Detection of nitrogen cycle genes in soils for measuring the effects of changes in land use and management     

M.J. Colloff  S.A. Wakelin  D. Gomez  S.L. Rogers 《Soil biology & biochemistry》2008,40(7):1637-1645

Nutrient cycles represent key links between above- and below-ground ecosystems. But obscurity of functional diversity of nutrient-cycling organisms has constrained our understanding. We studied the microbial ecology of the nitrogen cycle using bacterial genes involved in nitrification (amoA), denitrification (napA) and nitrogen fixation (nifH) from areas with highly contrasting soils, climates and land management practices within Australia. Agricultural soils had greater frequency of occurrence of nitrogen cycling genes compared with areas of adjacent remnant native vegetation. The relative abundance of the nitrate reductase gene, napA, increased following the addition of urea to soils, indicating a pulse-response of nitrate-reducing bacteria. Laboratory perturbation of soils with highly saline water had no effect on the frequency of functional gene detection, but following perturbation of soils with antibiotics, the presence of amoA fell below detectable levels while levels of ammonia increased over time. In contrast, nifH and napA were detected in some soils for the duration of the perturbation experiments. This observed positive relationship between gene abundance and respective process rates was indicative of an active bacterial population. As such, detection and quantification of bacterial genes involved in the nitrogen cycle represents a model system for gaining insights to soil microbial diversity and ecosystem function.  相似文献   

A pilot study to investigate the measurement of immunoglobulin A in Welsh Cob and Welsh Pony foals’ faeces and their dam’s milk     

CB Riley  CJ Jenvey  FJ Baker  A Corripio 《New Zealand veterinary journal》2020,68(4):225-230

ABSTRACT

Aims: To determine if an ELISA for measurement of IgA in equine serum could be used to measure concentrations of IgA in foal faeces and to determine correlations with concentrations in the milk of the dam.

Methods: Faeces from 20 Welsh Cob and Welsh Pony foals and milk from their dams were collected within 12?hours (Day 0) and at 6 days after parturition (Day 6). On Day 6, faeces could not be collected from 2/20 foals, and milk samples could not be collected from 3/20 mares. An equine IgA ELISA validated for serum and plasma was used to measure concentrations of IgA in all samples in triplicate. The precision of the assay for each sample type was determined using modified CV.

Results: IgA was not detectable in 7/20 Day 0 faecal samples and in 2/18 Day 6 faecal samples. For samples with detectable IgA, the mean modified CV was 10.5 (95% CI?=?6.0–15.0)% for Day 0 faecal samples, and was 6.8 (95% CI?=?4.3–9.4)% for Day 6 faecal samples. Median concentrations of IgA in faeces on Day 0 were lower than concentrations on Day 6 (0.7?mg/g vs. 37?mg/g dry matter; p?=?0.003). Concentrations of IgA in milk and faeces on Day 6 were statistically correlated (r?=?0.59; p?=?0.006).

Conclusions and clinical relevance: The IgA ELISA showed acceptable precision when used to estimate concentrations of IgA in foal faeces during the first week of life, but IgA could not be detected in 37% of meconium samples collected on Day 0. This assay may be useful for investigation of the role of maternal milk IgA in the gastrointestinal tract of neonatal foals, but further assessment of both accuracy and precision of the ELISA is required.  相似文献   

Evaluation of a new strategy to modulate skeletal development in racehorses by imposing track-based exercise during growth: the effects on 2- and 3-year-old racing careers   总被引：2，自引：0，他引：2  

Rogers CW  Firth EC  McIlwraith CW  Barneveld A  Goodship AE  Kawcak CE  Smith RK  van Weeren PR 《Equine veterinary journal》2008,40(2):119-127

