Effects of Pasteurella haemolytica A1 culture supernatant on mechanisms controlling bovine alveolar macrophage oxygen radical production.          下载免费PDF全文

P D Conlon  P E Shewen  S F Donnelly    J P Burger 《Canadian journal of veterinary research》1990,54(2):232-237

Pasteurella haemolytica A1 culture supernatant containing leukotoxin, and modifiers of cyclic nucleotide and arachidonate metabolism, were evaluated for their ability to alter oxygen radical production by pulmonary alveolar macrophages obtained from seven Holstein calves. Calves were sedated, and underwent bronchoalveolar lavage to harvest macrophages, which were then incubated with culture supernatant and/or the drugs and toxins under study, and challenged with opsonized zymosan to induce oxygen radical generation. This was measured by a chemiluminescence technique. Pasteurella haemolytica A1 culture supernatant alone delayed the time to maximum oxygen radical production, although total production was increased. The cyclooxygenase inhibitor indomethacin and the phospholipase inhibitor p-bromophenacyl bromide significantly reduced maximum oxygen radical production, but their effects were diminished in the presence of culture supernatant. Although forskolin markedly inhibited oxygen radical generation, this effect was not altered by culture supernatant. Incubation of macrophages with pertussis toxin had no effect on oxygen radical production, while incubation with cholera toxin did inhibit production. This inhibitory effect was significantly lessened by concurrent incubation with P. haemolytica A1 culture supernatant.  相似文献   

The glycosaminoglycan content of the liver in bovine GM1 gangliosidosis.     

A H Johnson  W J Donnelly  B J Sheahan 《Research in veterinary science》1977,22(2):256-266

Chemical analysis of the livers from four calves with GM1 gangliosidosis was negative for significantly elevated levels of glycosaminoglycans. The chemical findings confirmed morphological studies in which hepatic changes were minimal or absent. The findings were compared with the published evidence for the hepatic storage of glycosaminoglycans in human GM1 gangliosidosis.  相似文献   

Effects of dopamine, norepinephrine and serotonin on secretion of luteinizing hormone, follicle-stimulating hormone and prolactin in ovariectomized, pituitary stalk-transected ewes     

P J Donnelly  R A Dailey 《Domestic animal endocrinology》1991,8(1):87-98

Two experiments were conducted in ovariectomized, pituitary stalk-transected ewes to determine if dopamine (DA), norepinephrine (NE) or serotonin (5-HT) alter secretion of luteinizing hormone (LH), follicle-stimulating hormone (FSH) and prolactin (PRL). In experiment 1, ewes were infused (iv) with saline (control), DA (66 micrograms/kg/min), NE (6.6 micrograms/kg/min) or 5-HT (6.6 micrograms/kg/min). Treatments did not alter pulse frequency, but 5-HT increased (P less than .05) amplitude of pulses of LH and mean concentrations of LH, DA and NE were without effect on basal secretion of LH. DA but not NE or 5-HT decreased (P less than .05) the release of LH in response to gonadotropin hormone-releasing hormone (GnRH, 25 micrograms, im). Concentrations of FSH were not affected by treatments. Secretion of PRL was reduced (P less than .05) by treatment with DA and NE but not 5-HT. Each amine reduced (P less than .05) the release of PRL in response to thyrotropin-releasing hormone (TRH; 3 micrograms, im). In experiment 2, ewes were given DA at doses of 0, 0.66, 6.6 or 66.0 micrograms/kg/min, iv. No dose altered basal LH, but each dose reduced (P less than .05) basal and TRH-induced release of PRL. Key findings from these studies include direct pituitary action for: (1) 5-HT enhanced basal secretion of LH, (2) suppression of GnRH-induced secretion of LH by DA. (3) DA and NE inhibition of PRL secretion, and (4) DA, NE and 5-HT inhibition of release of PRL in response to TRH.  相似文献   

Susceptibility of laboratory rats, hamsters, and mice to wound infection with Staphylococcus aureus     

T M Donnelly  D M Stark 《American journal of veterinary research》1985,46(12):2634-2638

Response of rodents to experimentally induced subcutaneous infection was examined to determine whether laboratory rats used in invasive procedures have a superior ability to withstand wound infection than do hamsters and mice. Rats, hamsters, and mice were injected subcutaneously with 10(9), 10(7), and 10(5) colony-forming units of Staphylococcus aureus. Quantitative counts of viable S aureus from the injection site, bacteriologic cultures of heart blood, and histologic examinations of the subcutaneous tissues were performed. Multiple linear regression of the quantitative data and equality of regression lines among groups were determined. Results indicated that the ability to eliminate bacteria varied between species and depended on the dose injected within each species. Compared with hamsters and mice at all doses, rats eliminated bacteria faster and had the mildest and most rapidly organized inflammatory response after inoculation. Experimental bacteremia developed in 3.7% of all animals evaluated, with no species-specific pattern. The rat was more resistant to localized wound infection with S aureus than were hamsters and mice.  相似文献   

Suspected ehrlichiosis in a gelding in Wales   总被引：1，自引：0，他引：1  

P T McNamee  A P Cule  J Donnelly 《The Veterinary record》1989,124(24):634-635

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Bovine asymmetric hind limb paresis, a presumptive in-utero plant poisoning     

