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Ultrastructural study of the liver and kidney in ochratoxicosis A in young broiler chicks 总被引:1,自引:0,他引:1
Ultrastructural changes are reported in the kidney and liver of 20-day-old broiler chicks fed ochratoxin A (OA), incorporated in the diet at levels of 2 and 4 ppm. Changes in the kidney included the presence of abnormally shaped mitochondria in the proximal convoluted tubules. There was an increase in the size and number of mitochondrial dense granules and cytoplasmic peroxisomes. Intranuclear and cytoplasmic lipid droplets and electron dense round bodies in the dilated smooth endoplasmic reticulum were also noted. Regional thickening and degeneration of the glomerular basement membrane was observed in some cases. In the liver from OA fed birds there was an increased accumulation of cytoplasmic glycogen in the hepatocytes. Abnormal mitochondrial ring forms in the kidney and the accumulation of glycogen in the liver are considered to be of diagnostic significance in ochratoxicosis of young broiler chicks. The severity of the changes was found to be dose related. These results suggest that the mitochondria in the proximal convoluted tubules of kidney were most sensitive to OA toxicity. 相似文献
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Ultrathin sections of liver and kidney from 11-week-old quail fed, from day-old, ochratoxin A (OA) (mixed in the diet at levels of 4 and 8 ppm) were examined in the electron microscope. Pathological changes in the kidneys were limited to the proximal convoluted tubules (PCT) and glomeruli. In the PCTs, abnormal mitochondria and excessive numbers of lipid droplets were the principal findings with glomeruli showing thickened basement membranes. Swollen mitochondria and variable glycogen deposition were the chief features present in the livers. It is suggested that OA is more hepatotoxic in quail than in broilers, as well as being nephrotoxic. 相似文献
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Radioimmunosorbent techniques using antisera to human immunoglobulin E (IgE) showed the presence of high levels (up to 15,840 U/ml) of IgE in serum and plásma from fowls immunised by repeated intraperitoneal injections of horse serum. These results were confirmed by passive cutaneous anaphylaxis and heat lability tests. 相似文献
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One hundred fifty Simmental-Hereford cows and calves were used in a 3-yr study to evaluate three creep grazing treatments and an early weaning treatment on cow and calf performance during midsummer (July to September). Calves were approximately 150 d of age and averaged 178.6 kg when treatments were initiated. Tifleaf pearl millet (Pennisetum Americanum L. Leeke) was used as the forage for two of the creep treatments, representing two cow stocking intensities of .466 (TLM1) and .239 (TLM2) ha of base hill land pasture/cow, and as pasture for early weaned calves. A red clover (Trifolium pratense L.)-Kentucky bluegrass (Poa pratensis L.) mixture was used as the other creep forage. Hill land pastures were similar for the mature cow units in all creep treatments. Calf average daily gains ranged from .93 to 1.10 kg and were not influenced (P greater than .05) by treatment. Calf gains per hectare were similar for the control, red clover and TLM1 treatments. The TLM2 and early weaning treatments resulted in increases of 105.4 and 39.1 kg of calf gain/ha (P less than .05) compared with the control. When calves were allowed to creep graze millet, decreasing the forage area from .466 to .239 ha per cow-calf unit resulted in an increase of 97.7 kg of calf gain/ha with no reduction in calf gain. Cows on the more intensively grazed millet creep treatment (TLM2) lost more weight (P less than .05) during midsummer than those on the TLM1 treatment, but they gained 18.5 kg more (P less than .10) weight than TLM1 cows between weaning and the start of winter feeding. 相似文献
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