behavior. Administration of ovine prolactin or treatment with ectopic pituitary grafts, which release prolactin into the circulation, stimulated maternal care in these females toward rat young. Steroid treatment alone, while stimulating maternal behavior in rats with intact pituitary glands, did not facilitate maternal responsiveness in hypophysectomized females. These findings indicate a stimulatory behavioral role for pituitary prolactin in the establishment of maternal care and suggest that exposure to prolactin during pregnancy helps to stimulate the immediate onset of maternal behavior at parturition.  相似文献   

Stimulation by phagocytosis of the deiodination of L-thyroxine in human leukocytes   总被引：7，自引：0，他引：7  

K A Woeber  G F Doherty  S H Ingbar 《Science (New York, N.Y.)》1972,176(38):1039-1041

Intact human leukocytes actively deiodinate L-[(131)1] thyroxine, producing mainly inorganic (131)1 and chromatographically immobile (131)1-labeled origin material. When phagocytosis is induced, the deiodination is enhanced, a suggestion that deiodination in mediated by a peroxidase-hydrogen peroxide system. l-Thyroxine can serve as a source of iodine for iodination reactions within the leukocyte.  相似文献   

Mycobacterial Ku and ligase proteins constitute a two-component NHEJ repair machine     

Della M  Palmbos PL  Tseng HM  Tonkin LM  Daley JM  Topper LM  Pitcher RS  Tomkinson AE  Wilson TE  Doherty AJ 《Science (New York, N.Y.)》2004,306(5696):683-685

In mammalian cells, repair of DNA double-strand breaks (DSBs) by nonhomologous end-joining (NHEJ) is critical for genome stability. Although the end-bridging and ligation steps of NHEJ have been reconstituted in vitro, little is known about the end-processing reactions that occur before ligation. Recently, functionally homologous end-bridging and ligation activities have been identified in prokarya. Consistent with its homology to polymerases and nucleases, we demonstrate that DNA ligase D from Mycobacterium tuberculosis (Mt-Lig) possesses a unique variety of nucleotidyl transferase activities, including gap-filling polymerase, terminal transferase, and primase, and is also a 3' to 5' exonuclease. These enzyme activities allow the Mt-Ku and Mt-Lig proteins to join incompatible DSB ends in vitro, as well as to reconstitute NHEJ in vivo in yeast. These results demonstrate that prokaryotic Ku and ligase form a bona fide NHEJ system that encodes all the recognition, processing, and ligation activities required for DSB repair.  相似文献   

Identification of a DNA nonhomologous end-joining complex in bacteria     

Weller GR  Kysela B  Roy R  Tonkin LM  Scanlan E  Della M  Devine SK  Day JP  Wilkinson A  d'Adda di Fagagna F  Devine KM  Bowater RP  Jeggo PA  Jackson SP  Doherty AJ 《Science (New York, N.Y.)》2002,297(5587):1686-1689

In eukaryotic cells, double-strand breaks (DSBs) in DNA are generally repaired by the pathway of homologous recombination or by DNA nonhomologous end joining (NHEJ). Both pathways have been highly conserved throughout eukaryotic evolution, but no equivalent NHEJ system has been identified in prokaryotes. The NHEJ pathway requires a DNA end-binding component called Ku. We have identified bacterial Ku homologs and show that these proteins retain the biochemical characteristics of the eukaryotic Ku heterodimer. Furthermore, we show that bacterial Ku specifically recruits DNA ligase to DNA ends and stimulates DNA ligation. Loss of these proteins leads to hypersensitivity to ionizing radiation in Bacillus subtilis. These data provide evidence that many bacteria possess a DNA DSB repair apparatus that shares many features with the NHEJ system of eukarya and suggest that this DNA repair pathway arose before the prokaryotic and eukaryotic lineages diverged.  相似文献   

Comparing Ecological Site Descriptions to Habitat Characteristics Influencing Greater Sage-Grouse Nest Site Occurrence and Success     

Kevin E. Doherty  Jeffrey L. Beck  David E. Naugle 《Strength and Conditioning Journal》2011,64(4):344-351

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Akabane disease in sheep   总被引：1，自引：0，他引：1  

KG HAUGHEY  WJ HARTLEY†  AJ DELLA-PORTA‡  MD MURRAY§ 《Australian veterinary journal》1988,65(5):136-140

Perinatal lamb mortality, associated with malformations of the CNS due to Akabane viral infection, occurred in 4 of 9 flocks of ewes lambing on 3 farms between 26 May and 14 November, 1976. Cases were restricted to ewes conceiving prior to the second week of March and lambing between 26 May and 19 July. As judged by seroconversion in sentinel flocks on 2 of the farms, field infection with Akabane virus occurred mainly between mid-February and mid-April. Malformations of the CNS occurred in 42.5%, 51.2%, 100% and 31.0% of the dead lambs examined in the affected flocks respectively. Prevalence in the 4 affected flocks, expressed as the proportion of ewes lambing which delivered at least one malformed foetus, was 6.1%, 8.4%, 88.9% and 5.7% respectively. Lamb mortality due to malformations of the CNS was 7.1%, 5.5%, 92.3% and 5.7% of lambs born. Age-specific prevalence was calculated for 3 of the 4 flocks and 2-year-old ewes accounted for 71.4% and 76.9% of total cases respectively in 2 flocks, whereas in one flock malformations occurred at equivalent frequencies throughout several older age groups. Birthweights of affected lambs were usually significantly lighter than those of unaffected lambs of similar sex and birth-type, and their mean duration of gestation was slightly, and significantly, prolonged. Micrencephaly (88.1% of cases) and hydrocephalus (68.7% of cases) were the outstanding pathological features of the malformations with hydranencephaly, microgyria, porencephaly and attenuation of the spinal cord occurring at much lower frequencies.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

VIRUS STRAINS ISOLATED FROM ARTHROPODS DURING AN EPIZOOTIC OF BOVINE EPHEMERAL FEVER IN QUEENSLAND   总被引：4，自引：0，他引：4  

R L Doherty  J G Carley  H A Standfast  A L Dyce  W A Snowdon 《Australian veterinary journal》1972,48(3):81-86

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ANTERIOR CRUCIATE LIGAMENT RECONSTRUCTION USING A COMPOSITE COLLAGENOUS PROSTHESIS: A BIOMECHANICAL AND HISTOLOGIC STUDY IN RABBITS     

Dunn MG  Tria AJ  Kato YP  Bechler JR  Ochner RS  Zawadsky JP  Silver FH 《Veterinary surgery : VS》1993,22(1):56-56

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Assessment of tetracycline and Babesia culture supernatant as prophylactics for moderating reactions in cattle to live Babesia and Anaplasma vaccines     

WK JORGENSEN  RE BOCK  TG KINGSTON  AJ DE VOS  SJ WALDRON 《Australian veterinary journal》1993,70(1):35-36

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Mesenteric tear of the distal jejunum as a periparturient complication in a mare     

AJ DART  JR PASCOE 《Australian veterinary journal》1994,71(12):427-428

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