湖南省长沙市羊布鲁氏菌病基线调查     

刘增再  谭丹  薛爽  陈智  唐爱明  赖诗嘉  邓钰  宁华杰 《中国动物检疫》2018,35(3):6-9

为了解湖南省长沙市布鲁氏菌病流行现状,对奶羊养殖场(户)和种羊场采用普查策略,对其他羊养殖场(户)采用两阶抽样策略,选择羊群并选定群体中个体,采集血样进行布鲁氏菌抗体检测。同时,通过问卷调查和查阅资料等方式,分析布鲁氏菌病感染和传播风险。结果显示:全市羊布鲁氏菌病表观场群流行率为1.54%(95%CI:0.42%~3.91%),表观个体流行率为0.60%(95%CI:0.43%~0.80%);4个阳性群集中分布于长沙县和宁乡县;和以往监测数据相比,长沙市的羊群布鲁氏菌病流行率和人间发病率均呈下降趋势,说明前期的防治策略取得积极成效。总体来说,长沙市布鲁氏菌病流行率较低,疫情处于稳定控制状态,因而可以分区域逐步实现全市布鲁氏菌病净化。  相似文献   

慈竹生长过程纤维素、木质素及灰分动态积累     

冯芳敏  胡尚连  周振华  曹颖  卢学琴  徐刚  龙志坚  任鹏 《福建林业科技》2015,(1):7-10,25

以当年生不同生长高度的慈竹为研究对象,探讨慈竹生长过程中纤维素、木质素和灰分含量的积累规律。结果表明:慈竹不同生长高度纤维素含量顶、中部变化趋势均以对数方式增长;基部纤维素含量和茎秆纤维素总含量变化趋势都呈"S型"曲线;各部位木质素含量变化趋势总体呈倒"S型"曲线,且不同高度间的木质素含量的差异达显著水平;随着生长高度的增高,茎秆顶部、基部灰分含量以及茎秆灰分总含量总体呈倒"S型"曲线变化,中部灰分含量总体呈"S型"曲线变化。纤维素总含量、木质素总含量的积累分别集中在茎秆高度为150~800、30~800 cm这个生长时期;灰分总量在300~800 cm保持较高的水平。  相似文献   

Identification of Suspected Stem/progenitor Cells in Bovine Mammary Epithelial Cells Culture System     

HAO Yu-meng  ZHAO Feng  ZHANG Na  CHEN Dan  LI Qing-zhang 《中国畜牧兽医》2016,43(8):2095-2103

To develop the potential function of dairy cow mammary stem cells (DCMECs) in regulation of lactation,we identify putative DCMECs which were BrdU label retaining epithelial cells,at the same time,analysis the location of two new mammary stem cells molecular marks FNDC3B and PROCR to verify the feasibility of them to indicate DCMECs.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-1 and their receptors were detected along with cell passage by Real-time quantitative PCR.The results showed that the proportion of BrdU label-retaining epithelial cells was nearly 0.4% after 25 d continuous culture (passaged 4 times) and few cells were positive for FNDC3B or PROCR.Moreover,we observed the BrdU labelled epithelial cells by asymmetric division.The mRNA levels of prolactin,growth hormone,insulin-like growth factor-Ⅰ and their receptors in primary and passage cells were extremely significant difference(P<0.01).DCMECs would rapidly lose some physiological characteristics and the ability of milk synthesis when not under the condition of induction of lactation differentiation,but a certain percentage of mammary stem/progenitor cells will be retained,whose potential effects on the regulation of lactation and mammary acinar remodeling were worthy of attention.  相似文献   

贵州息烽天台山黄心夜合种群结构和动态研究初报   总被引：1，自引：0，他引：1  

冯祥麟  胡刚  刘正华  张百军 《种子》2011,30(7)

在以黄心夜合(Michelia martinii)为优势种群的森林群落内设置1200 m2样地,用相邻格子法进行每木调查获取野外资料.通过对种群大小级(立木级)数量特征和分布格局分析,探讨了黄心夜合种群结构和不同发育阶段种群格局动态.结果表明,黄心夜合种群结构呈不完整的金字塔型,中小径级种群缺失较多,但有一定数量的幼苗幼树(Ⅰ级和Ⅱ级),仍属于稳定型种群或进入成熟型阶段.种群不同发育阶段分布格局由集群分布向随机分布转变,集群强度由强变弱,种群密度由大变小,群落仍处于相对稳定阶段.  相似文献   

Identification of Differentially Expressed Genes and Lipid Metabolism Signaling Pathways between Muscle and Fat Tissues in Broiler Chickens     

Min Zhang  Dan Zheng  Zhimei Peng  Yuting Zhu  Rongrong Li  Qiong Wu  Yan Li  Hongyi Li  Weihua Xu  Mao Zhang  Qichun Hung  Yanfa Sun 《The Journal of Poultry Science》2021,58(2):131

