水产品中粪肠球菌的分离与鉴定     

陈雨欣  石建华 《农产品加工．学刊》2014,(15):46-47

对送检的水产品冷冻多春鱼籽进行副溶血性弧菌检测,经检测样品中未检出副溶血性弧菌,但在科玛嘉弧菌显色培养基中出现紫色菌落,且在多次送检的水产品中经常出现此形态菌落,经VITEK2及生化鉴定,结果为粪肠球菌。  相似文献   

灰霉菌侵染对番茄幼苗活性氧积累及抗氧化酶活性的影响     

何娟  潘光军  官涵杰  陆光磊  陈江艳  须文 《蔬菜》2020,(5):15-20

为进一步分析活性氧和抗氧化防御系统在番茄抗病信号调控机制中的作用,为提高产量和品质提供科学依据,以4叶1心的番茄幼苗为试验材料,比较番茄幼苗受灰霉菌侵染后叶片O_2~-·以及抗氧化酶、内源褪黑素等的变化。结果表明:番茄幼苗接种灰霉菌后,随着时间的延长,幼苗叶片上病斑斑点逐渐增多,灰霉菌含量显著增多,叶色逐渐变浅,植株表现明显的发病症状;灰霉病菌侵染后番茄体内O_2~-·活性爆发,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性下降,过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性上升,褪黑素含量增加;上述变化都是番茄幼苗为了抵御灰霉菌的生物胁迫而发生的应激反应。综上,植物在遭受病原菌等生物胁迫时不能简单地推断SOD、CAT和POD等抗氧化酶活性一定升高,植物抵御病原菌侵染更多地依赖于抗性信号路径中信号物质的激活和积累。  相似文献   

中华鳖暗温室养殖水体理化因子及微生物多样性分析     

常晓  张菊  张艳芹  闫保国  管越强 《河北渔业》2020,(12):1-8

  相似文献   

环境治理中政府间利益博弈与机制设计     

关华  齐卫娜 《湖南农业大学学报(自然科学版)》2015,(1):100-104

在环境治理中，中央政府和地方政府作为“经济人”为争取自身利益最大化展开竞争博弈，效用目标差异是导致其竞争的根本原因。为实现双方互利共赢的帕累托最优，应设计相应的激励机制以实现地方政府从竞争走向合作，实现地方利益与区域利益的统一，经济增长和环境协调一致，促进社会可持续发展。  相似文献   

Analysis on Target Genes with Mutation in microRNA of Blue-shelled Chicken and White Leghorn     

GUAN Yu-yu  LIAO Rong-rong  WANG Zhen  ZHAO Jing  CHEN Zhen-liang  ZHANG Zhe  XIAO Qian  SUN Hao  WANG Qi-shan  ZHANG Xiang-zhe  YANG Chang-suo  PAN Yu-chun 《中国畜牧兽医》2016,43(6):1413-1421

The study was conducted to explore the potential different characters between Blue-shelled chicken and White leghorn.Global genome microRNA was combined the identified microRNA with complementary lab-predicted microRNA.Then the two breed chicken's SNP data got by GGRS were mapped to the microRNA and focused on SNP that deliberately located in mature-microRNA.Bioinformatics method was adopted for target prediction on microRNA which had SNPs.By further gene enrichment analysis,the study found these genes enriched in 22 GO terms,10 KEGG pathways,and 3 IPA important networks.And they enriched in traits which associated with growth,such as mTOR signaling pathways,Wnt signaling pathways,growth hormone receptor networks and insulin-like growth factor Ⅰ receptor networks.And they also enriched in some laying traits,such as oocyte meiotic signaling pathways and progesterone mature oocytes signaling pathways.The methods and the results might provide references for further studies.  相似文献   

