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171.
Blank I Pascual EC Devaud S Fay LB Stadler RH Yeretzian C Goodman BA 《Journal of agricultural and food chemistry》2002,50(8):2356-2364
The stability of the coffee flavor compound furfuryl mercaptan has been investigated in aqueous solutions under Fenton-type reaction conditions. The impact of hydrogen peroxide, iron, ascorbic acid, and ethylenediaminetetraacetic acid was studied in various combinations of reagents and temperature. Furfuryl mercaptan reacts readily under Fenton-type reaction conditions, leading to up to 90% degradation within 1 h at 37 degrees C. The losses were lower when one or more of the reagents was omitted or the temperature decreased to 22 degrees C. Volatile reaction products identified were mainly dimers of furfuryl mercaptan, difurfuryl disulfide being the major compound. In addition, a large number of nonvolatile compounds was observed with molecular masses in the range of 92-510 Da. The formation of hydroxyl and carbon-centered radicals was indicated by electron paramagnetic resonance spectra using alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone or 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide as spin traps. Whereas *OH was generated by Fenton-type reactions, the C-centered radical is probably a secondary product of the reaction of *OH with various organic molecules, the reaction with furfuryl mercaptan appearing to be the most important. No evidence for S-centered radicals was seen in the spin-trapping experiments, but a sulfur-containing radical was detected when measurements were made at 77 K in the absence of spin traps. 相似文献
172.
2,4-Dichlorophenoxyacetic acid metabolism in transgenic tolerant cotton (Gossypium hirsutum) 总被引:3,自引:0,他引:3
Laurent F Debrauwer L Rathahao E Scalla R 《Journal of agricultural and food chemistry》2000,48(11):5307-5311
The metabolic fate of 2,4-dichlorophenoxyacetic acid (2,4-D) was studied in leaves of transgenic 2,4-D-tolerant cotton (Gossypium hirsutum), which is obtained by transfer of the tfdA gene from the bacterium Alcaligenes eutrophus. The tdfA gene codes for a dioxygenase catalyzing the degradation of 2,4-D to 2, 4-dichlorophenol (2,4-DCP). [phenyl-(14)C]-2,4-D was administered by petiolar absorption followed by an 18 h water chase or converted to the isopropyl ester and sprayed onto the leaf surface; the leaves were harvested 48 h later. The herbicide was degraded to 2,4-DCP by the bacterial enzyme expressed in the plants. 2,4-DCP was rapidly converted to more polar metabolites and was never found in detectable amounts. Metabolite structures were deduced from enzymatic hydrolysis studies and mass spectrometric analyses. The first metabolite was the glucoside conjugate of 2,4-DCP (2, 4-DCP-beta-O-glucoside). The major terminal metabolites were two more complex glucosides: 2,4-DCP-(6-O-malonyl)glucoside and 2, 4-DCP-(6-O-sulfate)glucoside. 相似文献
173.
Influences of recovery from clear-cut, climate variability, and thinning on the carbon balance of a young ponderosa pine plantation 总被引:1,自引:1,他引:1
Laurent Misson Jianwu Tang Ming Xu Megan McKay Allen Goldstein 《Agricultural and Forest Meteorology》2005,130(3-4):207-222
From 1999 to 2002, the variations in carbon flux due to management practices (shrub removal, thinning) and climate variability were observed in a young ponderosa pine forest originated from clear-cutting and plantation in 1990. These measurements were done at the Blodgett Forest Ameriflux site located in the Sierra Nevada Mountains of California. Thinning in spring 2000 decreased the leaf area index (LAI) by 34% and added 496 g C m−2 of wood and leaf debris at the soil surface. Total ecosystem respiration was not significantly affected by thinning (1261 g C m−2 in 1999 and 1273 g C m−2 in 2000), while canopy photosynthesis decreased by 202 g C m−2. As a result the ecosystem shifted from a net sink of CO2 in 1999 (−201 g C m−2) to a small net source in 2000 (13 g C m−2). Woody and leaf debris resulting from thinning only accounted for maximum 1% and 7% of the total respiration flux, respectively. Thinning did not affect the relative proportion of the different components of respiration to an observable degree. Low soil water availability in summer 2001 and 2002 decreased the proportion of soil respiration to the total respiration. It also imposed limitations on canopy photosynthesis: as a result the ecosystem shifted from a sink to a source of carbon 1 month earlier than in a wetter year (1999). The leaf area index and biomass of the stand increased rapidly after the thinning. The ecosystem was again a sink of carbon in 2001 (−97 g C m−2) and 2002 (−172 g C m−2). The net carbon uptake outside the traditionally-defined growing season can be important in this ecosystem (NEE = −50 g C m−2 in 2000), but interannual variations are significant due to differences in winter temperatures. 相似文献
174.
