Construction and immunogenicity studies of recombinant fowl poxvirus containing the S1 gene of Massachusetts 41 strain of infectious bronchitis virus   总被引：9，自引：0，他引：9  

Wang X  Schnitzlein WM  Tripathy DN  Girshick T  Khan MI 《Avian diseases》2002,46(4):831-838

The spike 1 (S1) surface glycoprotein of infectious bronchitis virus (IBV) is the major inducer of the generation of virus neutralizing antibodies, and the administration of purified S1 has been shown to elicit a protective immune response against virulent virus challenge. On the basis of these observations, recombinant fowl poxvirus (rFPV) containing a cDNA copy of the S1 gene of IBV Mass 41 (rFPV-S1) was constructed and its immunogenicity and vaccine potential were evaluated. Initially, rFPV-S1 was shown to express the S1 in vito by indirect immunofluorescence staining and western blot analyses. Later, in vivo expression was demonstrated by the detection of IBV-specific serum immunoglobulin G and neutralization antibodies in the sera of chickens immunized with rFPV-S1. That the recombinant virus elicited anti-IBV protective immunity was indicated by the manifested, relatively mild clinical signs of disease, decreased titers of recovered challenge virus, and less severe histologic changes of the tracheas in virulent IBV Mass 41-challenged chickens previously receiving rFPV-S1 as compared with parental fowl poxvirus (FPV)-vaccinated control birds. In contrast, chickens immunized with either recombinant or parental FPV were resistant to a subsequent virulent FPV challenge. As to a preferred method of immunization, wing web administration appeared to be superior to the subcutaneous route because a greater percentage of birds vaccinated by the former protocol exhibited an anti-IBV humoral immune response. Thus, rFPV-S1 has potential as a poultry vaccine against both fowl pox and infectious bronchitis.  相似文献   

Diagnosis by polymerase chain reaction of Erysipelas septicemia in a flock of ring-necked pheasants     

Hennig GE  Goebel HD  Fabis JJ  Khan MI 《Avian diseases》2002,46(2):509-514

A flock of 810 pheasants experienced 6.2% mortality over 6 days. Affected birds were weak and lethargic for up to 24 hr before death. Examined birds were thin, and gross lesions consisted of thick opaque crops and cecal cores. Histologically, there was capillariasis of the crop and multifocal ulcerative typhlitis with Heterakis spp. infection, and numerous systemic intravascular monocytes were filled with clusters of blue rod-shaped organisms. The organisms were gram-positive bacilli by Brown and Brenn staining and ultrastructural analysis. Liver bacterial cultures were negative for pathogenic bacteria. Erysipelas septicemia was diagnosed by an Erysipelothrix species-specific polymerase chain reaction method with the substrate DNA isolated from formalin-fixed, paraffin-embedded liver.  相似文献   

Clinical syndrome associated with zolpidem ingestion in dogs: 33 cases (January 1998-July 2000)     

Richardson JA  Gwaltney-Brant SM  Albretsen JC  Khan SA  Porter JA 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(2):208-210

Zolpidem is a nonbenzodiazepine hypnotic of the imidazopyridine class that is used to treat insomnia in humans. Zolpidem binds selectively to the benzodiazepine omega-1 receptor and increases the frequency of chloride channel opening, which results in inhibition of neuronal excitation. A retrospective study was conducted of zolpidem ingestion in dogs that were reported to the ASPCA Animal Poison Control Center (APCC) between January 1998 and July 2000. Data analysis included amount ingested, clinical effects, and time of onset of signs. Thirty-three reports of zolpidem ingestion in dogs (ranging in age from 5 months to 16 years) were evaluated. Approximate ingested dosages ranged from 0.24 to 21 mg/kg. Clinical signs reported included ataxia (18 dogs; 54.5%), hyperactivity (10 dogs; 30.3%), vomiting (7 dogs; 21.2%), and lethargy (5 dogs; 15.2%), as well as panting, disorientation, nonspecific behavior disorder, and hypersalivation (4 dogs each sign; 12.1%). Other signs reported include tachycardia, tremors, apprehension, vocalization, hypersalivation, weakness, and hyperesthesia. In 85% percent of reports, clinical signs developed within 1 hour and usually resolved within 12 hours. Although central nervous system (CNS) depression is reported as a primary effect of zolpidem in humans and would also be expected in dogs, information obtained from this study indicates that some dogs may exhibit a paradoxical excitation reaction. This effect appears to vary among individual dogs.  相似文献   

