HPLC法测定兔血清中盐酸沙拉沙星含量的研究     

李银生  侗恒敏  李艳华  刘远飞  丛霞   《西南大学学报(自然科学版)》2000,22(3):219-221

建立了ＨＰＬＣ（高效液相色谱）法测定兔血清中盐酸沙拉沙星的含量。固定相：色谱柱,ＨｙｐｅｒｓｉｌＣ１８柱,１０μｍ,Φ４．６＊２００ｍｍ。保护柱,ＨｙｐｅｒｓｉｌＣ１８柱,１０μｍ,Φ４．６＊３０ｍｍ。均由大连依利特科学仪器有限公司购进。流动相：０．０１％三氟乙酸－乙腈 －３％四丁基溴化铵（６０：２５：１５ｖ／ｖ／ｖ）流速：１．０ｍｌ／ｍｉｎ。保留时间３．２－３．８ｍｉｎ。灾光检测器检测波长：激发  相似文献   

机械化播种不同坐水量对玉米出苗率的影响   总被引：5，自引：0，他引：5  

裴泽莲  丛福滋  王洪平  张旭东  李宝筏 《沈阳农业大学学报》2005,36(4):508-510

坐水播种试验采用2BQMS-2型坐水免耕气吸播种施肥机进行的,该机一次作业完成开沟、施水、施肥、播种、覆土等多道工序,用鼠道暗式施水开沟器,暗灌水,水肥同施,上层覆盖干土,以减少水分散发。通过不同施水量对出苗率、长势及产量进行田间试验,测试数据经方差及多重分析,结果表明,在土壤含水率在8%时,45m3.hm-2坐水量对出苗率、长势及产量是最经济有效的方案,且节水效果显著。  相似文献   

基于GIS技术的荔城平原区耕地土壤重金属污染程度及其风险评价   总被引：2，自引：0，他引：2  

丛艳静  邢世和  罗文光 《福建农业学报》2007,22(3):302-307

在空间插值基础上,利用Hakanson潜在生态风险指数法,研究了荔城平原耕地土壤重金属的污染程度及其潜在生态风险。结果表明,研究区8种重金属中汞污染指数最高,综合污染指数均值为0.897,处于警戒线水平,但总体上较清洁;在重金属潜在生态危害指数中,汞危害系数较高,达到47.496,处于中度危害水平,其余重金属元素的潜在危害系数为0.362~12.058,均处于轻度危害水平;综合潜在风险指数介于27.742~127.344,均值为68.129,处于轻度危害;并利用GIS软件绘制出污染风险评价分区图。  相似文献   

玉米秸杆覆盖下小麦免耕播种机的试验研究     

丛祥安  刘凤波  杨晓波 《辽宁农业职业技术学院学报》2006,8(4):22-23

在分析小麦免耕播种机作业性能要求的基础上,研制在玉米秸杆覆盖下小麦免耕播种的结构特征、工作原理,以带状粉碎防堵装置为核心的免耕播种机,通过粉碎轴粉碎玉米秸杆和田间杂草,有效地解决了开沟器缠草堵草的问题。  相似文献   

黄土丘陵区不同年限柠条林地土壤质量变化   总被引：1，自引：0，他引：1  

张晋爱张兴昌  邱丽萍丛伟闫峰  薛晓辉 《农业环境科学学报》2007,26(B03):136-140

在野外调查和室内分析的基础上,探讨了0、10、22、26和30 a时间序列柠条林地土壤物理、化学及酶活性的动态变化.结果表明柠条林可提高土壤粘粒、粉粒含量,10～26 a土壤容重显著降低;除全磷表现为减少趋势外,随生长年限延长,柠条林地有机质、全氮、硝态氮、铵态氮、速效磷和速效钾含量均呈现增加趋势;土壤蔗糖酶、碱性磷酸酶、脲酶和过氧化氢酶活性随柠条生长年限增加呈增加趋势,但各种酶表现最佳状态的时间不同,且蔗糖酶和碱性磷酸酶对柠条林地土壤变化的敏感性高于脲酶和过氧化氢酶.  相似文献   

沧临管道泄漏实时监测系统的设计与应用     

吴宏斌  从德胜  张厚亮 《油气储运》2004,23(3):56-58

沧临管道泄漏实时监测定位系统综合吸收了多种先进的检测技术和信号处理方法,采用检测泄漏点瞬态负压波的方式实现管道的泄漏检测和定位.通过介绍沧临管道泄漏实时监测系统的工作原理、系统组成、软件设计和应用实例,表明该系统对各监测数据的实时采集、传输和处理的速度较快,具有较强的数据网络监控功能和较高的管道泄漏检测灵敏度和定位精度,可以有效地保障输油管道的安全运行.  相似文献   

