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81.
82.
The most significant focal points of the embryo transfer technology are as follows: the selection of donors, the response of the selected donor to the superovulation protocol and the obtained number of the transferable embryos. For this purpose, it is suggested that donor selection can be done by anti‐Müllerian hormone (AMH) levels, and embryo production is evaluated. AMH is secreted by the granulosa cells of primordial, pre‐antral and antral follicles below 4 mm in the ovary, independent of FSH. Therefore, the aim of this study was to investigate the relationship between serum AMH levels and the number of corpus luteum (CL), total embryos and transferable embryos that were shaped after a uniform superovulation protocol. For this reason, 48 Simmental cows, which were located at General Directory of Agricultural Enterprises (region, province, etc. instead of the general directorate), were used as donors for the embryo transfer. Blood samples were taken at random, regardless of the stage of animal's sexual cycle. AMH levels were measured by enzyme‐linked fluorescent assay (ELFA) method of the miniVIDAS® (bioMérieux SA) using AMH Bovine Test Kit. According to the statistical analyses of the obtained data, AMH levels were positively correlated with CL and total embryos (p < .05). No significant correlations between AMH and transferable embryos were approved (p > .05). It was also determined that each 200 pg/ml increase in serum AMH level resulted in one increase in CL number. Overall, considering the positive correlation between AMH level and the obtained number of CL and total embryos after a superovulation treatment, it was concluded that measuring blood AMH level prior to any further costly implementation may be an effective method in donor selection.  相似文献   
83.
Goniothalamusin and two mixtures, acetogenins-A and acetogenins-B, isolated from a petroleum ether extract of the stem bark of Miliusa velutina exhibited significant antibacterial and cytotoxic activities.  相似文献   
84.
Chowdhury R  Hasan CM  Rashid MA 《Fitoterapia》2003,74(1-2):155-158
The extracts of two Bangladeshi medicinal plants, Toona ciliata (stem bark) and Amoora rohituka (stem bark), along with siderin, a major coumarin from T. ciliata, exhibited significant in vitro antibacterial activity. The extracts also demonstrated mild antifungal effect.  相似文献   
85.
Animal bites are a major public health problem. The aim of this study was to conduct a survey of animal bites in Bursa, Turkey. Patients who sought medical care for animal bites over an 18-month period were included in our study (n = 1,715). Data were obtained from the registry of the Bursa State Hospital; 71.84% of the patients were men and 28.16% were women. Bites were most frequent among patients who were >18 years of age (comprising 60.83% of all bites). The anatomical site of the bite was recorded in all 1,715 cases, and 1,778 bite locations on the body were reported. The upper extremities were the most frequent sites of animal bites (53.83%), followed by the lower extremities (38.03%). Bites were most frequently inflicted by dogs (68.04%), followed by cats (28.45%), mice (2.56%), and other animals (hamsters, donkeys, horses, foxes, and rabbits together comprised 0.95% of the bites). As a result, bite incidents were mostly associated with males aged >18 years and occurred in the upper extremities. This study showed that dog bites constitute a major animal bite problem in Bursa, Turkey.  相似文献   
86.
Fish Physiology and Biochemistry - Recombinant expression system was established for rainbow trout myoglobin (Mb) considering its unique primary structure of having one unusual deletion and two...  相似文献   
87.
Objective To develop a simple and effective surgical technique for third‐compartment cannulation in alpacas. Design Prospective study using six adult male alpacas. Methods General anaesthesia was induced and a polyurethane gastrostomy tube was surgically implanted into the distal portion of the third compartment. Results Three of the alpacas retained their cannulas for a 100‐day period; however, three cannulas were dislodged during the study. Two of the three dislodged cannulas were replaced during a second surgical procedure. Cannulas were well tolerated by the alpacas and all animals remained clinically healthy during the study period. Third compartment contents did not leak from the cannulation site. The tubes were manually removed following the completion of the study and the small defect in the body wall quickly healed over in all animals. Conclusion Surgical placement of polyurethane tubes designed for percutaneous endoscopic gastrostomy is a useful method of cannulating the third compartment in camelids. This technique can be used for experimental studies and possibly could be used for nutritional support and fluid therapy in sick camelids that might need long‐term care.  相似文献   
88.
