Research Lactobacillus rhamnosusFermented Herbal and Bacillus subtilis Complex on Immune Performance,E.coli Infection of Broiler     

XIE Quan-xi  ZHANG Jian-mei  YU Jia-min  QI Xiu-ye  XU Hui  XU Hai-yan  GU Wei 《中国畜牧兽医》2016,43(6):1523-1529

This experiment was designed to study the complex of Lactobacillus rhamnosus fermented herbal and Bacillus subtilis on White Feather broiler immunity performance and impact of Escherichia coli infection.360 one-day-old broiler chickens were randomly divided into 3 groups with 4 replicates in each group and 30 chickens per replicate.The pretrial period lasted for 7 d,and the experiment lasted for 35 d.The chickens in the group Ⅰ(positive control group) and group Ⅱ(negative control group)were all only fed a basal diet,group Ⅲ was test group,by additive 1% fermented herbal preparations,groups Ⅱ and Ⅲ were intraperitoneal injection of 1 mL E.coli at 35 d,broiler mortality,immune organ index,cecalmicroflora content,immunoglobulin levels,IL-2 and IL-6 content were tested.The results showed that injection of E.coli caused massive death of chickens,group Ⅱtook up to 75.00%,it was significantly higher than groups Ⅰ and Ⅲ (P < 0.05),the mortality in group Ⅲ was significantly lower than group Ⅱ(P < 0.05),was only 23.33%.Injection of E.coli maked spleen index and thymus index of group Ⅱincreased significantly (P < 0.05),the spleen index and thymus index of groups Ⅰ and Ⅲ were no significant difference (P > 0.05).E.coli counts was significantly decreased after injectionin group Ⅲ (P < 0.05),but the number of intestinal Lactobacilli of group Ⅲ was significantly increased (P < 0.05),and inhibited the propagation of E.coli,the counts of E.coli in groups Ⅰ and Ⅲ were no significant difference (P > 0.05).At 42 d,the sIgA of the intestinal fluid in group Ⅲ were higher than that of groups Ⅰ and Ⅱ with 11.99% and 36.56%,respectively(P < 0.05).The serum IgG concentrations of group Ⅲ was higher than that of groupsⅠand Ⅱwith 14.68% and 28.15%,respectively(P < 0.05).At 42 d,the IL-2 content of group Ⅱ was the lowest,it was significantly lower than group Ⅲ(P < 0.05),the IL-6 of group Ⅲ was significantly lower than group Ⅱ(P < 0.05).  相似文献   

东北地区奶牛乳房炎表皮葡萄球菌和腐生葡萄球菌的分子流行病学及耐药性研究     

董志民  张万江  刘琪  卢中冶  王秋东  华欣  韩吉雨  宋丽华  刘思国 《中国预防兽医学报》2016,(4):307-311

为调查表皮葡萄球菌和腐生葡萄球菌在东北地区的流行病学情况和耐药性,本研究对来自东北地区3个大型奶牛场采集的330份奶样进行葡萄球菌的分离、鉴定及其耐药表型的检测,并采用脉冲场凝胶电泳(PFGE)进行分离株的亲缘性分析,对表皮葡萄球菌进行多位点序列分型(MLST),同时应用PCR扩增分离株中携带的相关耐药基因。研究结果表明,在330份奶样中共分离到表皮葡萄球菌32株(9.7%),腐生葡萄球菌34株(10.3%);PFGE分析共获得9种不同谱型的表皮葡萄球菌和11种不同谱型的腐生葡萄球菌。药敏试验结果显示,两种菌对青霉素(70%)、苯唑西林(60%)和林克霉素(55%)的耐药率较高,主要耐药基因为lnu(B)(40%)、erm(B)(30%)和mec A(25%)。本研究结果揭示了东北地区奶牛乳房炎病原菌表皮葡萄球菌和腐生葡萄球菌的耐药谱和流行情况,为临床合理用药及奶牛乳房炎的防控提供了实验依据。  相似文献   

苹果树种植对黄土旱塬土壤蒸发的影响     

华溢  陆彦玮  李敏  韦昊延  王建龙  庞薛清秋  司炳成 《干旱地区农业研究》2023,(5):51-58

以陕西长武旱塬为例,分别对研究区农地和5个不同林龄(9、12、16、19 a和23 a)苹果园的土壤剖面氢氧稳定同位素进行测定,利用Craig-Gordon模型定量估算其土壤平均蒸发量,并基于“空间换时间”方法分析果园种植及生长对土壤蒸发的影响。结果表明：农地及9、12、16、19、23 a林龄苹果园的土壤蒸发量均随苹果树的种植及生长呈现先减少再增大的趋势,年均蒸发量分别为129、104、89、119、128、136 mm;苹果园的土壤蒸发量变化与叶面积指数呈显著负相关(R=-0.713);苹果园种植的前中期(9～12 a)土壤蒸发量随叶面积指数增加逐渐减小,而在中后期(12～23 a)深层土壤水被大量消耗造成的干旱胁迫使得果树叶面积减少,从而导致林下土壤蒸发量又逐渐增大。  相似文献   

Using genetic tools to inform conservation of fragmented populations of Asian elephants (Elephas maximus) across their range in China     

Ying CHEN  Yakuan SUN  Mei HUA  Kun SHI  David DUDGEON 《Integrative zoology》2023,18(3):453-468

