Effects of ‘Tent’ Mulching on Soil Temperature and Grape Growth in The Yellow River Delta Saline-Alkali in Spring     

WANG Hui  ZHAO Shuo  YANG XingWang  JIN MengLing  DU YuanPeng  GUAN XueQiang  ZHAI Heng 《中国农业科学》2019,52(16):2871-2879

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Cloning and Sequence Analysis of PTTG1 Gene in Luchuan Pig     

QIN Zhao-xian  NONG Su-qun  GUAN Zhi-hui  LIANG Dan  QIN Qian  LAN Xi  PAN Tian-biao  XIE Bing-kun 《中国畜牧兽医》2016,43(9):2223-2229

This experiment was aimed to clone PTTG1 gene CDS sequence of Luchuan pig,and was analyzed by bioinformatics methods.A pair of special primers was designed according to predicted sequence of porcine PTTG1 in GenBank.The coding sequence of PTTG1 in Luchuan pig was amplified by RT-PCR,its gene sequence characteristics and protein structure was systemically analyzed by bioinformatics techniques.The results showed that the cloned PTTG1 fragment included a 609 bp CDS (coding 202 amino acids).The sequence multi-aligned results showed that Luchuan pig shared 90.15%,87.85%,87.52%,87.03%,76.03%,74.38%,55.74% and 44.48% of similar nucleotide sequence with that of Bos,Pan troglodytes,Homos,Macaca,Rattus,Mus,Gallus and Danio retio,respectively.The protein structure analysis results showed that the protein attributed hydrophilic protein without signal peptide,localized in cell cytoplasm and had 16 phosphorylation sites.The phylogentic tree of amino acid indicated that PTTG1 was highly conserved in the process of evolution of different species.The cloning and analysis of PTTG1 gene provided an important foundation for further study biological function of porcine PTTG1 during early embryonic development.  相似文献   

星点木组培芽扦插技术研究     

桂明春  管艳  田海  李玲  王亚  梁国平 《黑龙江农业科学》2015,(1)

为了探索星点木的大规模繁殖技术,以星点木四年生植株当年生枝条、半年生组培苗枝条和组培试管芽条为试材,研究了不同激素种类、浓度及不同插穗来源对星点木试管外扦插生根的影响。结果表明:星点木试管外扦插生根率较高,生长激素能够很好地促进生根,50mg·L-1 IBA处理后生根综合指标较好,生根率可达95%以上,根系效果指数达0.549。半年生组培苗枝条较适合作为插穗来源。  相似文献   

波叶大黄白粉病病原菌的鉴定     

王博儒  刘淑艳  唐淑荣  管观秀 《植物病理学报》2017,47(6):849-854

正波叶大黄Rheum rhabarbarum L.隶属于蓼科Polygonaceae大黄属Rheum,是一种重要的药用植物。白粉病多发生在波叶大黄的叶部,会影响其光合作用进而降低其药用价值。据笔者统计,目前关于蓼白粉菌Erysiphe polygoni DC.在世界范围内的报道有很多~([1~9]),但其中关于蓼科Polygonaceae波叶大黄白粉病病原菌的报道较少,目前仅有1个  相似文献   

灰霉菌侵染对番茄幼苗活性氧积累及抗氧化酶活性的影响     

何娟  潘光军  官涵杰  陆光磊  陈江艳  须文 《蔬菜》2020,(5):15-20

为进一步分析活性氧和抗氧化防御系统在番茄抗病信号调控机制中的作用,为提高产量和品质提供科学依据,以4叶1心的番茄幼苗为试验材料,比较番茄幼苗受灰霉菌侵染后叶片O_2~-·以及抗氧化酶、内源褪黑素等的变化。结果表明:番茄幼苗接种灰霉菌后,随着时间的延长,幼苗叶片上病斑斑点逐渐增多,灰霉菌含量显著增多,叶色逐渐变浅,植株表现明显的发病症状;灰霉病菌侵染后番茄体内O_2~-·活性爆发,超氧化物歧化酶(SOD)和过氧化物酶(POD)活性下降,过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性上升,褪黑素含量增加;上述变化都是番茄幼苗为了抵御灰霉菌的生物胁迫而发生的应激反应。综上,植物在遭受病原菌等生物胁迫时不能简单地推断SOD、CAT和POD等抗氧化酶活性一定升高,植物抵御病原菌侵染更多地依赖于抗性信号路径中信号物质的激活和积累。  相似文献   