Exogenous estrogens used for therapeutic purposes or endogenous estrogen sources such as functional Sertoli cell or ovarian granulosa cell tumors may cause bone marrow toxicity in dogs. The condition is characterized by hematologic abnormalities including thrombocytopenia, anemia, and leukocytosis or leukopenia. Despite intensive therapy with blood or platelet-rich transfusions, broad-spectrum antibiotics, steroids, and bone marrow stimulants, prognosis is unfavorable. Due to the the risk of stimulating the development of uterine diseases and the potential for inducing aplastic anemia, estrogen use in dogs is best avoided where possible. This paper describes the causes of estrogen-induced myelotoxicity, the clinical presentation of the patients, the diagnosis, and the treatment options in the dog.  相似文献   
89.
The gamma spectrometric analysis of soil and essential foodstuffs, e.g., wheat, millet, potato, lentils and cauliflower, which form the main component of the daily diet of the local public, was carried out using high purity germanium (HpGe) detector coupled with a computer based high-resolution multi-channel analyzer. The activity concentration in soil samples for 226Ra, 232Th and 40K ranged from 30.0 Bq kg?1 to 81.2 Bq kg?1, 31.4 Bq kg?1 to 78.25 Bq kg?1 and 308.8 Bq kg?1 to 2177.6 Bq kg?1, with mean values of 56.2, 58.5 and 851.9 Bq kg?1, respectively. The average activity measured for 226Ra, 232Th and 40K in soil samples was found higher than the world average. The major radionuclide found in the food items studied was 40K, while 226Ra, 232Th and 137Cs were detected in very nominal amounts. The results clearly indicate that these radionuclides have no health hazard to human beings, as they are well below the annual limit of intake (ALI) for these radionuclides. The transfer factors of these radionuclides from soil to food were also studied. The mean transfer factors of 40K, 226Ra, 232Th and 137Cs from soil to food were estimated to be about 0.17, 0.07, 0.16 and 0.23, respectively. An artificial radionuclide, 137Cs, was also present in detectable amount in all samples. The internal and external hazard indices were measured and had mean values of 0.70 and 0.55, respectively. Absorbed dose rates and effective dose have been determined in the present study. Concentration of trace metals, such as Cr, Pb, Ni and Zn, was also determined in the soil samples. The concentrations of radionuclides and trace metals found in these samples during the present study were nominal and do not pose any potential health hazard to the general public.  相似文献   
90.
Basidiospores are implicated in the distribution and genetic diversity of Ganoderma boninense, cause of basal stem rot (BSR) and upper stem rot (USR) of oil palm (Elaeis guineensis). Measurement of aerial basidiospores within plantations in Sumatra showed continuous and high production over 24 h (range c. 2–11 000 spores m?3) with maximum release during early evening. Basidiospores applied to cut surfaces of fronds, peduncles and stems germinated in situ. Equivalent, extensive wounds are created during plantation harvesting and management and represent potential sites for formation of infective heterokaryons following mating of haploid basidiospore germlings. Use of spore‐sized micro‐beads showed that basidiospores could be pulled up to 10 cm into severed xylem vessels, where they are relatively protected from dehydration, UV irradiation and competing microflora. Diversity of isolates from five locations on two plantations was assessed by RAMS fingerprinting. Isolates from within individual palms with USR were identical and represent single infections, but different USR infections had unique band patterns and revealed separate infections. Some BSR‐affected trees contained more than one isolate, and thus had multiple infections. There was one example of adjacent BSR palms with the same isolate, indicating vegetative spread, but there were no identical genets from BSR infections and adjacent fallen palms. Isolate diversity was as great within a plantation as between plantations. It is evident that basidiospores play a major role in spread and genetic variability of G. boninense. Evidence for direct basidiospore infection via cut fronds, indirectly through roots via colonized debris and less frequently, infection by vegetative, clonal spread is considered.  相似文献   
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