Salmonella flagellin enhances mucosal immunity of avian influenza vaccine in chickens     

Chaung HC  Cheng LT  Hung LH  Tsai PC  Skountzou I  Wang B  Compans RW  Lien YY 《Veterinary microbiology》2012,157(1-2):69-77

Flagellin, a bioactive Toll-like receptor (TLR) 5 ligand, may trigger the innate immunity that in turn is important for subsequent adaptive immune responses. In the present study, the adjuvant effects of the monomeric and polymeric forms of Salmonella flagellin (mFliC and pFliC, respectively) were examined in specific-pathogen free (SPF) chickens immunized intramuscularly (i.m.) or intranasally (i.n.) with formalin-inactivated avian influenza virus (AIV) H5N2 vaccines. Results showed that mFliC cooperating with the 64CpG adjuvant significantly induced influenza-specific antibody titers of plasma IgA in the i.m.-vaccinated animals. The nasal IgA levels in the i.n.-mFliC-coadministrated AIV vaccinated chickens were significantly elevated compared to levels observed in the control group (H5N2 vaccine alone). The pFliC cooperating with the 64CpG adjuvant significantly enhanced cell proliferation of splenocytes in the i.m.-vaccinated animals. TLR3 and TLR5 expressions were activated by flagellin stimulation in vitro and in vivo. These results suggest that flagellin can be used as an adjuvant in an AIV H5N2 vaccine, especially for mucosal immunity.  相似文献   

Suppression of <Emphasis Type="Italic">Sclerotinia sclerotiorum</Emphasis> by antifungal substances produced by the mycoparasite <Emphasis Type="Italic">Coniothyrium minitans</Emphasis>     

Rui Yang  Yong Chao Han  Guo Qing Li  Dao Hong Jiang  Hung Chang Huang 《European journal of plant pathology / European Foundation for Plant Pathology》2007,119(4):411-420

A study was conducted to investigate production of antifungal substances (AFS) by Coniothyrium minitans (Cm), a mycoparasite of Sclerotinia sclerotiorum (Ss), in modified Czapek-Dox (MCD) broth and potato dextrose broth (PDB), and effects of AFS of Cm on mycelial growth and germination of sclerotia and ascospores of Ss and incidence of leaf blight of oilseed rape caused by Ss. Results showed that mycelial growth of Ss was reduced by 41.6 and 84.5% on 3 day-old cultures grown on potato dextrose agar (PDA) amended with 10% (v v⁻¹) of cultural filtrates of Cm grown in MCD (MCD_cm) after incubation for 6 and 15 days, respectively, and by 2.7 and 15.7% on PDA amended with 10% (v v⁻¹) of cultural filtrates of Cm grown in PDB for 6 and 15 days, respectively. In addition to retardation of mycelial growth, morphological abnormality of Ss such as hyphal swellings and cytoplasm granulation were also observed in colonies grown on PDA amended with cultural filtrates of MCD_cm. Sclerotia of Ss soaked in the filtrates of MCD_cm for 24 h remained viable, but their ability to undergo myceliogenic germination on PDA was delayed, compared to sclerotia treated with MCD. Germination of ascospores of Ss was unaffected on PDA amended with 10% of the filtrates of MCD_cm. However, germ tubes of Ss were shortened and deformed by the formation of hyphal swellings in the treatment of MCD_cm. Treatment of leaves of oilseed rape with cultural filtrates of MCD_cm reduced incidence of leaf blight caused by Ss, compared to the controls (water or MCD). This study suggests that AFS produced by Cm plays an important role in the suppression of mycelial growth and germ-tube development of ascospores of Ss and that there is potential for using AFS of Cm to control leaf blight of oilseed rape caused by ascospores of Ss.  相似文献   

Utilization of hydrolysed potato starch by juvenile Atlantic salmon Salmo salar L., when using a restricted feeding regime     

G I. Hemre  S Y. Shiau  D F. Deng  T. Storebakken  & S S O. Hung 《Aquaculture Research》2000,31(2):207-212

A 56‐day growth trial was conducted to study the utilization of hydrolysed potato starch by juvenile Atlantic salmon Salmo salar L. using a restricted feeding regime. Two diets supplemented with either 15% or 30% hydrolysed potato starch and a control diet without carbohydrate supplementation were each fed to triplicate groups of salmon. Feeding rate varied from 1.4% to 2.0% of body weight per day, so that fish were fed similar amounts of protein and lipid. In spite of the restricted feeding used, no growth stimulation was measured as a consequence of the additional starch intake. No variation was found in protein utilization, measured as protein efficiency ratio values (PER), while feed utilization showed decreased values as starch intake increased. Plasma glucose concentrations did not vary as a consequence of increased starch intake when measured 24 h after feeding, indicating efficient regulation of plasma glucose. In fish fed the 30% starch, the plasma triacylglycerol concentration was significantly increased, which may point to de novo lipid synthesis from the high starch intake. No variation was found in plasma cholesterol or protein concentrations, or asparagine aminotransferase, alanine aminotransferase and lactate dehydrogenase activities. This shows that the fish health status and liver function were normal (no mortality was registered). Liver and muscle showed increased glycogen levels as a function of increased starch intake. The same diets were also fed to juvenile white sturgeon Acipencer transmontanus and hybrid tilapia Oreochromis niloticus×O. aureus. These results are presented separately.  相似文献   

