Buffalo (Bubalus bubali) Late Embryo and Foetus Development: A Morphological Analysis          下载免费PDF全文

AC Morini  NCG Pieri  KCS Roballo  DS Martins  CE Ambrósio  JC Morini Junior  PO Favaron  AHH Minervino  FVT Pereira  MA Miglino 《Reproduction in domestic animals》2016,51(4):509-514

Many researches describe the embryonic developmental features in domestic animals; however, in farm animals, they are scarce. Most farm animal studies are related to assisted reproduction and embryos transfer techniques. But, morphological features and size measure to estimate the age gestation are rarely reported in literature. Thus, in this study, we described the developmental changes in the bubaline (Bubalus bubali) concepts from 21 to 60 days of gestation. Our results revealed that buffalo embryos similar morphological characteristics similar to other mammalian species. Also, similarities between bovine and bubaline persist; except on foetal stages when buffalos have a faster development than bovine. Therefore, buffalo's gestation period exhibits some varieties and accurate embryo age is more difficult. Yet, when we use a combination of the crown–rump, macroscopic analysis and alizarin red, it is possible to describe better the whole embryogenesis stages of the buffalo and which can contribute for future reproduction researches and applications in veterinary practice.  相似文献   

Isolation of verocytotoxin-producing Escherichia coli from mutton carcases     

H. SIDJABAT-TAMBUNAN  JC BENSINK  KA BETTELHEIM 《Australian veterinary journal》1998,76(5):364-365

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Breeding Soundness Evaluation and Semen Analysis for Predicting Bull Fertility     

JP Kastelic  JC Thundathil 《Reproduction in domestic animals》2008,43(S2):368-373

Bull fertility is influenced by numerous factors. Although 20–40% of bulls in an unselected population may have reduced fertility, few are completely sterile. Breeding soundness refers to a bull's ability to get cows pregnant. A standard breeding soundness evaluation identifies bulls with substantial deficits in fertility, but does not consistently identify sub-fertile bulls. In this regard, the use of frozen-thawed semen (from bulls in commercial AI centres) that meets minimum quality standards can result in pregnancy rates that differ by 20–25 percentage points. Although no single diagnostic test can accurately predict variations in fertility among bulls that are producing apparently normal semen, recent studies suggested that a combination of laboratory tests were predictive of fertility. This review is focused on recent developments in prediction of bull fertility, based on assessments at the molecular, cellular and whole-animal levels.  相似文献   

Cell Cycle Stage Analysis of Rabbit Foetal Fibroblasts and Cumulus Cells     

CT Liu  KC Yu  JC Ju 《Reproduction in domestic animals》2004,39(6):385-390

Synchronization of the cell cycle stages in G0/G1 phase is one of the key factors determining the success of nuclear transplantation. Serum deprivation, contact inhibition and chemical inhibitors are widely used methods for this purpose. In this study, cell cycle stages of foetal fibroblasts and cumulus cells were determined using flow cytometry [fluorescence-activated cell scan (FACS)]. Foetal fibroblasts (in vitro cultured for 72-120 h) and fresh cumulus cells were analysed in Experiment 1. Fifty to 55% proliferating fibroblasts remained in G0/G1 phase compared with 78% in confluent culture (p <0.05). In contrast to foetal fibroblasts, fresh cumulus cells maintained 90% of the population in the G0/G1 stage. When serum was retrieved from the proliferating fibroblasts from day 1 to day 5 (Experiment 2), proportions of G0/G1 cells increased from the initial ratio of 53 to 87% at day 4 of starvation, which was significantly higher than the non-starved proliferating cells (p <0.05). In Experiment 3, fibroblasts were treated with aphidicolin (0.1 microg/ml, 6 h), demicolcine (0.5 microg/ml, 10 h), or a combination of these two chemicals to synchronize the cell cycle stages. Surprisingly, no differences or significantly lower in the proportions of G0/G1)phase cells were detected (25-50%) compared with the uncontrolled growing cells (53%). These results suggested that fresh cumulus cells rest their cell cycle in G0/G1 stage. Serum deprivation became effective in the first 24 h and reached the highest proportions during days 4-5 after deprivation. Chemical synchronization of the cell cycle stage of rabbit foetal fibroblasts to G0/G1 phase appeared less effective compared to serum deprivation.  相似文献   

REPLY     

JC McNAE  SJ LEWIS 《Australian veterinary journal》2004,82(8):478-478

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Resistance to synthetic pyrethroid pour-on insecticides in strains of the sheep body louse Bovicola (Damalinia) ovis     

