Frequency of the severe combined immunodeficiency disease gene among horses in Morocco     

M. PIRO  A. BENJOUAD  N. S. TLIGUI  K. EL ALLALI  M. EL KOHEN  A. NABICH  L. OURAGH 《Equine veterinary journal》2008,40(6):590-591

Severe combined immunodeficiency disease (SCID) of horses is an autosomal, recessive hereditary disease occurring among Arabian or crossbred Arabian horses. The genetic defect responsible was previously identified as a 5‐base pair deletion in the gene encoding the catalytic subunit of the DNA dependant protein kinase (DNA‐PKcs). This study was carried out to determine the frequency of SCID and identify horses carrying the gene for SCID among Arabian and Arabian crossbred stallions and mares in Morocco using a DNA‐based test. Twenty‐one horses were SCID carriers: 14 (7%) Arabians, 6 (4%) Arab‐Barbs and one (3.3%) Anglo‐Arab. After analysing their genealogy, 3 imported stallions were identified that disseminated the mutant gene of DNA‐PKcs in Morocco.  相似文献   

The studies on the blood of dogs; haematological findings in apparently healthy dogs harbouring intestinal parasites; dogs infested with Spirocerca sanguinolenta     

EL HINDAWY MR 《The British veterinary journal》1947,103(5):159-165

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An Outbreak of Enzootic Bovine Leukosis in Upper Egypt: Clinical,Laboratory and Molecular–Epidemiological Studies     

A. ZAGHAWA  D. BEIER  I. H. A. ABD EL‐RAHIM  S. EL‐BALLAL  I. KARIM  F. J. CONRATHS  O. MARQUARDT 《Zoonoses and public health》2002,49(3):123-129

In 1989, 220 Holstein Friesian cattle (212 heifers and eight bulls) were imported from Minnesota, USA, to form a closed dairy herd in Arab El‐Aoumar, Assiut, Upper Egypt. In November 1996, some abnormal signs such as loss of weight, decreased milk yield, external lymphadenopathy and decreased appetite were observed on this farm. Serological screening by enzyme‐linked immunosorbent assay revealed a seroprevalence of antibodies directed against bovine leukaemia virus (BLV) of 37.7% in cattle under 2 years old and of 72.8% in animals more than 2 years old. Diagnosis was confirmed by the detection of BLV proviral DNA using polymerase chain reaction with primers amplifying a fragment of the env gene. Out of 21 tested leucocyte fractions from individual animals, 15 were positive showing a BLV‐specific amplicon of 444 base pairs. Analysis of the amplicons for restriction fragment length polymorphisms and DNA sequencing results allowed the isolates to be typed. Since this was the first recorded case of enzootic bovine leukosis in Upper Egypt, strict quarantine measures were adopted and all serologically positive animals in the herd were culled.  相似文献   

OXIDOREDUCTIVE DEGRADATION OF SOIL MINERALS AND CLAYS D,L-3(3,4-DIHYDROXYPHENYL) ALANINE BY SOIL MINERALS AND CLAYS     

J. MAYAUDON  M. EL HALFAWI  L. BATISTIC 《European Journal of Soil Science》1973,24(2):182-192

The effect of the perhydrol (H₂O₂) treated soils on the decarboxylation rate of d,1-3(3,4-dihydroxyphenyl) alanine (DOPA), as examined by radiorespirometry, is highly significant and well-correlated (r=+0.913) with the clay content. Among the pure Na clays used, Wyoming bentonite had the strongest effect on the DOPA decarboxylation. The reaction was activated by phosphate buffer and completely inhibited by a dithionite treatment. Gas phases like carbon monoxide, hydrogen, or nitrogen do not inhibit the DOPA decarboxylation rate. A chromatographic study of the intermediate products formed during DOPA degradation reveals the presence of 5,6-dihydroxyindole. These results suggest that DOPA degradation by clays proceeds by a sequence of oxidoreductive reactions following the Raper (1927) scheme. The ferric oxides associated with the clay, and not the dissolved oxygen, are the electron acceptors. DOPA is oxidized to eumelanin which has an elementary percentage composition of C = 62.65; N = 11.05; H = 6.30; 0 = 20.  相似文献   

