Pancreatic biopsy in normal cats     

TA LUTZ  JS RAND  P. WATT  A. GALLOWAY 《Australian veterinary journal》1994,71(7):223-225

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Plasma Antimullerian Hormone as a Predictor of Ovarian Antral Follicular Population in Bos indicus (Nelore) and Bos taurus (Holstein) Heifers     

EOS Batista  GG Macedo  RV Sala  MDDV Ortolan  MF Sá Filho  TA Del Valle  EF Jesus  RNVR Lopes  FP Rennó  PS Baruselli 《Reproduction in domestic animals》2014,49(3):448-452

In Bos taurus cattle, antimullerian hormone (AMH) has been demonstrated to have a high degree of correlation with ovarian antral follicle count and the number of healthy follicles and oocytes. To document the correlation between the plasma concentration of AMH and follicular number in Bos indicus and Bos taurus heifers, Nelore (Bos indicus, n = 16) and Holstein heifers (Bos taurus, n = 16) had their ovarian follicular waves synchronized. After synchronization, ovarian antral follicular population (AFP) was evaluated three times at 60‐day (d) intervals (T‐120 d, 120 days before plasma AMH determination; T‐60 d, 60 days before; and T0, at the time of plasma AMH determination). The plasma AMH concentration was positively correlated with the number of ovarian follicles on the day of the follicular wave emergence in Bos indicus (Nelore) and Bos taurus (Holstein) heifers at each evaluation time (p < 0.05). The AFP was higher in Bos indicus (Nelore) than in Bos taurus (Holstein) heifers (p < 0.05). Similarly, the AMH concentration was higher in Bos indicus (Nelore) than in Bos taurus (Holstein) heifers (p < 0.0001). When heifers were classified as to present high or low AFP according to the mean of the AFP within each genetic group, high‐AFP heifers presented a greater (p < 0.0001) AMH concentration than low‐AFP heifers, regardless of the genetic group. In conclusion, the AFP is positively correlated with plasma AMH concentration in both Bos indicus (Nelore) and Bos taurus (Holstein) heifers. Furthermore, Bos indicus (Nelore) heifers presented both greater plasma AMH concentrations and AFP than Bos taurus (Holstein) heifers.  相似文献   

Analysis for total sulfite in foods by using rapid distillation followed by redox titration     

J W DeVries  H Ge  F J Ebert  J M Magnuson  M K Ogawa 《Journal of the Association of Official Analytical Chemists》1986,69(5):827-830

A rapid and accurate analysis for total sulfite as sulfur dioxide has been developed for foods and food products. The method, which combines a selective distillation cleanup procedure with the selective redox titration of sulfite ion by iodine, has been applied to a variety of foods and food products over a period of time with no significant interference encountered in any matrixes other than garlic and leeks. For the foods analyzed, the method typically shows a detection limit of 10 ppm, a relative standard deviation of 7.5% (compared with 10.4% for similar matrixes by the Monier-Williams method), and recoveries of 97.9 +/- 6.4%. Comparison of results for this method with those obtained using the Monier-Williams method showed a mean value for the distillation/titration method of 241 ppm compared with 242 ppm for the Monier-Williams method. A correlation of 0.991 and odds of a difference between methods of 10.7% (Student's paired t-test (1-alpha) X 100) were obtained for those matrixes where no interferences were encountered with either method.  相似文献   

Centres of Crop Diversity and/or Origin, Genetically Modified Crops and Implications for Plant Genetic Resources Conservation   总被引：1，自引：0，他引：1  

