Biological characteristics of JAK2 transduced CD34⁺ cells from cord blood during ex vivo expansion     

ZHAO Sheng-ming  GU Xi-chun  CHANG Nai-bai  XU Xiao-dong  PEI Lei 《园艺学报》2007,23(12):2460-2464

AIM: To explore the feasibility and biological characterization of long-term regulated expansion of JAK2 transduced human CD34⁺ cord blood cells in vitro.METHODS: A retrovirus (RV) vector which contains JAK2 catalytic domain and two binding sites for a chemical inducer,dimerization (AP20187),was cloned (designated MGI-F₂JAK2).CD34⁺cells were enriched from cord blood with a MiniMACS system.The purified CD34⁺ cells were transfected with supernatant from the retrovirus packaging cell line that expressed JAK2.Following transduction,cells were expanded into four groups: AP20187 alone,FL alone,TPO,alone,AP20187+FL+TPO,respectively.The expanded cells were monitored by GFP expression,immunophenotyping,progenitor colony assay,karyotype analysis as well as tumorigenesis in nude mice.RESULTS: The purity of selected CD34⁺ cells was over 91% and gene transfer rate was 49.32%±6.21%.Only the group of AP20187 +FL+ TPO was obtained a significant sustained outgrowth of the transduced CD34⁺ cord blood cells.The percentage of GFP+ cells consistently produced a rise to the 90% peak level by the end of 8th week of culture.Flow cytometry analysis showed that the phenotype of the expanded cells was CD33⁺,CD61⁺ and Gly-A⁺ partial positive;CD38⁺ and HLA-DR⁺ strong positive,while CD2,CD7 and CD19 were almost negative.Colony assays performed in methycelluos,which can give rise to BFU-E,CFU-GM and CFU-Mix,the CFU-GM was predominantly in all colonies.The tumor was not observed in nude mice and the karyotype analysis was normal from expanded cells.CONCLUSION: The results demonstrate that AP20187-mediated activation of JAK2 signaling is capable of stimulating expansion JAK2 transduced CB CD34⁺ cells in combination with FL and TPO.This system may have applications for studies in signaling transduction,hematopoiesis,and for gene and cell therapy.  相似文献   

Experimental study on how brain-dead state affects the heart structure and function of Ba-Ma mini pigs and the mechanism     

ZHANG Shui-jun  ZHU Sheng-xing  LI Jie  MA Xiu-xian  FENG Liu-shun  FAN Zheng-jun 《园艺学报》2007,23(8):1480-1483

AIM:To investigate how brain-dead state affects the heart structure and function and the effect of PKC-α in BA-Ma mini pigs.METHODS:Ten Ba-Ma mini pigs were randomized into 2 groups: brain-dead group (n=5),and control group (n=5). The brain-dead model was made by increasing intracranial pressure,while the control group was maintained anesthesia for 24 h. The concentrations of cTnT,TNF-α,IL-1β and IL-6 in serum were determined at 6,12 and 24 h after brain death. At 24 h,heart tissues were observed by HE staining and electron microscope. The expression of PKC-α was detected by immunohistochemistry and RT-PCR.RESULTS:(1) Histological changes of myocardium: flaky bleeding under endocardium and dissolution of myocardium were found in optical microscope. In electron microscope dropsical mitochondria and confluent muscle fiber were found. (2) Changes of serum cTnT: serum cTnT for brain-dead group began to increase gradually since 6 h,and were significantly higher at each time point than those in control group (P<0.05). (3) Changes of inflammatory factors: IL-1β,IL-6,and TNF-α in brain-dead group began to increase gradually since 6 h,and were significantly higher at each time point than those in control group (P<0.05). (4) Changes of PKC-α expression: PKC-α mRNA and protein expressions in brain-dead group increased significantly at 24 h (P<0.05).CONCLUSION:Brain death may evoke heart structure and functional injury,and increase the levels of inflammatory factors and PKC-α. The activation of PKC-α may participate in the process of heart injury.  相似文献   

扁穗牛鞭草种质遗传多样性的ISSR分析   总被引：2，自引：10，他引：2  

范彦  李芳  张新全  马啸 《草业学报》2007,16(4):76-81

用ISSR标记对来自中国西南地区(四川、重庆、贵州)的28份扁穗牛鞭草材料的遗传多样性进行了检测。从96个ISSR引物中共筛选出13个多态性明显、反应稳定的引物。28份材料的DNA共扩增出129条谱带,平均每个引物扩增出9.9条带,多态性条带比率达84.2%。材料间遗传相似系数为0.466~0.980,表现出丰富的遗传多样性。通过聚类分析和主成分分析,将28份扁穗牛鞭草分为两大类,同一地区的扁穗牛鞭草品种(系)基本聚在同一类,呈现出一定的地域性分布规律。  相似文献   

红壤柑桔园土壤剖面形态特征及其有效微量元素含量研究     

范西宁  黄玉溢 《广西园艺》2007,18(2):15-16

7个红壤柑桔园土壤剖面形态特征及其26个土样的有效微量元素含量研究表明，柑桔土壤有效土层分化有3个层次，各层次的形态有自已的特征；土壤剖面中有效B、Zn、Mo严重缺乏，部分果园由于长期使用含Cu杀菌剂而使土壤剖面中有效Cu含量过高；有效B、Cu在土壤剖面中分布自上而下存在明显的递减性，而有效Zn、Mn、Mo分布无明显规律。建议在红壤柑桔园上增施B、Zn、Mo肥，控制喷施含Cu杀菌剂。  相似文献   

