MAGNETIC RESONANCE IMAGING FOR THE DIAGNOSIS OF STRESS FRACTURES IN A HORSE     

Jackie  Tapprest  DVM  Fabrice  Audigie  DVM  PHD  Catherine  Radier  DM  Marie-Christine  Anglade  DM  Marie-Catherine  Voisin  DM  PHD  Nathalie  Foucher  Claire  Collobert-Laugier  DVM  Didier  Mathieu  DM  PHD  Jean-marie  Denoix  DVM  PHD 《Veterinary radiology & ultrasound》2003,44(4):438-442

In humans, magnetic resonance (MR) imaging is the method of choice for the diagnosis of stress fractures. In this paper, bilateral stress fracture of the lateral condyle of the third metacarpal bone in a French trotter is described. Results of the radiographic, MR imaging, and histologic examinations are presented, with a focus on the MR signal abnormalities found. Based on this patient, the potential use of MR imaging for the diagnosis of stress fractures in horses is discussed.  相似文献   

Nematode parasites in young cattle: what role for unexpected species?     

TS Waghorn  CLG Bouchet  K Bekelaar  DM Leathwick 《New Zealand veterinary journal》2019,67(1):40-45

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Changes in Testicular Development, Ultrasonographic and Histological Appearance of the Testis in Buck Kids Immunized Against LHRH Using Recombinant LHRH Fusion Protein     

H Ülker  M Küçük  A Y&#;lmaz  M Yörük  L Arslan  DM deAvila  JJ Reeves 《Reproduction in domestic animals》2009,44(1):37-43

This study was designed to evaluate the effectiveness of recombinant Ovalbumin-LHRL (OL) immunization on changes in testicular size, histological appearance and testosterone production in buck kids. Thirty native buck kids at 18 weeks of age were divided into three groups, control (n = 10), immunization (n = 10) and castration (n = 10) groups. Immunized animals received OL protein generated by recombinant DNA technology. Ultrasonographic and histological examinations of the testes were performed. Animals were slaughtered at 44 weeks of age. Semen and epididymides were evaluated for the presence of sperm cells. Immunized animals generated anti-LHRH antibodies. Testosterone production, testicular and accessory glands development and sperm production were suppressed in the immunized animals (p < 0.01). Semineferous tubule diameters decreased (p < 0.01), basal membrane of the tubule was thickened and hyalinized in immunized kids. Immunization affected ultrasonographic appearance of the testes drastically. While testes of control animals gained their normal ultrasonographic appearance as the age increased, immunized animals had uniform hypoechogenic testicular structure as observed at 18 weeks of age until slaughter. Simultaneous histological and ultrasonographic evaluations indicated that the changes in testicular histology could partly be monitored via ultrasonographic imaging; nevertheless, it is difficult to claim that ultrasonographic image reflects the exact changes in such instances. In conclusion, these results indicate that recombinant OL fusion protein is effective in immunocastration in buck kids and has a potential to be used as an alternative to physical castration. Further researches should be conducted to help assessing reproductive status of testes from ultrasound images.  相似文献   

Third‐compartment cannulation in alpacas using a polyurethane gastrostomy tube     

GW Smith  MP Gerard  NB Campbell  DM Foster  SM Smith  JL Davis 《Australian veterinary journal》2009,87(12):487-491

Objective To develop a simple and effective surgical technique for third‐compartment cannulation in alpacas. Design Prospective study using six adult male alpacas. Methods General anaesthesia was induced and a polyurethane gastrostomy tube was surgically implanted into the distal portion of the third compartment. Results Three of the alpacas retained their cannulas for a 100‐day period; however, three cannulas were dislodged during the study. Two of the three dislodged cannulas were replaced during a second surgical procedure. Cannulas were well tolerated by the alpacas and all animals remained clinically healthy during the study period. Third compartment contents did not leak from the cannulation site. The tubes were manually removed following the completion of the study and the small defect in the body wall quickly healed over in all animals. Conclusion Surgical placement of polyurethane tubes designed for percutaneous endoscopic gastrostomy is a useful method of cannulating the third compartment in camelids. This technique can be used for experimental studies and possibly could be used for nutritional support and fluid therapy in sick camelids that might need long‐term care.  相似文献   

Congenital dyserythropoietic anaemia and dyskeratosis in Australian Poll Hereford calves     

AE Kessell  DM Hanshaw  JW Finnie  P Nosworthy 《Australian veterinary journal》2012,90(12):499-504

Congenital dyserythropoietic anaemia (CDA) is a heterogeneous group of rare genetic disorders that in humans is characterised by ineffective haematopoiesis with morphological abnormalities in erythroid precursor cells and secondary iron overload. In the 1990s, a syndrome of CDA with dyskeratosis and progressive alopecia was reported in Poll Hereford calves in Canada and the USA. We report the clinical and pathological findings in two Poll Hereford calves with this syndrome from separate properties in South Australia. The animals had a variably severe anaemia, associated with abnormal nucleated red blood cells in peripheral blood, and large numbers of rubricytes and metarubricytes with a characteristic nuclear ultrastructure in the bone marrow. Both calves were born with a wiry hair coat and a progressively ‘dirty‐faced’ appearance associated with hyperkeratosis and dyskeratosis (apoptosis).  相似文献   

