The IMPDH inhibitors,ribavirin and mycophenolic acid,inhibit peste des petits ruminants virus infection     

Qiu-yan Chang  Fu-cheng Guo  Xue-rui Li  Jian-hua Zhou  Xuepeng Cai  Qiuwei Pan  Xiao-xia Ma 《Veterinary research communications》2018,42(4):309-313

Peste des petits ruminants virus (PPRV) causes highly contagious diseases in domestic and particular wild small ruminants, leading to substantial economic loss. The development of effective and cheap antiviral medications shall help to circumvent this emerging burden. In this study, we found that ribavirin, a competitive inosine-5’-monophosphate dehydrogenase (IMPDH) inhibitor, significantly inhibits the replication of PPRV. As IMPDH is a key enzyme in purine nucleotide synthesis, supplementation of exogenous guanosine attenuate the anti-PPRV effect of ribavirin. Interestingly, an uncompetitive IMPDH inhibitor, mycophenolic acid (MPA), exerted more potent antiviral effect again PPRV. Similarly, this effect was largely restored upon supplementation of guanosine. Thus, we have demonstrated that the IMPDH inhibitors ribavirin and MPA combat PPRV infection through purine nucleotide depletion. Because both regimens have been widely used in the clinic for treating viral infection or organ rejection in transplantation patients for decades, respectively, repurposing these existing safe and cheap medications may provide a new avenue for combating PPRV infection.  相似文献   

白藜芦醇和褪黑素对绵羊胎儿成纤维细胞转染及卵母细胞体外成熟的影响     

姚昱君  李广栋  吴昊  马文奎  杨海  关盛宇  吕东颖  付瑶  朱天奇  姬鹏云  谭鑫星  赵万民  连正兴  张鲁  刘国世 《中国畜牧兽医》2021,48(12):4497-4507

试验旨在利用白藜芦醇(Re)和褪黑素(MT)提高绵羊胎儿成纤维细胞电穿孔转染(电转)效率、优化卵母细胞体外成熟体系,以提高转基因动物生产效率。将绵羊胎儿成纤维细胞分为6组:对照组、添加白藜芦醇高、中、低(R^-5、R^-6、R^-7)3种浓度组及白藜芦醇和褪黑素联合添加组(R^-5M^-5、R^-6M^-7),通过电转效率和细胞凋亡情况确定电转体系优化方案;通过对细胞周期、线粒体膜电位及活性氧(ROS)的检测研究其作用机制。将绵羊卵母细胞分为对照组及白藜芦醇和褪黑素(R^-5M^-5、R^-6M^-7)联合添加组,通过绵羊卵母细胞体外成熟后卵丘细胞扩展、第一极体排出及体外受精胚胎的卵裂率检测其对卵母细胞体外成熟的作用。研究结果显示,分离的绵羊胎儿成纤维细胞P3代增殖旺盛,细胞生长曲线呈典型的S型;与对照组相比,所有试验组胎儿成纤维细胞的电转效率均显著提高(P<0.05),R^-5M^-5组电转效率最高;R^-6M^-7组显著降低细胞凋亡率(P<0.05),R^-5、R^-6、R^-5M^-5和R^-6M^-7组均显著促进细胞增殖(P<0.05);R^-6、R^-7、R^-5M^-5和R^-6M^-7组G1期细胞增加,S期细胞相对减少,但差异均不显著(P>0.05);R^-6、R^-7、R^-5M^-5和R^-6M^-7组成纤维细胞内ROS水平均显著降低(P<0.05),R^-6、R^-5M^-5和R^-6M^-7组线粒体膜电位均显著增加(P<0.05);R^-6M^-7组显著促进绵羊卵丘细胞扩展、提高卵母细胞第一极体排出率和体外受精胚胎发育能力(P<0.05)。综上,白藜芦醇和褪黑素可以优化绵羊胎儿成纤维细胞电转体系,提高卵母细胞体外成熟效率,为白藜芦醇和褪黑素在转基因动物生产中的应用提供参考依据。  相似文献   

中国石油海外管道完整性管理的必要性和挑战     

李大全  黄文  汪是洋  管磊  祝宝利  梁江 《油气储运》2015,(1):19-23

针对国家油气战略通道安全平稳运行的需要,基于中国石油海外油气管道的基本特点,分析了海外油气管道实施完整性管理的必要性。根据中国石油海外管道安全运行的技术需求和油气管道完整性管理技术的特征,分析了中国石油海外油气管道实施完整性管理面临的技术挑战。为提高海外管道安全水平,避免海外投资遭受损失,需尽快开展海外管道的完整性技术应用工作。在具体实施过程中,需要与国内管道完整性管理技术研究及应用单位相结合,充分发挥中国石油集团公司整体技术优势。  相似文献   

Effects of ‘Tent’ Mulching on Soil Temperature and Grape Growth in The Yellow River Delta Saline-Alkali in Spring     

