Isolation and Culture of Ovine and Bubaline Small and Large Pre-antral Follicles: Effect of Cyclicity and Presence of a Dominant Follicle     

S Nandi  V Girish Kumar  HS Ramesh  BM Manjunatha  PSP Gupta 《Reproduction in domestic animals》2009,44(1):74-79

Studies were conducted to examine the effects of the cyclicity and the presence of a dominant follicle (DF) in ovary on the recovery and in vitro growth of pre-antral follicles (PFs) in sheep and buffalo. Small pre-antral follicles (SPFs, 100–250 μm) and large pre-antral follicles (LPFs, 250–450 μm) were isolated from slaughterhouse ovaries in the breeding seasons by a mechanical and enzymatic method. The sheep and buffalo PFs were cultured in vitro for 6 and 15 days, respectively, and examined for their growth, survival and antrum formation rates and growth rates of oocytes in cultured pre-antral follicles. The follicles of the sheep and buffalo were recovered and cultured simultaneously within replicates. The recovery rates (number per ovary) of both SPFs and LPFs were significantly (p < 0.05) higher in cyclic ewes (SPFs: 22.0 ± 3.3 vs 12.1 ± 2.6 and LPFs: 16.0 ± 3.6 vs 9.2 ± 1.8) and buffaloes (SPFs: 9.2 ± 1.3 vs 4.1 ± 1.0 and LPFs: 10.3 ± 2.7 vs 5.4 ± 0.7) compared with those recovered from acyclic ones. Presence of a DF in ovary significantly (p < 0.05) reduced the recovery rates of LPFs in ewes (9.06 ± 2.7 vs 16.4 ± 3.8) but had no effect in buffalo. Cyclicity of animals or follicular dominance had no effects on in vitro growth, survival and antrum formation rates and growth rates of oocytes in cultured PFs of SPFs and LPFs in both sheep and buffalo. The in vitro growth, survival and antrum formation rates of LPFs and growth rates of oocytes in cultured LPFs were significantly (p < 0.05) higher than those observed in SPFs in both sheep and buffalo. The overall recovery and growth rates of the PFs were lower in buffaloes compared with ewes.  相似文献   

THE CONFERRING OF THE BACHELOR'S DEGREE UPON NON-GRADUATES     

Jennings WL 《Science (New York, N.Y.)》1914,40(1019):56-57

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Determination of deoxynivalenol and nivalenol chemotypes of Fusarium culmorum isolates from England and Wales by PCR assay     

P. Jennings †  M. E. Coates  J. A. Turner  E. A. Chandler  P. Nicholson 《Plant pathology》2004,53(2):182-190

Functioning Tri13 and Tri7 genes are required for the production of nivalenol and 4-acetyl nivalenol, respectively, in Fusarium species producing type B trichothecenes. Mutations have been identified in isolates which are able to produce deoxynivalenol (DON) but unable to convert this to nivalenol (NIV). In such isolates of Fusarium culmorum , the Tri7 gene is deleted entirely. PCR assays specific for functional and nonfunctional/deleted versions of Tri7 and Tri13 were used to determine the ability of 153 single spore isolates of F. culmorum to produce the 8-ketotrichothecenes deoxynivalenol and nivalenol. The isolates were collected from 76 different locations across England and Wales between 1994 and 2002. Four isolates were also obtained from one field in Scotland. Both DON and NIV chemotypes of F. culmorum were identified, with DON chemotypes predominating overall. In addition, all DON chemotypes were shown to produce 3-acetyl DON using primer sets developed to Tri3 . From fields where more than one F. culmorum isolate was obtained, isolates were not exclusively of a single chemotype. Differences in the distribution of DON and NIV chemotypes were identified, with a greater proportion of NIV chemotypes present in the south and west of England and Wales, whereas a greater proportion of DON chemotypes were found in the north and east of England. Seasonal differences in the ratio of DON:NIV chemotypes were indicated. However, these were related to seasonal variation in the distribution of F. culmorum .  相似文献   

Effects of roughage type on particle separation,rumination, fiber mat characteristics,in situ degradation,and ruminal fermentation parameters in beef steers     

Catherine L Lockard  Caleb G Lockard  Wyatt N Smith  Kendall J Karr  Ben P Holland  Alyssa B Word  Jamie L Foster  Jenny S Jennings 《Journal of animal science》2021,99(11)