REASON FOR PERFORMING STUDY: No data exist on the effects of conditioning exercise at foal age on workload and subsequent clinical injury rate during their 2- and 3-year-old racing careers. OBJECTIVES: To investigate the effects of subjecting TB foals to conditioning exercise prior to the start of race training on: the workload required to reach a level of fitness sufficient to compete; and the prevalence of orthopaedic injury during the first 2 seasons of their racing careers. METHODS: Twenty 18-month-old TBs, 12 subjected to conditioning exercise at foal age (CONDEX) and 8 exercised spontaneously at pasture only (PASTEX) were trained and entered in competitive events. Workload was quantified using the cumulative workload index (the product of average velocity and distance at a specific gait) and the animals were monitored clinically and radiographically for signs of musculoskeletal disorders. RESULTS: Workload to reach the desired fitness level was similar for CONDEX and PASTEX. CONDEX performed more prerace training sessions as 2-year-olds (P<0.05). The incidence of orthopaedic injuries was low in both groups and there were no differences in the occurrence of orthopaedic ailments. PASTEX animals tended to show signs of musculoskeletal disorders earlier than CONDEX animals. This time difference was significant for metacarpophalangeal joint pain on flexion, reduced carpal flexion and hindlimb lameness (P<0.05). CONCLUSIONS: Subjecting TB foals to conditioning exercise early in life does not have adverse effects on racing careers at ages 2 and 3 years, and does not influence the workload needed to reach a fitness level that is sufficient for racing. POTENTIAL RELEVANCE: The lack of negative effects and the indications of some positive effects of early conditioning exercise in the Thoroughbred encourage further large-scale comparative studies.  相似文献   

Unexplained underperformance syndrome in sport horses: classification, potential causes and recognition     

Rivero JL  van Breda E  Rogers CW  Lindner A  van Oldruitenborgh-Oosterbaan MM 《Equine veterinary journal》2008,40(6):611-618

When inadequate training stress is applied and recovery time is insufficient, performance reduction and chronic maladaptation occurs. Known as overtraining syndrome (OTS), this complex condition afflicts horses in top training. The name of the syndrome implies causation and it is necessary to differentiate it from over-reaching, a term used in horses that, after suffering a loss of performance without an obvious clinical reason, recover their performance within 1 or 2 weeks. The term OTS should be used for horses in heavy training losing performance without an obvious clinical reason and which is sustained for > 2 weeks. Despite considerable scientific investigations, the underlying mechanisms are still poorly understood and there are no objective biomarkers for OTS. An imbalance between training and recovery seems to be the primary cause of this dysfunction. However, other factors such as transport, feeding, subclinical disease and general management may play a role. There is now sufficient evidence that red cell hypervolaemia is not a mechanism for the OTS in horses. A dysfunction of the hypothalamo-pituitary-adrenocortical axis is momentarily the only mechanism that may be related to the development of this syndrome. In the absence of a scoring system to assess the psychological status of horses, the most effective method for diagnosis is the thorough assessment of the history and presenting complaint (length of unexplained performance deficit, weight loss despite adequate feed intake, concurrence of unspecific subclinical problems, unsuccessful treatments and changes of behaviour). Standardised exercise tests are suggested to provide a way to detect subtle changes in hormonal responses in the individual, which may make an important contribution to the detection of early overtraining. But further longitudinal studies are needed to clarify mechanism(s) underlying OTS and to establish the potential for the use of a physiological test as a predictive tool of this disorder.  相似文献   

Evaluation of a new strategy to modulate skeletal development in Thoroughbred performance horses by imposing track-based exercise during growth   总被引：2，自引：0，他引：2  

Rogers CW  Firth EC  McIlwraith CW  Barneveld A  Goodship AE  Kawcak CE  Smith RK  van Weeren PR 《Equine veterinary journal》2008,40(2):111-118