PW JOHNSON  JC BORAY  KL DAWSON 《Australian veterinary journal》1992,69(9):213-217

Groups of sheep infested with strains of Bovicola (Damalinia) ovis were obtained from flocks either with a history of failure to control lice with synthetic pyrethroid (SP) pour-on insecticides, or from farms where SP compounds were not used. The sheep were treated according to the manufacturer's recommendations with registered "off-shears" SP formulations. All treatments were applied under ideal conditions with doses calculated on an individual body weight basis and applied to the dorsal mid-line from the base of the neck to the butt of the tail. Treated sheep were kept in pens and maintained in separate groups. The pour-on SP treatments significantly reduced the lice population but failed to eliminate the infestation in 7 of 13 experiments in sheep carrying strains of lice with resistance factors of greater than 4 to at least one of the SP compounds. Failures occurred with all three of the SP pour-ons currently registered for lice control in NSW and with both water-based and organic solvent-based formulations.  相似文献   

Pre‐planting weed detection based on ground field spectral data     

Luan P Pott  Telmo JC Amado  Raí A Schwalbert  Elodio Sebem  Mithila Jugulam  Ignacio A Ciampitti 《Pest management science》2020,76(3):1173-1182

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Modular hybrid total hip arthroplasty. Experimental study in dogs     

Bruno W Minto  Cláudia Valéria S Brand?o  Gilberto JC Pereira  Daniela Campagnol  Maria Jaqueline Mamprim  Carlos Roberto Padovani  José JT Ranzani 《Acta veterinaria Scandinavica》2011,53(1):46

Background

This prospective experimental study evaluated the surgical procedure and results of modular hybrid total hip arthroplasty in dogs.

Methods

Ten skeletally mature healthy mongrel dogs with weights varying between 19 and 27 kg were used. Cemented modular femoral stems and uncemented porous-coated acetabular cups were employed. Clinical and radiographic evaluations were performed before surgery and at 30, 60, 90, 120, 180 and 360 days post-operation.

Results

Excellent weight bearing was noticed in the operated limb in seven dogs. Dislocation followed by loosening of the prosthesis was noticed in two dogs, which were therefore properly treated with a femoral head osteotomy. Femoral fracture occurred in one dog, which was promptly treated with full implant removal and femoral osteosynthesis.

Conclusions

The canine modular hybrid total hip arthroplasty provided excellent functionality of the operated limb.  相似文献   

Automated identification of intensive animal production locations from aerial photography          下载免费PDF全文

KJ Sheffield  JC Hunnam  TN Cuzner  EM Morse‐McNabb  SM Sloan  J Nunan  J Smith  W Harvey  H Lewis 《Australian veterinary journal》2018,96(9):323-331

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A finite element model of the bovine claw under static load for evaluation of different flooring conditions     

C Hinterhofer  JC Ferguson  V Apprich  H Haider  C Stanek 《New Zealand veterinary journal》2013,61(3):165-170

AIM: To create a computer-based finite element (FE) model of the bovine claw and to use finite element analysis (FEA) to estimate stress and deformation of a physiologically-shaped model claw under static load, to visualise potential material weakness and to evaluate the effect of different flooring conditions.

METHODS: Model geometry was derived using digitalised images from a recently trimmed, sound, hind claw from a 4-year-old Austrian Fleckvieh cow. Material properties of bovine claw horn were defined from preliminary investigations and recently established material data, using a modulus of elasticity from 200 to 600 N/mm² . Meshing of the model was performed with 42,127 elements based on 116,141 nodes. Loading of the model was defined at 756 N per claw on a hard and soft surface.

RESULTS: The FE model of the bovine claw under a load of 756 N showed only minimal deformation, most of which took place at the axial wall. Highest stresses were evident in the proximal axial wall, the outer edge of the weight-bearing surface and under the heels. The claw-floor contact image showed a pressure distribution resembling the distal rim of the claw wall. On the hard surface, the maximum stresses were three times higher than those on the soft surface.

CONCLUSIONS AND CLINICAL RELEVANCE: FEA allowed visualisation of the effects that loading on different floor surfaces have on the biomechanics of the claw. Uneven preparation of the claw sole resulted in high stresses at and close to irregularities of the sole. Consequences were more severe on harder flooring. The model supports the hypothesis that mechanical factors play a substantial role in the pathogenesis of claw lesions.  相似文献