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Evaluation of a new immunocapture test for the diagnosis of ovine brucellosis caused by Brucella melitensis 总被引:3,自引:0,他引:3
Durán-Ferrer M Mendoza J Osuna A Caporale V Lucas A León L Garrido F 《The Veterinary record》2002,151(21):629-635
A new immunocapture technique has been applied to the diagnosis of ovine brucellosis under experimental conditions. The tests were made on a serum bank derived from both young and adult ewes vaccinated conjunctivally with the Rev 1 strain at a dose of 10(8) to 10(9) colony-forming units. Adult ewes were infected experimentally two-and-a-half years after they had been vaccinated and the results were compared with an unvaccinated control group. The condition of each animal in terms of infection with Brucella melitensis was determined by clinical and bacteriological investigations. The development of the immune response was compared by the rose bengal test, the complement fixation test, the Coombs' test and the immunocapture technique for 180 days after the vaccination and for 410 days after the experimental infection, that is, the two following gestations. The results suggest that the new technique is more specific in animals vaccinated conjunctivally, regardless of their age when they were vaccinated. After the experimental infection, significantly (P < 0.05) fewer of the vaccinated sheep which were free of clinical signs and were not excreting B melitensis reacted positively to the test. 相似文献
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Martínez Vidal JL Egea González FJ Garrido Frenich A Martínez Galera M Aguilera PA López Carrique E 《Pest management science》2002,58(8):784-790
Principal component analysis (PCA) was applied to the gas chromatographic data obtained from 23 different greenhouse trials. This was used to establish which factors, including application technique (very small, small, medium and large drop-size), crop characteristics (short/tall, thin/dense) and pattern application of the operator (walking towards or away from the treated area) are relevant to the dermal exposure levels of greenhouse applicators. The results showed that the highest exposure by pesticides during field applications in greenhouses, in the climatic conditions and in the crop conditions typical of a southern European country, occurs on the lower legs and front thighs of the applicators. Similar results were obtained by hierarchical cluster analysis (HCA). Drop-size seems to be very important in determining total exposure, while height and density of crops have little influence on total exposure under the conditions of the present study. No pesticide type is a major factor in total exposure. The application of multiple regression analysis (MRA) allowed assessment of the relationships between the pesticide exposure of the less affected parts of the body with the most affected parts. 相似文献
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百合LfMADS基因植物表达载体的构建及其功能分析 总被引:3,自引:0,他引:3
为了分析百合LfMADS1和LfMADS3基因的功能,分别将其正、反义基因插入到植物组成型双元载体pBin438中,并通过根癌农杆菌LBA4404介导转化烟草。PCR和PCR-Southern杂交结果均证明外源基因已经插入到烟草基因组中。转LfMASD1反义基因植株中1朵花的雄蕊极短、花药缺失;转LfMASD1正义基因植株中发现1个花萼变瓣的突变体。在转LfMASD3反义基因植株中发现1个植株的苞叶部分瓣化,花柄变短,另外1个植株上发现1朵花缺失1枚雄蕊;而转LfMASD3正义基因植株中没有发现变异。作者认为LfMASD1是百合花器官发育的B功能基因,LfMASD3是百合花器官发育的SEP基因,这些基因在烟草中的表现说明百合的花器官特性基因的表达模式与模式植物有所不同。 相似文献
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农业是人类的衣食之源,生存之本,是国家向前发展的基石。因此,从多角度分析研究农业发展水平、空间分异,对区域农业可持续发展具有重要的现实意义。以宁夏回族自治区为例,从人口子系统、经济子系统、社会子系统、资源环境子系统等4个维度构建宁夏农业可持续发展评价指标体系,采用熵值法、综合加权求和模型、三维趋势分析、探索性空间数据分析(ESDAGIS)方法,研究了2010—2014年宁夏农业可持续发展。结果表明:总体上宁夏农业可持续发展水平处于波动上升的趋势,南北方向上递增,东西方向呈"倒U型"分布;农业可持续发展表现出空间自相关模式,主要集中在"高高型"和"低低型"象限,其中"高高型"主要分布在银川市、永宁县、平罗县等北部引黄灌区,"低低型"主要集中在南部山区和中部干旱带,根据不同的区域农业可持续发展状况提出相应的对策建议。 相似文献
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随着我国对外经济技术交流的日益扩大,将有越来越多的农业科技人员走出国门、走向非洲,而非洲多数国家的热带果树中,芒果是最为普遍的果树之一。通过在尼日利亚东北区的包奇市从事芒果技术工作的3年中,对于芒果促花保果的措施进行了初步的探索。经过试验基本明确了在类似包奇市的生态区域内,进行环割是芒果树上的一项促花保果的关键措施,环割的时期以9月上、中旬为宜。 相似文献
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