Dilation of the olfactory bulb cavity concurrent with hydrocephalus in four small breed dogs     

Jung-Hyun Kim  Hyo-Won Jeon  Eung-Je Woo  Hee-Myung Park 《Journal of veterinary science (Suw?n-si, Korea)》2009,10(2):173-175

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Identification of SNPs in MYOD gene family and their associations with carcass traits in cattle     

M.S.A. Bhuiyan  N.K. Kim  Y.M. Cho  D. Yoon  K.S. Kim  J.T. Jeon  J.H. Lee   《Livestock Science》2009,126(1-3):292-297

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Cellular Composition and Viability of Cloned Bovine Embryos Using Exogene-Transfected Somatic Cells     

S-L Lee  BM Kumar  J-G Kim  S-A Ock  B-G Jeon  S Balasubramanian  S-Y Choe  G-J Rho 《Reproduction in domestic animals》2007,42(1):44-52

The present study compared the efficiency of transgenic (TG) cloned embryo production by somatic cell nuclear transfer (SCNT) with fetal-derived fibroblast cells (FFCs) which were transfected with pEGFP-N1 to in vitro-fertilized (IVF), parthenogenetic and SCNT counterparts by evaluating the rates of cleavage and blastocyst formation, apoptosis rate at different developmental stages, cell number, ploidy and gene expression in blastocysts. In SCNT and TG embryos, the rates of cleavage and blastocyst formation were significantly lower (p < 0.05) than those of IVF controls, but it did not differ between SCNT and TG embryos. In IVF control, 86.7% embryos displayed diploid chromosomal complements and the rates were significantly (p < 0.05) higher than those of SCNT and TG embryos. Most TG embryos (79%) with FFCs expressed the gene by both PCR and under fluorescence microscopy. The expression of apoptosis by TUNEL was first detected at six to eight cell stages in all embryos of IVF, SCNT and TG groups, but the expression rate at each developmental stages was significantly higher (p < 0.05) in SCNT and TG embryos than in IVF counterparts. The expression rate in inner cell mass (ICM) of TG embryos was significantly higher (p < 0.05) than in SCNT and IVF embryos. These results indicate that the high occurrence of apoptosis observed in SCNT and TG embryos compared with IVF counterparts might influence the developmental competence. Moreover, the SCNT embryos derived using non-transfected donor cells exhibited a lower apoptosis expression in ICM cells than in TG embryos derived using pEGP-N1-transfected donor cells suggesting a possible role of negative gene effect in TG embryos.  相似文献   

cDNA microarray gene expression analysis and toxicological phenotype for anticancer drug   总被引：1，自引：0，他引：1  

Lee GJ  Lee WS  Jeon KS  Um CH  Kim YS  Kim SJ  Lee CH  Yoon HK  Hwang SY  Park JS  Hwang JW  Kang KS  Lee YS  Kim MS  Chon KJ  Yeo CD  Kang JS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1339-1345

Toxicogenomics, the subdiscipline that merges genomics with toxicology, hold the promise to contributing toward the goal of elucidating mechanism by studying genomic profiling related with various drugs. The application of gene expression profiling technology to examine multiple genes and signaling pathways promises a significant advance in understanding the toxic mechanisms of various drugs and prediction of new drug candidate. Toxicogenomics is emerging field combining genomics and bioinformatics to identify and characterize mechanisms of toxicity of drug and various compounds. The principal hypothesis underlying on this field is that chemical-specific pattern of altered gene expression is related with each chemicals properties, especially toxicological property, and it will be revealed using high-density microarray analysis of sample from exposed organisms. So, in this study we compare the gene expression pattern of two anticancer drugs paclitaxel and orally absorbable paclitaxel, using the cDNA microarray. And from the result of this study, it is possible to provide the new possibility for genome-wide insight into mechanism of their anticancer activity and toxicological phenotype.  相似文献   

Evaluation of Dietary Fishmeal Analogue with Addition of Shrimp Soluble Extract on Growth and Nonspecific Immune Response of Rainbow Trout,Oncorhynchus mykiss          下载免费PDF全文

