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11.
Objective To survey finches in pet shops for Cochlosoma infection and evaluate the efficacy of antiprotozoal therapy with metronidazole or ronidazole.
Design A survey of pet shop finches and drug efficacy trials.
Procedure Finches in pet shops were randomly selected and their faeces examined microscopically for motile Cochlosoma sp trophozoites. Drug trials were carried out on 60 adult finches with naturally occurring infections. Body weight was measured and the faeces of each bird was examined for trophozoites at the beginning and 7 days after the end of treatment. In some birds, additional daily faecal examinations were done until three consecutive negative results were obtained. Metronidazole was administered at various dose rates by crop gavage or in drinking water to eight groups of five to ten finches each. Ronidazole was given in water for 7 days to ten finches. In addition, six finches whose faeces tested positive were necropsied and their tissues collected for histological examination.
Results Motile flagellates in the faeces were identified as C anatis -like protozoa. Red-headed parrot-finches, Bengalese and Lady Gould finches were found to be most commonly infected. Cochlosoma sp was also found in the blue-faced parrot-finch, zebra finch, painted finch, nutmeg mannikin and double-barred finch. Metronidazole and ronidazole were found to be effective against Cochlosoma sp. Histological findings on infected adult finches were normal, except for the presence of numerous flagellates between the colorectal villi and cloacal mucosal folds.
Conclusions Cochlosoma anatis -like organisms can infect several species of finches and in adult finches are confined to the colorectum and cloaca. Infection in adult finches was mostly subclinical and could be treated effectively with metronidazole or ronidazole.  相似文献   
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Potassium guaiacolsulfonate, a highly polar, acidic substance, is readily eluted by chloroform from a pH 4.5 Celite column in the form of its ion-pair with trihexylamine, thereby effecting facile separation from other pharmaceuticals. The sulfonic acid is then back-extracted into aqueous alkali and determined spectrophotometrically. Assay of standard solutions by this procedre averaged 100.44 plus or minus 0.81%. The method was applied successfully to 4 commercial cough preparations containing a variety of other drugs.  相似文献   
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Summary The deformation processes involved in wedge indentation of wood are described. The behaviour of wood is compared to that of an elastic-plastic solid and that of a cellular body. It approximates towards the former when its density is high and towards the latter when its density is low. The hardness of wood is examined as a function of density and of wedge angle.We wish to thank the University Grants Committee and the New Zealand Forest Service for supporting this work  相似文献   
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“Indirect selection” is exerted on a trait indirectly, by means other than artificial selection for the trait itself. Expressions are derived for calculating the intensity of indirect selection in aquaculture environments. The calculations are illustrated with growth-rate data on M. rosenbergii from farm ponds in Thailand, and with computer-generated data which simulate measurements made during a multiple mark-recapture experiment. Indirect selection for growth is probably negligible in Thai prawn farms and hatcheries at present, but small changes in management practice could exert strong indirect selection on growth rate. One very effective change appears to be collecting broodstock as early in the production cycle as possible. Control of indirect selection may be useful for the genetic improvement of aquaculture stock, especially in developing countries and other situations where an intensive artificial selection program is not economically or biologically desirable. Like all selection programs, the probability of success is critically dependent on the genetics of the traits being selected.  相似文献   
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Trypanosoma brucei grew in the presence of bovine fibroblast-like cells in Hepes-buffered RPMI 1640 medium with 20 percent fetal bovine serum for more than 220 days at 37 degrees C. The organisms grown in this system were infective to mammalian hosts, retained the morphological and biochemical characteristics of long slender bloodstream forms, and displayed variant-antigen on their surfaces.  相似文献   
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