Column chromatographic analysis of barbiturates in their dosage forms. IV. Combinations with parabens.     

S Barkan  T D Doyle 《Journal of the Association of Official Analytical Chemists》1977,60(5):1022-1029

The nature of the alkaline hydrolysis of some barbiturates in combinations with parabens (p-hydroxybenzoates) was studied with controlled variables, including temperature, viscosity, and concentrations of sodium hydroxide, barbiturate, and parabens. The kinetic studies showed that parabens could be completely hydrolyzed in strong base at 40 degrees C in 1 hr, while the barbiturate remained intact and was readily isolated by partition chromatography, Based on the theoretical results, a partition chromatographic procedure for butabarbital with parabens was devised. Standard recoveries averaged 100.7% with a standard deviation of 0.89. Kinetic data indicate that the hydrolysis of parabens could also be applied to analyze combinations with amo-, seco-, and pentobarbitals. Phenobarbital and parabens are readily separated by partition chromatographic methods without prior hydrolysis of the parabens. The low extraction constant for phenobarbital allowed its retention on a column against relatively strong solvents while the intact parabens are eluted. A slightly modified method was applied to the separation of phenobarbital from parabens. Standard recoveries average 99.9% with a standard deviation of 0.69.  相似文献   

Identification of small molecule activators of cryptochrome   总被引：1，自引：0，他引：1  

T Hirota  JW Lee  PC St John  M Sawa  K Iwaisako  T Noguchi  PY Pongsawakul  T Sonntag  DK Welsh  DA Brenner  FJ Doyle  PG Schultz  SA Kay 《Science (New York, N.Y.)》2012,337(6098):1094-1097

Impairment of the circadian clock has been associated with numerous disorders, including metabolic disease. Although small molecules that modulate clock function might offer therapeutic approaches to such diseases, only a few compounds have been identified that selectively target core clock proteins. From an unbiased cell-based circadian phenotypic screen, we identified KL001, a small molecule that specifically interacts with cryptochrome (CRY). KL001 prevented ubiquitin-dependent degradation of CRY, resulting in lengthening of the circadian period. In combination with mathematical modeling, our studies using KL001 revealed that CRY1 and CRY2 share a similar functional role in the period regulation. Furthermore, KL001-mediated CRY stabilization inhibited glucagon-induced gluconeogenesis in primary hepatocytes. KL001 thus provides a tool to study the regulation of CRY-dependent physiology and aid development of clock-based therapeutics of diabetes.  相似文献   

Eliminating Salmonella in pigs starts with clean feed     

Alan Doyle 《国外畜牧学(猪与禽)》2012,32(3)

欧盟食品安全局(European Food Standards Agency,EFSA)在成功控制养禽业中的沙门氏菌污染后,开始关注养猪业的沙门氏菌污染问题,因为其估计,欧洲大约10％ ～20％的人感染沙门氏菌病例是由食用猪肉产品引起的.  相似文献   

Breeding progress in green curded cauliflowers (Brassica oleracea L. Botrytis group)     

A.R. Gray  L.S. Doyle 《The Journal of Horticultural Science and Biotechnology》2013,88(5):851-859

SUMMARY

Original seedstocks, populations developed by recurrent selection, and inbred lines of two green curded cauliflowers, Di Macerata and Romanesco, were compared in two adjacent but independent trials at Wellesbourne. Trial results indicate that recurrent selection and inbreeding had generally led to significant improvement in uniformity of maturity, increased marketable yield, and improved quality characters contributing to overall appearance and marketability of the product. Depending on the strength of self incompatibility, inbred lines derived from improved populations could potentially be used as varieties, or as parental lines for hybrids.  相似文献   

Pyothorax in a cat managed by intrathoracic debridement and postoperative ventilatory support     

Doyle RS  Bellenger CR  Campoy L  McAllister H 《Irish veterinary journal》2005,58(4):211-215

: A domestic-longhair cat presented due to lethargy, dyspnoea and hypersalivation. Radiographic examination revealed a bilateral pleural effusion, which was diagnosed as pyothorax based on cytological examination. Ultrasonographic examination revealed extensive loculations within the thoracic cavity. Exploratory sternotomy, under general anaesthesia, allowed the removal of approximately 100 ml of purulent fluid and debridement of a partially walled-off abscess and necrotic material from the pleural cavity. Postoperative positive-pressure ventilation was required due to severe respiratory depression. Intensive postoperative care, including intensive continuous monitoring, thoracostomy tube drainage and lavage of the pleural cavity and oesophagostomy tube feeding, was performed. Complete resolution of clinical signs had occurred by 15 days postoperatively. Clinical or radiographic abnormalities were not detected at a follow-up examination one year after surgery.  相似文献   

