The protein kinase family: conserved features and deduced phylogeny of the catalytic domains   总被引：583，自引：0，他引：583  

S K Hanks  A M Quinn  T Hunter 《Science (New York, N.Y.)》1988,241(4861):42-52

In recent years, members of the protein kinase family have been discovered at an accelerated pace. Most were first described, not through the traditional biochemical approach of protein purification and enzyme assay, but as putative protein kinase amino acid sequences deduced from the nucleotide sequences of molecularly cloned genes or complementary DNAs. Phylogenetic mapping of the conserved protein kinase catalytic domains can serve as a useful first step in the functional characterization of these newly identified family members.  相似文献   

Biochemistry. Ubiquitination--more than two to tango     

Joazeiro CA  Hunter T 《Science (New York, N.Y.)》2000,289(5487):2061-2062

The ubiquitin pathway in the cell is an elegant system for targeting unwanted proteins for degradation. Three enzymes, E1, E2, and E3, are responsible for attaching the ubiquitin tag to proteins destined to be chopped up. In their Perspective, Joazeiro and Hunter discuss new structural findings that reveal the part played by an E3 called c-Cbl in this ubiquitinating process.  相似文献   

Anomalous type 17 response to viral infection by CD8+ T cells lacking T-bet and eomesodermin   总被引：1，自引：0，他引：1  

Intlekofer AM  Banerjee A  Takemoto N  Gordon SM  Dejong CS  Shin H  Hunter CA  Wherry EJ  Lindsten T  Reiner SL 《Science (New York, N.Y.)》2008,321(5887):408-411

  相似文献   

Intellectual property rights. Public sector collaboration for agricultural IP management     

Atkinson RC  Beachy RN  Conway G  Cordova FA  Fox MA  Holbrook KA  Klessig DF  McCormick RL  McPherson PM  Rawlings HR  Rapson R  Vanderhoef LN  Wiley JD  Young CE 《Science (New York, N.Y.)》2003,301(5630):174-175

  相似文献   

A FURTHER NOTE ON THE ANALYSIS OF ELECTROMYOGRAMS     

Travis LE  Hunter TA 《Science (New York, N.Y.)》1932,75(1955):639-640

  相似文献   

Evolutionary rate in the protein interaction network     

Fraser HB  Hirsh AE  Steinmetz LM  Scharfe C  Feldman MW 《Science (New York, N.Y.)》2002,296(5568):750-752

High-throughput screens have begun to reveal the protein interaction network that underpins most cellular functions in the yeast Saccharomyces cerevisiae. How the organization of this network affects the evolution of the proteins that compose it is a fundamental question in molecular evolution. We show that the connectivity of well-conserved proteins in the network is negatively correlated with their rate of evolution. Proteins with more interactors evolve more slowly not because they are more important to the organism, but because a greater proportion of the protein is directly involved in its function. At sites important for interaction between proteins, evolutionary changes may occur largely by coevolution, in which substitutions in one protein result in selection pressure for reciprocal changes in interacting partners. We confirm one predicted outcome of this process-namely, that interacting proteins evolve at similar rates.  相似文献   

Studies show value of forced air ventilation for potato storages     

James Hunter 《American Journal of Potato Research》1960,37(5):182-185

  相似文献   

The relationship between tail biting in pigs, docking procedure and other management practices   总被引：1，自引：0，他引：1  

Hunter EJ  Jones TA  Guise HJ  Penny RH  Hoste S 《Veterinary journal (London, England : 1997)》2001,161(1):72-79

The tail length (docked, tipped or undocked) and tail status (bitten or unbitten) of 27,870 pigs from 450 units was recorded at six UK abattoirs. A farm survey of the final finishing stage was used to investigate the relationship between management practice and tail biting. This showed that docking was the most important factor influencing the probability of being not bitten, with 2.4% of docked and 8.5% of long-tailed pigs being tail-bitten. The following factors reduced the probability of long-tailed pigs being tail-bitten; light straw provision, use of natural ventilation or artificially controlled natural ventilation (ACNV), mixed sex grouping, meal or liquid feeding, and use of double or multi-space feeders. Docked and long-tailed pigs provided with light straw and natural ventilation/ACNV had levels of tail biting of 1.2% and 4.3% respectively; 3.9% of docked pigs with artificial ventilation and no straw were tail-bitten. Long-tailed pigs fed via double or multi-space feeders also had 3.9% of tails bitten.  相似文献   

Measurement of canine IgE using the alpha chain of the human high affinity IgE receptor     

Stedman K  Lee K  Hunter S  Rivoire B  McCall C  Wassom D 《Veterinary immunology and immunopathology》2001,78(3-4):349-355

In vitro assays for allergen specific immunoglobulin E (IgE) are a convenient and reproducible alternative to intradermal skin testing in dogs. Such tests may be used to support a diagnosis of atopic dermatitis and to define appropriate allergens for immunotherapy. Current in vitro assays rely upon monoclonal or polyclonal antibodies as IgE detection reagents. However, in sera where allergen-specific IgG occurs in great excess, any IgE:IgG cross-reactivity of the detection reagent may result in lowered assay specificity. Therefore, we have developed an assay for canine IgE which uses a recombinant form of the extracellular part of the alpha chain of the human high affinity IgE receptor (FcvarepsilonRIalpha). Biotinylated FcvarepsilonRIalpha shows no significant binding to purified canine IgG, and recognizes a heat labile antibody in serum, with a detection limit of 73-146pg/ml. Comparison of assay signals using the labeled FcvarepsilonRIalpha and a highly specific anti-canine IgE monoclonal antibody (MAb) shows good agreement. The FcvarepsilonRIalpha is therefore a sensitive and specific alternative to polyclonal or monoclonal antibodies for canine serum IgE measurement.  相似文献