紫花苜蓿GDP解离抑制蛋白基因cDNA的克隆、分析及烟草转化   总被引：2，自引：2，他引：0  

乌艳红  米福贵  李志明  栾守泉  陈玲玲  娜日苏  梁庆伟 《草地学报》2011,19(1):157-163

采用RT-PCR方法,克隆得到紫花苜蓿(Medicago sativa L.)GDP解离抑制蛋白基因cDNA核心区域序列,命名为MsGDI1。序列分析结果表明,该cDNA核心区域全长1575 bp,包含一个1332 bp的最大开放阅读框,编码444个氨基酸。半定量RT-PCR分析结果表明,在盐胁迫和铝胁迫条件下,MsGDI1基因的表达随着胁迫时间的增加而上升,该基因可能与紫花苜蓿抗盐耐铝机理有关。借助于新构建的该基因超表达载体,用农杆菌介导方法转化烟草(Nicotiana tabacum L.)。转基因烟草植株PCR检测的结果表明,MsGDI1基因已经成功插入到烟草基因组中,为进一步研究该基因的功能奠定了基础。  相似文献   

基于土壤剖面质地构型的土壤质量评价——以河南省封丘县为例   总被引：2，自引：0，他引：2  

李开丽  檀满枝  密术晓  陈杰 《土壤》2016,48(6):1253-1260

土壤剖面质地构型是土壤质量和土壤生产力的重要影响因子,对冲积平原地区来讲尤其重要。然而,现有的土壤质量评价研究中,很少采用该因子,且评价指标筛选大多缺乏明确的生物学意义。本研究利用河南省封丘县39个剖面数据及113个土钻样点数据,使用模糊C均值聚类方法 (FCM)结合Kriging插值方法获取的9种土壤剖面质地构型在空间上分布的单一类别隶属度图;在此基础上结合专家知识,针对平原地区旱作作物及耕地的耕性对土壤剖面质地构型的性状进行打分,以分值为参照,在Arc GIS 9.2中9个单一类别隶属度栅格文件进行栅格计算,获取了研究区土壤剖面质地构型栅格的综合分值。利用包括土壤剖面质地构型在内的多个土壤属性,结合作物产量数据,使用主成分分析(PCA)方法确定了最小数据集(MDS);应用指数和法,基于两种途径对土壤质量进行了评价,并结合测产获取的作物产量对两种评价结果进行了对比分析。结果表明:土壤质量指数与作物产量有较好的相关性,土壤质量评价结果能够较好地反映研究区的土壤质量状况。在添加剖面质地构型因子后,土壤质量评价结果有进一步的改善,可见在黄河泛滥冲积平原地区,剖面质地构型是土壤质量评价必不可少的因子。  相似文献   

Hydroxytyrosol 4-beta-D-glucoside,an important phenolic compound in olive fruits and derived products     

Romero C  Brenes M  García P  Garrido A 《Journal of agricultural and food chemistry》2002,50(13):3835-3839

There is increasing interest in olive polyphenols because of their biological properties as well as their contribution to the color, taste, and shelf life of olive products. However, some of these compounds remain unidentified. It has been shown that hydroxytyrosol 4-beta-D-glucoside (4-beta-D-glucosyl-3-hydroxyphenylethanol) coeluted with hydroxytyrosol [(3,4-dihydroxyphenyl)ethanol] under reversed phase conditions in the phenolic chromatograms of olive pulp, vegetation water, and pomace of olive oil processing. A method to separate this compound from hydroxytyrosol by HPLC has been developed. The concentration of this glucoside increased in olive pulp with maturation and could be the main phenolic compound in mature olives. In contrast, the presence of this compound was not detected in olive oil by using HPLC-MS. The compound must be considered both in table olives and olive oil processing because of its glucose and hydroxytyrosol contribution to these products.  相似文献   

Polyphenol changes during fermentation of naturally black olives     

Romero C  Brenes M  García P  García A  Garrido A 《Journal of agricultural and food chemistry》2004,52(7):1973-1979

The individual evolution of phenolic compounds has been studied during the natural fermentation of black olives for the first time. Cyanidin 3-rutinoside and cyanidin 3-glucoside were the main anthocyanins identified in fresh olives, and they were not detected after 1 month of storage either in brine or in olive. The fruit colors were different when aerobic or anaerobic conditions were used and as a consequence of the different anthocyanin polymerizations that took place. At time zero, the polyphenols observed in the olive juice were hydroxytyrosol-4-beta-glucoside, oleuropein, hydroxytyrosol, tyrosol, salidroside, and verbascoside and, after 12 months, the main phenol was hydroxytyrosol. The polyphenol content in the oil phase of olives was also analyzed. The dialdehydic form of elenolic acid linked to hydroxytyrosol and tyrosol, oleuropein aglycon, and ligstroside aglycon were the main compounds found at the beginning of fermentation but were not detected after 3 months. In contrast, hydroxytyrosol, hydroxytyrosol acetate, tyrosol, and tyrosol acetate were the main polyphenols detected in the oil phase of the final product. The acid hydrolysis of the initial glucosides (in olive juice) and the aglycons (in oil phase) was, therefore, the main reaction that took place during fermentation.  相似文献   