Six ruminally cannulated steers (average BW = 791 ± 71 kg) were used in a replicated 3 × 3 Latin square experiment to determine the effects of roughage type on rumination, fiber mat characteristics, and rumen fermentation variables. Three roughages were included at 7% (DM basis) in a steam flaked corn-based diet: cotton burrs (CB), wheat silage (WS), or corn stalks (CS). Steers were fitted with a sensory collar to record rumination behaviors in 2-h intervals at the beginning of the experiment. Each 30-d period consisted of 7 d of recovery, 14 d of diet adaptation, 7 d of rumination data collection (daily and bi-hourly average rumination), 1 d of rumen fluid collection, and 1 d of rumen evacuations. In situ degradation of individual roughages was determined for 4 d after period 3 evacuations. During rumen evacuations, ruminal contents were removed; the rumen fiber mat (RF) was separated from the liquid portion with a 2-mm sieve, weighed, and a subsample was dried. Data were analyzed using the MIXED procedure of SAS with steer as the experimental unit and roughage (CB, WS, and CS) as the main effect. Dry matter intake (DMI) was not different for CB and WS (P = 0.25) and greatest for steers consuming CS diet (P ≤ 0.01). Roughage type did not influence the weight of the RF dry matter (%; DM; P = 0.92), RF weight (P = 0.69), or RF:DMI ratio (P = 0.29). Daily rumination (min/d) did not differ among roughages (P = 0.40), but min of rumination/kg of DMI was greatest for CS (18.0 min), min/kg of NDF was greatest for WS (89.8 min; P = 0.02), and min/kg of peNDF was greatest for CS (132.4 min; P ≤ 0.01). Wheat silage had the greatest percentage of soluble and degradable DM. Rumen fiber mat did not differ for roughages, although rumination min/kg of DMI and peNDF was greatest for steers consuming CS and WS. In situ degradation determined that CB-R and CS-R had the greatest percentage of ruminal undegraded DM. Based on the objective of the experiment, roughage type did not influence daily rumination or fiber mat characteristics.  相似文献   

Effects of Vegetation-Free Strip Width and Irrigation on Newly Planted Peach     

Juliana K. Buckelew  Wayne E. Mitchem  David W. Monks  Katie M. Jennings  Lucky K. Mehra 《International Journal of Fruit Science》2019,19(1):75-90

Field experiments were conducted at two locations (Clayton and Jackson Springs, NC) to determine the influence of vegetation-free strip width (VFSW) and irrigation on newly planted peach growth and yield in a low-density orchard with a volunteer weedy ground cover. The experiments included VFSW of 0, 0.6, 1.2, 2.4, 3, or 3.6 m under irrigated or nonirrigated conditions. Seasonal variation in the orchard floor vegetation was observed as different weed species reported in summer and winter. However, this difference was not apparent with respect to VFSF and irrigation. At Jackson Springs, NC, the predicted irrigated VFSW which would produce the same trunk cross-sectional area (TCSA) as the grower standard (3-m nonirrigated) was 1.5, 1.3, and 0.8 m for one-, two-, and three-year-old trees, respectively. The predicted irrigated VFSW which would produce the same yield as the grower standard was 1.16 m. At Clayton, TCSA and fruit yield were not different by irrigation, but did increase linearly with VFSW. At both locations, leaf nitrogen (N) concentration was lower in irrigated trees than nonirrigated trees. Leaf N, leaf area, and SPAD were positively related to VFSW at Jackson Springs. In contrast, leaf N concentration was not different by VFSW at Clayton. However, leaf area and SPAD were positively related to VFSW at Clayton. These results suggest that a 1.5 m VFSW combined with proper irrigation and fertilization will produce tree growth and yield in newly planted orchard with volunteer weedy vegetation similar to the current grower standard in the southeastern USA.  相似文献   

Percutaneous balloon valvuloplasty for treatment of pulmonic stenosis in a dog   总被引：2，自引：0，他引：2  

J M Bright  J Jennings  R Toal  M E Hood 《Journal of the American Veterinary Medical Association》1987,191(8):995-996

A 2.5-year-old dog with congenital pulmonary valve dysplasia was treated by percutaneous balloon dilation of the stenotic orifice. Clinical and hemodynamic improvement was apparent immediately and for 9 months after the valvuloplasty procedure.  相似文献   

The efficac of nitroxynil in the treatment of naturally-occurring ovine fascioliasis     

J F Reid  J Armour  F W Jennings  G M Urquhart 《The Veterinary record》1970,86(2):41-42

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Inhibition of Ostertagia ostertagi at the early fourth larval stage. II. The influence of environment on host or parasite     

J Armour  F W Jennings  G M Urquhart 《Research in veterinary science》1969,10(3):238-244

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Compensatory growth after under-nutrition in market turkeys     

J N Auckland  T R Morris  R C Jennings 《British poultry science》1969,10(4):293-302

Five levels of protein were fed from o to 6 weeks of age, resulting in groups of poults whose body weights at 6 weeks were depressed by 8, 25 and 50 per cent below maximum. After 6 weeks, all groups were fed adequate amounts of protein and the undernourished poults showed substantial compensatory growth. The treatment which resulted in a mean body weight 25 per cent below controls at 6 weeks gave only a 3 per cent reduction in live‐weight at 14 weeks of age.

Undernourished poults ate more food at any given live‐weight between 6 and 14 weeks of age than did fully fed controls when they were at the same live‐weight. Food conversion was better in undernourished than in fully fed poults in the 6 to 14 week period even after a correction had been made for differences in the food required to maintain different body weights.

Taking the whole period from o to 14 weeks, there was a small improvement in the efficiency of food conversion and a large increase in the efficiency of protein conversion to body weight in favour of the poults which had been undernourished to 6 weeks of age.  相似文献   

Production of pneumonia in gnotobiotic pigs with pure cultures of mycoplasma hyopneumoniae   总被引：3，自引：0，他引：3  

R T Hodges  A O Betts  A R Jennings 《The Veterinary record》1969,84(11):268-273

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