阅读辅导服务在现代图书馆建设中的作用     

王崇皓  关朝明  杨红  张丽丽 《农业网络信息》2015,(1):88-90

本文主要阐明了阅读辅导的含义,论述了阅读辅导服务对现代图书馆建设的作用。  相似文献   

灯盏花成分专用型种源评价与鉴定     

李锐  刘冠泽  卢迎春  杨建文  关德军  杨生超 《热带作物学报》2019,40(9):1713-1722

收集和鉴定了云南省和贵州省共46份灯盏花种质资源,筛选出适合生产不同药品的成分专用型种源,为专用型品种选育提供参考。利用HPLC测定了灯盏花种源10种活性成分含量,相关分析阐明各成分含量间的相关性,聚类分析对种质资源进行分类。结果表明：16份灯盏花种源灯盏乙素（scutellarin, SE）含量在2.5%以上,是培育灯盏花素专用型品种的优良材料,其中4份种源同时含有高含量（>1.0%）的双咖啡酰奎宁酸酯（dicaffeoylquinic acids, diCQAs）,是培育灯盏细辛专用型品种的优良材料;同时,筛选出8份总黄酮（total flavonoids, TFs）含量高于3.5%的种源,适合选育总黄酮专用品种。相关性分析表明,SE含量与diCQAs和TFs含量均显著正相关,是灯盏花品质育种的标志性成分。聚类分析将灯盏花种源分为三大类,除1个种源的SE含量和diCQAs含量接近（第Ⅲ类）外,其他种源SE均占总活性成分的60%以上,其中第Ⅰ类为高TFs型种源,TFs占到总活性成分的80%以上,diCQAs占总成分的15%以下;第Ⅱ类为高diCQAs型种源,diCQAs占总成分的16.9%~28.9%,TFs占到总活性成分的80%以下。该结果表明灯盏花种源活性成分组成存在多种类型,为专用型品种选育提供了可能,灯盏乙素含量可以作为专用型种源筛选的首要指标。  相似文献   

Sublytic C5b-9 induces protective autophagy in cultured podocytes     

LV Qian-ying  ZHOU Jian-hua  YANG Feng-jie  PU Jin-yun  ZHANG Yu 《园艺学报》2015,31(1):59-63

AIM: In podocytes, autophagy occurs at a high basal level and dysregulated autophagy is associa-ted with a variety of podocytopathies. This paper is to investigate the role of autophagy in sublytic C5b-9-induced podocyte injury. METHODS: Sublytic complement C5b-9 stimulation was used as an in vitro model. Autophagosomes were confirmed using monodansylcadaverine (MDC) staining. Immunoblotting was used to measure the change of autophagy-related markers. Cellular morphological changes were observed by Wright-Giemsa staining. Immunofluorescence staining and confocal microscopy were used to detect the expression and distribution of nephrin. The cell viability was assessed by methylthiazol tetrazolium (MTT) assay. The cell apoptosis was assessed by Annexin V-fluorescein isothiocyanate/PI staining. RESULTS: For ensuring sublytic complement injury, the maximal amounts of anti-podocyte antiserum and 160×-diluted normal human serum were used without inducing cell lysis (defined as >5% LDH release). Sublytic C5b-9 promoted autophagy of podocytes in vitro. The proautophagic effect of sublytic C5b-9 manifested in the form of accumulated MDC-labeled vesicles and enhanced the expression of LC3-Ⅱ. Autophagy inhibitor 3-methyladenosine (3-MA) promoted sublytic C5b-9-induced podocyte morphological abnormalities. Compared with the sublytic C5b-9-injured podocytes, 3-MA exposure further decreased the expression of nephrin. 3-MA enhanced sublytic C5b-9-induced podocyte apoptosis. CONCLUSION: Sublytic C5b-9 attack induces autophagy, which may play a protective role against complement-mediated podocyte injury.  相似文献   

元谋县冬春蔬菜产业发展面临的竞争态势分析农业CI研究理论体系构建及其实证研究（二）     

彭靖里  李国保  张建华 《农业图书情报学刊》2011,23(7):21-25

结合我国农业特色产业发展实际,在对迈克尔.波特提出的产业分析理论框架改进的基础上,构建了农业情报分析模型,并应用该模型对元谋县冬春蔬菜产业发展中面临的竞争态势进行竞争情报分析,指出其存在的主要问题和原因。  相似文献   

丹参对奶牛脂肪变性肝细胞NF-κB和CYP450表达的影响     

路丽芹  孙立云  徐丽娜  汲如芬  李鹏飞  秦建华  钟秀会  马玉忠 《中国兽医学报》2015,(2):278-282

为探讨丹参对脂肪变性奶牛肝细胞的作用机制,采用胶原酶两步灌流法分离培养奶牛肝细胞,不同浓度DL-乙硫氨酸处理,油红染色观察其脂滴数量,MTT法测定肝细胞的活性,生化法检测TG含量。用不同浓度的丹参处理变性肝细胞后,生化法检测ALT、AST、TG、SOD、MDA的含量或活性,ELISA法测定TNF-α的含量,Western blot法检测NF-κB和CYP450的蛋白表达水平。结果显示,筛选出2 mmol/L的DL-乙硫氨酸作用24 h作为体外造模的最佳条件。用丹参处理后,能降低ALT、AST、TG和MDA的活性或含量,抑制TNF-α的释放,增加SOD活性。随丹参浓度增加和处理时间的延长,CYP450的表达呈逐渐降低的趋势。24 h时,丹参对NF-κB的表达无明显影响;48 h时,随丹参浓度的增加,NF-κB的表达呈逐渐降低的趋势。这表明丹参对奶牛脂肪肝有一定的治疗作用,其作用机制与促进脂质代谢、抑制肿瘤细胞坏死因子、抗脂质过氧化及降低NF-κB和CYP450的表达密切相关。  相似文献