CA BOURKE 《Australian veterinary journal》1996,74(1):42-45

Summary: A new locomotory disturbance of cattle is described. The condition has occurred sporadically since the mid-1980s. Affected herds had all grazed flood plain pastures in a restricted area of north-western New South Wales. Calves were either born with clinical signs or developed them by 4 months of age. The disease was characterised by a slowly progressive, irreversible, asymmetrical, paresis of the hind limbs. Affected cattle experienced persistent hyper-extension of the hip and stifle joints. Macroscopic and microscopic examination of the nervous and musculoskeletal systems failed to demonstrate abnormalities that would account for the clinical signs. The disorder shares many similarities with bovine spastic paresis. It is suggested that the pathogenesis of the disorder is nervous, and probably involves nigro-striatal, medulla oblongata, and spinal dysfunctional inputs. An in-utero plant poisoning was suspected but no specific plant association was determined.  相似文献   

Development and trial of a bovine herpesvirus 1 - thymidine kinase deletion virus as a vaccine     

GA SMITH  PL YOUNG†  BJ RODWELL†  MA KELLY†  GJ STORIE†  CA FARRAH†  JS MATTICK† 《Australian veterinary journal》1994,71(3):65-70

SUMMARY An Australian bovine herpesvirus 1 (BHV1) isolate with a defined (427 base pair) deletion in the protein coding region of the thymidine kinase gene was obtained by standard marker rescue procedures. After selection in the presence of the nucleotide analogue 5iodo-deoxy-uridine the virus was analysed by hybridisation with three differential oligonucleotide probes, restriction endonuclease profile studies and DNA sequence analysis. The virus elicited an immune response in recipient animals after either intramuscular or intravenous administration and produced no significant deleterious side-effects when administered at a dose sufficient to stimulate the host immune response. The safety and immunogenicity of the recombinant BHV1 virus 39B1 were similar to those reported for other registered BHV1 vaccines and the virus would appear to be suitable for the production of a vaccine seed lot and more exhaustive field trials as a prelude to commercial vaccine production and registration.  相似文献   

A note on shedding of Salmonella tucson by racing horses     

S.G. Donnelly  L.B. Corbeil   《Preventive veterinary medicine》1983,1(4):383-388

From a sample of 50 clinically normal and 14 diarrheic horses at a racetrack, six of the clinically normal horses from one herd were found to be shedding Salmonella tucson. Shedding was found in horses which were in close contact with poultry: five of the six horses shedding S. tucson had raced recently.  相似文献   

Bovine GM1 gangliosidosis: An inborn lysosomal disease     

W. J. C. Donnelly  B. J. Sheahan 《Veterinary research communications》1977,1(1):65-74

The results of studies on GM₁ gangliosidosis of Friesian calves are reviewed in the light of present knowledge about inborn lysosomal diseases. Affected calves have poor growth rates and develop signs of incoordination and blindness at about three months of age. The disease is progressive resulting in death at six to nine months of age. Storage of GM₁ ganglioside in neurons is associated with reduced activity of tissue -galactosidase. Evidence is presented suggesting an autosomal recessive mode of inheritance. The implications of these findings relative to diagnosis and control are discussed.

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Kurzfassung Die Resultate die bei Studien über die GM₁ Gangliosidose von Friesischen Kälbern erreicht wurden, werden unter dem Gesichtspunkt der aktuellen Kenntnisse über angeborene lysosomale Krankeiten überprüft. Die angegriffenen Kälber haben geringe Wachstumsraten and weisen im Alter von ungefähr 3 Monaten Anzeichen von Nicht-Koordination und Blindheit auf. Bei 6 bis 9 monatigen Kälbern führt die Krankheit zunehmend zum Tode. Die Lagerung von GM₁ Ganglioside in den Neuronen ist mit einer verminderten Aktivät der Gewebe -Galaktosidose verbunden. Der Augenschein deutet auf einen rezessiven autosomalen Vererbungsmodus. Die Folgen dieser Befunde auf Diagnosen und Kontrollen werden diskutiert.

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Resume Les résultats obtenus par les études sur la GM₁ gangliosidose des veaux de race frisonne sont revus sous l'aspect des connaissances présentes sur les maladies lysosomiales innées. Les veaux malades présentent de faibles taux de croissance et des signes de non-coordination et de cécité à l'âge d'environ trois mois. Le taux de mortalité va en croissant de six à neuf mois. Le dépôt de GM₁ ganglioside dans les neurons va de pair avec une réduction de l'activité -galactosidase tissulaire. Il y a lieu de penser qu'il s'agit d'un mode éréditaire autosomal récessif. Les conséquences de ces constatations relatives à la diagnose et au contrôle sont discutées.

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Riassunto I risultati ottenuti tramite gli studi della GM₁ gangliosidosi nei vitelli di razza frisona sono stati riveduti alla luce delle attuali conoscenze delle malattie lisosomali innate. I vitelli ammalati presentano un debole tasso di crescita e segni di non-coordinazione e di cecità verso i tre mesi di età. La mortalità va crescendo dai sei ai nove mesi. L'accumulo di GM₁ ganglioside nei neuroni è associato ad una attività ridotta della -galattosidosi tissulare. E lecito supporre che ci sia presenza di un modo autosomatico recessivo ereditario. Sono discusse le conseguenze di questi risultati in ordine alla diagnosi e al controllo.

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Effects of manipulations to detect sliding hiatal hernia in dogs with brachycephalic airway obstructive syndrome          下载免费PDF全文

Olivier Broux DVM  Cécile Clercx DVM  PhD  Dipl. ECVIM‐CA  Anne‐Laure Etienne DVM  PhD  Valeria Busoni DVM  PhD  Dipl. ECVDI  Stéphanie Claeys DVM  PhD  Dipl. ECVS  Annick Hamaide DVM  PhD  Dipl. ECVS  Frédéric Billen DVM  PhD  Dipl. ECVIM‐CA 《Veterinary surgery : VS》2018,47(2):243-251

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