英文广告中的修辞艺术     

吕方源 《信阳农业高等专科学校学报》2007,17(3):159-160

修辞可以增强语言的趣味性和生动性,在英文广告中具有不可低估的作用。英文广告中常用的修辞手法有:音韵(包括头韵和尾韵)、排比、夸张、双关、仿拟等。  相似文献   

小鼠肝炎病毒RT-PCR检测方法的研究     

赖国旗  何明忠  谭毅  王胜  潘永全  韦克  何伏秋  蒋虹  佟巍  丛喆 《西南大学学报(自然科学版)》2004,26(6):762-763,772

用4株小鼠肝炎病毒(MHV1,MHV3,A59,JHM)分别感染DBT细胞,收获病毒,提取病毒RNA。根据病毒基因的特异性保守序列设计引物,在最佳务件下进行RT—PCR,建立了RT—PCR检测方法,即两步法和一步法,分别扩增出600bp,375bp的特异性核酸片段,其中,一步法检测MHV更快速、简便。  相似文献   

Sequence Analysis of mtDNA COIGene and Molecular Phylogeny of Different Geographical Populations of Bemisia tabaci (Gennadius)     

CHU Dong  ZHANG You-jun  CONG Bin  XU Bao-yun  WU Qing-Jun  ZHU Guo-Ren 《中国农业科学(英文版)》2005,4(7)

Bemisia tabaci(Gennadius) is a serious pest in many cropping systems worldwide and occurs in different biotypes. The mtDNA COI gene of the 12 Bemisia tabaci (Gennadius) populations from different regions and countries were analyzed.Based on mtDNA COI sequences, their biotypes were characterized and phylogenetic relationships among these populations were established with the method of UPGMA. The results indicated the genetic similarity between those populations from Beijing, Zhengzhou, Zaozhuang, Nanjing, Shanghai, Haikou, and the B-biotype populations from California, Texas, Arizona reached 99.8-100%, which meant the nation-wide infested populations of B. tabaci in China in recent years were B-biotypes. Another population collected from Kunming of Yunnan Province showed very high similarity with Q-biotype B. tabaci from Spain and Morocco, which meant the Kunming population was Q-biotype. This is the first report on the invasion of Q-biotype into China.  相似文献   

Prostaglandin E₂ receptors,EP2 and EP4, regulate expression of surface molecules and cytokines in B-cells of collagen-induced arthritic mice     

ZHANG Jing-ge  CHEN Hai-ying  QIN Jin  CONG Bin  LI Qiao-xia  JIA Xian-xian  MA Chun-ling  YU Feng 《园艺学报》2011,27(3):545-549

AIM: To observe the immunoregulatory effects of prostaglandin E₂ receptor (EP) subtypes EP2/EP4 on the B-cells of collagen-induced arthritic(CIA)mice. METHODS: DBA/1 mice were immunized with chicken type II collagen emulsified in Freunds complete adjuvant to induce arthritis. B-cells were isolated from the splenocyte suspension by positive selection using anti-CD19 monoclonal antibody immunomagnetic beads. The expression of MHC II, CD 80 and CD86 was examined by flow cytometry. The mRNA levels of EPs, interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), IL-6, IL-4, IL-10 and transforming growth factor-β (TGF-β) were detected by real-time RT-PCR. RESULTS: The rank of the mRNA levels of EPs was EP2>EP1>EP3>EP4 in B-cells and EP2/EP4 mRNA expression was obviously increased in CIA mice. EP2 antagonists inhibited the expression of MHC II, CD80 and CD86. EP4 antagonist had little effect on CD80. EP2/EP4 antagonists inhibited the mRNA expression of IFN-γ, TNF-α, and IL-6 (P<0.05 or P<0.01) and increased the expression of IL-10 (P<0.01 or P<0.05). Furthermore, the antagonists of EP2 and EP4 also increased the mRNA expression of IL-4 and TGF-β (P<0.01), respectively. CONCLUSION: PGE₂ modulate the pathogenesis of CIA via EP2/EP4 by regulating the expression of surface molecules and cytokines in B-cells. EP2/EP4 may be a new therapeutic target for treating rheumatoid arthritis.  相似文献