Amplification,Sequence Analysis and Tissue Expression Detection of FUT2 Gene Coding Regions of Dongchuan Pigs     

QIN Wei-yun  GAN Li-na  ZHAO Cheng-xiang  YU Li-huai  BAO Wen-bin  WU Sheng-long 《中国畜牧兽医》2017,44(1):1-11

Porcine alpha (1,2) fucosyltransferase (FUT2) gene was importance in glycosphingolipid biosynthesis-globo series, potentially played a regulatory role during Escherichia coli (E. coli) F18 infection process in weaned piglets. In order to explore sequence structure of porcine FUT2 gene and its biological function, this test amplified FUT2 gene CDS sequence of Dongchuan pigs by PCR, forecasted and analyzed the protein sequences and functional regions of FUT2 gene, its expression level was detected in 11 tissues of 8 Dongchuan weaned piglets in 35 days old at the meantime. The results showed that the CDS sequence of FUT2 gene was 1 023 bp, which encoded 340 amino acids. FUT2 protein was fat-soluble hydrophilic protein, which the structure was not stable, including a transmembrane helix structure, but without signal peptide that suggested the FUT2 protein was a membrane protein;FUT2 protein included 2 N-glycosylation sites (No. 185 and No. 305 amino acids), without O-glycosylation sites, there were 14 potential phosphorylation sites, included 6 Ser, 2 Thr and 6 Tyr, analyzing the functional regions found that the FUT2 protein had a superfamily of conserved domains:FUT1-FUT2-like (58-319 amino acids). The phylogenetic tree result showed that the relative relationship between swine and cattle was relatively close, but was distant from chimpanzee, human, mouse and rat. FUT2 gene was expressed in all 11 tissues of Dongchuan weaned piglets, there were higher expression in digestive tract and immune tissues. The present results suggested that FUT2 gene might play a role to resistance to E. coli F18 in weaned piglets, and might indirectly against E. coli F18 through the synthesis of fucosyltransferase.  相似文献   

1FFSL-5型浅翻深松翻转犁的设计与试验     

甘露  孙大明  程亨曼 《农机化研究》2008,(5):136-138

保护性耕作技术及配套机具已成为当今世界农业发展的主题，深松技术作为保护性耕作的一项关键技术在我国已得到广泛应用。为此，进行了浅翻深松翻转犁的总体设计，确定了工作原理和主要参数，并通过试验进行了验证。结果表明：该样机达到了设计指标，具有一定的应用价值。  相似文献   

锰肥对孜然芹产量的影响   总被引：2，自引：2，他引：0  

高杰  刘立强  高翔  甘付华 《新疆农业大学学报》2003,26(4):74-75

通过对孜然芹种子使用0．05％．0．10％，0．50％，1．00％4个浓度的MnSO4进行浸种处理，结果表明，用0．05％和0．10％的MnSO4溶液浸种增产效果显著，增产幅度分别为46％和28％，而0．5％和1．0％的处理均无增产效果。  相似文献   

国内外汽车市场需求分析     

杨沿平  甘年妃  刘克进 《湖南农业大学学报(自然科学版)》2003,(5)

根据大量调查统计数据和汽车市场变化规律 ,分析目前国内外 (尤其是国内 )汽车市场情况和发展趋势 ,并对 2 0 10年国内汽车市场主要产品和技术需求进行了预测。  相似文献   

构建基于ArcGIS的林业工程信息系统   总被引：1，自引：0，他引：1  

甘澍 《江西林业科技》2008,(2):62-64

从提高林业工程数据管理的效率、效果和效益出发,探讨构建基于ArcGIS的县级林业工程信息系统的方法和步骤,以期为建立多层级互联共享的林业工程信息系统储备基础数据和相关技术。  相似文献   

长叶榧叶部油挥发性成分的色谱/质谱分析研究   总被引：1，自引：0，他引：1  

吴渝  杨湄  李培武  寿建尧  胡乐华  张文  陈洪  甘冬生 《中国油料作物学报》2004,26(3):97-99

采用气相色谱/质谱(GC-MS)联用法测定了长叶榧叶部精油的挥发性成分,初步发现其主要成分是柠檬烯、反式α-蒎烯、β-蒎烯、顺式α-蒎烯、3-莰烯、6,6-二甲基-2-亚甲基-环庚烷、β-月桂烯,其含量分别为32.2%、12.5%、7.1%、6.6%、6.6%、5.5%、4.4%.  相似文献   

都市农业与城郊农业的内在联系与转变接点     

干劲天 《北京农业职业学院学报》2001,15(4):37-38

都市农业是在城郊农业内部产生和发展起来的。都市农业与城郊农业在其生长地域、功能、以及产业布局等十个方面存在重要的内在联系和转变接点。  相似文献   

狗牙根种质资源及遗传多样性的研究概况   总被引：7，自引：0，他引：7  

张小艾  张新全  杨春华  刘伟  干友民 《草原与草坪》2003,(4):3-6

介绍了国内外狗牙根种质资源的研究概况;从形态学水平、染色体水平、蛋白质水平、DNA水平对狗牙根的遗传多样性进行概述,并对存在的问题提出建议。  相似文献   

基于建构主义学习理论的医学生物化学教学模式探索     

姚裕群甘建华  杨剑萍 《现代农业科学》2008,(2):94-96

对医学生物化学教学的现状进行了分析，剖析了影响学生学习医学生物化学课程的若干因素，尝试在医学生物化学教学中改变传统的“以教师为中心”的教学模式，并从建构主义学习理论及与该理论相适应的建构主义教学模式入手，探索新的医学生物化学教学模式。  相似文献   

Effect of 3 isoforms of Ikaros on proliferation of human ovarian cancer SKOV3 cells     

HE Li-cai  CHEN Shang  ZHU Zhen-feng  GAN Jun  YU Hui-min 《园艺学报》2015,31(8):1407-1411

AIM: To investigate the effect of Ikaros isoforms on the proliferation of human ovarian cancer SKOV3 cells. METHODS: Three isoforms of Ikaros, IK1, IK2 and IK6, were transfected into ovarian cancer SKOV3 cells. CCK-8 assay and cell counting were used to detect the effects of Ikaros isoforms on the proliferation of SKOV3 cells. The cell cycle was analyzed by flow cytometry. The cell cycle-related proteins were detected by Western blot. RESULTS: IK1 and IK2 expression inhibited SKOV3 cells proliferation. Flow cytometry analysis indicated that IK1 and IK2 induced SKOV3 cell cycle arrest at the G₁ phase. IK6 isoform exerted no obvious effect on the proliferation or cell cycle of SKOV3 cells. Compared with control EV group, IK1 group and IK2 group showed a dramatic elevation in the expression of the cell cycle inhibitor p21, along with a substantial decrease in the expression of the cell cycle inducers cyclin D1 and cyclin D2, which did not change in IK6 group. CONCLUSION: IK1 and IK2 significantly inhibit the proliferation of ovarian cancer SKOV3 cells and induce cell cycle arrest at G₁ phase by regulation of cell cycle-related proteins cyclin D1, cyclin D2 and p21, while IK6 isoform exerts no obvious effect on the proliferation and cell cycle of SKOV3 cells.  相似文献