EFFECTS OF ADSORBED CATIONS ON PHYSICAL PROPERTIES OF TROPICAL RED AND TROPICAL BLACK EARTHS     

S. A. EL SWAIFY  S. AHMED  L. D. SWINDALE 《European Journal of Soil Science》1970,21(1):188-198

The liquid limits, degrees of dispersion, and moisture retention of a Tropical Red Earth consisting predominantly of kaolin and iron oxides were independent of the nature of the saturating cation. The same three properties of a Tropical Black Earth consisting predominantly of montmorillonite were dependent upon the nature of the saturating cation, with Na giving the highest values and K the lowest. Ca and Mg gave intermediate and essentially equal values. The effects of the four cations have generally been explained in terms of the balance between attractive and repulsive forces in soil solutions and pastes. Unexpected behaviour due to K saturation has been explained by specific adsorption of K in a manner similar to but weaker than irreversible fixation. Linear regression equations were derived to express the dependence of the three properties on the saturating cations. The regression equations, which were all highly significant, show that the four cations examined were acting independently. Small positive interactions between Na × K, Mg × K, and Ca × K indicated that the effect of K fixation was important only when the Black Earth was nearly saturated with K ions.  相似文献   

Histopathological,Immunohistochemical, Lectinhistochemical and Molecular Findings in Spontaneous Bovine Abortions by Campylobacter fetus     

EL Morrell  CG Barbeito  CA Odeón  EJ Gimeno  CM Campero 《Reproduction in domestic animals》2011,46(2):309-315

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Distribution Pattern(s) of Sperm Protein at 22 kDa (SP22) on Fresh,Cooled and Frozen/Thawed Equine Spermatozoa and Expression of SP22 in Tissues from the Testes and Epididymides of Normal Stallions          下载免费PDF全文

LMJ Miller  EM Woodward  JR Campos  EL Squires  MHT Troedsson 《Reproduction in domestic animals》2015,50(2):275-282

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A survey of public attitudes towards barking dogs in New Zealand     

EL Flint  EO Minot  PE Perry 《New Zealand veterinary journal》2013,61(6):321-327

AIMS: To investigate public attitudes towards barking dogs in New Zealand in order to quantify the extent to which people perceive barking dogs to be a problem, to compare tolerance of barking with that of other common suburban noises, to assess the level of public understanding about the function of barking, to determine risk factors for intolerance of barking and to assess knowledge of possible strategies for the investigation and management of problem barking.

METHODS: A 12-page questionnaire was sent to 2,000 people throughout New Zealand randomly selected from the electoral roll. Risk factors for being bothered by barking were examined using logistic regression analysis.

RESULTS: A total of 1,750 questionnaires were successfully delivered; of these, 727 (42%) were returned. Among respondents, 356/727 (49.0%) indicated that frequent barking during the day would bother them while 545/727 (75.0%) would be bothered by barking at night. Barking and howling were ranked above other suburban noises as a cause of annoyance. Risk factors for being bothered by daytime barking were not being home during the day, not owning a dog, and considering a dog bite to be a serious health risk. Risk factors for being bothered by night-time barking were not being home during the day, marital status, considering dog bites to pose a serious health risk, and having been frightened by a dog. Overall, 510/699 (73%) respondents understood that barking was a form of communication.

Action likely to be taken by 666 respondents hearing frequent barking included notifying and offering to help the owner (119; 17.8%), complaining to the owner (127; 19.1%) or the authorities (121; 18.2%), or doing nothing (299; 48%). Possible responses by 211 dog owners if they had a barking dog included seeking help from dog trainers (59; 28%) or behaviourists (54; 26%), buying an anti-barking device (33; 15%) or getting rid of the dog (20; 10%).