Report: Feral Pig Workshop 2-4 June 2003     

Doyle K 《Australian veterinary journal》2003,81(8):454

  相似文献   

The Australian Veterinary Reserve and the role of non-government veterinarians     

Kevin Doyle 《Australian veterinary journal》2005,83(7):405-405

  相似文献   

Clinical signs and treatment of chronic uterine torsion in two mares     

Doyle AJ  Freeman DE  Sauberli DS  Hammock PD  Lock TF  Rötting AK 《Journal of the American Veterinary Medical Association》2002,220(3):349-53, 323

Two mares were evaluated because of chronic uterine torsions of 2 and 4 weeks' duration; both were pyrectic, anemic, tachycardic, and anorectic, had signs of depression, and had an abnormal uterus and contents on transrectal examination. Both mares underwent cesarean section for lysis of adhesions from the uterus to the peritoneum, correction of the torsion, and ovariohysterectomy Both mares recovered with only minor complications and returned to be used as riding horses. Chronic uterine torsion should be considered in mares in late stages of gestation that have vague clinical signs and transrectal palpation findings that are unlike those described in typical cases of uterine torsion. Prognosis for life can be good after treatment by ventral midline celiotomy, cesarean section, correction of the torsion, and ovariohysterectomy.  相似文献   

Severity and persistence of footrot in Merino sheep experimentally infected with a protease thermostable strain of Dichelobacter nodosus at five sites     

LJ DEPIAZZI  WD ROBERTS  CD HAWKINS  MA PALMER  DR PITMAN  NC MCQUADE  PD JELINEK  DJ DEVEREAUX  RJ RIPPON 《Australian veterinary journal》1998,76(1):32-38

Objective To test the hypothesis that ovine footrot associated with a thermostable protease strain of Dichelobacter nodosus undergoes self cure or is sustained as an annually recurring disease, depending on the environment.
Design and procedure Forty Merino sheep from a single blood line were infected with a protease thermostable strain of D nodosus a t each of five sites in Western Australia. Footrot lesions and microscopic evidence of D nodosus were recorded every fortnight for 2.5 years, supplemented by laboratory culture. Rainfall, soil and air temperature, pasture quantity and composition and soil types were also recorded. Flocks that apparently self cured were relocated to a more favourable site for footrot in the final spring season.
Results The maximum prevalence of feet with clinical footrot lesions was 80.6, 1.3, 14.4, 3.8 and 88.1% at the five sites. Severe footrot occurred for three consecutive spring seasons at one site that had clay loam soil and at least 3500 kg/ha total pasture dry matter annually. However, the infection was asymptomatic for up to 10 weeks between outbreaks. D nodosus was isolated from flocks for 2.5 years at only two sites, although there was microscopic evidence of the organism at other sites in the final year. A thermolabile variant (strain U6) of D nodosus was isolated from the two sites where footrot persisted.
Conclusion Depending on time and location, ovine footrot induced by a protease thermostable strain of D nodosus either self cured or persisted as annual outbreaks interspersed with periods of asymptomatic infection.  相似文献   

Genetic changes in Oreochromis shiranus (Trewavas) associated with the early stages of national aquaculture development in Malawi   总被引：2，自引：0，他引：2  

A. J. D. Ambali  R. W. Doyle  & D. I. Cook 《Aquaculture Research》1999,30(8):579-588

A study was carried out to investigate the genetic diversity during domestication of Oreochromis shiranus (Trewavas) and to see if it could be associated with events in the known history of aquaculture development in Malawi. Five polymorphic microsatellite loci were scored in 14 populations of O. shiranus and one population of O. mossambicus (Peters). The mean number of alleles per locus ranged from 4.4 ± 1.03 to 13.2 ± 3.31 and was higher in the wild populations than in the domesticated populations. Other measures of genetic diversity were also lower in the domesticated compared with the wild populations, and the decline in diversity was correlated with the time elapsed since the founding of the farm stocks. Ordination analysis grouped domesticated populations into three: (1) those that trace their genealogy from Lakes Chiuta and Chilwa populations and are now spread all over the country; (2) those that come from Lakes Malawi and Malombe; and (3) hybrids between O. shiranus and O. mossambicus. Genetic differentiation among farms was strongly influenced by the pattern of known exchanges among the farmers and introgressive hybridization that had occurred between O. shiranus and O. mossambicus in the farmers’ ponds. Thus, the process of genetic changes in the species subsequent to domestication are best explained and predicted by socio-economic factors that influence the behaviour of farmers, rather than by the time-and-distance models of standard population genetics.  相似文献