First Evidence that Ecklonia cava-Derived Dieckol Attenuates MCF-7 Human Breast Carcinoma Cell Migration     

Eun-Kyung Kim  Yujiao Tang  Yon-Suk Kim  Jin-Woo Hwang  Eun-Ju Choi  Ji-Hyeok Lee  Seung-Hong Lee  You-Jin Jeon  Pyo-Jam Park 《Marine drugs》2015,13(4):1785-1797

We investigated the effect of Ecklonia cava (E. cava)-derived dieckol on movement behavior and the expression of migration-related genes in MCF-7 human breast cancer cell. Phlorotannins (e.g., dieckol, 6,6′-biecko, and 2,7″-phloroglucinol-6,6′-bieckol) were purified from E. cava by using centrifugal partition chromatography. Among the phlorotannins, we found that dieckol inhibited breast cancer cell the most and was selected for further study. Radius™-well was used to assess cell migration, and dieckol (1–100 µM) was found to suppress breast cancer cell movement. Metastasis-related gene expressions were evaluated by RT-PCR and Western blot analysis. In addition, dieckol inhibited the expression of migration-related genes such as matrix metalloproteinase (MMP)-9 and vascular endothelial growth factor (VEGF). On the other hand, it stimulated the expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2. These results suggest that dieckol exerts anti-breast cancer activity via the regulation of the expressions of metastasis-related genes, and this is the first report on the anti-breast cancer effect of dieckol.  相似文献   

Porcine Granulocyte-Macrophage Colony-Stimulating Factor Improves the in vitro Development of Cloned Porcine Embryos     

Kwak SS  Cheong SA  Jeon Y  Hyun SH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(9):1095-1102

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Effects of repetitive ionomycin treatment on in vitro development of bovine somatic cell nuclear transfer embryos     

Kim HS  Lee JY  Jeong EJ  Yang CJ  Hyun SH  Shin T  Hwang WS 《The Journal of reproduction and development》2012,58(1):132-139

To artificially activate embryos in somatic cell nuclear transfer (SCNT), chemical treatment with ionomycin has been used to induce transient levels of Ca(2+) and initiate reprogramming of embryos. Ca(2+) oscillation occurs naturally several times after fertilization (several times with 15- to 30-min intervals). This indicates how essential additional Ca(2+) influx is for successful reprogramming of embryos. Hence, in this report, the experimental design was aimed at improving the developmental efficiency of cloned embryos by repetitive Ca(2+) transients rather than the commonly used ionomycin treatment (4 min). To determine optimal Ca(2+) inflow conditions, we performed three different repetitive ionomycin (10 μM) treatments in reconstructed embryos: Group 1 (4-min ionomycin treatment, once), Group 2 (30-sec treatment, 4 times, 15-min intervals) and Group 3 (1-min treatment, 4 times, 15-min intervals). Pronuclear formation rates were checked to assess the effects of repetitive ionomycin treatment on reprogramming of cloned embryos. Cleavage rates were investigated on day 2, and the formation rates of blastocysts (BLs) were examined on day 7 to demonstrate the positive effect of repeated ionomycin treatment. In Group 3, a significant increase in BL formation was observed [47/200 (23.50%), 44/197 (22.33%) and 69/195 (35.38%) in Groups 1, 2 and 3, respectively]. Culturing embryos with different ionomycin treatments caused no significant difference among the groups in terms of the total cell number of BLs (164.3, 158.5 and 145.1, respectively). Additionally, expression of the anti-apoptotic Bcl-2 gene and MnSOD increased significantly in Group 3, whereas the expression of the pro-apoptotic Bax decreased statistically. In conclusion, the present study demonstrated that repeated ionomycin treatment is an improved activation method that can increase the developmental competence of SCNT embryos by decreasing the incidence of apoptosis.  相似文献   

Neurogenic and cardiomyogenic differentiation of mesenchymal stem cells isolated from minipig bone marrow     

Kumar BM  Maeng GH  Lee YM  Kim TH  Lee JH  Jeon BG  Ock SA  Yoo JG  Rho GJ 《Research in veterinary science》2012,93(2):749-757

The present study investigated the potential of minipig bone marrow-mesenchymal stem cells (BM-MSCs) to differentiate in vitro into neuron- and cardiomyocyte-like cells. Isolated BM-MSCs exhibited a fibroblast-like morphology, expressed CD29, CD44 and CD90, and differentiated into osteocytes, adipocytes and chondrocytes. Upon induction in two different neuronal specific media, most of BM-MSCs acquired the distinctive morphological features and positively stained for nestin, neurofilament-M (NF-M), neuronal nuclei (NeuN), β-tubulin, galactocerebroside (Gal-C) and glial fibrillary acidic protein (GFAP). Expression of nestin, GFAP and NF-M was further demonstrated by RT-PCR and RT-qPCR. Following cardiomyogenic induction, MSCs exhibited a stick-like morphology with extended cytoplasmic processes, and formed cluster-like structures. The expression of cardiac specific markers α-smooth muscle actin, cardiac troponin T, desmin and α-cardiac actin was positive for immunofluorescence staining, and further confirmed by RT-PCR and RT-qPCR. In conclusion, our results showed the in vitro differentiation ability of porcine BM-MSCs into neuron-like and cardiomyocyte-like cells.  相似文献   

Clinical Relationship between Cholestatic Disease and Pituitary‐Dependent Hyperadrenocorticism in Dogs: A Retrospective Case Series          下载免费PDF全文

K.‐h. Kim  S.‐m. Han  K.‐o. Jeon  H.‐t. Kim  Q. Li  M.‐o. Ryu  W.‐j. Song  S.‐c. Park  H.‐y. Youn 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2017,31(2):335-342