Ability of induced corpora lutea to maintain pregnancy in beef cows   总被引：1，自引：0，他引：1  

Bridges PJ  Wright DJ  Buford WI  Ahmad N  Hernandez-Fonseca H  McCormick ML  Schrick FN  Dailey RA  Lewis PE  Inskeep EK 《Journal of animal science》2000,78(11):2942-2949

Experiments were conducted in beef cows without a primary CL, in which pregnancy had been maintained with exogenous progestogen. In preliminary trials, replacement CL induced ipsilateral to the embryo and after, rather than before, d 36 of pregnancy, maintained more pregnancies after withdrawal of exogenous progestogen (13/13 vs 2/6; P < 0.05). In Exp. 1, in cows with replacement CL induced by treatment with hCG on d 28 of pregnancy, treatment with flunixin meglumine on d 31 through 37 did not increase maintenance of pregnancy. Experiment 2 was conducted to evaluate directly the effects of concentrations of PGF2alpha and estradiol-17beta during d 31 through 35 of pregnancy on maintenance of pregnancy by replacement CL induced between d 28 and 31. In cows that maintained pregnancy while progestogen was provided, maintenance of pregnancy after withdrawal of exogenous progestogen tended to be greater with high (5/5) than with low (2/6; P < 0.10) concentrations of PGF2alpha and greater with low (6/7) than with high (2/6; P = 0.10) concentrations of estradiol-17beta. Secretion of progesterone by replacement CL was greater (P < 0.05) in cows with high than in those with low concentrations of PGF2, during d 31 through 35. Prostaglandin F2alpha may facilitate attachment of the bovine embryo (d 30 to 40) in a manner similar to that reported for implantation in other species. Cows that did not form CL in response to hCG on d 28 to 31 responded well when retreated after d 36. Again, maintenance of pregnancy was greater when replacement CL were induced after (9/9) rather than before d 36 (8/16; P < 0.05).  相似文献   

肉鸡日粮电解质平衡与热应激的关系     

T.Ahmad  M.Sarwar  吴云良 《中国家禽》2007,29(16):28-31

在家禽业中,缓解热应激的方法涉及饲养管理和改变日粮配方等.管理制度方面如提高鸡舍隔热效果,有流畅的通风设施,屋顶安装喷水装置和蒸发制冷系统,使用鼓风机,降低饲养密度.……  相似文献   

Hybridization and in vitro culture of an orchid hybrid Ascocenda ‘Kangla’     

Rajkumar Kishor  P.S. Sha Valli Khan  G.J. Sharma 《Scientia Horticulturae》2006

The present study focuses on hybridization program involving two species belonging to two different vandaceous genera, viz., Ascocentrum ampullaceum (Roxb.) Schltr. var. auranticum, a narrow endemic orchid of Manipur and Vanda coerulea Griff., an endangered orchid of Appendix I of CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora), to synthesize the primary hybrid genus with intermediate and improved characters in the F₁ generation. Observations on the crossability in the present bigeneric cross between V. coerulea and A. auranticum had been achieved with 60% success when V. coerulea was taken as female parent. Murashige and Skoog (MS) basal medium at half-strength was effective for the development of the hybrid seedlings of V. coerulea × A. auranticum followed by Vacin and Went (VW) and Knudson C (KC) media. The best response of seedling growth was observed on MS medium at half-strength supplemented with 2.3 μM kinetin + 0.5 μM α-naphthalene acetic acid with maximum shoot height (2.7 cm), leaf number (4.6) and root number (4.1) after 150 days of inoculation. The survival percentage and growth performance of the seedlings were found to be higher (80% survival) in potting substrate consisting of brick:charcoal in the ratio 2:1 mulched with moss (Sphagnum sp.) than in potting substrate consisting of brick:charcoal:tree fern in the ratio 2:1:1. The first flowering was observed in the hybrid seedlings of V. coerulea × A. auranticum after 2 years of transfer to the ex vitro environment. Morphologically, the flowers differed from that of the parents clearly showing the success of the hybridization experiment. Registration of the hybrid has been made with the Royal Horticultural Society with the nomenclature Ascocenda ‘Kangla’ (No. T128725).  相似文献   