J. M. M. Engels  A. W. Ebert  I. Thormann  M. C. de Vicente 《Genetic Resources and Crop Evolution》2006,53(8):1675-1688

The concept of centres of crop diversity and/or origin of agriculture is briefly reviewed. The conservation status of crop genetic resources, either ex situ or in situ, cultivated or wild, has been assessed for species of the Central American and Mexican centre, demonstrating that that region is indeed one of the important centres of crop diversity for human kind. Furthermore, biotechnological developments with regard to the creation and spread of genetically modified crops have been analyzed. The likelihood of unintentional introgression of genetically modified traits into conventional seed lots, crops as well as into germplasm collections have been assessed. Related biosafety measures as well as the possible implications of intellectual property rights on transgenic crops and/or genes are being discussed vis-a-vis the possible implications they might have for germplasm management. The Central American crop genetic resources situation has been used as a “case study” to illustrate the potential impact of the spreading of GM varieties on the genetic diversity in genebanks and farmers’ fields and the need for effective and efficient conservation efforts. Conservation management strategies and practices are being proposed of mitigate the potential negative impact of GM crops on the conservation efforts.  相似文献   

The role of social groups in the persistence of learned fear     

Olsson A  Ebert JP  Banaji MR  Phelps EA 《Science (New York, N.Y.)》2005,309(5735):785-787

Classical fear conditioning investigates how animals learn to associate environmental stimuli with an aversive event. We examined how the mechanisms of fear conditioning apply when humans learn to associate social ingroup and outgroup members with a fearful event, with the goal of advancing our understanding of basic learning theory and social group interaction. Primates more readily associate stimuli from certain fear-relevant natural categories, such as snakes, with a negative outcome relative to stimuli from fear-irrelevant categories, such as birds. We assessed whether this bias in fear conditioning extends to social groups defined by race. Our results indicate that individuals from a racial group other than one's own are more readily associated with an aversive stimulus than individuals of one's own race, among both white and black Americans. This prepared fear response might be reduced by close, positive interracial contact.  相似文献   

Scanning Single-Electron Transistor Microscopy: Imaging Individual Charges     

MJ Yoo  TA Fulton  HF Hess  RL Willett  LN Dunkleberger  RJ Chichester  LN Pfeiffer  KW West 《Science (New York, N.Y.)》1997,276(5312):579-582

A single-electron transistor scanning electrometer (SETSE)-a scanned probe microscope capable of mapping static electric fields and charges with 100-nanometer spatial resolution and a charge sensitivity of a small fraction of an electron-has been developed. The active sensing element of the SETSE, a single-electron transistor fabricated at the end of a sharp glass tip, is scanned in close proximity across the sample surface. Images of the surface electric fields of a GaAs/AlxGa1-xAs heterostructure sample show individual photo-ionized charge sites and fluctuations in the dopant and surface-charge distribution on a length scale of 100 nanometers. The SETSE has been used to image and measure depleted regions, local capacitance, band bending, and contact potentials at submicrometer length scales on the surface of this semiconductor sample.  相似文献   

Costing the ex situ conservation of plant genetic resources at AVRDC – The World Vegetable Center     

Pepijn Schreinemachers  Andreas W. Ebert  Mei-Huey Wu 《Genetic Resources and Crop Evolution》2014,61(4):757-773

AVRDC – The World Vegetable Center maintains the world’s largest international public collection of vegetable genetic resources at its headquarters in Taiwan. The ex situ conservation and dissemination of germplasm to researchers and breeders worldwide contributes to global food and nutrition security but also carries considerable costs. The objective of this study is to quantify these costs for the 12-month period from September 2011 to August 2012 using the Decision Support Tool developed by the International Food Policy Research Institute. The results show that the present value of capital assets is USD 1.99 million for the facilities and USD 0.48 million for the equipment. The total annual cost is USD 0.684 million, of which 74 % are labor costs. The average conservation and dissemination cost per accession is USD 10.08 per year. Seed regeneration, seed processing, characterization, and seed dissemination are the four most costly operations of the genebank. The storage itself only contributes 17 % of the cost. In comparison, the average cost per accession is USD 5.15 at ICARDA, USD 6.84 at CIMMYT, USD 8.62 at ICRISAT, USD 9.19 at IRRI, and USD 22.52 at CIAT (in 2012 US dollar values). High labor costs in Taiwan increase AVRDC’s average cost, but the fact that more vegetable species are self-pollinating and thus less labor intensive to regenerate than cross-pollinating species keeps the average costs in check. These results are important benchmarks for other genebanks.  相似文献   

