Distinct Effects of Boar Seminal Plasma Fractions Exhibiting Different Protein Profiles on the Functionality of Highly Diluted Boar Spermatozoa     

EM García  JJ Calvete  L Sanz  J Roca  EA Martínez  JM Vázquez 《Reproduction in domestic animals》2009,44(2):200-205

The aim of this study was to evaluate how different protein profiles of seminal plasma (SP) fractions affect sperm functionality in vitro. Ejaculates from three boars were separated into six fractions. The fractions differed from each other in their sperm content, in their total SP protein content, and their spermadhesin PSP-I/PSP-II and heparin-binding protein (HBP) concentrations. Spermatozoa were mainly recovered in fraction 2 (sperm-rich fraction, >1800 × 10⁶ spermatozoa/ml), whereas the pre-sperm fraction 1 and the post-sperm fractions 4–6 contained low numbers of spermatozoa (<500 × 10⁶/ml). Except in fraction 2, the total SP protein concentration and the concentration of both, spermadhesin PSP-I/PSP-II and the HBPs increased with fraction order. Distinct time-dependent effects were observed on motility characteristics and membrane integrity of highly diluted boar spermatozoa upon incubation with a 10% dilution of the SP from each fraction. The highest sperm viability was recorded after exposure for 5 h to fraction 2, followed by fractions 1 and 3. The percentages of motile spermatozoa also differed significantly among fractions after 5 h of incubation. Spermatozoa incubated with SP of fractions 1–3 showed the highest percentage motility. We conclude that different SP fractions exert distinct effects on the functionality of highly diluted boar spermatozoa. Fractions 1–3 appear to promote sperm survival, whereas fractions 4–6 seem to be harmful for preserving the physiological functions of highly diluted boar spermatozoa.  相似文献   

Effect of Suppression of FSH with a GnRH Antagonist (Acyline) Before and During Follicle Deviation in the Mare     

CM Checura  MA Beg  EL Gastal  MO Gastal  MC Wiltbank  JJ Parrish  OJ Ginther 《Reproduction in domestic animals》2009,44(3):504-511

A GnRH antagonist (Acyline) was used to study the role of FSH in early development of a follicular wave in 61 mares. In Experiment 1, a single dose of 3 mg per mare, compared with 0 and 1 mg, suppressed both the FSH and follicle responses to exogenous GnRH. In Experiment 2, high concentrations of FSH were induced by two successive ablations of all follicles ≥ 6 mm on days 10 and 13 (day 0 = ovulation). A single treatment with Acyline resulted in significantly greater suppression of plasma concentrations of FSH than a single treatment with charcoal-extracted follicular fluid (source of inhibin) or oestradiol. Suppression of FSH was not significantly different between the group treated with Acyline alone and a group treated with a combination of Acyline, inhibin and oestradiol. In Experiment 3, all follicles were ablated on day 10 to induce an FSH surge and a new follicular wave. Acyline treatment on day 10 resulted in an immediate decrease in FSH, without a significant effect on day of emergence of a new wave or growth of follicles from 7 to 11 mm on days 11–13. Treatment on day 15, a day before expected follicle deviation and after the peak of the wave-stimulating FSH surge, resulted in an immediate decrease in FSH and cessation of follicle growth. Results indicated that growth of follicles for about 2 days after wave emergence was independent of FSH. In contrast, during the decline in the wave-stimulating FSH surge and before follicle deviation, growth of follicles was dependent on FSH.  相似文献   

Genomic Imprinting in Canis familiaris     

CM Nolan  FM O'Sullivan  DC Brabazon  JJ Callanan 《Reproduction in domestic animals》2009,44(S2):16-21