Clouds of venus: a preliminary assessment of microstructure     

Knollenberg RG  Hunten DM 《Science (New York, N.Y.)》1979,205(4401):70-74

The multimodal microstructure of the Venus cloud system has been examined. In addition to confirmed H(2)SO(4) droplets and suspected elemental sulfur, a highly concentrated aerosol population has been observed extending above, within, and below the cloud system. These aerosols appear to cycle through the cloud droplets, but can never be removed by the weak precipitation mechanisms present. All cloud particles are likely laced with aerosol contaminants. Sedimentation and decomposition of H(2)SO(4) in the droplets of the lower cloud region contribute more than 7 watts per square meter of heat flux equaling one-fourth of the solar net flux at 50 kilometers.  相似文献   

Fourier spectroscopy of planets     

Hunten DM 《Science (New York, N.Y.)》1968,162(3851):313-318

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Planetary science. Clues to the Martian atmosphere     

Hunten DM 《Science (New York, N.Y.)》2001,294(5548):1843-1844

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Genome‐Wide Association Study for Sperm Concentration in Holstein‐Friesian Bulls     

DM Hering  K Olenski  S Kaminski 《Reproduction in domestic animals》2014,49(6):1008-1014

The aim of the study was to screen the entire bull genome to identify markers and candidate genes underlying sperm concentration. The analysed data set originates from a population of 877 Polish Holstein‐Friesian bulls. Based on sperm concentration value, two extreme groups of bulls were created: Low (L, n = 126) and High (H, n = 140). Each bull was genotyped using the Illumina BovineSNP50 BeadChip. Genome‐wide association analysis was performed with the use of GoldenHelix SVS7 software. An additive model with a Cohran–Armitage test, Correlation/Trend adjusted by a Bonferroni test, was used to estimate the effect of SNP marker for sperm concentration. Thirteen markers reached genome‐wide significance. The most significant SNPs were located on chromosome 3 (rs109154964 and rs108965556), 14 (rs41621145) and 18 (rs41615539), in the close vicinity of protein arginine methyltransferase 6 (PRMT6), Sel1 repeat containing 1 (SELRC1), triple QxxK/R motif containing (TRIQK) and zinc finger homeobox 3 (ZFHX3) genes, respectively. For three other candidate genes located close to significant markers (within a distance of ca 1 Mb), namely histone deacetylase 9 (HDAC9), an inhibitor of DNA binding 2 (ID2) and glutathione S‐transferase theta 1 (GSTT1), their potential role in the production of male germ cells was confirmed in earlier studies. Six additional candidate genes (Vav3, GSTM1, CDK5, NOS3, PDP1 and GAL3ST1) were suspected of being significantly associated with sperm concentration or semen biochemistry. Our results indicate the genetic complexity of sperm concentration but also open the possibility for finding causal polymorphism useful in marker‐assisted selection.  相似文献   

Use of Cholesterol‐Loaded Cyclodextrin in Donkey Semen Cryopreservation Improves Sperm Viability but Results in Low Fertility in Mares     

RR Oliveira  DM Rates  G Pugliesi  PG Ker  RP Arruda  EA Moraes  GR Carvalho 《Reproduction in domestic animals》2014,49(5):845-850

The use of cholesterol‐loaded cyclodextrin (CLC) on semen cryopreservation has been related with better sperm viability in several species; however, the effect on fertility is not known in donkey semen. Ejaculates (n = 25) from five donkeys were diluted in S‐MEDIUM with 0, 1, 2 or 3 mg of CLC/120 × 10⁶ spermatozoa. Semen was frozen, and thawed samples were evaluated by computer‐assisted sperm analyser system (CASA), supravital test, hyposmotic swelling test and fluorescent dyes to assess the integrity of sperm membranes. Mares (n = 60) were inseminated with frozen‐thawed semen treated with the doses of 0 or 1 mg CLC. Percentages of sperm with progressive motility and with functional plasma membrane were greater (p < 0.05) in the CLC‐treated groups than in the control. Percentages of intact plasma membrane and intact plasma membrane and acrosome detected by fluorescent dyes were also greater (p < 0.05) in CLC‐treated groups. Although no difference (p > 0.05) in conception rates was detected between groups (control, 3/30, 10%; CLC‐treated, 1/30, 3.3%), fertility was low for artificial insemination programs in mares. Therefore, we firstly demonstrated that frozen semen treated with CLC in S‐MEDIA extender before freezing improves the in vitro sperm viability, but semen treated or not with CLC in S‐MEDIUM extender results in a very low conception rate in mares inseminated with thawed donkey semen.  相似文献