Influence of the fixation with auto-control micro-movement intramedullary interlocking nail mode on fracture healing in goats     

ZHANG Hong-jun  FAN Shun-wu  XU Wei-zhou  HE Chang-qing 《园艺学报》2007,23(1):178-182

AIM: To investigate the effect of auto-control micro-movement locking nail (AMLN) on experimental fracture healing. METHODS: 32 goats with a transverse osteotomy of the femoral shafts (16 matched pairs) were fixed intramedullary with AMLN and Gross-Kempf (GK), respectively. The follow-up time was 7, 14, 28 and 56 days, histological and biochemical analysis were done. RESULTS: (1) Histological observation and quantitative analysis of calluses revealed that AMLN promoted the growth of bridge calluses and periosteum calluses. In that way the facture healing and remodeling process came along ahead of time that was much better than traditional GK nail fixation. The significant difference between them was observed (P<0.05). (2) The content of the total collagen, insoluble collagen, calcium and phosphate in the AMLN fixed group were higher than those in the GK nail fixed group (P<0.05). CONCLUSIONS: The design of AMLN accords well with the plastic fixation theory. The generation and conduct of the intermittent physical stress between the fractural parts could resolve the balance problem between stress conduct and stress protection. The fracture end could heal and remodeling at the same time which speed up the fractural healing process.  相似文献   

Th〖ZK(〗e effect of L-phenylalanine on the plasma NO,ET levels and SOD activity in spontaneous hypertensive rats     

CHE Zai-qian  FAN Chun-ling  GU Tian-hua  WU Yong-jie  ZHAO Guang-sheng 《园艺学报》2007,23(2):389-390

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细辛挥发油抗植物病原真菌活性初步研究   总被引：12，自引：0，他引：12  

刘海燕  高微微  樊瑛  陈士林 《植物病理学报》2007,37(1):95-98

从天然产物包括植物中开发新的高效低毒环境友好型杀虫杀菌剂，是农药领域重要的研究内容。细辛（Asarum）为马兜铃科（Aristolochiaceae）草本植物，是我国特有的传统中药，中医用于治疗风寒头痛、痰饮咳喘、关节疼痛、鼻塞、牙痛、胃炎以及口腔糜烂等。有文献报道，细辛提取物对黄曲霉（Aspergillus sp．）、黑曲霉（Aspergillusr sp．）、人类致病菌白色念珠菌（Candida sp．）、伤寒杆菌（Salmonella sp．）有抑菌作用；对人参黑斑病菌（Alternaria panax）、人参根疫病菌（Phytophthora cactorum）、腐皮镰孢（Fusarium solani）和番茄灰霉病菌（Botrytis cinerea）等植物病原菌具有较好的抗菌效果。有关细辛抗菌机制的研究尚未见报道。本文选择9属16种植物病原真菌进行生物测定，探讨细辛挥发油抗菌的广谱性，并以Alternaria humicola为研究对象，对其抗菌机理进行初步研究，以便为开发新型植物杀菌剂提供理论依据。  相似文献   

脂肪型脂肪酸结合蛋白的生理功能及在禽类中的研究进展     

范芮铭  孟博  曹中赞  栾新红 《动物医学进展》2017,38(5)

脂肪型脂肪酸结合蛋白(A-FABP)又称为脂肪酸结合蛋白4(FABP4)或A-FABP,是脂肪酸结合蛋白(FABPs)家族中的一员,能结合多种疏水性化合物,在脂肪和卵巢组织中分布广泛,参与脂肪酸的合成代谢,作用于卵巢组织中,对于禽类产蛋中脂肪酸的沉积会起到关键的作用,调控禽类产蛋过程中蛋的脂肪酸营养沉积,提高产蛋优良性能。该蛋白又与肌间脂肪(IMF)含量有关,保证了肉质的良好品质,这让AFABP在禽类养殖中的研究逐渐成为热点。论文就该蛋白的生理功能和在禽类中的研究进展做一介绍。  相似文献   

獭兔被毛检测仪的研制与开发初报     

陈芸莹  范康  范成强  陈琳 《草业与畜牧》2013,(2)

以现代光电仪器及计算机科学和技术原理与方法,开发将实物描述为图像,建立数学模型转换为数据读出结果的软件；研制取样系统、显像系统、识别系统、显示系统、传导系统,并进行便携、实用、美观的部件加工、组装、调试,成功研制出了獭兔皮毛长度、直径、密度快速检测仪.试用结果精准度优于切片检测,为獭兔和毛皮动物毛皮质量的检测提供了先进的仪器和检测技术.  相似文献   

Dynamic monitoring of biomass and vegetation coverage in rodent damaged grassland regions of Qinghai Province,China     

CHEN Meng-die  HUANG Xiao-dong  HOU Xiu-min  FENG Qi-sheng  YU Hui  GUO Zheng-gang  LIANG Tian-gang 《草业学报》2013,22(4):247

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