中华鳖暗温室养殖水体理化因子及微生物多样性分析     

常晓  张菊  张艳芹  闫保国  管越强 《河北渔业》2020,(12):1-8

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环境治理中政府间利益博弈与机制设计     

关华  齐卫娜 《湖南农业大学学报(自然科学版)》2015,(1):100-104

在环境治理中，中央政府和地方政府作为“经济人”为争取自身利益最大化展开竞争博弈，效用目标差异是导致其竞争的根本原因。为实现双方互利共赢的帕累托最优，应设计相应的激励机制以实现地方政府从竞争走向合作，实现地方利益与区域利益的统一，经济增长和环境协调一致，促进社会可持续发展。  相似文献   

Analysis on Target Genes with Mutation in microRNA of Blue-shelled Chicken and White Leghorn     

GUAN Yu-yu  LIAO Rong-rong  WANG Zhen  ZHAO Jing  CHEN Zhen-liang  ZHANG Zhe  XIAO Qian  SUN Hao  WANG Qi-shan  ZHANG Xiang-zhe  YANG Chang-suo  PAN Yu-chun 《中国畜牧兽医》2016,43(6):1413-1421

The study was conducted to explore the potential different characters between Blue-shelled chicken and White leghorn.Global genome microRNA was combined the identified microRNA with complementary lab-predicted microRNA.Then the two breed chicken's SNP data got by GGRS were mapped to the microRNA and focused on SNP that deliberately located in mature-microRNA.Bioinformatics method was adopted for target prediction on microRNA which had SNPs.By further gene enrichment analysis,the study found these genes enriched in 22 GO terms,10 KEGG pathways,and 3 IPA important networks.And they enriched in traits which associated with growth,such as mTOR signaling pathways,Wnt signaling pathways,growth hormone receptor networks and insulin-like growth factor Ⅰ receptor networks.And they also enriched in some laying traits,such as oocyte meiotic signaling pathways and progesterone mature oocytes signaling pathways.The methods and the results might provide references for further studies.  相似文献   

阅读辅导服务在现代图书馆建设中的作用     

王崇皓  关朝明  杨红  张丽丽 《农业网络信息》2015,(1):88-90

本文主要阐明了阅读辅导的含义,论述了阅读辅导服务对现代图书馆建设的作用。  相似文献   

灯盏花成分专用型种源评价与鉴定     

李锐  刘冠泽  卢迎春  杨建文  关德军  杨生超 《热带作物学报》2019,40(9):1713-1722

收集和鉴定了云南省和贵州省共46份灯盏花种质资源,筛选出适合生产不同药品的成分专用型种源,为专用型品种选育提供参考。利用HPLC测定了灯盏花种源10种活性成分含量,相关分析阐明各成分含量间的相关性,聚类分析对种质资源进行分类。结果表明：16份灯盏花种源灯盏乙素（scutellarin, SE）含量在2.5%以上,是培育灯盏花素专用型品种的优良材料,其中4份种源同时含有高含量（>1.0%）的双咖啡酰奎宁酸酯（dicaffeoylquinic acids, diCQAs）,是培育灯盏细辛专用型品种的优良材料;同时,筛选出8份总黄酮（total flavonoids, TFs）含量高于3.5%的种源,适合选育总黄酮专用品种。相关性分析表明,SE含量与diCQAs和TFs含量均显著正相关,是灯盏花品质育种的标志性成分。聚类分析将灯盏花种源分为三大类,除1个种源的SE含量和diCQAs含量接近（第Ⅲ类）外,其他种源SE均占总活性成分的60%以上,其中第Ⅰ类为高TFs型种源,TFs占到总活性成分的80%以上,diCQAs占总成分的15%以下;第Ⅱ类为高diCQAs型种源,diCQAs占总成分的16.9%~28.9%,TFs占到总活性成分的80%以下。该结果表明灯盏花种源活性成分组成存在多种类型,为专用型品种选育提供了可能,灯盏乙素含量可以作为专用型种源筛选的首要指标。  相似文献