Grape seed extract affects proliferation and differentiation of human intestinal Caco-2 cells 总被引:2,自引:0,他引:2
Laurent C Besançon P Auger C Rouanet JM Caporiccio B 《Journal of agricultural and food chemistry》2004,52(11):3301-3308
The effect of daily contact of a grape seed extract (GSE) on Caco-2 cell proliferation and differentiation was investigated. GSE at 400 mg/L was added to Caco-2 cells for 2 h a day after successive incubation in saliva, gastric, and pancreatic media. When applied at the beginning of the cell culture, GSE triggered inhibition of cell growth associated with a possible cytotoxic reaction. On the other hand, when the treatment was applied to confluent cells, treated cells displayed a higher protein content than control cells and a more developed brush border, with taller and denser microvilli. These observations were accompanied by stimulation of alkaline phosphatase activity, especially at day 5 postconfluency, with a 2.2-fold increase in comparison with the control. On the other hand, aminopeptidase N activity was inhibited throughout the differentiation period in GSE-treated cells to reach 28.8% of control cell activity on day 30. GSE did not affect either sucrase-isomaltase activity or cytoplasmic lactate dehydrogenase (LDH) activity, which otherwise appeared to be a good cellular marker. GSE treatment of Caco-2 cells thus inhibited their proliferation from seeding onward and stimulated both proliferation and differentiation after confluency. 相似文献
175.
Summary Experiments to assess variation in the resistance of winter wheat to infection by Microdochium nivale were conducted over two consecutive years. Resistance was evaluated using an agar disk technique to reproduce stem lesions and by spraying a conidial suspension to reproduce head blight symptoms. Significant variation for stem reaction measured as stem lesion area (SLA), and head reaction measured as disease severity (DS) was found in the 33 winter wheat genotypes tested. Data obtained over two years in controlled environment conditions were significantly correlated (r=0.713 for SLA and r=0.738 for DS), whereas field data showed a significant genotype x year interaction for disease severity. Quantitative variation for susceptibility to stem and head infection by M. nivale was found among the 33 genotypes tested. The majority of genotypes expressed moderate susceptibility, with cultivar Goupil being very susceptible to both stem and head infection, and the remainder, Renan, Arminda, Munstertaler and Saint-Johann were the most resistant. Resistance to stem and head to M. nivale were not correlated (r=0.358). 相似文献
176.
Clment Baudin Trhiou Sophie Gibert Paul Sriot Antoine Duni‐Mrigot Laurent Blond 《Veterinary radiology & ultrasound》2020,61(2):157-166
Spontaneous pneumothorax induced by grass awns accounts for 5% of spontaneous pneumothorax and 22.5% of thoracic grass awn cases. Previous studies report limited interest of CT for spontaneous pneumothorax. Aims of this retrospective case series were to describe CT features of this condition and determine if CT features can efficiently localize the perforation site. Dogs that had a spontaneous pneumothorax, CT examination, thoracic surgery, and confirmed lung perforation due to a grass awn were included. Computed tomography studies were reviewed and compared to the surgical findings. In 19 of 22 (86.4%) dogs, the pneumothorax or its recurrence were ipsilateral to the perforation site. The perforation site was identified in 21 of 22 (95.5%) dogs and involved the caudal lobes in 20 of 22 (90.9%) cases. The lateralization and the involved lung lobe corresponded to surgical findings in 21 of 22(95.5%) dogs. The perforation site was characterized as a soft tissue attenuating focus lying against an extensive pleural thickening in 21 of 22(95.5%) dogs. An adjacent defect in the visceral pleura was seen in 13 of 22(59.1%) dogs. A grass awn was seen in 11 of 22(50%) dogs. The pneumothorax distribution and grass awn position consistently indicated the perforation side in this sample of dogs. The comparison with surgical findings suggests CT might be helpful for future presurgical planning of this etiology for pneumothorax. 相似文献
177.
Caroline Benzimra Ilaria Cerasoli Delphine Rault Karine Chalvet-Monfray Eddy Cauvin Laurent Couturier Laure Gatel 《Journal of veterinary science (Suw?n-si, Korea)》2020,21(6)
BackgroundGastroesophageal reflux (GER) has been reported to be a common finding in dogs under general anesthesia.ObjectivesThe aim of this retrospective study was to assess the esophageal and gastric contents in a population of dogs undergoing computed tomographic myelography (myelo-CT) examination and to evaluate the factors influencing the presence of esophageal fluid (gastric content, duration of anesthesia, body position, and intrinsic factors).MethodsEsophageal and gastric contents of 83 non-brachycephalic dogs were retrospectively assessed based on plain and myelo-CT scans. Age, weight, breed, sex, and the time between the 2 computed tomography [CT] scans were included.ResultsEsophageal fluid was present in 19% (16/83) of the animals, and 14% (12/83) and 46% (37/83), respectively, had fluid or food material in their stomachs. The frequency of observing esophageal fluid on myelo-CT scans was significantly increased compared with plain CT scans (p = 0.006). The presence of gastric fluid was significantly associated with an increased frequency of observing esophageal fluid compared to other gastric contents (p = 0.049; odds ratio, 3.1). The presence of esophageal fluid was not correlated with alimentary gastric contents (p = 0.17). Increased body weight and duration of anesthesia were significantly associated with an increased frequency of observing esophageal fluid (p = 0.022, p = 0.021).ConclusionsUnlike alimentary gastric contents, fluid gastric contents were correlated with the presence of esophageal fluid upon myelo-CT. The observation of fluid in the esophagus may be consistent with GER. This study provides data additional to pH monitoring studies of GER and may support previous studies recommending shorter pre-anesthetic fasting periods in dogs. 相似文献
178.