CONCLUSIONS: Barking was considered to be potentially disturbing by respondents to this survey. Attitudes towards barking were most influenced by age, dog ownership, past experience with dogs and attitude towards dog bites. Public understanding of the possible reasons for barking and appropriate methods of managing the behaviour when it becomes a problem could be improved by better education and the provision of information through veterinary clinics and social media.  相似文献   

The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating‐induced endometritis          下载免费PDF全文

CE Fedorka  KE Scoggin  EM Woodward  EL Squires  BA Ball  MHT Troedsson 《Reproduction in domestic animals》2017,52(1):89-96

In the horse, breeding induces a transient endometrial inflammation. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating‐induced endometritis PMIE Select seminal plasma proteins cysteine‐rich secretory protein‐3 (CRISP‐3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro. The objective of this study was to determine whether the addition of CRISP‐3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Six mares classified as susceptible to PMIE were inseminated during four consecutive oestrous cycles with treatments in randomized order of: 1 mg/ml CRISP‐3, 150 μg/ml lactoferrin, seminal plasma (positive control) or lactated Ringer's solution (LRS; negative control) to a total volume of 10 ml combined with 1 × 10⁹ spermatozoa pooled from two stallions. Six hours after treatment, an endometrial biopsy was obtained for qPCR analysis of selected genes associated with inflammation (pro‐inflammatory cytokines interleukin (IL)‐1β, IL‐8, tumour necrosis factor (TNF)‐α, interferon (INF)‐γ, anti‐inflammatory cytokines IL‐1RN and IL‐10, and inflammatory‐modulating cytokine IL‐6). Seminal plasma treatment increased the mRNA expression of IL‐1β (p = .019) and IL‐8 (p = .0068), while suppressing the mRNA expression of TNF (p = .0013). Lactoferrin also suppressed the mRNA expression of TNF (p = .0013). In conclusion, exogenous lactoferrin may be considered as one modulator of the complex series of events resulting in the poorly regulated pro‐inflammatory response seen in susceptible mares.  相似文献   

Rodent control and food supplementation increase productivity of endangered San Clemente Loggerhead Shrikes (Lanius ludovicianus mearnsi)     

Shane R. Heath  Eric L. Kershner  Douglass M. Cooper  Suellen Lynn  David K. Garcelon 《Biological conservation》2008,141(10):2506-2515

Habitat loss and predation by exotic predators have contributed to the decline of many island populations of birds, including San Clemente Loggerhead Shrikes (Lanius ludovicianus mearnsi). This critically endangered subspecies has been the recipient of an extensive and expensive recovery effort, including a captive breeding and release program. We examined the effects of life-history characteristics, rainfall, and management, particularly food supplementation and rat (Rattus rattus) control, on the breeding productivity of 172 San Clemente Loggerhead Shrike pairs from 2000 to 2006. Annual fecundity and the number of fledglings reaching independence per pair varied with year and were closely correlated with prebreeding (November-March) precipitation levels. Annual fecundity increased as a result of management implemented between April and July each year. Shrike pairs receiving no management during this period produced a mean of 1.9 fledglings. Pairs receiving both food and rat control produced an average of 2.5 more fledglings than pairs receiving no management, while pairs receiving rat control alone produced an average of 1.1 more fledglings than pairs receiving no management during this period. Wild-origin females produced an average of 0.8 more fledglings per pair than captive-origin females, while older males produced an average of 0.9 more fledglings and independent young relative to inexperienced males. The effects of food supplementation and rat control on San Clemente Loggerhead Shrikes were most pronounced during dry years, suggesting such management should be emphasized when prebreeding precipitation levels are below historical averages. These techniques offer an effective means to increase breeding productivity of avian species in immediate threat of extinction.  相似文献