日粮精粗比对荷斯坦青年公牛生长和肉质的影响     

梁大勇  昝林森  张双奇 《西北农林科技大学学报(自然科学版)》2009,37(12):63-67

【目的】研究不同日粮精粗比对荷斯坦青年公牛生长和肉质的影响,为荷斯坦公牛育肥肉用提供理论依据。【方法】选择9头6月龄体质量相近的荷斯坦公牛,随机分为Ⅰ、Ⅱ、Ⅲ3组,分别饲喂m(精料)∶m(粗料)为4∶6,5∶5和6∶4的日粮,粗料为苜蓿干草+玉米青贮,试验期3个月。试验结束时试验牛只全部屠宰,每头奶公牛取新鲜背最长肌2 kg分装于冰盒带回,置于洁净保鲜袋中密封冷冻保存。对肉样肉质性状、水分、粗灰分、粗脂肪、粗蛋白和脂肪酸含量进行测定。【结果】(1)Ⅱ组和Ⅲ组比Ⅰ组牛的9月龄体质量分别提高了13.40和17.00 kg,总增体质量分别提高了7.20和11.00 kg,平均日增体质量分别提高了0.07和0.10 kg,差异均达显著水平(P<0.05)。(2)Ⅲ组比Ⅰ组的嫩度低0.34、失水率低1.68%、系水力高2.79%、粗脂肪含量高0.66%,差异均达显著水平(P<0.05)。(3)Ⅲ组的饱和脂肪酸含量显著高于Ⅰ组和Ⅱ组(P<0.05),但其不饱和脂肪酸和必需脂肪酸含量均显著低于Ⅰ组和Ⅱ组(P<0.05)。【结论】提高日粮精粗比可促进荷斯坦青年公牛的生长发育,改善牛肉的肉质性状。从营养学角度看,低精料处理组(Ⅰ)和中精料处理组(Ⅱ)的牛肉品质优于高精料处理组(Ⅲ)。  相似文献   

Renal extramedullary plasmacytoma in a dog     

Jason Johnson  Germaine Hung  Victoria Larson  Jorden Manasse  Tim Spotswood 《The Canadian veterinary journal. La revue veterinaire canadienne》2021,62(10):1077

A 10-year-old, intact male Siberian husky dog was presented for a suspected left renal cyst. Computed tomography (CT) identified a large, left kidney mass with retroperitoneal hemorrhage. A left-sided nephrectomy was performed, and histopathology confirmed a renal plasmacytoma. Perioperative screening for multiple myeloma was negative. The dog was lost to follow-up and was euthanized 11 months after surgery. A necropsy was not performed. To the authors’ knowledge, this is the first case of renal extramedullary plasmacytoma in a dog.Key clinical message:This report describes the clinical presentation, and laboratory, diagnostic imaging, and surgery findings of a case of renal extramedullary plasmacytoma in a dog.  相似文献   

A comparison of the effect of dietary fungal phytase and dicalcium phosphate supplementation on growth performances,feed and phosphorus utilization of tra catfish juveniles (Pangasianodon hypophthalmus Sauvage, 1878)          下载免费PDF全文

L.T. Hung  N.T. Thanh  M.A. Pham  C.L. Browdy 《Aquaculture Nutrition》2015,21(1):10-17

Six isonitrogenous (320 g kg^?1) and isolipidic (60 g kg^?1) diets were formulated with graded levels (0, 5, 10 and 15 g kg^?1) of dicalcium phosphate (DCP) and fungal phytase (750 and 1500 FTU kg^?1 diet). Tra catfish (Pangasianodon hypophthalmus), 9.6 g, were fed the diets for 12 weeks. Each experimental diet was fed to eight replicates of fish to apparent satiation. At the end of the trial, fish fed the diets containing 15 g kg^?1 DCP, 750 and 1500 FTU kg^?1 phytase had higher growth performances, protein efficiency ratio and phosphorus retention than those fed the control diet, 5 g kg^?1 DCP and 10 g kg^?1 DCP diets (P < 0.05). Whole body ash and phosphorus concentration of fish fed the 10 g kg^?1 DCP and 15 g kg^?1 DCP diets were significantly higher than those of fish fed the control diet. Higher apparent digestibility coefficient of phosphorus was observed in fish fed the phytase supplemented diets. The present results indicate that supplementation of phytase at 750 FTU kg^?1 and 1500 FTU kg^?1 improves growth performances, feed and phosphorus utilization. The supplementation can completely replace dicalcium phosphate or other phosphorus sources in tra catfish feed and reduce the phosphorus discharge into environment.  相似文献   