REASON FOR PERFORMING STUDY: No data exist on the intensity of exercise required or on possible harmful effects of increasing exercise in foals over the natural level when free at pasture. OBJECTIVES: To investigate whether an increase in workload over free pasture exercise in the period from directly after birth to the start of training is tolerated by Thoroughbred (TB) foals without increasing injury rate or producing other undesired side effects. METHODS: Thirty-three TB foals were allocated to one of 2 exercise groups directly after birth. One group (PASTEX) was raised on pasture and the other (CONDEX) kept under identical circumstances, but was additionally subjected to an exercise protocol of gradually increasing intensity. Foals were monitored periodically and scored for the presence of clinical signs related to the musculoskeletal system (joint effusion, pain at flexion, occurrence of physeal swelling), and radiographs taken at the end of the conditioning phase. Also, behavioural studies were performed to detect any changes in behaviour related to the exercise programme. Cortisol levels were measured in both groups, to assess the level of stress. RESULTS: Workload in the CONDEX group was significantly higher than in the PASTEX group (approximately 30%). Conditioning increased the likelihood for joint effusion in the antebrachiocarpal joint, but reduced tarsocrural effusion and physeal swelling at the lateral distal radius, the third metacarpal bone (medial aspect) and lateral and medial aspects of the third metatarsal bone. CONCLUSIONS: The 30% increase in workload did not affect the animals' welfare, effects of conditioning exercise on clinical musculoskeletal health were few and there were no adverse effects. POTENTIAL RELEVANCE: This study supports the feasibility of imposing early conditioning exercise in horses and is a benchmark for its effects on the development of equine musculoskeletal tissues.  相似文献   

Risk factors for time to diagnosis of feline upper respiratory tract disease in UK animal adoption shelters     

Edwards DS  Coyne K  Dawson S  Gaskell RM  Henley WE  Rogers K  Wood JL 《Preventive veterinary medicine》2008,87(3-4):327-339

Feline upper respiratory tract disease (URTD), mainly caused by feline calicivirus (FCV) and feline herpesvirus, is a major cause of disease outbreaks in feline accommodation such as animal shelters, catteries and multi-cat households. We conducted a longitudinal, yearlong study in five UK feline animal shelters to identify risk factors for the time to diagnosis of URTD. We were especially interested in risk factors that could be identified at the time the cat entered the shelter. Shelter staff recorded data for 1434 cats during 2002–2003. Most of the cats were domestic shorthair cats and were from private households, or were stray or abandoned. Sixty cats without clinical signs of URTD at entry had URTD diagnosed (typically within the first month at the centre). We used two multivariable models: one was a Cox proportional-hazards model, and the other a regression analyses with complementary log–log model.The hazard varied substantially between shelters and was considerably lower for the shelter that had a purpose-built admissions unit with its own isolation facilities. The hazard was greater for purebred cats (HR 4.3–5.0) and for neutered cats (HR 2.0). The hazard was also typically greater if the centre had a greater proportion of cats present with URTD. The analyses suggested that the centre-level risk factors were more important in determining hazard than cat-level risk factors.  相似文献   

Evaluation of butorphanol-azaperone-medetomidine in captive cheetah (Acinonyx jubatus) immobilization     

Aleksandr Semjonov  Jacobus P. Raath  Liesel Laubscher  Toomas Orro  Silke Pfitzer  Toomas Tiirats  Peter Stewart Rogers  Vladimir Andrianov 《Veterinary anaesthesia and analgesia》2019,46(1):90-95

Objective

The butorphanol-azaperone-medetomidine fixed-dose combination (BAM, respectively, 30-12-12 mg mL^?1) with subsequent antagonism by naltrexone-atipamezole was evaluated for reversible immobilization of captive cheetahs (Acinonyx jubatus).

Study design

Prospective, clinical trial.

Animals

Twelve cheetahs (six males and six females, weighing 37–57 kg) housed in enclosures, were immobilized at Hoedspruit Endangered Species Centre in the Republic of South Africa.

Methods

BAM volume dose rate was 0.009–0.014 mL kg^?1 (mean ± standard deviation 0.010 ± 0.001 mL kg^?1). Total dose in all animals was 0.5 mL. The actual doses were as follows: butorphanol (0.29 ± 0.04 mg kg^?1), azaperone (0.12 ± 0.01 mg kg^?1) and medetomidine (0.12 ± 0.01 mg kg^?1). Physiologic variables and quality of immobilization were recorded every 5 minutes beginning at 15–20 minutes after darting. Arterial blood samples were collected three times at 20, 30 and 40 minutes after darting from all animals for analysis of blood oxygenation and acid-base status.