Hayun Jo  Seunghan Lee  Hyeonho Yun  Jeongwhui Hong  Mohammad Moniruzzaman  Sungchul C. Bai  Gunhyun Park  Seokwoo Chee  Tae‐Eun Jeon 《Journal of the World Aquaculture Society》2017,48(4):583-591

A 12‐wk feeding trial was conducted to evaluate the effects of the dietary fishmeal analogue (FMA) adding 2% shrimp soluble extract (SSE) on growing rainbow trout, Oncorhynchus mykiss. Fish averaging 146 ± 3.8 g (mean ± SD) were randomly distributed into 500‐L aquaria as groups of 20 fish per tank. Fish were fed the experimental diets in triplicate groups to apparent satiation twice a day on a dry matter basis. Each group was fed one of the four experimental diets: control (FM100% + FMA0%), FMA₀ (FM100% + FMA0% + SSE2%), FMA₁₂ (FM88% + FMA12% + SSE2%), and FMA₂₄ (FM76% + FMA24% + SSE2%). After the feeding trial, weight gain and specific growth rate of fish fed the FMA₀ and FMA₁₂ diets were significantly higher than those of fish fed the FMA₂₄ diet (P < 0.05). Feed efficiency and protein efficiency ratio values of fish fed the control, FMA₀, and FMA₁₂ diets were significantly higher than those of fish fed the FMA₂₄ diet (P < 0.05). The values of superoxide dismutase activity showed significantly higher amounts for the FMA₀, FMA₁₂, and FMA₂₄ groups than the control group (P < 0.05). Lysozyme activity in the FMA₁₂ group was significantly higher than in the control group (P < 0.05). Therefore, according to our results, FMA with additive SSE can be used up to 12% as a substitute of fishmeal for growing rainbow trout.  相似文献   

Congenital lymphangiomatosis and an enteric duplication cyst in a young dog     

H. Oui  C. Lamm  S. Stiver  B. Williams  S. Y. Kwon  Y. Bae  S. Jeon  S. Park  J. J. Min  J. Choi 《The Journal of small animal practice》2014,55(7):379-382

A two‐year‐old female poodle with abdominal distention was diagnosed with concurrent enteric duplication cyst and lymphangiomatosis. Both lesions were shown as cystic structures, but some characteristic features of enteric duplication cyst were identified including a thick cyst wall and shared blood supply with the duodenum. Although it was challenging to discriminate between the types of cyst based on diagnostic imaging, this report describes the characteristics of each type of lesion using several different imaging modalities.  相似文献   

Reactive oxygen scavenging effect of enzymatic extracts from Sargassum thunbergii   总被引：1，自引：0，他引：1  

Park PJ  Heo SJ  Park EJ  Kim SK  Byun HG  Jeon BT  Jeon YJ 《Journal of agricultural and food chemistry》2005,53(17):6666-6672

The free radical scavenging activity of water soluble natural antioxidants from Sargassum thunbergii, which is a brown marine alga, was evaluated by examining the radical scavenging activities of the extracts of hydrolyzates from S. thunbergii on hydroxyl, 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radicals. A spin-trapping electron spin resonance (ESR) spectrometer was employed, and the results were compared for their ESR signal intensity. S. thunbergii was enzymatically hydrolyzed to prepare water soluble extracts by five carbohydrases (AMG, Celluclast, Termamyl, Ultraflo, and Viscozyme) and proteases (Alcalase, Flavorzyme, Kojizyme, Neutrase, and Protamex). The scavenging activity of the radicals increased with increased concentrations of the extracts. The scavenging results were higher for hydroxyl and alkyl radicals and lower for DPPH radical as compared with vitamin C as a reference. The hydrogen peroxide scavenging activity of the extracts was also investigated; the Alcalase extract showed the highest scavenging activity among the extracts prepared with the five proteases and five carbohydrates. In addition, the DNA damage was determined by using the comet assay with alkaline electrophoresis and was quantified by measuring the tail length. The preventive effect of Alcalase extract from S. thunbergii against DNA damage increased with increments of concentration of the enzymatic extracts.  相似文献   