Increase in the plant protein ratio in the diet of white shrimp,Litopenaeus vannamei (Boone), using Bacillus subtilis E20‐fermented soybean meal as a replacement          下载免费PDF全文

Ya‐Li Shiu  Saou‐Lien Wong  Wang‐Chen Guei  Yu‐Ching Shin  Chun‐Hung Liu 《Aquaculture Research》2015,46(2):382-394

This study aimed to improve the nutritional value and utilization of soybean meal (SBM) used as feed ingredient for shrimp by an approach of solid‐state fermentation with Bacillus subtilis E20. The protein content and B. subtilis E20 proliferation significantly increased, as the initial moisture increased from 30% to 50% during fermentation. Compared with SBM, the protein content of FSBM increased by 19% after fermentation, accompanied by an increase of 18.75% in the total hydrolyzed amino acids. The free amino acid profile and content in FSBM also obviously increased by 374.9% compared with SBM. The FSBM is a good substitute for fish meal (FM) in a diet with 37% protein and 7% lipid content. The maximal replacement levels of FM in shrimp diet with SBM and FSBM were 37.42% and 61.67%, respectively, based on the feed efficiency estimated by a broken‐line analysis. The B. subtilis E20‐FSBM can be a potential protein source used as a replacement for FM in shrimp diet.  相似文献   

Combining Taguchi Method with fuzzy inference on process optimization for fiber manufacturing     

Mei-Ling Huang  Yung-Hsiang Hung  Wan-Chi Kuo 《Fibers and Polymers》2015,16(12):2670-2681

Lack of natural textile resources, the present textile industry in Taiwan usually uses pre-oriented yarn (POY), kind of artificial fibers, to make yarns. The POY is wound from continuous spinning of esterification and superposition of plastic pure terephthalic acid (PTA) and ethylene glycol (EG). According to yarn assessment indicators, yarn breakage of POY is crucial. And the broken filament and toughness are the most two important indicators causing yarn breakage during quality measurement. This study applies Taguchi Method to jointly consolidate broken filament degradation rate and toughness elongation percentage to establish a proper orthogonal array. The experimental control factors includes knotting device type, winding tension (CN), oil rate (%), and knotting pressure (kg/cm²), and a L ₁₈(2¹×3⁷) orthogonal array is established. The key parameter design of control factors can be found by Taguchi experiment. The fuzzy inference is combined with Taguchi multiple quality characteristics to construct the process parameter module to effectively increase product yield.  相似文献   

Allotransplantation of Transgenic Mouse Ovaries Expressing Enhanced Green Fluorescent Protein under the Control of the Murine Phosphoglycerate Kinase 1 Promoter     

HT Wu  CK Chou  YC Hung  CK Yu 《Reproduction in domestic animals》2010,45(5):900-906

The aim of this study was to generate a transgenic mouse that ubiquitously expressed enhanced green fluorescent protein (EGFP) under the control of the murine phosphoglycerate kinase 1 promoter by allotransplantation of transgenic mouse ovaries. The EGFP transgenic mice expressed green fluorescence in many organs, and the fluorescence was detected as early as the embryonic stage. Ovaries from the EGFP transgenic mice were allotransplanted into recipients and these mice were mated with normal male mice. Histological sections of EGFP‐allotransplanted ovaries from the recipient mice showed the well development and formation at follicles and corpora lutea. The green fluorescence was clearly detectable at the allotransplanted section of the ovaries, which had fused with the normal ovary. The average size of the first litter from these mice was 6.8 ± 1.2 pups per recipient, and 17.8% of the pups expressed EGFP. These results demonstrated that allotransplantation of transgenic ovaries can restore a normal reproductive lifespan and can be used to generate a ubiquitously expressing EGFP animal model.  相似文献   

Comparison of planktonic microbial abundance and dissolved oxygen consumption between the aquaculture ponds of mudskippers and shrimps in the Mekong Delta,southern Vietnam     

Minoru Wada  Fumiaki Mori  Kazuki Yokouchi  Mitsuharu Yagi  Toru Takita  Atsushi Ishimatsu  Mitsunori Iwataki  Kazuya Takahashi  Hieu Van Mai  Toan Thanh Vo  Hung Phuoc Ha  Dinh Dac Tran 《Fisheries Science》2016,82(5):787-797

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