Isoflavone levels in five soybean (Glycine max) genotypes are altered by phytochrome-mediated light treatments     

Kirakosyan A  Kaufman P  Nelson RL  Kasperbauer MJ  Duke JA  Seymour E  Chang SC  Warber S  Bolling S 《Journal of agricultural and food chemistry》2006,54(1):54-58

The objective of the present study was to determine whether concentrations of different isoflavones (puerarin, genistein, genistin, daidzein, and daidzin) in shoots and roots of five selected soybean genotypes would respond the same or differently to red (650 nm peak transmittance) and far-red (750 nm peak transmittance) light treatments given under controlled environments. Levels of isoflavones (mg g(-1) dry weight biomass) present in seeds, control roots, and shoots and 10 day light-treated seedlings (light, dark, red, and far-red wavelengths) of soybean (Glycine max) were determined by high-performance liquid chromatography analysis in comparison with known isoflavone standards. Seeds of the five soybean genotypes studied consistently stored most of their isoflavones as glucosyl conjugates (e.g., daidzin, genistin, and puerarin). For the five soybean genotypes, isoflavone levels were lower in the seeds as compared with roots plus shoots of control, time zero (first true leaf stage) seedlings. Following 10 days of the respective light treatments, we found that (i) isoflavone levels were enhanced in dark-grown plants over light-grown plants for three of the five genotypes (a new finding) and the reverse occurred for a single genotype (a typical response of legumes) and (ii) generally, far-red end of day (EOD) light treatment enhanced total isoflavone levels in roots plus shoots over red EOD light treatment. Results from the present study show that phytochrome does appear to play a role in regulating isoflavone levels in developing soybean seedlings and that this influence by red/far-red-mediated phytochrome reactions is strongly dependent on the genotypes selected for study.  相似文献   

Dietary l-tryptophan alters aggression in juvenile matrinxã Brycon amazonicus     

Wolkers CP  Serra M  Hoshiba MA  Urbinati EC 《Fish physiology and biochemistry》2012,38(3):819-827

This study evaluated the effect of dietary supplementation with l-tryptophan (L-TRP), a serotonin precursor, on the aggressiveness of juvenile matrinxã Brycon amazonicus. Fish were kept in individual aquaria for 7 days receiving the diets: D1 (control: 0.47% of TRP), D2 (0.94% of TRP), D3 (1.88% of TRP), and D4 (3.76% of TRP). After this, they were grouped with an intruder fish to establish a resident–intruder relationship during periods of 20 min. Blood cortisol, glucose, chloride, sodium and calcium; hemoglobin, hematocrit, red blood cell count and volume; liver glycogen and lipids were measured. Territoriality had significant effect on the aggressiveness of matrinxã (the residents were more aggressive than intruders, P < 0.001) and tryptophan significantly affected their behavior. Fish fed with the D2 diet presented a longer latency until the first attack (P = 0.0069) and bit the intruder fewer times (P = 0.0136) during the period of observation, compared to the control group. The frequency of bites and chases after the first attack was not affected by the dietary supplementation of TRP. Physiological variables were not significantly affected by the diet, except for a moderate increase in cortisol level in fish fed with D2 diet after the fight, indicating slight activation of the hypothalamus–pituitary–interrenal axis. The results show that juvenile matrinxã have aggressive and territorial behavior and that a diet containing 9.4 g TRP kg^?1 alter their aggressiveness, without affecting the stress-related physiological parameters.  相似文献   

Evaluating the potential for weed seed dispersal based on waterfowl consumption and seed viability          下载免费PDF全文

Jaime A Farmer  Elisabeth B Webb  Robert A Pierce II  Kevin W Bradley 《Pest management science》2017,73(12):2592-2603

  相似文献   

Efficacy of an oral live vaccine for veterinary use against pseudotuberculosis     

Benoît Quintard  Thierry Petit  Nathalie Ruvoen  Elisabeth Carniel  Christian E. Demeure   《Comparative immunology, microbiology and infectious diseases》2010,33(6):e59-e65

Pseudotuberculosis, an infection caused by the ubiquitous enteropathogenic bacterium Yersinia pseudotuberculosis, is a recurrent veterinary problem in livestock and zoo animals. The only vaccine currently available in zoos is Pseudovac (a mixture of killed strains of various serotypes), but its efficacy is not well established. We show here that Pseudovac does not protect guinea pigs against a severe Y. pseudotuberculosis infection. We thus evaluated the possibility of using a live attenuated Y. pseudotuberculosis strain (IP32680) as an oral vaccine against animal pseudotuberculosis. We report that IP32680 is avirulent for guinea pigs and induces a strong IgG response against various serotypes of Y. pseudotuberculosis. One and two oral inoculations of IP32680 provided 50% and 83% protection, respectively against a severe infection with a highly pathogenic strain. The avirulent Y. pseudotuberculosis IP32680 is therefore much more protective than Pseudovac and may represent a valuable oral vaccine against pseudotuberculosis in zoo animals.  相似文献   

Progesterone intoxication inducing marked sedation in a cat     

Marc P. Dhumeaux  Elisabeth C.R. Snead  Germaine C. Hung  Susan M. Taylor 《Journal of Feline Medicine and Surgery》2010,12(10):811-813

  相似文献   

Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in <Emphasis Type="Italic">Pisum sativum</Emphasis>     

Mette Grønlund  Anne Olsen  Elisabeth I Johansen  Iver Jakobsen 《Plant methods》2010,6(1):28

Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants, which are difficult to transform. The pea early-browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. However, the available PEBV-VIGS protocols are inadequate for studying genes involved in the symbiosis with arbuscular mycorrhizal fungi (AMF).  相似文献   