In this study, signaling pathways and key differentially expressed genes (DEGs) involved in lipid metabolism in muscle and fat tissues were investigated. Muscle and abdominal fat tissues were obtained from 35-day-old female broilers for RNA sequencing. DEGs between muscle and fat tissues were identified. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of DEGs were performed. A total of 6130 DEGs were identified to be significantly enriched in 365 GO terms, most of which were involved in biological processes, cellular components, and molecular functions in muscle and fat tissues. Three important lipid signaling pathways (pyruvate metabolism, the insulin signaling pathway, and the adipocytokine signaling pathway) were identified among the fat and muscle tissues of broilers. The key common DEGs in these pathways included phosphoenolpyruvate carboxykinase 2 (PCK2), acetyl-CoA carboxylase 1 alpha and beta (ACACA and ACACB), and the mitogen-activated protein kinase (AMPK) gene family. Hence, our findings revealed the pathways and key genes and gene families involved in the regulation of fat deposition in the muscle and fat tissues of broilers.  相似文献   

狗尾草应用基础的研究进展     

吴子龙  王磊  张浩  王晓改  李丹  赵昕 《北方园艺》2021,(5):130-135

狗尾草具有NADP-ME型C4光合作用,适合高光效形成机制的研究,生命周期短暂,基因组较小,植株低矮,种子数量多,便于进行遗传操作,被认为是研究C4光合作用的模式物种,并且狗尾草生长要求简单,抗旱耐盐,适应性广,与谷子、柳枝稷近缘,又是理想的旱生禾本科粮食作物和生物能源植物的遗传研究模型.该研究介绍了狗尾草的研究基础,综述了狗尾草的生长发育特性、生理生化特性、种质资源、功能基因以及药用价值的研究进展,并对狗尾草的发展趋势进行展望.  相似文献   

生物质液化油分级产物的分离和综合利用研究     

冯君锋  蒋剑春  徐俊明  贺小亮  杨中志  许乐 《林产化学与工业》2014,(6)

以浓硫酸为催化剂,采用甲醇-甘油复合溶剂,在高温高压的条件下对竹屑、杨木、桉木和松木进行液化,并且对液化产物进行了中和、过滤和旋转蒸发等一系列分级处理,并对液化固体残渣和气体产物进行分析。主要研究了以竹屑为原料的液化油的分级产物的组成及成分,研究结果表明:竹屑经甲醇-甘油液化,产物经过分级处理得到的主要产物为杂多酚、多糖苷和乙酰丙酸甲酯。多糖苷相主要含有多糖苷和未反应的甘油及其甘油聚合物,其中多糖苷主要为六元环吡喃糖苷,约占41.81%,甘油及其聚合物占47.27%;杂多酚相主要含有乙酰丙酸酯、杂多酚和甘油等化合物,其中甘油及其聚合物占38.04%,杂多酚相物质为难溶于水的焦油相的相对分子质量大的物质,约占杂多酚相的38.41%,并且杂多酚相的相对分子质量主要集中在990左右。  相似文献   

两种南海海绵放线菌的分离和培养研究     

欧阳永长  梁梓添  姚雅琪  冯颖  王鹏飞 《湖北农业科学》2014,(13):3002-3005

利用3种培养基对两种南海海绵Axinyssa和Halichondria共附生的放线菌(Actinobacterial)进行分离和培养,共得到41个放线菌菌株,分别属于Brachybacterium、Janibacter、Dermacoccus、Brevibacterium、Saccharomonospora、Arthrobacter、Micromonospora、Tsukamurella和Streptomyces 9个属的14个种,其中Y12和Y13为候选新种属。这些放线菌能抑制金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌的生长,其分子方法显示这些放线菌具有产生聚酮类化合物和非核糖体多肽的潜力。  相似文献   

Wood modification with furfuryl alcohol catalysed by a new composite acidic catalyst     

Wanju Li  Hankun Wang  Dan Ren  YunShui Yu  Yan Yu 《Wood Science and Technology》2015,49(4):845-856

  相似文献   

人参皂苷Rb1对微血管自律性舒缩活动及大鼠心肌微血管内皮细胞内钙瞬时变化的影响     

王鑫  冯波  孙向婉  李平丽  董虹  穆祥 《畜牧兽医学报》2015,(1):156-162

探讨人参皂苷Rb1(GSRb1)对人合谷穴区皮内微血管自律性舒缩活动振幅及大鼠心肌微血管内皮细胞(RMMECs)内游离钙离子瞬时变化的影响。利用激光多普勒血流测定仪结合离子导入仪检测微血管自律性舒缩活动的振幅。利用激光共聚焦显微镜、钙离子荧光探针Fluo-3AM检测ATP引起的RMMECs细胞内钙离子的变化及Rb1对其的影响。结果表明:试验确定了能够引起RMMECs细胞内游离钙离子释放的ATP的最低量为0.5μg·mL-1,瞬时升高差异显著的剂量是0.7μg·mL-1,能够影响RMMECs细胞内钙离子变化的GSRb1的最低量为8μg·mL-1。RMMECs经8μg·mL-1 GSRb1孵育后,能够刺激细胞内游离钙离子释放的ATP的最低量升高为1.5μg·mL-1。本研究说明,提高微血管自律运动振幅的中药成分GSRb1能够通过对RMMECs胞内钙离子的影响,提高RMMECs对ATP的耐受能力(ATP的刺激量由0.5μg·mL-1升高到1.5μg·mL-1)。  相似文献