Effect of Complex Vertebral Malformation on Luteal Function in Holstein Cows During Oestrous Cycle and Early Pregnancy     

ME Ghanem  T Suzuki  A Kasuga  M Nishibori 《Reproduction in domestic animals》2010,45(4):729-733

The reason why cows carrying the mutation of complex vertebral malformation (CVM) show poor reproductive capability although they carry only one mutant allele is still not fully understood. Monitoring the progesterone profiles during oestrous cycle and early pregnancy in carrier cows might help explain their lowered reproductive capability. Progesterone concentration was measured in 19 CVM carrier cows and 21 control cows during oestrous cycle and early pregnancy. Milk samples were collected from all cows starting on the day of artificial insemination until day 45 post‐AI. Progesterone was measured in skim milk using enzyme‐linked immunosorbent assay (ELISA). Progesterone concentration was significantly reduced on day 7 (p < 0.05) and day 9 (p < 0.01) post‐insemination in conceived CVM carrier cows when compared with that in control conceived cows. The mean progesterone concentration during early pregnancy was significantly lower (p < 0.05) in conceived cows with CVM than that of control cows in the same period. However, the mean progesterone concentration did not differ significantly (p = 0.072) in CVM cows that showed fertilization failure or embryonic death than that of control cows. Additionally, of 13 conceived control cows, eight cows (61.5%) showed normal luteal function. In contrast, of nine conceived CVM cows, only four cows (44.4%) showed normal luteal function. The conception rate was 47.4% in CVM carrier cows and 61.9% in control cows, but this difference did not reach significance. In conclusion, progesterone concentration might be lowered during early pregnancy in conceived CVM cows compared with that in control cows.  相似文献   

青花菜不同器官中4-甲基亚磺酰丁基硫苷及萝卜硫素含量分析   总被引：2，自引：0，他引：2  

姚雪琴  谢祝捷  李光庆  邱海荣  李媛 《中国农业科学》2011,44(4):851-858

 【目的】青花菜的抗癌功能主要与4-甲基亚磺酰丁基硫苷的降解产物萝卜硫素有关,通过测定不同青花菜材料花球、茎和叶片中两者的含量并分析两者含量间的相关性,为青花菜高营养品质育种提供理论依据。【方法】采用反相高效液相色谱法,对24个不同青花菜材料花球、茎和叶片中4-甲基亚磺酰丁基硫苷含量,及其中的10份材料花球、茎和叶片中萝卜硫素含量进行测定和分析。【结果】青花菜不同基因型间以及不同器官间4-甲基亚磺酰丁基硫苷和萝卜硫素含量差异均极显著,4-甲基亚磺酰丁基硫苷和萝卜硫素含量均为花球含量最高,其次为茎,叶片含量最低;不同材料花球中4-甲基亚磺酰丁基硫苷平均含量分别为茎和叶片的4.4和13.97倍,萝卜硫素平均含量分别为茎和叶片的4和8.7倍,而有的基因型材料茎和叶片中4-甲基亚磺酰丁基硫苷的含量较高,约为花球中的50%;参试的10个不同基因型材料的花球、茎和叶片中的萝卜硫素含量与其前体4-甲基亚磺酰丁基硫苷含量间相关系数分别为0.9886**,0.9994**,0.9935**。【结论】不同青花菜材料间不同器官中4-甲基亚磺酰丁基硫苷含量存在显著差异;在青花菜萝卜硫素开发应用中,可选择其前体4-甲基亚磺酰丁基硫苷作为筛选指标,并可筛选茎和叶中4-甲基亚磺酰丁基硫苷含量较高的材料作为提取萝卜硫素的原料。  相似文献   

The Role of the Antebrachiocarpal Ligaments in the Prevention of Hyperextension of the Antebrachiocarpal Joint     

Joshua Milgram BVSc  Diplomate ECVS  Tomer Milshtein DVM  Yaron Meiner BSc ME 《Veterinary surgery : VS》2012,41(2):191-199

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早熟禾草坪中牛筋草的化学防除   总被引：1，自引：0，他引：1  

武菊英  王庆海 《草原与草坪》2004,(3):36-38

盆栽条件下研究了牛筋草(Eleusineindica)在早熟禾(Poapratensis)草坪不同生育期的防治效果及对早熟禾生长的影响。结果表明早熟禾出苗前,牛筋草在2～3叶期喷施草甘膦450mL/hm2,对牛筋草的防效达98%,该剂量对早熟禾的出苗和生长无不良影响。早熟禾4叶期以上,牛筋草3叶期时,使用坪绿6号600mL/hm2可有效防除牛筋草,防效达90.5%,但该剂量下对早熟禾生长有抑制作用。添加助剂SDP,OP 10和PPJ 15可显著提高坪绿6号对牛筋草的防除效果,对坪绿6号低剂量(150L/hm2)的提高幅度,高于对高剂量的(300和600mL/hm2)。已经成坪的早熟禾中,在牛筋草出苗前,喷施坪绿5号3000mL/hm2,在30d内对牛筋草的防效达87%～92%,对早熟禾生长无不良影响。  相似文献   