Results

The inductions were calm and smooth and mean induction time was 4.0 ± 1.1 minutes. Heart rate (50 ± 9 beats minute^?1) and respiratory frequency (20 ± 3 breaths minute^?1) were stable throughout immobilization. The recovery time after reversing with naltrexone and atipamezole was 9.1 ± 3.6 minutes.

Conclusions

and clinical relevance BAM proved to be a reliable and cardiovascular stable drug combination for immobilization of cheetahs.  相似文献   

Ultrasonographic evaluation of vitreous degeneration in normal dogs.     

Julien J Labruyère  Claudia Hartley  Katherine Rogers  Graham Wetherill  J Fraser McConnell  Ruth Dennis 《Veterinary radiology & ultrasound》2008,49(2):165-171

Vitreous degeneration is common in dogs and may be associated with cataract formation. Vitreous degeneration may be identified using B-mode ultrasonography and appears as multiple, small, motile, point-like echoes within the vitreous cavity. In humans, vitreous degeneration has also been observed in normal aging eyes but the incidence of vitreous degeneration in dogs without cataract has not previously been documented. The purpose of this study was to describe the ultrasonographic appearance of vitreous degeneration and to investigate its incidence in a population of dogs without cataract or other apparent eye disease. The eyes of 62 dogs were evaluated as part of a prospective study. All dogs underwent ophthalmological and ultrasonographic examinations and vitreal changes were graded on ultrasonography using a predetermined grading scheme. Vitreous degeneration was found in 20% (23/114) of the eyes on ultrasonographic examination but in only 8% (9/114) of eyes on direct ophthalmoscopy. Sensitivity and specificity of ophthalmoscopy using ultrasonography as a gold standard were respectively, 39% and 100%. Vitreal syneresis and asteroid hyalosis could be distinguished according to their ultrasonographic characteristics. The probability of having vitreous degeneration increased with the age of the dog (odds ratio = 6.7 for dogs of 7 + years compared with 0-6 years) and also increased in females compared with males (odds ratio = 3.6). Vitreous degeneration, especially mild vitreal syneresis, is not uncommon in normal dogs; it was shown to be an age-related condition and its significance should not be overinterpreted on ocular ultrasonography.  相似文献   

Effects of exercise on chondrocyte viability and subchondral bone sclerosis in the distal third metacarpal and metatarsal bones of young horses     

Dykgraaf S  Firth EC  Rogers CW  Kawcak CE 《Veterinary journal (London, England : 1997)》2008,178(1):53-61

The objective was to determine the effects of early exercise on the articular cartilage and subchondral bone at specific sites of the distal third metacarpal and metatarsal bones of 12 young Thoroughbred horses allowed free choice exercise at pasture. Six of the horses had additional controlled exercise 5 days per week from mean age of 21+/-20 days of age (range: 3-83 days) until 17.1 months of age. Confocal laser scanning microscopy was used to quantify viable and non-viable chondrocytes. Proteoglycan scoring and modified Mankin scoring was performed and subchondral bone mineral density measured by computed tomography. The number of viable chondrocytes was significantly greater in the conditioned group, which also had a higher Safranin O/Fast Green (SOFG) score than did the group which could exercise only at pasture. There was no difference in mean bone mineral density between groups, nor was there relationship between subchondral bone mineral density and chondrocyte viability. The apparent beneficial effects of early conditioning exercise may support the use of exercise to optimise development of articular cartilage in young individuals.  相似文献   

Growth curves from birth to weaning for Thoroughbred foals raised on pasture     

Morel PC  Bokor A  Rogers CW  Firth EC 《New Zealand veterinary journal》2007,55(6):319-325

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