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兔球虫与大肠杆菌病混合感染的诊治     

王俊江  单红艳  王文木 《中国兽药杂志》2007,41(8):48-48,53

阐述了兔球虫与大肠杆菌病混合感染的临床症状、实验室检验及防治方法,力求为科学防控两病提供有益的参考。  相似文献   

Long-term evaluation of the effects of vitreous degeneration on cataracts and retinal detachment in dogs     

Byung-Ju Jeon  Suk-Jun Lee  Kyu-Lee Kim  Eun-A Huh  Joon-Young Kim 《Veterinary ophthalmology》2023,26(4):324-330

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Performance of the SD Bioline TB Ag MPT64 Rapid test for quick confirmation of Mycobacterium bovis isolates from animals     

Hyeon-Seop Byeon  Mi Jung Ji  Shin-Seok Kang  Sang Woo Kim  Seung-Cheol Kim  Song-Yong Park  Geehyuk Kim  Jiro Kim  Jang-Eun Cho  Bok Kyung Ku  Jae-Myung Kim  Bo-Young Jeon 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):31-35

Mycobacterium (M.) bovis, a bacterium in the M. tuberculosis complex, is a causative agent of bovine tuberculosis, a contagious disease of animals. Mycobacterial culture is the gold standard for diagnosing bovine tuberculosis, but this technique is laborious and time-consuming. In the present study, performance of the SD Bioline TB Ag MPT4 Rapid test, an immunochromatographic assay, was evaluated using reference bacterial strains and M. bovis field isolates collected from animals. The SD MPT64 Rapid test produced positive results for 95.5% (63/66) of the M. bovis isolates from cattle and 97.9% (46/47) of the isolates from deer. Additionally, the test had a sensitivity of 96.5% (95% CI, 91.2-99.0), specificity of 100% (95% CI, 96.7-100.0), positive predictive value of 100% (95% CI, 96.7-100.0), and negative predictive value of 92.9% (95% CI, 82.7-98.0) for M. bovis isolates. In conclusion, the SD MPT64 Rapid test is simple to use and may be useful for quickly confirming the presence of M. bovis in animals.  相似文献   

Canine model of ischemic stroke with permanent middle cerebral artery occlusion: clinical features,magnetic resonance imaging,histopathology, and immunohistochemistry     

Joon-Hyeok Jeon  Hae-Won Jung  Hyo-Mi Jang  Jong-Hyun Moon  Ki-Tae Park  Hee-Chun Lee  Ha-Young Lim  Jung-Hyang Sur  Byeong-Teck Kang  Jeongim Ha  Dong-In Jung 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(1):75-85

The purpose of this study was to identify time-related changes in clinical, MRI, histopathologic, and immunohistochemical findings associated with ischemic stroke in dogs. Additionally, the association of cerebrospinal fluid (CSF) and tissue levels of interleukin (IL)-6 with clinical prognosis was assessed. Ischemic stroke was induced by permanent middle cerebral artery occlusion (MCAO) in nine healthy experimental dogs. The dogs were divided into three groups according to survival time and duration of the experimental period: group A (survived only 1 day), group B (1-week experimental period), and group C (2-week experimental period). Neurologic status was evaluated daily. Magnetic resonance imaging (MRI) was performed according to a predetermined schedule. Concentration of IL-6 in CSF was measured serially after ischemic stroke. Postmortem examination was performed for all experimental dogs. During histopathological examination, variable degrees of cavitation and necrosis due to neuronal cytopathic effects, such as pyknotic nuclei and cytoplasmic shrinkage, were observed on the affected side of the cerebral cortex in all dogs. Immunohistochemistry specific for IL-6 showed increased expression in the ischemic lesions. CSF IL-6 concentrations and ischemic lesion volumes 1 day after ischemic stroke were significantly higher in group A compared to groups B and C.  相似文献   

Helicobacter apodemus sp. nov., a new Helicobacter species identified from the gastrointestinal tract of striped field mice in Korea     

Woo Jin Jeon  Hee-Jin Dong  Jae Hoon Shin  Il Yong Kim  Hungwui Ho  Seung Hyun Oh  Young Min Yoon  Yang-Kyu Choi  Jun Gyo Suh  Ki-Hoan Nam  Hyoung-Chin Kim  Seongbeom Cho  Je Kyung Seong 《Journal of veterinary science (Suw?n-si, Korea)》2015,16(4):475-481

A novel Helicobacter species was identified from the gastrointestinal tract of the Korean striped field mouse (Apodemus agrarius). Biochemical testing, ultrastructure characterization, and 16S rRNA gene sequence analysis suggested that this bacterium represents a distinct taxon. The bacterium was positive for urease activity, susceptible to cephalothin and nalidixic acid, and weakly positive for oxidase and catalase activity. Electron microscopy revealed that the bacterium has spirally curved rod morphology with singular bipolar nonsheathed flagella. Genotypically, the isolated bacterial strains (YMRC 000215, YMRC 000216, and YMRC 000419) were most closely related to a reference strain of Helicobacter mesocricetorum (97.25%, 97.32%, and 97.03% 16S rRNA sequence similarities, respectively). The 16S rRNA sequences of these strains were deposited into GenBank under accession numbers {"type":"entrez-nucleotide","attrs":{"text":"AF284754","term_id":"18389594","term_text":"AF284754"}}AF284754, {"type":"entrez-nucleotide","attrs":{"text":"AY009129","term_id":"18409527","term_text":"AY009129"}}AY009129, and {"type":"entrez-nucleotide","attrs":{"text":"AY009130","term_id":"18409528","term_text":"AY009130"}}AY009130, respectively. We propose the name Helicobacter apodemus for this novel species.  相似文献