侵犯商业秘密罪的认定     

米镝 《郑州牧业工程高等专科学校学报》2005,25(3):204-206

商业秘密作为一种有竞争力的无形财产,越来越受到人们的关注,1997年修订的<中华人民共和国刑法>(以下简称<刑法>)将部分侵犯商业秘密行为从民事侵权行为上升为刑事犯罪行为即是明注.  相似文献   

A multilayer cell-culture technique to improve yields of a herpesvirus of turkeys     

C Garrido 《Avian diseases》1972,16(5):1087-1093

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Seed losses at harvest and seed persistence of oilseed rape (Brassica napus) in different cultural conditions in Chinese farming systems     

Y M ZHU  Y D LI  N COLBACH  K P MA  W WEI  X C MI 《Weed Research》2012,52(4):317-326

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几种植物生长调节物质对桃花粉萌发效应的影响   总被引：1，自引：0，他引：1  

米银法  王进涛  胡海渴 《西南农业学报》2012,25(3):1023-1026

采用花粉离体液体培养法研究了6种植物生长调节物质对毛桃(Amygdalus persica Linn.var.scleropersica(Reich.)YüetLu)花粉萌发的影响。结果表明,较低浓度的GA3能促进毛桃花粉的萌发,超过一定浓度时起抑制作用,并且随浓度的增大抑制作用增强,而其最适浓度是100 mg/L;NAA对毛桃花粉萌发呈现抑制作用,随着浓度的增大,抑制作用增强;ABA对毛桃花粉萌发的抑制作用也是随着浓度的增大而增强;6-BA促进花粉萌发的浓度范围是12.5～25 mg/L,而其最适浓度为12.5 mg/L,超过25mg/L时,抑制作用逐渐增强;TRIA促进毛桃花粉萌发的最适浓度为1 mg/L,超过该浓度花粉萌发率呈下降趋势;水杨酸促进萌发浓度为0.002～0.008 mmol/L,而最适浓度为0.002 mmol/L,当浓度超过该范围时,抑制作用增强。  相似文献   

珍珠岩配比基质结合浇灌营养液对番茄穴盘幼苗生长发育和根际环境的影响     

米国全  程志芳  赵肖斌  王晋华 《河南农业科学》2012,41(12)

以番茄品种中杂9号(晚熟品种)和中杂109(早熟品种)为试材,研究了珍珠岩+蛭石和草炭+蛭石(体积比均为2∶1)2种基质及其结合浇灌营养液对番茄穴盘幼苗生长发育、根际EC值和pH值的影响。结果表明,与基质草炭+蛭石相比,珍珠岩+蛭石营养条件较差,不利于番茄穴盘幼苗的生长。而珍珠岩+蛭石+营养液(ZZS)条件下培育的中杂109番茄穴盘幼苗壮苗指数(0.044 4)、G值(0.026 6)、花蕾长度(0.94cm)、叶片叶绿素含量(1.303mg/g),分别高出草炭+蛭石+营养液(CZS)培育的穴盘幼苗33.7%、35.0%、9.3%、2.0%,根际EC值(0.45mS/cm)和pH值(7.10)亦适合番茄幼苗生长。ZZS比CZS更有利于增加中杂9号和中杂109番茄幼苗的株高和茎粗。因此,以珍珠岩+蛭石(2∶1)基质配比结合浇灌营养液更有利于培育番茄穴盘壮苗。  相似文献   

薄膜和太阳能保温技术在草莓高架栽培中的应用     

糜林  霍恒志  万春雁 《西北农林科技大学学报(自然科学版)》2012,40(4):121-126

【目的】研究不同薄膜和太阳能保温技术在草莓高架栽培中的保温效果,并与地面高垄栽培相比较,寻找最佳的保温组合,为草莓高架式栽培在我国的推广奠定基础。【方法】以优良草莓品种“红颜”为试材,以地面高垄栽培为对照(CK),对高架栽培设5个薄膜保温处理（无薄膜帘披垂(CK1),外帘黑色薄膜、无内帘(T1),外帘白色薄膜、无内帘(T2),外帘白色薄膜、内帘黑色薄膜(T3),外帘黑色薄膜、内帘白色薄膜(T4）),对其中保温效果最好的处理进行太阳能热水保温（T5）,测定不同处理不同时间点(06:00开始,每隔3 h测定1次,连续测定3 d)的基质和土壤下0.05,0.10,0.15和0.20 m的温度及各处理的室内温度（CK、CK1的温室内温度,T1～T4的帘内温度）,并测定各处理果实的单果质量、最大单果重量、单株产量、可溶性固形物含量和硬度。【结果】各薄膜保温处理在09:00-18:00的保温效果差异较小,但在保温要求较高的21:00-次日06:00,各处理的保温效果差异较大,其中T3处理不同深度的基质温度均高于或显著高于其他处理,其基质的平均温度为18.4 ℃,室内平均温度为17.4 ℃。在对温度要求较高的18:00-次日06:00,T5处理不同深度的基质温度及室内温度明显高于其他处理,二者呈一定的正相关关系。T3、T5 处理的草莓果实经济性状均显著高于CK。【结论】T3处理（外帘白色薄膜、内帘黑色薄膜）对冬季草莓高架栽培的保温效果最佳;冬季低温期,利用滴管输送太阳能热水对基质増温效果明显。采取披垂薄膜帘和输送太阳能热水这2种措施能解决冬季草莓高架栽培中的保温问题,可以确保草莓在寒冷的冬季能正常生长发育。  相似文献