Method comparison of targeted influenza A virus typing and whole-genome sequencing from respiratory specimens of companion animals     

Patrick K. Mitchell  Brittany D. Cronk  Ian E. H. Voorhees  Derek Rothenheber  Renee R. Anderson  Timothy H. Chan  Brian R. Wasik  Edward J. Dubovi  Colin R. Parrish  Laura B. Goodman 《Journal of veterinary diagnostic investigation》2021,33(2):191

Epidemics of H3N8 and H3N2 influenza A viruses (IAVs) in dogs, along with recognition of spillover infections from IAV strains typically found in humans or other animals, have emphasized the importance of efficient laboratory testing. Given the lack of active IAV surveillance or immunization requirements for dogs, cats, or horses imported into the United States, serotype prediction and whole-genome sequencing of positive specimens detected at veterinary diagnostic laboratories are also needed. The conserved sequences at the ends of the viral genome segments facilitate universal amplification of all segments of viral genomes directly from respiratory specimens. Although several methods for genomic analysis have been reported, no optimization focusing on companion animal strains has been described, to our knowledge. We compared 2 sets of published universal amplification primers using 26 IAV-positive specimens from dogs, horses, and a cat. Libraries prepared from the resulting amplicons were sequenced using Illumina chemistry, and reference-based assemblies were generated from the data produced by both methods. Although both methods produced high-quality data, coverage profiles and base calling differed between the 2 methods. The sequence data were also used to identify the subtype of the IAV strains sequenced and then compared to standard PCR assays for neuraminidase types N2 and N8.  相似文献   

Degradation of the coffee flavor compound furfuryl mercaptan in model Fenton-type reaction systems     

Blank I  Pascual EC  Devaud S  Fay LB  Stadler RH  Yeretzian C  Goodman BA 《Journal of agricultural and food chemistry》2002,50(8):2356-2364

The stability of the coffee flavor compound furfuryl mercaptan has been investigated in aqueous solutions under Fenton-type reaction conditions. The impact of hydrogen peroxide, iron, ascorbic acid, and ethylenediaminetetraacetic acid was studied in various combinations of reagents and temperature. Furfuryl mercaptan reacts readily under Fenton-type reaction conditions, leading to up to 90% degradation within 1 h at 37 degrees C. The losses were lower when one or more of the reagents was omitted or the temperature decreased to 22 degrees C. Volatile reaction products identified were mainly dimers of furfuryl mercaptan, difurfuryl disulfide being the major compound. In addition, a large number of nonvolatile compounds was observed with molecular masses in the range of 92-510 Da. The formation of hydroxyl and carbon-centered radicals was indicated by electron paramagnetic resonance spectra using alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone or 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide as spin traps. Whereas *OH was generated by Fenton-type reactions, the C-centered radical is probably a secondary product of the reaction of *OH with various organic molecules, the reaction with furfuryl mercaptan appearing to be the most important. No evidence for S-centered radicals was seen in the spin-trapping experiments, but a sulfur-containing radical was detected when measurements were made at 77 K in the absence of spin traps.  相似文献   

Identification of resistance to the Ga1-m gametophyte factor in maize     

Zachary G. Jones  Major M. Goodman  Matthew D. Krakowsky 《Euphytica》2015,206(3):785-791

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Oral biology and conditions of amphibians.     

Gidona Goodman 《Veterinary Clinics of North America: Exotic Animal Practice》2003,6(3):467-75, v

This article reviews the anatomy of the skull and oral cavity in the three amphibian orders. Feeding behavior and feeding physiology are also described. A review is given of reported oral pathology. The pathology is divided into infectious causes (bacterial and parasitic) and noninfectious causes (trauma, neoplasia, and nutritional).  相似文献   

Last occurrence and survival during winter of the arbovirus vector Culicoides brevitarsis at the southern limits of its distribution     

AL BISHOP  IM BARCHIA  AM HARRIS 《Australian veterinary journal》1995,72(2):53-55

SUMMARY: Linear regression analysis was used to describe the decline in numbers of Culicoides brevitarsis Kieffer into winter with monthly maximum, average and minimum temperatures at the southern limits to its distribution in New South Wales. From this, low temperature thresholds were derived when C brevitarsis would be absent from the field. The low minimum threshold ± 2 SE (95% Confidence Interval) of 8.1 ± 0.3°C was used with historical temperature data to estimate the last month that the species should occur (March to June) and the mean number of months (2 to 6.5) below the threshold at 17 selected sites. Probability for survival during winter at these sites was estimated from years when the number of consecutive months below the threshold was ≤ 2 months. This varied from zero to 51% depending on the location of the site. Last occurrence was 0.7 months later on average and absolute probabilities for survival ranged from zero to 100% when the temperatures were increased by an arbitrary 2°C.  相似文献   

Reasons for liking and choosing a cat as a pet     

AL PODBERSCEK  JK BLACKSHAW 《Australian veterinary journal》1988,65(10):332-333

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Efficacy of erythromycin compared with penicillin/streptomycin for the treatment of virulent footrot in sheep   总被引：2，自引：0，他引：2  

JK WEBB WARE  CJ SCRIVENER  AL VIZARD 《Australian veterinary journal》1994,71(3):88-89

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Gynaecomastia and galactorrhea in a goat buck     

RAM AL JASSIM  WA KHAMAS 《Australian veterinary journal》1997,75(9):669-670

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Collection of endometrial cells in the mare     

M. BOURKE  J N. MILLS  AL BARNES 《Australian veterinary journal》1997,75(10):755-758

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Electrophoretic and serological comparisons of pathogenesis-related (b) proteins from different plant species     

L. C. van Loon  S. Gianinazzi  R. F. White  Y. Abu-Jawdah  P. Ahl  J. F. Antoniw  T. Boller  A. Camacho-Henriquez  V. Conejero  J. C. Coussirat  R. N. Goodman  E. Maiss  P. Redolfi  T. M. A. Wilson  Y. A. M. Gerritsen  H. J. Van Telgen  E. J. Van Der Zaal 《European journal of plant pathology / European Foundation for Plant Pathology》1983,89(6):293-303

Preparations of pathogenesis-related (b) proteins (PRs) from differentNicotiana species, tomato,Gynura aurantiaca, bean, and cowpea were compared to each other and to bean chitinase and a constitutive apple agglutinin by electrophoresis in polyacrylamide gels both in the absence and in the presence of SDS, and by serological double diffusion analysis using antisera against tobacco PRs and bean chitinase. PRs from different plant genera displayed a similar but not identical range of relative mobilities in both native and SDS gels, whereas bean chitinase and apple agglutinin were clearly different. None of the antisera reacted with any of the PR preparations from plant genera other than the one from which the antigen(s) had been derived. Whilst PRs within the genusNicotiana are serologically related and can be identical, PRs from different plant genera seem to be sufficiently different to be considered as genus-specific.  相似文献