Gene and protein expression and cellular localisation of cytochrome P450 enzymes of the 1A, 2A, 2C, 2D and 2E subfamilies in equine intestine and liver     

Eva Tydén  Hans Tj?lve  Pia Larsson 《Acta veterinaria Scandinavica》2014,56(1)

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Effects of Reduced Fishing for Feeding Salmon (Salmo salar L.) in the Baltic on Home Water Fisheries according to Simulations with the Carlin-Larsson Population Model     

P.-O. Larsson 《Aquaculture Research》1984,15(3):97-105

The Carlin-Larsson population model of Baltic salmon was used to simulate reduced effort in an offshore fishery for feeding salmon and its effect on home water fisheries. Some simplifying assumptions had to be made; no changes in seasonal mean weight or exploitation rate in the home water fisheries were assumed. With a simulated termination of the offshore fishery, seasonal mean weights from the time before the excessive offshore fishery started were also applied to give a maximum effect on the catches. The total non-catch fishing mortality in the offshore fishery was estimated at 5% per season (total M = 10% per season). A total ban on the offshore fishery would mean a sixfold increase in catch-value for home water fisheries and a total value twice that in the present situation with the present seasonal mean weights. With the higher mean weights the simulated catch would be 87% and its value 182% higher than at present.  相似文献   

Accuracy and precision when sampling from fish troughs     

P. Seeger  U. Thorsson  B. Larsson  K. Rnningen 《Aquaculture (Amsterdam, Netherlands)》1977,10(4):365-371

Salmon have been sampled repeatedly from seven troughs in order to evaluate the accuracy and precision of three different sampling methods. The average length of 2-summer-old salmon was determined to the nearest half centimetre after using a large scoop net which caught somewhat more than 200 fish per sweep. To avoid bias we had to use weighted means, with the weightings proportional to the square roots of the lengths. For 1-summer-old salmon we could use a smaller scoop net and unweighted means.  相似文献   

Spawning induces a shift in energy metabolism from glucose to lipid in rainbow trout white muscle   总被引：1，自引：0，他引：1  

A. Kiessling  L. Larsson  K. -H. Kiessling  P. B. Lutes  T. Storebakken  S. S. S. Hung 《Fish physiology and biochemistry》1995,14(6):439-448

Enzymatic changes that occur in the white somatic muscle of rainbow trout (Oncorhynchus mykiss) in response to spawning were investigated, and the evenness of their distribution across the ventral-dorsal plane of this muscle was assessed. Four enzymes that are involved in energy metabolism were measured (phosphofructokinase: glycolytic capacity, 3-hydroxyacyl-CoA dehydrogenase: -oxidation, citrate synthase: citric acid cycle, cytochrome oxidase: oxidative capacity). The enzyme activities were followed in different parts of the white muscle of non-spawning female rainbow trout from May, four months after their first spawning, until December, at second spawning. Samples were taken from white epaxial muscle along the lateral line, on the dorsum, and in between. Samples were also taken from red muscle of non-spawning fish. The isoforms of myosin heavy chains (MyHC) were electrophoretically identified on 6% SDS-PAGE gel to study possible changes in contractile properties of the muscle.Transformation from the non-spawning to spawning phase was associated with dramatic changes in the activity of the enzymes studied in white muscle: glycolytic capacity decreased to less than half, whereas oxidative metabolism increased about two- to four-fold in all areas. Significant quantitative differences in enzyme activities were found between the three epaxial muscle areas: in the non-spawning fish lateral line samples differed from those taken in the other two areas, whereas in spawning fish the dorsal sample difered from the other two. No difference in the expression of MyHC-isoforms was found between spawning and non-spawning fish. Co-expression of both slow and fast isoforms was found in single fibres isolated from red muscle.The results show that the energy metabolism in white muscle of domestic rainbow trout is altered during spawning; i.e., the metabolism becomes increasingly aerobic, with an increased capacity for fatty acid utilization, concomitant with phenotypic changes associated with sexual maturation. These changes are especially pronounced in ventral, superficially located fibres.  相似文献   

Cetirizine in horses: pharmacokinetics and effect of ivermectin pretreatment     

Olsén L  Ingvast-Larsson C  Bondesson U  Broström H  Tjälve H  Larsson P 《Journal of veterinary pharmacology and therapeutics》2007,30(3):194-200

The pharmacokinetics of the histamine H(1)-antagonist cetirizine and the effects of pretreatment with the antiparasitic macrocyclic lactone ivermectin on the pharmacokinetics of cetirizine were studied in horses. After oral administration of cetirizine at 0.2 mg/kg bw, the mean terminal half-life was 3.4 h (range 2.9-3.7 h) and the maximal plasma concentration 132 ng/mL (101-196 ng/mL). The time to reach maximal plasma concentration was 0.7 h (0.5-0.8 h). Ivermectin (0.2 mg/kg bw) given orally 1.5 h before cetirizine did not affect its pharmacokinetics. However, ivermectin pretreatment 12 h before cetirizine increased the area under the plasma concentration-time curve by 60%. The maximal plasma concentration, terminal half-life and mean residence time also increased significantly following the 12 h pretreatment. Ivermectin is an inhibitor of P-glycoprotein, which is a major drug efflux transporter in cellular membranes at various sites. The elevated plasma levels of cetirizine following the pretreatment with ivermectin may mainly be due to decreased renal secretion, related to inhibition of the P-glycoprotein in the proximal tubular cells of the kidney. The pharmacokinetic properties of cetirizine have characteristics which are suitable for an antihistamine, and this substance may be a useful drug in horses.  相似文献   

