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51.
The spatial and temporal distribution of spawning activity by autumn‐run Chinook salmon Oncorhynchus tshawytscha (Walbaum) was examined across multiple years. The study period included two years of extreme drought conditions when water temperatures in the spawning reach of the Stanislaus River were considered sub‐optimal for spawning and egg incubation. Despite varying levels of superimposition, redd counts and densities remained stable and positively associated with river location, indicating that superimposition may be driven by habitat preference rather than the absence of suitable spawning locations. Spawning occurred slightly later during drought years (6–10 days later compared with wetter years). This delay was attributable to deferred migration instead of deferred spawning, and the majority of redds were constructed at water temperatures exceeding the optimal temperature range. As a consequence, estimates of juvenile production during 2014 and 2015 were among the lowest on record. These findings may be related in part to the high hatchery contribution to the population, above‐average temperatures during spawning and incubation, and superimposition rates. Management recommendations include adequate cold‐water storage in the upstream reservoir, refined spawning habitat restoration techniques in the light of superimposition rates and, on a broader system scale, actions that reduce the amount of stray hatchery Chinook salmon.  相似文献   
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Objective   To determine a reference range for serum cobalamin concentration in healthy cats in Australia using a chemiluminescent enzyme immunoassay and to prospectively investigate the prevalence of hypocobalaminaemia in cats with non-alimentary tract disease.
Design   Prospective study measuring serum cobalamin concentrations in clinically healthy cats and cats with non-alimentary tract illness.
Procedure   Blood was collected from 50 clinically healthy cats that were owned by staff and associates of Veterinary Specialist Services or were owned animals presented to Creek Road Cat Clinic for routine vaccination. Blood was collected from 47 cats with non-alimentary tract illness presented at either clinic. Serum cobalamin concentration was determined for each group using a chemiluminescent enzyme immunoassay.
Results   A reference range for Australian cats calculated using the central 95th percentile in the 50 clinically healthy cats was 345 to 3668 pg/mL. Median serum cobalamin concentration in 47 cats with non-alimentary tract illness (1186 pg/mL; range 117–3480) was not significantly different to the median serum cobalamin of the 50 healthy cats (1213 pg/mL, range 311–3688). Using the calculated reference range one sick cat with non-alimentary tract illness had a markedly low serum cobalamin concentration.
Conclusion   Although hypocobalaminaemia is uncommon in sick cats with non-alimentary tract illness in Australia, its occurrence in this study warrants further investigation.  相似文献   
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Objective   To identify and gain an understanding of the influenza viruses circulating in wild birds in Australia.
Design   A total of 16,303 swabs and 3782 blood samples were collected and analysed for avian influenza (AI) viruses from 16,420 wild birds in Australia between July 2005 and June 2007. Anseriformes and Charadriiformes were primarily targeted.
Procedures   Cloacal, oropharyngeal and faecal (environmental) swabs were tested using polymerase chain reaction (PCR) for the AI type A matrix gene. Positive samples underwent virus culture and subtyping. Serum samples were analysed using a blocking enzyme-linked immunosorbent assay for influenza A virus nucleoprotein.
Results   No highly pathogenic AI viruses were identified. However, 164 PCR tests were positive for the AI type A matrix gene, 46 of which were identified to subtype. A total of five viruses were isolated, three of which had a corresponding positive PCR and subtype identification (H3N8, H4N6, H7N6). Low pathogenic AI H5 and/or H7 was present in wild birds in New South Wales, Tasmania, Victoria and Western Australia. Antibodies to influenza A were also detected in 15.0% of the birds sampled.
Conclusions   Although low pathogenic AI virus subtypes are currently circulating in Australia, their prevalence is low (1.0% positive PCR). Surveillance activities for AI in wild birds should be continued to provide further epidemiological information about circulating viruses and to identify any changes in subtype prevalence.  相似文献   
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Streptococcus suis is an important swine pathogen and a zoonotic agent. Differences in virulence have been noted among the 33 described serotypes, serotype 2 being considered the most virulent. In this study, we aimed at assessing the serotype distribution and the production of virulence-associated markers by strains recovered from diseased pigs in the United States (U.S.). Results showed that among the 100 strains evaluated, serotype 3 (20% of the isolates) and serotype 2 (17%) were the most prevalent. We then investigated the presence in these isolates of the genes sly, epf and mrp, encoding the virulence-associated markers suilysin (SLY), extracellular factor (EF) and muramidase-released (MRP) protein, respectively. The effective production of the markers by the strains was also verified. Results showed that the presence of the gene did not always correlate with actual expression of the respective protein. In the case of MRP, this was due, in most cases, to frameshift mutations at the 5′ end of the gene resulting in premature stop codons. The most prevalent phenotypes among U.S. strains were MRP+EFSLY (40%) and MRPEFSLY+ (35%). Serotype distribution greatly differed from that reported in several European countries, as did the production of virulence markers, particularly for serotype 2. On the other hand, our results for the U.S. S. suis isolates are similar to those reported for Canadian strains, suggesting a common status in North America.  相似文献   
56.
We tagged fingerling white bass, Morone chrysops, with passive integrated transponders (PIT) at two body locations (peritoneal cavity and dorsal musculature) and six weight classes to evaluate survival, tag retention, and weight gain during a 28-day experimental period. The probability of survival (as calculated by logistic regression) was >95% for fingerlings >19 g for both PC and DM. Retention of PIT tags varied between PC and DM tagged white bass in 6 g and 14 g fingerlings. The probability of tag retention was 100% in all sizes of fingerlings tagged PC, and >95% for fish >19 g tagged DM.  相似文献   
57.
Hybrid striped bass fingerlings were grown in replicated indoor tanks for 42 days to evaluate progeny performance of two growth-related traits. Analysis of variance demonstrated significant differences in length and weight of fingerlings among paternal and maternal half-sib families and high estimates of heritability for dams and sires. Based on significant sire and dam effects and high heritabilities, a substantial portion of the selection differential could be expected to be gained in offspring from selected pure-line parents. As demonstrated by these results, a breeding program could be used to select parental lines that produce faster growing hybrid offspring.  相似文献   
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The scale of damage caused by vertebrates to the plastic wrap on baled silage was evaluated on Irish farms. A survey was conducted on 300 farms representative of farming systems and geographical locations throughout Ireland. The scale of damage was classified as farms with 0%, 1-10%, 11-50% or 51-100% of bales with holes in the plastic wrap caused by birds. The proportion of farms within these four classes with damage caused by birds during the short interval between wrapping and removal from the field to storage was 47%, 37%, 15% and 1% of the total, respectively. Correspondingly, during the subsequent storage prior to feedout, bird damage was 37%, 50%, 12%, and 1%. Similarly, the proportions for damage throughout long-term storage due to cats were 71%, 23%, 6% and 0%, while the incidence of damage caused by rats, dogs and farm livestock was comparatively small. The stored bales were fenced against livestock on 86% of farms, while netting, bale painting and other protection methods were used to discourage damage on 2%, 17% and 9% of farms, respectively. The damage caused by birds was more prevalent on farms where the bales were stored on their curved side rather than their flat end, more than twotiers high and in farmyards rather than in fields. Cats damaged the sides and shoulders of the peripheral bales within bale collections, particularly where wrapped bales were stored in the farmyard.  相似文献   
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