For the vast majority of mammalian genes, maternally- and paternally-derived alleles behave identically and are either expressed or repressed, regardless of whether they were inherited from egg or sperm. For imprinted genes, however, this is not the case. The alleles of imprinted genes are epigenetically modified in a parent-of-origin-specific manner and, as a consequence, maternally- and paternally-derived alleles behave differently. Typically one allele is expressed while the other is silent. Although relatively few in number, imprinted genes are the focus of intensive study, as they have important roles in embryonic development. Abnormal expression of imprinted genes results in growth disorders and is implicated in several clinical conditions. Most studies of imprinted genes have been performed in rodents or primates, with limited studies in other mammals such as bovine and opossum. We have recently demonstrated the existence of imprinted genes in the canine, by showing that the canine insulin-like growth factor 2 receptor gene ( IGF2R ) is monoallelically expressed, with predominant expression of the maternally-derived allele and repression of the paternally-inherited allele. Our ultimate goal is to characterize all imprinted genes in the canine, and to understand how they contribute to canine reproduction, development and disease. Such knowledge will be vital for optimizing the success of most reproductive strategies in the canine.  相似文献   

High Embryonic Recovery Rates with In vivo and Ex vivo Techniques in the Bitch     

MR Luz  CC de Holanda  JJ Pereira  PMC Freitas  AEP Salgado  J Di Giorgio Giannotti  SB de Oliveira  NS Teixeira  CR de Freitas Guaitolini 《Reproduction in domestic animals》2011,46(4):724-727

The embryonic collection techniques in dogs present a vast methodological variation and low recovery rates. The objectives were to compare and describe two techniques as to the recovery of canine embryos, on the 12th day after the first mating or artificial insemination. Embryos were recovered through uterine horn flushing in vivo, before performing the ovariohysterectomy (OHE) (Group 1; n = 9) or ex vivo, immediately after the OHE (Group 2; n = 9). In total, 43 and 47 embryonic structures were recovered in Groups 1 and 2, respectively. There was no significant difference (p > 0.05) between groups on recovery rates (72.8% and 81.0%, respectively). We inferred that both in vivo and ex vivo techniques allow a high rate of embryonic recovery; in the collection technique prior to the OHE, it is essential to carefully handle the reproductive system during the trans‐surgical period and that the 12th day (D12) after the first mating/artificial insemination is an efficient option for the high recovery rate of morulae and blastocysts.  相似文献   

A visual pigment with two physiologically active stable states   总被引：6，自引：0，他引：6  

P Hillman  S Hochstein  B Minke 《Science (New York, N.Y.)》1972,175(29):1486-1488

Red illumination of a Balanus amphitrite photoreceptor that has been adapted to blue light leads to prolonged depolarization in the late receptor potential. This depolarization can be switched off by further exposure to a blue stimulus. The early receptor potential in this cell is purely depolarizing or largely hyperpolarizing; the former is true if the cell has been adapted to red light, and the latter, if blue light has been used. The color-adaptation "memories" for both early and late receptor potentials appear to be permanent. The existence of two stable states for the early receptor potential directly implies a pigment with two stable states, and these apparently contribute antagonistically to the late receptor potential.  相似文献   

Effect of Estradiol and Progesterone on Metabolic Biomarkers in Healthy Bitches     

A Tvarijonaviciute  JD Carrillo‐Sanchez  JJ Ceron 《Reproduction in domestic animals》2013,48(3):520-524

The aim of this study was to evaluate the possible association between hormonal changes that occur during oestrus and biomarkers related with glucose metabolism (glucose and insulin), lipid metabolism (lipidic profile and BChE) and adipokines (adiponectin and ghrelin) in healthy bitches. For this purpose, we measured these analytes in serum of bitches, at two times: before (T1) and after (T2) the LH peak that were established according to progesterone concentrations. Increased levels of total cholesterol (p < 0.01), high density lipoprotein cholesterol (HDL‐C) (p < 0.01), low density lipoprotein cholesterol (LDL‐C) (p < 0.01), adiponectin (p < 0.01) and ghrelin (p < 0.05) were observed at T2 in comparison with T1. No statistically significant changes were observed in serum glucose, insulin, homoeostasis model assessment for insulin sensitivity (HOMA), triglycerides and BChE. When all data of T1 and T2 were pooled, serum adiponectin showed positive correlation with progesterone (r = 0.353; p = 0.022) and HDL‐C (r = 0.307; p = 0.048), and negative with insulin (r = ?0.429; p = 0.005), HOMA (r = ?0.446; p = 0.003) and BChE (r = ?0.522; p < 0.001). Ghrelin showed negative correlation with estradiol (r = ?0.701; p = 0.004). BChE was negatively correlated with estradiol (r = ?0.441; p = 0.018) and glucose (r = ?0.343; p = 0.028), and positively with insulin (r = 0.460; p = 0.003) and HOMA (r = 0.505; p < 0.001). In conclusion, changes in metabolic biomarkers occur in bitches after LH peak, characterized by increased lipids (total cholesterol, HDL cholesterol and LDL cholesterol) without changes in BChE activity, and increased adiponectin and ghrelin concentrations, without significant changes in glucose and insulin.  相似文献   