Sr Isotopic Signature in Plant-Derived Ca in Rain     

Takanori Nakano  Seo-Ryong Jeon  Junko Shindo  Tamon Fumoto  Naoki Okada  Jun Shimada 《Water, air, and soil pollution》2001,130(1-4):769-774

The ⁸⁷Sr/⁸⁶Sr ratios in rain at three sites in Japan were about 0.706 to 0.712, whereas those at one site in central Korea were higher (0.711 to 0.716), reflecting the difference in geology between the two countries. Because the ⁸⁷Sr/⁸⁶Sr ratios of exchangeable sites in soil minerals are indistinguishable from those of associated plants, aerosol Sr originating from both can be grouped together as plant-derived Sr. Spatial and temporal variations in ⁸⁷Sr/⁸⁶Sr and Sr/Ca ratios in the rain suggest the presence of plant-derived Sr and Ca in addition to Sr and Ca derived from sea-salt, acid-soluble aeolian carbonate from China, and cement-derived carbonate. However, systematic data on the ⁸⁷Sr/⁸⁶Sr ratios and elemental concentrations of soils, plants, fly-ash, and road dust are required to put reliable constraints on the provenance of atmospheric Ca.  相似文献   

Molecular cloning of vitellogenin cDNA in rockfish (Sebastes schlegeli) and effects of estrogenic substances on its gene expression     

Jee-Hyun Jung  Yong-Chang Sohn  Jae-Yong Lee  Dae-Jung Kim  Ji-Seon Lee  Joong-Kyon Jeon  Chang-Hee Han 《Fish physiology and biochemistry》2003,28(1-4):419-420

In this study, we have cloned a partial cDNA of 620 bases encoding a vitellogenin gene of rockfish (rfVTG), Sebastes schlegeli. The expression levels of rfVTG mRNA increased 48 h after the 4-nonylphenol (NP) treatment (10 mg/kg BW) in both sexes. On the other hand, the mRNA levels of rfVTG increased 48 h in males and 12 h in females after injection when higher dose (25 mg/kg BW) of 4-NP was applied. It suggested that 4-NP induced VTG gene expression in both male and female immature rockfish. Therefore, the cloned rfVTG cDNA in this study can be used as a tool for monitoring endocrine disruptors in rockfish.  相似文献   

<Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated transformation of <Emphasis Type="Italic">Brachypodium distachyon</Emphasis> inbred line Bd21 with two binary vectors containing hygromycin resistance and GUS reporter genes     

Man Bo Lee  Woong Bae Jeon  Dae Yeon Kim  Odgerel Bold  Min Jeong Hong  Yong Jin Lee  Jae Han Park  Yong Weon Seo 《Journal of Crop Science and Biotechnology》2011,14(4):233-238

Brachypodium distachyon (Brachypodium) is a novel model plant for structural and functional genomic studies of temperate grasses. Brachypodium as a model plant has many favorable features, such as small size, small genome, short life cycle, self-fertility, and simple growth requirements. The genome sequence of the standard line Bd21 has been released and genomic resources have been developed.It is imperative to develop a method for efficient Agrobacterium-mediated Brachypodium transformation. Yellowish and compact embryogenic calli derived from immature embryos of the Bd21 were transformed with the Agrobacterium strain AGL1. Seven- and nine-week-old calli were used for transformation with Agrobacterium carrying either pCAMBIA 1301 and pCAUGH. Transformation efficiencies were assessed through histochemical GUS assay. The efficiency of transformation with pCAMBIA 1301 (based on the number of callus lines producing GUS-detected plantlets and the number of calli used for transformation) reached 20.1% (7-week-old calli) and 1.7% (9-week-old calli), and with pCAUGH (based on the number of GUS-detected plantlets and the number of regenerants) 90 and 87% for 7- and 9-week-old calli, respectively. High selection pressure was obtained by using pCAUGH, which is preferred for saving labor and time consumption during the callus selection.  相似文献