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61.
Changes in synovial fluid and clinical variables after arthroscopic partial synovectomy of the middle carpal joint were studied in 12 normal horses. A 7 mm motorized synovial resector was inserted into each middle carpal joint; one middle carpal joint of each horse was randomly selected to have arthroscopic synovectomy (treated) and the opposite joint was lavaged (control). Lameness examinations and synovial fluid analyses were performed before operation and at 8, 14, 21, and 28 days after operation. Lameness variables did not differ between treated and control legs. Middle carpal and carpometacarpal joint circumference measurements were increased for 4 weeks. Synovial fluid specific gravity, pH, total protein, albumin concentration, and alpha-1-, beta- and gamma-globulin concentrations, at 8 and 14 days were significantly higher than before operation in both treated and control middle carpal joints. No significant differences were found between treated and control middle carpal joints at any time for color, clarity, pH, mucin clot formation, total protein, albumin, and globulin fractions. Arthroscopic partial synovectomy and lavage did not cause significant lameness and resulted in a synovitis indistinguishable from synovitis related to arthroscopic lavage alone.  相似文献   
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Pythium aphanidermatum antagonized several other fungi after hyphal contact on films of water agar, recorded by video microscopy. It sometimes coiled round, penetrated and caused cytoplasmic coagulation of hyphae of the mycoparasites P. oligandrum, P. acanthophoron, P. mycoparasiticum and P. periplocum , but was not affected by them. It also coiled round, penetrated and coagulated P. ultimum, P. vexans, P. catenulatum, P. dissotocum and (infrequently) P. graminicola. It caused cytoplasmic coagulation of Fusarium oxysporum and Trichoderma aureoviride after contact, but without coiling. Six isolates of P. aphanidermatum behaved similarly, with a mode of action like that of the mycoparasite P. oligandrum but less rapid and consistent than this species. The results are discussed in relation to possible ecological roles of P. aphanidermatum.  相似文献   
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Following the discovery of resistance to benzimidazole fungicides in the cereal eyespot pathogen Pseudocercosporella herpotrichoides in the UK in 1981, and an initial in vitro screen to select the fungicides with greatest activity against the pathogen, 40 field experiments were carried out between 1983 and 1986 to evaluate alternative fungicides for control of eyespot. At the majority of experimental sites, benomyl-resistant strains of the pathogen were present, and carbendazim did not control eyespot. Prochloraz was the most effective fungicide, reducing the eyespot index by 30–60%. There was no extra benefit from adding carbendazim to prochloraz. Flusilazole was almost as effective as prochloraz, but other fungicides had little or no effect. At sites with a high incidence of eyespot, prochloraz, with or without the addition of carbendazim, generally gave the largest yield increase. The mean yield increases each year were in the range 0·36–0·85 t/ha, and the greatest yield increase at any site was 2·27 t/ha. Most other fungicides increased yield, but carbendazim did not from 1984 to 1986. There were also yield increases at many sites with a low incidence of eyespot. Yield increases were associated with increases in thousand-grain weight at the majority of sites, but in only a few instances were there associated increases in specific weight. Prochloraz application at GS30-31 was cost effective at 71% of sites. At most sites, in regression of yield on eyespot, eyespot accounted for less than 25% of the variance in yield. The mean relationship between severe eyespot lesions and yield loss was such that each 1% increase in the percentage of tillers affected by severe eyespot was associated with a yield loss of 0·21 %. There was a significant positive correlation between eyespot at GS75 and GS30-31, and between yield increase from prochloraz treatment (at GS30-31) and eyespot at GS75, but not between yield increase and eyespot at GS30-31. The ADAS threshold for fungicide application of 20% tillers affected at GS30-31 was a reliable indicator of the cost-effectiveness of treatment at 60% of sites.  相似文献   
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Pseudomonas syringae pv. persicae , the pathogen causing bacterial decline of stone-fruit, was first noted almost simultaneously in France and in New Zealand, together with a closely related pathogen from myrobalan plum in England. The relative similarity of 31 strains from these three countries was examined by comparing DNA restriction endonuclease fragment patterns. Fragment patterns produced from digested DNA samples which were electrophoresed on polyacrylamide gels and visualized by silver-staining, were analysed using the software package Gelcompar. The fragment patterns produced by strains from France and England formed homogeneous but separate groups, while those from New Zealand were relatively heterogeneous. This finding suggests that the New Zealand population of P.s. persicae is older than those found in Europe. Problems in explaining the distribution of the pathogen are discussed.  相似文献   
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The purpose of this study was to compare computed tomography (CT) and radiography for diagnosing the presence and severity of middle ear disease in dogs with a history of chronic otitis externa. Thirty-one dogs undergoing a total ear canal ablation and bulla osteotomy were studied. Three normal dogs served as controls. All dogs were examined using radiography and CT. Three radiologists independently evaluated imaging studies in random order. A visual analog scale method was used for scoring certainty and severity of middle ear disease. Surgical findings were recorded intra-operatively. Bulla lining samples were submitted for histopathologic evaluation and scored by a single pathologist who also used a visual analog scale system. Findings from both imaging modalities agreed more closely with surgical findings than with histopathologic findings. With either surgical or histopathologic findings as the gold standard, CT was more sensitive than and as specific as radiographs for predicting presence and severity of middle ear disease. Observer performance with CT was more consistent than the performance with radiographs in the detection of changes that occur with middle ear disease. Both radiography and CT were more accurate for predicting the severity of the disease than its presence. Findings indicate that CT is more accurate and reliable than radiography in diagnosing middle ear disease for dogs having concurrent otitis externa, but only when severity of disease is moderate or high. With low severity of disease, diagnostic certainty for both modalities becomes more variable.  相似文献   
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The mean packed cell volume (PCV) for canine blood samples received by post was significantly higher than that for fresh samples, at each haemoglobin value over the range 9 to 19 g/dl. As a consequence the mean corpuscular volume (MCV) and the mean corpuscular haemoglobin concentration (MCHC) also differed. Apart from one Hb value there was no significant difference in the mean red blood cell (rbc) count over the Hb range 6 to 14 g/dl. It is suggested that the normal values for the PCV and MCV should be 3 to 5% and 5 to 7 f 1 higher respectively for postal samples as compared to fresh samples while the MCHC should be about 3 to 4 g/dl lower.  相似文献   
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