Distribution of prion protein genotypes in breeds of sheep in New Zealand     

MA Lee  TR Manley  BC Glass  RM Anderson  SJ Wilson  JS O'Keefe 《New Zealand veterinary journal》2013,61(5):222-227

AIM: To use an established high through-put genotyping procedure to gain an estimate of the frequency of alleles of the prion protein (PrP) gene in some common sheep breeds in New Zealand.

METHODS: Using a genotyping procedure based on matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF), DNA samples from 3,024 sheep from New Zealand, including breeds such as Romney, Texel, Coopworth, Merino and mixedbreed, were isolated, genotyped and the results analysed.

RESULTS: The 15 scrapie genotypes commonly reported, and derived from the five commonly reported allelic variants (ARR, ARQ, AHQ, ARH and VRQ), were all observed in the samples analysed. The estimates were indicative of the frequencies in the population of alleles present in breeds of sheep in New Zealand. There was a significant difference between the frequencies of alleles between breeds, but the ARQ, followed by the ARR allele, were, except in Carwell sheep, the most common alleles present.

CONCLUSION: This study gave an indication of the percentages of PrP gene alleles in sheep in New Zealand, including data previously unreported from breeds in this country. It is of interest because of the relatively large size of the sheep population in New Zealand compared with many countries, and it provides some useful information on the genetic susceptibility or resistance of the sheep population in New Zealand to scrapie. The frequencies of the alleles can be different for an individual breed compared between countries.  相似文献   

Recombinant expression of porcine spermadhesin AWN and its phospholipid interaction: Indication for a novel lipid binding property          下载免费PDF全文

F Schröter  K Müller  P Müller  E Krause  BC Braun 《Reproduction in domestic animals》2017,52(4):585-595

AWN is a porcine (Sus scrofa domestica) seminal plasma protein and has been linked to a variety of processes related to fertilization. To acquire the protein in sufficient amount and purity for functional studies, we established its recombinant expression in E. coli and a three‐step purification protocol based on different chromatographies. The test for AWN–phospholipid interaction revealed phosphatidic acid and cardiolipin as potential binding partners. As phosphatidic acid is surmised to play a role in cation‐induced membrane destabilization and fusion events, we propose a membrane protective function of the presented binding affinity. Further studies with recombinant AWN will allow new insights into the mechanism of sperm–spermadhesin interaction and might provide new approaches for artificial reproduction techniques.  相似文献   

Effect of oral exposure to the acaricide pirimicarb,a new varroacide candidate,on Apis mellifera feeding rate          下载免费PDF全文

Clémence Riva  Michel BC Sokolowski  Julien Normand  Jana Sopkova‐de Oliveira Santos  Marie‐Pierre Halm‐Lemeille 《Pest management science》2018,74(8):1790-1797

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Treatment of coli dysentery in suckling pigs using Amfurifer     

H Bugnowski  V Hesse 《Monatshefte für Veterin?rmedizin》1970,25(24):950-954

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Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland     

Tazumi A  Maeda Y  Buckley T  Millar B  Goldsmith C  Dooley J  Elborn J  Matsuda M  Moore J 《Irish veterinary journal》2009,62(7):456-459

Clinical isolates (n = 63) of Pseudomonas aeruginosa obtained from various sites in 63 horses were compared using ERIC2 RAPD PCR to determine their genetic relatedness. Resulting banding patterns (n = 24 genotypes) showed a high degree of genetic heterogeneity amongst all isolates examined, indicating a relative non-clonal relationship between isolates from these patients, employing this genotyping technique. This study characterised 63 clinical isolates into 24 distinct genotypes, with the largest cluster (genotype E) accounting for 10/63 (15.9%) of the isolates. ERIC2 RAPD PCR proved to be a highly discriminatory molecular typing tool of P. aeruginosa in isolates recovered from horses. With the adoption of several controls to aid reproducibility, this technique may be useful as an alternative to PFGE, particularly in epidemiological investigations of outbreaks where speed may be a significant parameter. This is the first report of clonal heterogeneity amongst P. aeruginosa from horses and demonstrated that ERIC RAPD PCR is a rapid method for the examination of this species in horses, which may be useful in outbreak analysis.  相似文献   

Irrigation agriculture and the sedimentary record in the Palpa Valley,southern Peru     

Ralf Hesse  Jussi Baade 《CATENA》2009

Irrigation agriculture is an ancient and widespread feature in regions where allogenic rivers flow through arid landscapes. This paper reports the results of sedimentological–pedological investigations in the Palpa Valley in the coastal desert of southern Peru where sediment-laden river water is used for irrigation. An adapted facies classification is used to deduce a generalised stratigraphical sequence of the valley bottom sediments. Chronological constraints from radiocarbon dating and archaeological finds are used to reconstruct sedimentation rates for fossil to recent irragric anthrosols. Actualistic case studies are used to quantify present-day irrigation sedimentation. The investigations result in a clarification of the sedimentological and geomorphic history of this valley and a quantification of irrigation-related sedimentation. Irragric anthrosols date back more than 3500 years and provide direct evidence for the beginning of irrigation agriculture in this region. It is concluded that: (i) the up to approximately 4 m thick fine-grained sediments in the Palpa Valley bottom totaling a volume of 21.4 Mio. m³ are a result of long-lasting human impact and have to be considered as artefacts of prehistoric and historic irrigation agriculture in the hyperarid coastal desert; (ii) the fine-grained sediments represent a geoarchive which holds information on the history of irrigation agriculture as well as information on possible natural disturbance by extreme fluvial events; (iii) the identification of irragric anthrosols has implications for archaeological and paleoenvironmental reconstructions.  相似文献   