Gas-liquid chromatographic determination of benzoic acid and sorbic acid in foods: NMKL collaborative study     

B K Larsson 《Journal of the Association of Official Analytical Chemists》1983,66(3):775-780

A gas-liquid chromatographic method for the simultaneous determination of benzoic acid and sorbic acid in foods was collaboratively studied by 8 laboratories. Benzoic and sorbic acids are isolated from food by successive extractions with ether, sodium hydroxide, and methylene chloride, converted to trimethylsilyl (TMS) esters, and determined by gas-liquid chromatography. Phenylacetic acid and caproic acid are used as internal standards for benzoic acid and sorbic acid, respectively. Seven samples were collaboratively studied: almond paste, fish homogenate, and apple juice with benzoic and sorbic acid levels from 0.04 to 2 g/kg. Average recoveries (%) for benzoic and sorbic acids were as follows: almond paste, 99.6 and 101.2; fish homogenate, 99.2 and 97.4; and apple juice 98.2 and 106.6. The reproducibility coefficients of variation (%) for benzoic and sorbic acids at 0.5-2 g/kg levels were 3.5-6.1 and 5.2-9.0; and at the 0.04 g/kg level, 14.7 and 23.3, respectively. The method has been adopted official first action at 0.5-2 g/kg levels.  相似文献   

Angiogenic properties of Kaposi's sarcoma-derived cells after long-term culture in vitro   总被引：31，自引：0，他引：31  

S Z Salahuddin  S Nakamura  P Biberfeld  M H Kaplan  P D Markham  L Larsson  R C Gallo 《Science (New York, N.Y.)》1988,242(4877):430-433

Cells derived from lung biopsies and pleural effusions from AIDS patients with Kaposi's sarcoma (KS) of the lungs were established in long-term culture with the aid of conditioned medium from HTLV-II-transformed T cells (HTLV-II CM). These AIDS-KS cells were similar to the so-called spindle cells in KS lesions and had some of their features. They produced factors that supported their own growth (autocrine) and the growth of other cells (paracrine), including umbilical vein endothelium and fibroblasts. That the AIDS-KS cells also expressed potent angiogenic activity was demonstrated by the chorioallantoic membrane assay and by subcutaneous inoculation of AIDS-KS cells into nude mice, which resulted in the development of angiogenic lesions composed of mouse cells and showing histological features similar to those of human KS lesions. These data suggest that AIDS-associated KS and possibly other types of KS may be initiated by signals that induce the growth of particular cells (spindle cells of lymphatic or vascular origin) and the expression of autocrine and paracrine activities.  相似文献   

Response     

Sereno PC  Wilson JA  Larsson HC  Dutheil DB  Sues HD 《Science (New York, N.Y.)》1995,267(5205):1752-1753

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Predatory Dinosaurs from the Sahara and Late Cretaceous Faunal Differentiation     

PC Sereno  DB Dutheil  M Iarochene  HCE Larsson  GH Lyon  PM Magwene  CA Sidor  DJ Varricchio  JA Wilson 《Science (New York, N.Y.)》1996,272(5264):986-991

Late Cretaceous (Cenomanian) fossils discovered in the Kem Kem region of Morocco include large predatory dinosaurs that inhabited Africa as it drifted into geographic isolation. One, represented by a skull approximately 1.6 meters in length, is an advanced allosauroid referable to the African genus Carcharodontosaurus. Another, represented by a partial skeleton with slender proportions, is a new basal coelurosaur closely resembling the Egyptian genus Bahariasaurus. Comparisons with Cretaceous theropods from other continents reveal a previously unrecognized global radiation of carcharodontosaurid predators. Substantial geographic differentiation of dinosaurian faunas in response to continental drift appears to have arisen abruptly at the beginning of the Late Cretaceous.  相似文献   

Nutrient and persistent pollutant deposition on the Bothnian Bay ice and snow fields     

Lars Rahm  Bertil Håkansson  Per Larsson  Elisabet Fogelqvist  Gudrun Bremle  Jorge Valderrama 《Water, air, and soil pollution》1995,84(1-2):187-201

A study of atmospheric deposited nutrients and some persistent pollutants has been carried out on the ice and snow fields of the subarctic Bothnian Bay, the northernmost basin of the Baltic Sea. Total amounts of 600, 500 and 400 tons NO₃-N, NH₄-N and N_org-N, respectively are deposited in the snow while the corresponding amounts for P_tot-P is 40 tons. The corresponding amount for PCB and lindane are 1.0 and 0.2 kg, respectively. The measurements were carried out on the snow-covered ice four to six weeks old. A part of the deposited snow has been incorporated -into the snow-ice and an attempt to estimate its mean thickness and its amount of nutrients has been made. The total amounts now reach 1700, 1300 and 1100 tons of NO₃-N and NH₄-N and N_org-N, respectively in the snow and ice together. The results obtained support the use of land-based stations in estimates of seasonal atmospheric nutrient deposition to the Bothnian Bay. The observed concentrations of chloroorganic compounds correspond to those land-based observations reported from the same latitude in the northern hemisphere and reported in literature.  相似文献