Investigations of barley stripe mosaic virus as a gene silencing vector in barley roots and in <Emphasis Type="Italic">Brachypodium distachyon</Emphasis> and oat     

Andrzej Pacak  Katrin Geisler  Bodil Jørgensen  Maria Barciszewska-Pacak  Lena Nilsson  Tom Hamborg Nielsen  Elisabeth Johansen  Mette Grønlund  Iver Jakobsen  Merete Albrechtsen 《Plant methods》2010,6(1):26

Background  

Gene silencing vectors based on Barley stripe mosaic virus (BSMV) are used extensively in cereals to study gene function, but nearly all studies have been limited to genes expressed in leaves of barley and wheat. However since many important aspects of plant biology are based on root-expressed genes we wanted to explore the potential of BSMV for silencing genes in root tissues. Furthermore, the newly completed genome sequence of the emerging cereal model species Brachypodium distachyon as well as the increasing amount of EST sequence information available for oat (Avena species) have created a need for tools to study gene function in these species.  相似文献   

Inter-laboratory comparison of four RT-PCR based methods for the generic detection of pospiviroids in tomato leaves and seeds     

Thibaut Olivier  Vaidevutis Šveikauskas  Elisabeth Demonty  Kris De Jonghe  Pascal Gentit  Mojca Viršček-Marn  Sabine Grausgruber-Gröger  Sébastien Morio  Francesco Faggioli  Michèle Visage  Frédéric Fauche  Maria Gusina  Marta Luigi  Helena Lasner  Irena Mavrič-Pleško 《European journal of plant pathology / European Foundation for Plant Pathology》2016,144(3):645-654

  相似文献   

Zinc exposure has differential effects on uptake and metabolism of sulfur and nitrogen in Chinese cabbage          下载免费PDF全文

C. Elisabeth E. Stuiver  Freek S. Posthumus  Saroj Parmar  Muhammad Shahbaz  Malcolm J. Hawkesford  Luit J. De Kok 《植物养料与土壤学杂志》2014,177(5):748-757

Zinc (Zn) is a plant nutrient; however, at elevated levels it rapidly becomes phytotoxic. In order to obtain insight into the physiological background of its toxicity, the impact of elevated Zn²⁺ concentrations (1 to 10 μM) in the root environment on physiological functioning of Chinese cabbage was studied. Exposure of Chinese cabbage (Brassica pekinensis) to elevated Zn²⁺ concentrations (≥ 5 μM) in the root environment resulted in leaf chlorosis and decreased biomass production. The Zn concentrations of the root and shoot increased with the Zn²⁺ concentration up to 68‐fold and 14‐fold, respectively, at 10 μM compared to the control. The concentrations of the other mineral nutrients of the shoot were hardly affected by elevated Zn²⁺ exposure, although in the root both the Cu and Fe concentrations were increased at ≥ 5 µM, whereas the Mn concentration was decreased and the Ca concentration strongly decreased at 10 µM Zn²⁺. The uptake and metabolism of sulfur and nitrogen were differentially affected at ≥ 5 µM Zn²⁺. Zn²⁺ exposure resulted in an increase of sulfate uptake and the activity of the sulfate transporters in the root, and in enhanced total sulfur concentration of the shoot, which could be ascribed partially to an accumulation of sulfate. Moreover, Zn²⁺ exposure resulted in an up to 6.5‐fold increase in water‐soluble non‐protein thiol (and cysteine) concentration of the root. However, nitrate uptake by the root and the nitrate and total nitrogen concentrations of the shoot were decreased upon Zn²⁺ exposure, demonstrating the absence of a mutual regulation of the uptake and metabolism of sulfur and nitrogen at toxic Zn levels. Evidently, elevated Zn²⁺ concentrations in the root environment did not only disturb the uptake, distribution and assimilation of sulfate, it also affected the uptake and metabolism of nitrate in Chinese cabbage.  相似文献   

Comparative growth performance of pigs immunised with a gonadotrophin releasing factor vaccine with surgically castrated pigs and entire boars raised under conventionally managed conditions     

Fuchs T  Nathues H  Koehrmann A  Andrews S  Brock F  Klein G  Beilage Eg 《Berliner und Münchener tier?rztliche Wochenschrift》2011,124(1-2):22-27

The objective of this study was to determine the effect of using a gonadotrophin releasing factor (GnRF) vaccine on growth performance of male pigs. Vaccinated pigs were compared with surgically castrated pigs and entire males under conventionally managed farm conditions. Male pigs were randomly assigned to three treatment groups: pigs surgically castrated during the first week of life (group "C", n=274), pigs immunised twice during the fattening period with a GnRF vaccine, the first when 13-14 weeks of age and the second when 20-21 weeks of age (group "V" n=280) and entire males (group "E", n=56). Average daily weight gain (ADG) was measured during the study. Additionally, feed conversion ratio (FCR) as defined by feed intake/weight gain and feed consumption was assessed during the fattening period. No difference in the ADG during the fattening period could be determined between "C" and "V". From the start of the study until slaughter at study day 160/173 the ADG in group "E" was lower (625 g/day; P) than in group "C" (681 g/day; P < 0.001) and group "V" (675 g/day; P < 0.01). The FCR during the fattening period until study day 160, three days before the first batch of pigs were slaughtered at 24-25 weeks of age, was higher in group"C" (2.72 kg feed intake/kg weight gain) compared with group "V" (2.44 kg feed intake/kg weight gain; P < 0.001) and group "E" (2.44 kg feed intake/kg weight gain; P <0.01). Pigs in group "V" performed similarly to those in group "E" until the second vaccination (day 135) after which time they showed a substantial increase in feed intake and ADG, while retaining an FCR advantage over group C.  相似文献