WANG Hui  ZHAO Shuo  YANG XingWang  JIN MengLing  DU YuanPeng  GUAN XueQiang  ZHAI Heng 《中国农业科学》2019,52(16):2871-2879

  相似文献   

13个广西喜树种源的喜树碱含量分析     

刘毅  苏付保  粟本超  冯立新  兰健花 《广西林业科学》2017,46(1)

以13个广西喜树(Camptotheca acuminata)种源的嫩叶为试材,研究不同种源对喜树碱含量的影响。结果表明,不同种源的喜树碱含量有极显著差异,不同土壤类型和海拔种源间的喜树碱含量有显著或极显著影响。13个广西喜树种源的喜树碱含量从高到低排序为德保田林金秀隆林德峨兴安西林天峨永福凌云东兰隆林新州南丹灵川。德保、田林、金秀喜树种源是优良种源。  相似文献   

Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig     

QIN Zhao-xian  NONG Su-qun  GUAN Zhi-hui  LIANG Dan  QIN Qian  LAN Xi  PAN Tian-biao  XIE Bing-kun 《中国畜牧兽医》2016,43(9):2223-2229

This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to predicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment included a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48% of similar nucleotide sequence with that of Bos,Pan troglodytes,Homos,Macaca,Rattus,Mus,Gallus and Danio retio,respectively.The protein structure analysis results showed that the protein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 16 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly conserved in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.  相似文献   

星点木组培芽扦插技术研究     

桂明春  管艳  田海  李玲  王亚  梁国平 《黑龙江农业科学》2015,(1)

为了探索星点木的大规模繁殖技术,以星点木四年生植株当年生枝条、半年生组培苗枝条和组培试管芽条为试材,研究了不同激素种类、浓度及不同插穗来源对星点木试管外扦插生根的影响。结果表明:星点木试管外扦插生根率较高,生长激素能够很好地促进生根,50mg·L-1 IBA处理后生根综合指标较好,生根率可达95%以上,根系效果指数达0.549。半年生组培苗枝条较适合作为插穗来源。  相似文献   

高校图书馆基于IC的知识管理与虚拟学习社区融合研究     

袁媛  苏建华 《农业图书情报学刊》2011,23(3):126-129

知识管理与虚拟学习具有相关性与互补性,高校图书馆信息共享空间为知识管理与虚拟学习社区的融合创造了有利条件,因此知识管理与虚拟学习社区的融合是可行的,但是面临来自于观念、技术、功能、内容、交互性等方面的障碍。图书馆应尽可能地采用现代信息技术将知识管理系统与虚拟学习社区集成在一个IC内,进而提升自身的服务水平。  相似文献   

Effect of eleutheroside B/E on proliferation and expression of PPARγ and TGF-β1 in HBZY-1 cells cultured with high glucose     

WU Jian-hua  DU Yin-xiang  HUANG Qiang 《园艺学报》2015,31(3):511-517

AIM: To explore the effects and mechanism of eleutheroside (ETS) B or E on the proliferation of HBZY-1 cells treated with high glucose. METHODS: The HBZY-1 cells were cultured under high glucose condition. The 4th generation of HBZY-1 cells was used for determining the optimal cell density, which was consistent with the growth regulation curve of the cells. The cells were divided into 6 groups: low glucose (LG) group, high glucose (HG) group, high glucose plus ETS-B/E (low dose, medium dose and high dose) groups, and high glucose plus losartan (LTG) group. After all cells were treated with the corresponding drugs at 24 h, 48 h and 72 h, the inhibitory rate of the proliferation was measured, and the expression of TGF-β1 and PPARγ was detected by immunocytochemistry and Western blotting. RESULTS: The best cell density was 2 000 cells/well, which was complied with the basic rules of the cell growth, and high glucose significantly promoted the HBZY-1 cell proliferation. At each time point, the inhibitory effects of ETS-B/E were significantly different between HG group and LTG group on the proliferation of the HBZY-1 cells (P<0.05). The expression of TGF-β1 was significantly inhibited, and the expression of PPARγ was significantly promoted by ETS-B/E (P<0.05). ETS-E showed stronger effect than ETS-B (P<0.05) in a concentration- and time-dependent manner. CONCLUSION: ETS-B/E significantly inhibits the proliferation of HBZY-1 cells under high glucose condition by decreasing TGF-β1 expression and promoting PPARγ expression.  相似文献   

波叶大黄白粉病病原菌的鉴定     

王博儒  刘淑艳  唐淑荣  管观秀 《植物病理学报》2017,47(6):849-854

正波叶大黄Rheum rhabarbarum L.隶属于蓼科Polygonaceae大黄属Rheum,是一种重要的药用植物。白粉病多发生在波叶大黄的叶部,会影响其光合作用进而降低其药用价值。据笔者统计,目前关于蓼白粉菌Erysiphe polygoni DC.在世界范围内的报道有很多~([1~9]),但其中关于蓼科Polygonaceae波叶大黄白粉病病原菌的报道较少,目前仅有1个  相似文献