Effect of root applied 28-homobrassinolide on the performance of Lycopersicon esculentum     

B. Ali  S. HayatS. Aiman Hasan  A. Ahmad 《Scientia Horticulturae》2006

The roots of 20 days old seedlings of tomato (Lycopersicon esculentum Mill.) at the time of their transplantation, were dipped in 10⁻⁸, 10⁻⁷, 10⁻⁶ or 10⁻⁵ M of 28-homobrassinolide (HBR) for 15, 30 or 45 min and were allowed to grow in earthen pots, in a net house. The leaves of the plants, at days 30 and 60, possessed elevated quantities of nitrate reductase (NR), carbonic anhydrase (CA) and the contents of chlorophyll. The values for all the above characteristics were significantly higher than that of the water-fed control. The fruits borned at the treated plants were more in number and possessed a lower quantity of ascorbic acid than the control. Moreover, the fruits at ripening, had higher levels of lycopene and β-carotene. Among the treatments, 15 min feeding of 10⁻⁸ M HBR proved best.  相似文献   

Comparative Disposition Kinetics of Albendazole in Sheep Following Oral and Intraruminal Administration     

Swarnkar CP  Sanyal PK  Singh D  Khan FA  Bhagwan PS 《Veterinary research communications》1998,22(8):545-551

The pharmacokinetics of albendazole was studied in sheep following single oral and intraruminal administration at nematocidal dose rates. The disposition curves of its metabolites indicated increased uptake of the drug in sheep following intraruminal as compared to oral dosing (p<0.05). The increased bioavailability of benzimidazole anthelmintics given by the intraruminal route could be exploited for optimizing the use of anthelmintic for sustained parasite control in small ruminants.  相似文献   

Pyrrolizidine alkaloids from Senecio lorenthii.     

M Noorwala  F V Mohammad  V U Ahmad  B Sener  F Ergun  D Deliorman 《Fitoterapia》2000,71(5):618-620

The isolation and NMR spectra of otosenine (1) and seneciphylline (2) from Senecio lorenthii are reported.  相似文献   

Effect of Gibberellic Acid Spray and Basal Nitrogen and Phosphorus on Productivity and Fatty Acid Composition of Rapeseed-Mustard     

N. A. Khan  H. R. Ansari  Samiullah 《Journal of Agronomy and Crop Science》1997,179(1):29-33

Two field experiments were conducted during] 994-95 to study the effect of spray of 10⁻⁵ M GA3 at 40 days after sowing on mustard ( Brassica juncea (L.) Czern & Coss.) cv. Varuna grown with basally applied 0, 40, 80 and 120 kg N ha⁻¹ (Expt. 1) and 0,15, 30 and 45 kg P ha⁻¹ (Expt. 2) on pod number per plant, seeds per pod, 1000 seed weight, seed yield, biological yield, harvest index and fatty acid composition of oil. No significant difference between water and GA3 spray was found when basally applied nitrogen was 0 or 40 kg N ha⁻¹. N₈₀ proved to be the best for yield characteristics. In another experiment on phosphorus, GA₃ and 30 kg P ha⁻¹ individually enhanced the yield, but interaction of GA₃ and P remained non-significant. The fatty acid composition of oil in both experiments was significantly affected only by nitrogen and phosphorus treatments for oleic acid and erucic acid. It was found that return in the form of yield was more for every kg applied fertilizer under GA, spray treatment. The response was more for N fertilizer in comparison to P. GA₃ at a low level of fertilization significantly increased the return from fertilization.  相似文献