Oestrous Synchronization,Ovarian Superovulation and Intraspecific Transfers From a Closed Breeding Colony of Inbred SLA Miniature Pigs     

JJ Arlaud  L Baker  RL Williams  AJ French 《Reproduction in domestic animals》2010,45(6):951-958

The inbred SLA miniature pig is a unique animal model developed for organ transplantation studies and pre‐clinical experimental purposes. Reported oestrous synchronization and superovulation treatments were examined in two SLA haplotypes (AA and DD) to allow collection of embryos for both practical embryo transfer and experimental technologies from a closed breeding colony. Pre‐puberal miniature pigs were poor responders to oestrous synchronization treatments, while post‐puberal sows were equivalent to commercial sows. Following superovulation, the ovulation number (corpora .hemorrhagica) was higher (p < 0.05) in the cycling sows when compared with non‐cycling sows. Ovulations were equivalent to commercial pre‐puberal gilts and non‐cycling sows (p > 0.05). No difference in ovulation number between haplotypes was observed, which differs from the previous report (DD>AA). Collection of zygotes for pronuclear injection was the highest in the non‐cycling post‐puberal miniature pig group (p < 0.05), although significantly lower when compared with the commercial pig treatment groups (p < 0.05). The incidence of cystic endometrial hyperplasia in our colony was equivalent to rates observed in commercial pigs. Pronuclear visualization following centrifugation was the highest in the non‐cycling miniature sow group and approximates to about 25% of ovulations and about half the rate observed in the commercial pigs (50%). Miniature pig embryos transferred between SLA haplotypes and transfer of DD embryos to commercial pigs resulted in live births at a higher efficiency than previously reported. This study demonstrates the feasibility of undertaking assisted reproductive technologies in a closed breeding colony of inbred SLA miniature pigs without compromise to the breeding programmes.  相似文献   

Studies of the coiled sprout disorder of the potato. 3. Effects of sprout length,planting depth and growth media     

A. H. Catchpole  J. R. Hillman 《Potato Research》1975,18(4):597-607

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Chlorpyrifos (Dursban 44r) toxicity in dairy bulls     

D H Lein  G A Maylin  R B Hillman  W C Rebhun  J D Henion  J G Ebel 《The Cornell veterinarian》1982,72(1):suppl 3-suppl58

The toxicity of Dursban 44 (chlorpyrifos) in dairy bulls in two large Northeastern A.I. bull centers is reported. One hundred eighty-five (185) bulls were treated at the first center and two hundred thirty-two (232) at the second. The application of this product resulted in the death of seven and one bulls, respectively. The knowledge and experience gained at the first center resulted in rapid diagnosis and specific treatment at the second and was felt to be a significant reason for the lower death rate. Typical, clinical signs of organophosphate poisoning were not exhibited in these two outbreaks. Specific diagnosis, treatment and the course of chlorpyrifos poisoning in Holstein bulls are discussed.  相似文献   

Inbreeding and Reproduction in Endangered Ungulates: Preservation of Genetic Variation through the Organization of Genetic Resource Banks   总被引：1，自引：0，他引：1  

ERS Roldan  M Gomendio  JJ Garde  G Espeso  S Ledda  F Berlinguer  A del Olmo  AJ Soler  L Arregui  C Crespo  R González 《Reproduction in domestic animals》2006,41(S2):82-92

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