Endocrinological studies on parabiotic rats with TURISYNCHRON (Metallibur) and TURISYNCHRON-Z. 1. Findings in intact parabionts     

M Oettel  W St?lzner  B Zieger  M Grass  K H Chemnitius 《Archiv fuer experimentelle veterinaermedizin》1972,26(6):939-947

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Pharmacologic-endocrinological findings in animal experiments with TURISYNCHRON and SUISYNCHRON. 1. Effect on gonadotropic function of the rat hypophysis     

W St?lzner  M Grass  K H Chemnitius 《Archiv fuer experimentelle veterinaermedizin》1974,28(5):641-649

The antigonadotrophic activity of the metallibure preparation TURISYNCHRON and its zinc complex SUISYNCHRON was investigated in rats by measuring inhibition of testicular growth, inhibition of ovulation and effect on the oestrous cycle. The zinc complex was less active in the ovulation inhibition test, in contrast to the testicular inhibition test, and this was attributed to its peculiar mode of absorption.  相似文献   

BSAVA Manual of Small Animal Ophthalmology, 2nd edn.     

ME Bernays 《Australian veterinary journal》2004,82(7):416-416

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Role of Cumulus Cells on In Vitro Maturation of Canine Oocytes     

T Otoi  T Shin  DC Kraemer  ME Westhusin 《Reproduction in domestic animals》2007,42(2):184-189

The objectives of the present study were to investigate the relationship between the morphological status of cumulus cells surrounding canine oocytes after maturation culture and the meiotic stage of the oocytes. In addition, the effect of the removal of cumulus cells from canine cumulus-oocyte complexes (COCs) during maturation culture on their meiotic competence was examined. Canine COCs were collected from bitches at the anoestrous and dioestrous stages and only COCs with >110 microm in vitelline diameter were cultured in medium 199 with 10% canine serum for 72 h. In the first experiment, the relation between the morphological status of cumulus cells surrounding oocytes cultured for 72 h and their meiotic stages was examined. At the end of maturation culture, the proportions of intact, partially nude and completely nude oocytes were 65.2%, 22.9% and 11.9%, respectively. The proportion of maturation to metaphase II of completely nude oocytes was highest among the oocytes with different morphological status of cumulus cells. In the second experiment, the cumulus cells were partially or completely removed from COCs at 48 h after the start of maturation culture and the oocytes were cultured for a further 24 h. The proportion of oocytes reaching metaphase II in the completely denuded oocytes was significantly higher than that in the control oocytes without the removal treatment of cumulus cells. The results indicate that morphological status of cumulus cells surrounding oocytes may be related to the nuclear maturation of canine oocytes, and the removal of cumulus cells from COCs during maturation culture can promote the completion of oocyte meiotic maturation.  相似文献   

Methyl‐β‐Cyclodextrin Improves Sperm Capacitation Status Assessed by Flow Cytometry Analysis and Zona Pellucida‐Binding Ability of Frozen/Thawed Bovine Spermatozoa          下载免费PDF全文

L Águila  ME Arias  T Vargas  F Zambrano  R Felmer 《Reproduction in domestic animals》2015,50(6):931-938

Mammalian sperm undergo a series of biochemical transformations in the female reproductive tract that are collectively known as capacitation. Cyclodextrins added to the sperm culture medium have been described to induce in vitro sperm capacitation, enabling its use in protein‐free media. However, the additive capacitating effect of methyl‐β‐cyclodextrin (MβCD) in the medium containing bovine serum albumin (BSA) is unknown in the bovine species. In this study, we evaluated the effects of incubating frozen–thawed bovine spermatozoa in a BSA‐containing medium supplemented with MβCD on different sperm quality and functional parameters. Sperm viability decreased with the addition of MβCD in a dose‐dependent manner (p < 0.05), and DNA damage could be observed but only with the highest concentration of MβCD. However, pre‐incubation of spermatozoa in MβCD‐supplemented medium improved the capacitation status as assessed by the increase in plasma membrane fluidity, intracellular calcium concentration, induced acrosome reactivity and zona pellucida (ZP)‐binding ability (p < 0.05). Thus, we conclude that MβCD supplementation is able to enhance the capacitation status of frozen–thawed bovine spermatozoa cultured in capacitation medium containing BSA and could result in a valid strategy for its application on artificial reproductive technologies such as in vitro fertilization or intracytoplasmic sperm injection.  相似文献   

Development of a technique for standing hand‐assisted laparoscopic splenectomy in five horses          下载免费PDF全文

LA Gracia‐Calvo  M Martín‐Cuervo  J Jiménez  V Vieítez  D Argüelles  ME Durán  J Ezquerra 《Australian veterinary journal》2015,93(6):183-188

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