A herd of 15 Chinese elephants attracted international attention during their 2021 northward trek, motivating the government to propose establishment of an Asian elephant national park. However, planning is hampered by a lack of genetic information on the remaining populations in China. We collected DNA from 497 dung samples from all 5 populations encompassing the entire range of elephants in China and used mitochondrial and microsatellite markers to investigate their genetic and demographic structure. We identified 237 unique genotypes (153 females, 84 males), representing 81% of the known population. However, the effective population size was small (28, range 25–32). Historic demographic contraction appeared to account for low haplotype diversity (H_d = 0.235), but moderate nucleotide and nuclear diversity (π = 0.6%, H_e = 0.55) was attributable to post-bottleneck recovery involving recent population expansion plus historical gene exchange with elephants in Myanmar, Lao PDR, and Vietnam. The 5 populations fell into 3 clusters, with Nangunhe elephants differing consistently from the other 4 populations (F_ST = 0.23); elephants from Mengyang, Simao, and Jiangcheng belonged to a single population (henceforth, MSJ), and differed from the Shangyong population (F_ST = 0.11). Interpopulation genetic variation reflected isolation by distance and female-biased dispersal. Chinese elephants should be managed as 2 distinct units: Nangunhe and another combining Shangyong and MSJ; their long-term viability will require restoring gene flow between Shangyong and MSJ, and between elephants in China and neighboring countries. Our results have the potential to inform conservation planning for an iconic megafaunal species.  相似文献   

Analysis of Social Appraisal Index System in Public Project     

HUA Yong-jun  CHEN Xun  ZHANG Jian 《保鲜与加工》2005,(7):145-147

The social evaluation theories of public project still have not been standardization today, and have effects on the process of the construction to city. One of the main reasons lays in that the index system, about the social evaluation theories of public project, connects with many fields, has many non-economic index and difficult in measuring the every index. This article is to mark off the main factors involved in social evaluation effectually, to set up index system of social evaluation practically, and to settle firm ground for methodology and theory of public project.  相似文献   

播种检测技术的研究进展与趋势     

郑一平  花有清 《农机化研究》2005,(1):31-33

播种作业过程中,一旦出现播种异常现象应能及时报警,并检测出故障的性质和位置,以便机手排除故障,提高播种机的播种质量。要实现这一目的,就必须对播种性能施全程监控。为此,介绍了播种检测技术发展概况,指出了提高精密播种机田间播种质量的关键问题,分析了播种检测技术的发展趋势。  相似文献   

农业"三新技术"农机化配套项目的应用     

梁中华  华桥申  徐胜利  王吉善  俞党 《中国农机化》2006,(2):38-39

为进一步提高粮食生产科技含量,稳定粮食生产能力,近几年来浙江省确定在全省667khm2商品粮基地中实施以农业新品种、新技术和新农机为主要内容的“三新技术”配套项目。上虞市于2002年度获得了该项目的建设计划,并按照省定要求,联系本地实际,及时制定了项目实施方案,落实地方配套资金,签订项目承包合同,加强项目的管理,确保了项目的建设质量,产生了良好的社会和经济效益。该项目的实施既促进了农业龙头企业的生产发展,又起到了较好的示范和带动作用,也为提高上虞市部分农产品的经济效益提供了较好的条件,有着广阔的市场发展前景。  相似文献   

不同播期夏播小豆产量性能动态指标与光温水效应     

王乐政  华方静  曹鹏鹏  高凤菊  夏文荣 《草业学报》2021,30(1):116-129

为探讨光、温、水等气象因子对夏播小豆产量的影响,达到合理利用气象资源、进一步挖掘小豆增产潜力的目的,以中晚熟小豆品种花小豆(V1)和中早熟品系德红5261(V2)为材料,设T1(6月17日)、T2(6月22日)、T3(6月27日)、T4(7月2日)和T5(7月7日)5个播期,形成不同的光、温、水生长环境,对夏播小豆产量...  相似文献   

用意杨压缩木替代传统木梳材料的研究     

吴琼  华毓坤 《林产工业》2006,33(3):27-29

我国木梳素以小叶黄杨、楠木、枣木、梨木等树种为制梳材料。由于人口膨胀,木梳需求量巨增,制梳原材料渐已匮乏,绵延1 500多年的传统木梳手工艺文化正面临消亡的威胁。笔者选择速生树种——意杨为制梳材料,按木梳实用功能和产业经济的实际需求,对意杨压缩木进行一般物理特性试验,对其木梳制品进行实用和成本分析,最终确定50％左右的意杨压缩木完全能替代木梳传统原材料。  相似文献   

HPLC-PDA法测定兽用中药液体制剂中非法添加的四种解热镇痛类药物     

徐汀  张亦菲  黄家莺  张浩然  田恺  华贤辉  曹莹 《中国兽药杂志》2021,55(3):30-37

实验建立了兽用中药液体制剂中非法添加对乙酰氨基酚、安乃近、氨基比林、安替比林四种解热镇痛类药物的高效液相色谱-二极管阵列(HPLC-PDA)检测方法.方法采用CAPCELL PAK MGⅡS5 C18(4.6 mm×150 mm,5μm)色谱柱,柱温30℃,流动相为磷酸盐缓冲液和乙腈(84:16),流速1.0 mL/m...  相似文献