Canine cystine urolithiasis: A review of 1760 submissions over 35 years (1979–2013)     

Albrecht Hesse  Jenni Hoffmann  Helmut Orzekowsky  Reto Neiger 《The Canadian veterinary journal. La revue veterinaire canadienne》2016,57(3):277-281

This study reports a retrospective evaluation of epidemiological data from cystine stones of dogs submitted to the Urinary Stone Analysis Center Bonn, Germany, over a period of 35 years. Of the 20 316 uroliths submitted from 1979 to 2013, 1760 were cystine stones. In total, 109 breeds were affected with 16 breeds having an odds ratio > 1.0. Most of the cystine uroliths were retrieved from male dogs, with only 19 female dogs (1.1%) being affected. Percentage of submitted cystine stones amongst all stones decreased significantly over 35 years from 38.9% to 4.4%.  相似文献   

Filled Skutterudite Antimonides: A New Class of Thermoelectric Materials   总被引：2，自引：0，他引：2  

BC Sales  D Mandrus  RK Williams 《Science (New York, N.Y.)》1996,272(5266):1325-1328

A class of thermoelectric materials has been synthesized with a thermoelectric figure of merit ZT (where T is temperature and Z is a function of thermopower, electrical resistivity, and thermal conductivity) near 1 at 800 kelvin. Although these materials have not been optimized, this value is comparable to the best ZT values obtained for any previously studied thermoelectric material. Calculations indicate that the optimized material should have ZT values of 1.4. These ternary semiconductors have the general formula RM4X12 (where R is lanthanum, cerium, praseodymium, neodymium, or europium; M is iron, ruthenium, or osmium; and X is phosphorus, arsenic, or antimony) and represent a new approach to creating improved thermoelectric materials. Several alloys in the composition range CeFe4-xCoxSb12 or LaFe4-xCoxSb12 (0 < x < 4) have large values of ZT.  相似文献   

Uber das Vorkommen von Calciumoxalat-Kristallen1 in Heterobasidion annosum (Fr.) Bref.     

C. Von Volger  Chr. Hesse  A. Vogt 《Forest Pathology》1982,12(1):59-70

The increasing appearance of rods (»Stäbchen«) being found in Heterobasidion annosum cultures was investigated. They proved to be neither bacterial impurities nor fomannosin but calcium oxalates. The number of rod-shaped crystals depended on the quality of the tap water taken for the culture medium and on genetical aspects. The importance of calciumoxalates being formed by H. annosum is discussed.  相似文献   

Characterization of Transgenic Pigs That Express Human Decay Accelerating Factor and Cell Membrane‐tethered Human Tissue Factor Pathway Inhibitor     

HJ Lee  BC Lee  YH Kim  NW Paik  HM Rho 《Reproduction in domestic animals》2011,46(2):325-332

The expression of human complement regulatory proteins (hCRP; hDAF, hCD59, and hMCP) in pig tissues has been suggested as one of strategies to overcome the hyperacute rejection (HAR) in pig‐to‐human transplantation. Expression of human tissue factor pathway inhibitor (hTFPI) in porcine endothelial cells has been suggested as a remedy to overcome microvascular thrombosis. To investigate the effects of these combined transgenes, we established transformed pig cells expressing human decay accelerating factor (hDAF) under the control of enhancer promoter (5′LTR‐PCMVIE), and the fusion protein (hTFPI/hCD4) consisting of the functional domains (K1 and K2) of hTFPI and membrane‐tethering domains (D3 and D4) of hCD4 under the control of PCMVIE. Transgenic pigs were generated with the transformed porcine cells through somatic cell nuclear transfer (SCNT) technology. Analysis of quantitative PCR and real‐time quantitative RT‐PCR showed that four copies of hDAF were integrated and 391 copies of hDAF mRNA expressed in the cells of the transgenic pig. The enhancing activity of 5′LTR was approximately 2 fold compared to CMVIE promoter only. The cell viability test showed that more than 80% of ear cells were viable in the presence of 50% human serum. The chromogenic substrate assay and immunocytochemical staining with tail cells showed that the TFPI activity of fusion protein was observed on the cell membrane. The membrane localization of hDAF and hTFPI proteins was observed by immunocytochemical staining, and the expression of transgenes in heart and liver tissues was also confirmed by immunohistochemistry.  相似文献