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酶联免疫吸附测定法,灵敏度高,使用抗血清仅微量,是近年来用于诊断、鉴定植物病毒的先进方法之一。这一技术需要纯度高的抗血清,采用蛋白质A-琼脂糖CL-4B柱层析纯化后的抗烟草环斑病毒抗血清(抗TRSV-IgG),纯度高、灵敏,很适用于塑珠酶联免疫吸附测定法(Bead-ELISA)的抗体和酶标抗体的材料。 相似文献
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Effects of ractopamine on genetically obese and lean pigs 总被引:2,自引:0,他引:2
Twenty-eight genetically obese and 24 lean barrows (65.0 and 68.7 kg average BW, respectively) were allotted within genotype to a 16% CP corn-soybean meal basal diet or this basal diet + 20 ppm ractopamine (a phenethanolamine beta-adrenergic agonist) and allowed ad libitum access to feed for 48 d. Compared to lean pigs, obese pigs had lower ADG, gain to feed ratio, longissimus muscle area, predicted amount of muscle, and weights of trimmed loin and ham, ham lean, heart, spleen, kidney and gastrointestinal tract (P less than .05). Obese pigs also had shorter carcass but higher dressing percentage, backfat thickness, fat depth, fat area, untrimmed loin weight and fasting plasma urea N concentration (P less than .05). Dietary supplementation with 20 ppm ractopamine reduced daily feed intake and improved gain to feed ratio in both lean and obese pigs (P less than .05). Pigs fed ractopamine had shorter carcasses, less fat depth and fat area, smaller weights of stomach and colon plus rectum, but higher dressing percentages, longissimus muscle areas, weights of trimmed Boston butts, picnics and loins, ham lean and predicted amounts of muscle than pigs not fed ractopamine (P less than .05). Supplemental ractopamine had no effect on fasting plasma concentrations of urea N, nonesterified fatty acids, triglyceride or glucose (P greater than .05). No genotype x ractopamine interactions for the criteria described above were detected (P greater than .05). These results suggest that ractopamine will improve the efficiency of feed utilization and carcass leanness in swine with different propensities for body fat deposition. 相似文献
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S A Voelkel S D Viker C A Johnson K G Hill P E Humes R A Godke 《The Veterinary record》1985,117(20):528-530
A non-surgical embryo collection was completed on a day 7 superovulated Chianina donor cow. Because all but two of the ova from the collection were unfertilised and a surplus of potential recipients was available, one embryo (an excellent quality late morula) was dissected into four equal portioned 'quarter' embryos using a simplified micromanipulation procedure. Each quarter embryo was then placed in a 0.25 ml French straw and non-surgically transplanted to four different crossbred beef recipient females. The remaining embryo was similarly transplanted to a herd mate recipient as an intact embryo. One recipient returned to oestrus, one recipient had an extended post transfer cycle and the two remaining recipients produced a live quarter embryo transplant calf each within 24 hours of the other. The intact embryo placed in a herd mate recipient did not produce a transplant calf. To the authors' knowledge, these transplant offspring are the first live births reported from a non-surgically collected later-stage bovine morula (day 7), which had been dissected into quarters and then individually transplanted non-surgically to recipient females. The procedure was relatively simple to perform and was completed in less than one hour. 相似文献
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A crop yield and soil water management simulation model (CRPSM) developed at Utah State University was modified, calibrated and tested using local weather data and field results from a trickle irrigation experiment with different mulching on watermelon ( Citrullus lanatus ), carried out at the University of Jordan Research Station, in the Jordan Valley.
Simulated irrigation schedules were then applied with some of the four options provided by the model. The water yield index, WYI, introduced by B attikhi and H ill (1985) to select the most efficient schedule as based on yield and water use efficiency, was then determined. WYI ranged from 27 to 87. The field schedule, WM2 , had a WYI of 62. Whereas, the model provided a much better schedule, WM7 (WYI = 86). WM7 requires 17 irrigations of 2.0 cm per irrigation totaling a water supply of 44.1 cm with an irrigation season starting on April 7 to give a yield equivalent to the potential yield, 80.0 MT/ha. On the other hand, the best field schedule, WM2 under transparent mulch, required 14 irrigations to provide 45.9 cm (including rainfall and soil moisture change), with a season starting on April 28 resulted in a yield of 68.8 MT/ha. So we can see that by using the same amounts of total water supply but with different schedule we can get the potential yield. The model has, therefore, provided few better schedules that can be tested in the field at lower costs before final recommendations are made. 相似文献
Simulated irrigation schedules were then applied with some of the four options provided by the model. The water yield index, WYI, introduced by B attikhi and H ill (1985) to select the most efficient schedule as based on yield and water use efficiency, was then determined. WYI ranged from 27 to 87. The field schedule, WM
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Honey bees are found associated with forests globally. Flowers of forest trees provide subsistence for honey bees and the trees physically provide shelter for a swarm or bee hive. Forest management and beekeeping have each had a long history both in the United States and globally, but have seldom been integrated or studied in a systematic fashion. Purposeful plantings of trees, as in agroforestry systems, could be designed to favor bee forage or hive protection.Tree growing and beekeeping can easily be combined for several reasons. Both are sustainable on land that is hilly or otherwise less desirable for other agricultural purposes. Both require labor intermittently, and can be sustained while the grower/beekeeper is busy with other farming occupations. Bee hives require very little space, while the bees themselves can forage in a radius of 4 to 5 km. Hives may be located within or near a tree plantation, and utilize both the trees and surrounding other flowering plants for forage. Combining forestry and beekeeping provides annual honey bee products (e.g. honey, beeswax) to supplement income from a landowner's long term forest managements. In areas like Kentucky, where more than half of the forest landowners own 4 ha or less of commercially valuable woodland, it is important to produce economic benefit from those lands without harvesting all the trees. Combining bees and trees is one way of accomplishing this goal. This paper addresses several important known bee-tree interactions which need more systematic study. 相似文献