Greg G. Goss Chris M. Wood Pierre Laurent Steve F. Perry 《Fish physiology and biochemistry》1994,12(6):465-477
Marked morphological responses occur in the gills of freshwater rainbow trout in response to experimental acid-base disturbance and these responses play an important role in acid-base correction. Compensated respiratory acidosis induced by 70h exposure to environmental hyperoxia (elevated water PO2) caused a 33% decrease in branchial chloride cell fractional surface area (CCFA). Metabolic alkalosis induced by normoxic recovery (6h) from hyperoxia (72h) caused a 50% increase in CCFA, whereas metabolic alkalosis induced by infusion (19h) of NaHCO3 caused a 70% rise. However, the largest increase (135%) in CCFA was seen in response to infusion (19h) of HCl. NaCl infusion had no effect. A particular goal was to assess the relative importance of changes in CCFA vs. changes in internal substrate (HCO3
–) availability in regulating the activity of the branchial Cl–/HCO3
– exchange system. For each of the experimental treatments, the accompanying blood acid-base status and branchial transport kinetics (Km, Jmax) for Cl– uptake had been determined in earlier studies. In the present study, a positive linear relationship was established between CCFA and JCl–
max in individual control fish in the absence of an acid-base disturbance. By reference to this relationship, observed changes in JCl–
max during metabolic acid-base disturbances were clearly due to changes in both CCFA and internal substrate levels (plasma [HCO3
–]) with the two factors having approximately equal influence. 相似文献
179.
Pierre Laurent Suzanne Dunel-Erb Claudine Chevalier Jacques Lignon 《Fish physiology and biochemistry》1994,13(5):353-370
The aim of this work was to determine the kinetics of the dramatic development of the gill chloride cells (CCs) during adaptation
of the salmonid Oncorhynchus mykiss to an ion-poor environment.
To monitor cell division, the incorporation in the mitotic cell DNA of bromo-deoxyuridine (BrdUrd) was visualized with a monoclonal
antibody. The density of labelled nuclei was used as an index of cellular division (proliferation), concomitantly with morphometry
of phenotypic changes monitored with SEM.
In the filament epithelium, a phase of CC differentiation occurred within 12h after the transfer, followed by a delayed phase
of cell proliferation (48h). In the lamellar epithelium, the present study demonstrates the absence of cell proliferation
after ion-poor water transfer. The conclusion is that proliferation (mitosis) is important in the primary filament whereas
differentiation and migration (from the filament) is the main mechanism for the appearance of CCs on the secondary lamellae.
The present study suggests that cortisol promoted differentiation, but not division, of cells. CCs, presumably premature,
were stained by anti-cortisol monoclonal antibody indicating the presence of cortisol. No mature CCs were stained.
Growth hormone (oGH, ratGH) increased the rate of cell division both in lamellar and filament epithelium. 相似文献
180.
Effects of vegetation type and fire regime on flammability of undisturbed litter in Southeastern France 总被引:1,自引:0,他引:1
Anne Ganteaume Jappiot MarielleLampin-Maillet Corinne Curt ThomasBorgniet Laurent 《Forest Ecology and Management》2011,261(12):2223-2231
To assess the effect of vegetation types and of fire regime on the flammability of dead fuels, samples of litter were collected undisturbed (i.e. keeping the structure of litter layers) in 29 study sites spread over the limestone-derived soils of Provence. The sampling plan comprised the most representative ecosystems of the study area: pure Pinus halepensis stands, mixed pine-oak stands and shrublands. Three classes of litter depth were studied (low, medium and high) to account for the variability existing in the field. Sampling also included the number of fires in each site since 1960 (no fire, 1 or 2 fires and ≥3 fires) and the time interval since the last fire (≤15 years, 15-45 years and >45 years). Flammability experiments were carried out in laboratory using a glowing firebrand and a 10 km h−1 wind. The main variables recorded were: ignition frequency, time-to-ignition, flaming duration, flame rate of spread, flame propagation, mean flame temperature, maximum flame height and rate of consumption.Results showed that the ignition frequency was higher in mixed stands than in pure pine stands whereas the time-to ignition, flaming duration and rate of consumption were the highest in pure pine stands. The maximum flame height and the flame propagation decreased with the increase of the number of fires and the time-to-ignition was the highest when the interval since the last fire was the shortest. Increases in litter depth resulted in increased mean flame temperature, maximum flame height and flame propagation. These results can be explained, in part, by the proportions of the different litter components. 相似文献