冀西北坝上地区大蒜播种期研究     

黄伟  李文杰  塔秀成  苗丁丁  李刚 《湖北农业科学》2017,56(13)

为了探讨冀西北坝上地区大蒜适宜的播种期,给当地大蒜高效生产提供理论依据,试验以大蒜品种西八里为试验材料,设置A(3月22日)、B(4月1日)、C(4月11日)3个不同播期,测定了不同播期下大蒜的生育期、植株性状、蒜头性状、蒜薹和蒜头产量等指标。结果表明,播期B和C较播期A的出苗时间分别缩短7 d和11 d;播期B的植株性状、蒜头性状均优于播期A和C,播期B一级蒜的比例最高,较播期A、C分别高出11.0和27.6个百分点;播期B较播期A和C的蒜薹产量分别提高8.41%和40.82%,蒜头产量分别提高10.19%和16.08%。本试验条件下,坝上地区大蒜适宜的播种期为3月底至清明节之间。  相似文献   

西北旱区生态服务价值时空动态研究   总被引：1，自引：0，他引：1  

田龙  张青峰  塔娜  章慧  董艳 《干旱地区农业研究》2017,35(6):227-234

为探明西北旱区生态服务价值的时空差异性,以2004—2012年相关数据为基础,对其生态服务价值进行计算。结果表明:(1)2012年西北旱区的生态服务价值较2004年增加了166.1×109元,而人均生态服务价值却减少了8元,说明人口的增速比生态服务价值的增速大,应适当控制人口增长;(2)西北旱区各省区生态服务价值变化大小依次为甘肃陕西中北部新疆内蒙古中西部青海宁夏,其中宁夏和青海为负变化;(3)2004—2012年间西北旱区生态经济协调度为0.33,表明生态环境和经济发展处于轻度协调状态;(4)敏感性指数小于1,说明西北旱区生态服务价值对生态价值系数弹性较弱,本文研究结果真实可信。研究期内的土地利用变化取得了较明显的生态效益,为西北旱区的经济建设和社会发展提供了良好的环境平台。  相似文献   

猪流行性腹泻病毒S2基因B细胞表位嵌入型S-层蛋白在副干酪乳杆菌中的表达与鉴定     

白伟琴  卡楚拉  乌志勇  苗苗  塔娜  格日勒图 《中国畜牧兽医》2022,49(10):4004-4011

【目的】构建新型猪流行性腹泻病毒(Porcine epidemic diarrhea virus, PEDV)乳酸菌活菌疫苗载体。【方法】应用DNA重组技术将嗜酸乳杆菌S-层蛋白(SLP)基因及PEDV S2基因B细胞表位(EpitopeS2)融合基因(SLP-EpitopeS2)克隆到乳酸杆菌表达载体pTRK892中,构建重组载体pTRK-SLP-EpitopeS2,通过电转化方法将重组质粒导入副干酪乳杆菌中,获得重组副干酪乳杆菌。分别用SDS-PAGE、Western blotting和免疫荧光试验(IFA)鉴定目的蛋白在副干酪乳杆菌中的表达。【结果】PCR结果显示,成功扩增出大小为1 400 bp的目的条带,与插入融合基因大小一致,双酶切结果出现大小分别为1 400和4 700 bp的2条带,基因测序结果显示无碱基缺失和突变等,从而确定重组质粒pTRK-SLP-EpitopeS2构建正确。SDS-PAGE、Western blotting结果显示,在48 ku处出现与理论值大小一致的目的蛋白条带,表明融合基因SLP-EpitopeS2在副干酪乳杆菌中得到有效表达。IFA结果显示,...  相似文献