In vitro production of porcine zygotes using intracytoplasmic injection of vitrified sperm          下载免费PDF全文

CC Arraztoa  C Baca Castex  GM Alvarez  PD Cetica  DM Neild 《Reproduction in domestic animals》2017,52(5):775-780

The objectives of this study were to evaluate if vitrified porcine spermatozoa are able to maintain their capacity to produce zygotes in vitro using intracytoplasmic sperm injection (ICSI) and to evaluate the zygote development in two in vitro atmospheric conditions: 5% CO₂ and tri‐gas. A group of porcine oocytes maturated in vitro were injected with vitrified‐warmed sperm (treatment group) and another group, with sperm diluted and conserved at 17°C (control group). To evidence parthenogenetic activation, some oocytes were submitted to a Sham test. The injected oocytes were cultured in G1 medium at 38°C, 100% humidity and 5% CO₂ or tri‐gas. No significant differences (p > .05) were observed in embryo development between the oocytes injected with vitrified‐warmed sperm (31.8%; 36/113), and those injected with semen diluted and conserved at 17°C (35.5%; 32/90), when cultured in 5% CO₂ or under tri‐gas atmosphere (42.9%; 39/91 vs. 34.2%; 26/76, respectively). No significant differences (p > .05) were observed in the percentage of pronuclei (PN) obtained between 5% CO₂ and tri‐gas, within each treatment either. Of the 52 oocytes submitted to the Sham test, only two presented a female PN (activation) indicating that the PN observed in the treatment group were a product of fertilization and not parthenogenetic activation. To conclude, porcine sperm vitrified using spheres, at a concentration of 5 × 10⁶ spermatozoa/ml in TALP medium with 1% bovine serum albumin (BSA), conserve condensed and intact chromatin capable of producing early embryo development up to the pronuclear stage.  相似文献   

Potato Response to Phosphorus Fertilization at Two Sites in Nova Scotia,Canada     

Judith Nyiraneza  Keith D. Fuller  Aimé J. Messiga  Benoît Bizimungu  Sherry Fillmore  Yefang Jiang 《American Journal of Potato Research》2017,94(4):357-366

Identifying phosphorus (P) requirements and P-use efficiency is crucial to a sustainable potato industry in Eastern Canada. In a three-year study (2013–2015) conducted in Nova Scotia, Canada, we assessed the potato (CV Superior) yield response to P fertilization on two different soils in the Annapolis Valley. Soil organic matter ranged from 3.0% to 4.1% and from 2.0% to 2.5% at the Kentville and Sheffield sites, respectively. Initial soil phosphorus was higher at Kentville site, ranging from 81 to 162 mg kg^?1 than at Sheffield site ranging from 75 to 109 mg kg^-1. A randomized complete block design was used with five P rates (0, 17.5, 35, 52, and 79 kg P ha^?1) applied at planting. Tuber yields were assessed at harvest, and P-uptake efficiency characteristics were measured before vine senescence. Total and marketable yields were not impacted by P rates. Marketable yield was 68% and 57% greater for Kentville than for Sheffield in 2013 and 2015, respectively and were significantly affected by P rates × year interactions at a 5.4% probability level. Quadratic functions were used to describe tuber yield responses to P rates (0.61 > R ² < 0.85) and P rates corresponding to the maximum yields were 17.5 kg P ha^?1 in 2013 and 2015 and 35 kg P ha^?1 in 2014 when data from both sites were pooled together. Phosphorus uptake efficiency ranged from 0.47 to 0.54 g DM mg^?1 P offtake at Kentville and from 0.45 to 0.49 g DM mg^?1 P offtake at Sheffield and was 13% and 7% greater at Kentville than at Sheffield in 2013 and 2015, respectively. While further studies are still needed for recalibration, results from this study provide some of the first information regarding potato response to P fertilization in Nova Scotia. Based on current P recommendations in the region for the same soil P levels, our results suggest that current P rates can be reduced without impacting potato yield.  相似文献   

Application of a real‐time polymerase chain reaction assay to the diagnosis of bovine ephemeral fever during an outbreak in New South Wales and northern Victoria in 2009–10     

DS Finlaison  AJ Read  J Zhang  R Paskin  PD Kirkland 《Australian veterinary journal》2014,92(1-2):24-27

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A cross-sectional pilot study to estimate the prevalence of and risk factors for leptospirosis in South-Western Victorian dairy herds, 2017     

E Erregger  MA Stevenson  DS Beggs  S Oswin  SP Jagoe  PD Mansell  MF Pyman 《Australian veterinary journal》2020,98(9):417-423

Leptospirosis is a zoonosis, found worldwide, affecting many species of animals. We conducted a cross-sectional study to estimate the prevalence of Leptospira borgpetersenii sv Hardjo and Leptospira interrogans sv Pomona in cattle in dairy herds in South-Western Victoria, Australia. Fifty-three herds were enrolled in the study. Urine samples were collected from 15 late-lactation cows in each herd. A questionnaire was provided to herd managers at the time of each herd visit, asking them to describe the methods they used for controlling leptospirosis, including vaccination. Urine samples were pooled at the herd level and tested for leptospira spp. using real time PCR. Urine samples from individual cows within the positive pooled samples were then tested for Leptospira Hardjo and Leptospira Pomona using qPCR. Four of the 53 herds showed positive leptospirosis results giving an apparent prevalence of 8 (95% CI 2–18) leptospira-positive herds per 100 herds at risk. Based on the 53 completed questionnaires, leptospirosis vaccination programs were not compliant with label directions in 36 of the 52 vaccinated herds: 69 (95% CI 55–81) of 100 herd managers that routinely vaccinated for leptospirosis did not comply with label directions. One herd was completely unvaccinated. Based on our findings, we estimate that approximately 10% of dairy farms in South-Western Victoria are likely to be infected with leptospirosis. While most herds are vaccinating for leptospirosis, most are not doing so according to label directions. We conclude that herd managers need to be better educated regarding leptospirosis vaccination programs.  相似文献   

Laparoscopic resection of an exostosis of the os pubis in a horse     

Mario Delcazo  Florian Geburek PD  Dr med vet  DECVS  Kernt Köhler Dr med vet  Michael Röcken Dr med vet  Felix Theiss Dr med vet  PhD  DECVS 《Veterinary surgery : VS》2020,49(3):614-620

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Encephalomyocarditis virus infection in alpacas     

TW O'Connor  DS Finlaison  LK Manning  MS Hazelton  ZB Spiers  P Pinczowski  EM Bolin  PD Kemsley  NU Horadagoda  AJ Dart  RW Cook  PD Kirkland 《Australian veterinary journal》2020,98(10):486-490

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ULTRASONOGRAPHIC ASSESSMENT OF METALLIC CLIP CAROTID ANASTOMOSIS IN SHEEP     

Marco A.  Lima  DVM  Carmen  Calles  DVM  Juan R.  Lima  DVM  Maria F.  Martin  PD PhD  Veronica  Crisostomo  DVM  Francisco J.  Hernandez  MD PhD  Rafael  Barrera  DVM PhD  Jesus  Uson  DVM PhD 《Veterinary radiology & ultrasound》2002,43(2):171-177

The aim of this study was to use ultrasonography to assess an experimental carotid arteriotomy followed by end-to-end anastomosis in the lamb. Animals were subjected to an experimental surgical procedure consisting of a right carotid artery arteriotomy and subsequent end-to-end anastomosis with nonpenetrating metallic clips. Left side arteries were used as control. Duplex ultrasonography was performed postoperatively to assess the evolution of the experimental anastomosis. Measurements were made on the right carotid to assess blood flow, peak systolic velocity, and end diastolic velocity, as well as arterial diameter. Animals were periodically checked during the six-month postoperative period. Operated vessels appeared normal during follow up, with pulsatile movements and size similar to non operated carotid arteries. A hyperechoic band, perpendicular to the vessel main axis, was seen on the longitudinal image in operated arteries, but not in the control group. This band corresponded to the anastomosis area, where the clips were positioned. No image suggestive of thrombosis was observed in any operated artery, and blood flow was maintained through the experiment. Finally, a decrease of vessel lumen diameter was evidenced at the level of the anastomosis during the first 30 postoperative days, but it was not present after the 90th postsurgical day. Duplex ultrasonography is a useful imaging technique for the assessment of anatomical details, acoustical properties of the vascular lumen, and study of the intimal surface and vessel wall in growing arteries subjected to surgery. Metallic clips as suturing technique in arterial anastomosis allow for normal vascular growth, as shown by the evolution of flow velocity and vessel diameter.  相似文献   

The role of veterinarians on animal experimentation ethics committees     

LR SCOTT  PD CARTER 《Australian veterinary journal》1996,74(4):309-311

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Management of Italian and Perennial Ryegrasses for Seed and Forage Production in Crop Rotations     

H. T. Kunelius  K. B. McRae  G. H. Dürr  S. A. E. Fillmore 《Journal of Agronomy and Crop Science》2004,190(2):130-137

Italian ryegrass (Lolium multiflorum Lam.) and perennial ryegrass (L. perenne L.) can be grown for seed and forage in cold winter regions provided the stand persists well over winter. Seed yield and plant characteristics during primary growth, and forage yield during regrowth, were determined for two Italian and one perennial ryegrass cultivars in Atlantic Canada. Establishment methods and dates included sowing ryegrass in cultivated soil alone or with barley in mid‐May and, after harvesting the barley crop, by sowing ryegrass following conventional or reduced cultivation and by no‐till drilling into barley stubble in mid‐August and early September. Despite some winterkill, particularly in Italian ryegrass, seed and forage yields were adequate in post‐establishment growing seasons. Seed yield for Italian ryegrass was greatest (1270 kg ha^?1) when it was sown into cultivated soil in mid‐August and least (890 kg ha^?1) when sown alone in May. Italian ryegrass yielded 15–17 % more seed when plots were established in mid‐August rather than in mid‐May or early September. Italian ryegrass cv. Lemtal had a greater density of fertile tillers (1030 m^?2) in the sward than cv. Ajax (860 m^?2) and its tiller density was greater when seeded into cultivated soil in September than in mid‐August. There were fewer spikelets per seed head for sowing Italian ryegrass with barley in May than for the other methods of establishment. Forage yield in regrowth was greater for Italian ryegrass cv. Ajax (2770 kg ha^?1) than for cv. Lemtal (2480 kg ha^?1). Seed yield of perennial ryegrass was greater when seeded in mid‐May than in mid‐August or early September. The seed yield of perennial ryegrass was greater when it was sown with barley in May and harvested for grain, than when it was sown alone or with barley harvested at late milk stage. The establishment methods for mid‐August and early September sowing had little effect on seed yield. However, the no‐till and reduced tillage methods resulted in a greater tiller density than sowing into the cultivated seedbed. Fertile tillers tended to be denser under reduced cultivation for sowing in August. Forage yield of perennial ryegrass regrowth was not influenced by the sowing method and timing. In conclusion, Italian and perennial ryegrasses produce adequate seed and forage regrowth under different establishment methods and timing. However, the poor persistence of Italian ryegrass may limit commercial production after the establishment year in Atlantic Canada.  相似文献   

Effect of body position on electrocardiographic recordings in dogs     

JA Stern  KW Hinchcliff  PD Constable 《Australian veterinary journal》2013,91(7):281-286

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