Diagnostic utility of egg yolk for the detection of avian metapneumovirus antibodies in laying hens     

Choi KS  Lee EK  Jeon WJ  Park MJ  Yoo YN  Kwon JH 《Avian diseases》2010,54(4):1230-1236

Surveillance and diagnosis of avian metapneumovirus (AMPV) infection typically involve measurement of serum antibodies. In the current study, eggs instead of serum samples were used for the detection of AMPV antibodies in egg-laying chicken hens by enzyme-linked immunosorbent assay (ELISA). AMPV-free commercial layer hens were experimentally challenged with AMPV strain SC1509 through intravenous or oculonasal administration. Antibody levels were determined by ELISA. AMPV antibodies were detected in egg yolks from challenged hens by 7 days postinoculation (dpi), with the peak titer at 16 dpi. Antibody levels in eggs laid at 28 dpi correlated well (r = 0.93) with sera taken 28 dpi from the same hens. In a field trial of the yolk ELISA, six broiler breeder farms were surveyed, and all tested positive for AMPV antibodies in hen eggs, although positivity varied from farm to farm. Abnormal discolored eggs collected from outbreak farms had significantly higher titers of AMPV yolk antibodies than normal eggs from the same farm, unlike clinically healthy farms, where normal and abnormal eggs had similar antibody titers. These results indicate that diagnosis of AMPV infection by yolk ELISA to detect anti-AMPV antibodies may be a suitable alternative to serologic testing.  相似文献   

Ethanol extract of Angelica gigas inhibits croton oil-induced inflammation by suppressing the cyclooxygenase - prostaglandin pathway     

Sunhee Shin  Seong Soo Joo  Dongsun Park  Jeong Hee Jeon  Tae Kyun Kim  Jeong Seon Kim  Sung Kyeong Park  Bang Yeon Hwang  Yun-Bae Kim 《Journal of veterinary science (Suw?n-si, Korea)》2010,11(1):43-50

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Acaricidal constituents isolated from Sinapis alba L. seeds and structure-activity relationships     

Lim JH  Kim HW  Jeon JH  Lee HS 《Journal of agricultural and food chemistry》2008,56(21):9962-9966

Allyl isothiocyanate (AITC) and phenethyl isothiocyanate (PEITC) were isolated from Sinapis alba L. seeds and their effects against Dermatophagoides farinae and D. pteronyssinus were evaluated using the impregnated fabric disk method. The LD 50 values of their compounds and derivatives were then compared with those of a commercial acaricide, benzyl benzoate. On the basis of the LD 50 values against D. farinae, PEITC (0.21 microg/cm(2)) was the most toxic, followed by benzyl isothiocyanate (0.55 microg/cm(2)), phenyl isothiocyanate (1.09 microg/cm(2)), butyl isothiocyanate (1.24 microg/cm(2)), and AITC (1.36 microg/cm(2)); acetyl isothiocyanate (195.01 microg/cm(2)) was the least toxic. In addition, the acaricidal effects of AITC and PEITC against D. farinae were 7.4- and 47.8-fold greater than those of benzyl benzoate, respectively. Against D. pteronyssinus, PEITC was the most toxic (0.19 microg/cm(2)), followed by benzyl isothiocyanate (0.77 microg/cm(2)), phenyl isothiocyanate (1.37 microg/cm(2)), butyl isothiocyanate (1.50 microg/cm(2)), and AITC (2.88 microg/cm(2)); acetyl isothiocyanate (168.82 microg/cm(2)) was the least toxic. AITC and PEITC were 3.3- and 50.4-fold more active than benzyl benzoate against D. pteronyssinus, respectively. Taken together, these findings indicate that AITC, PEITC, and partial derivatives may be useful as preventive agents against dust mites. In addition, these results indicate that structure-activity is related to the aromatic structure, the number of carbon atoms, and the compounds hydrophobicity.  相似文献   

Effects of rindite on breaking dormancy of potato microtubers     

Hyun Soon Kim  Jae Heung Jeon  Kyung Hwa Choi  Young Hee Joung  Hyouk Joung 《American Journal of Potato Research》1999,76(1):5-8

Potato microtubers were treated with rindite to investigate the effect on dormancy breaking. Postharvest application of rindite by fumigation with 2 ml rindite for 48 hr or 4ml for 24 hr significantly reduced the dormancy period of potato microtubers using a 32 x 15 x 17 cm tightly sealed plastic box. Approximately 2 weeks after the treatments microtubers of all cultivars, Atlantic, Superior, Lemhi Russet, Red Dale and Kennebec started to sprout. The efficiency of the treatments were the greatest for the cv. Lemhi Russet, intermediate for cv. Superior and least for cv. Red Dale. In all cultivars of potato microtubers, more decay was observed the earlier rindite treatment occurred after harvesting, therefore potato microtubers should be treated with rindite at least 2 weeks after harvest when the skin of microtubers is mature. The data indicates that the dormancy of potato microtubers with well-matured skin can be effectively broken with an optimum treatment of rindite.  相似文献   

Historical deposition of trace metals in a marine sapropel from Mangrove Lake,Bermuda with emphasis on mercury,lead, and their isotopic composition     

Jeon  Byunggwon  Scircle  Austin  Cizdziel  James V.  Chen  Jingjing  Black  Oscar  Wallace  Davin J.  Zhou  Ying  Lepak  Ryan F.  Hurley  James P. 《Journal of Soils and Sediments》2020,20(4):2266-2276

Journal of Soils and Sediments - Sapropel is an organic-rich sediment formed under conditions that can result in sequestration of trace metals. Here, we determined the concentration of total...  相似文献   

Ishophloroglucin A,Isolated from Ishige okamurae,Alleviates Dexamethasone-Induced Muscle Atrophy through Muscle Protein Metabolism In Vivo     

Hye-Won Yang  Seyeon Oh  Dong-Min Chung  Minyoung Seo  Shin Jae Park  You-Jin Jeon  Kyunghee Byun  BoMi Ryu 《Marine drugs》2022,20(5)

The in vitro capacity of Ishige okamurae extract (IO) to improve impaired muscle function has been previously examined. However, the mechanism underlying IO-mediated muscle protein metabolism and the role of its component, Ishophloroglucin A (IPA), in mice with dexamethasone (Dexa)-induced muscle atrophy remains unknown. In the present study, we evaluated the effect of IO and IPA supplementation on Dexa-induced muscle atrophy by assessing muscle protein metabolism in gastrocnemius and soleus muscles of mice. IO and IPA supplementation improved the Dexa-induced decrease in muscle weight and width, leading to enhanced grip strength. In addition, IO and IPA supplementation regulated impaired protein synthesis (PI3K and Akt) or degradation (muscle-specific ubiquitin ligase muscle RING finger and atrogin-1) by modulating mRNA levels in gastrocnemius and soleus muscles. Additionally, IO and IPA upregulated mRNA levels associated with muscle growth activation (transient receptor potential vanilloid type 4 and adenosine A1 receptor) or inhibition (myostatin and sirtuin 1) in gastrocnemius and soleus muscle tissues of Dexa-induced mice. Collectively, these results suggest that IO and IO-derived IPA can regulate muscle growth through muscle protein metabolism in Dexa-induced muscle atrophy.  相似文献   

New 2-phenyl-4,5,6,7-tetrahydro-2H-indazole derivatives as paddy field herbicides     

Hwang IT  Kim HR  Jeon DJ  Hong KS  Song JH  Chung CK  Cho KY 《Pest management science》2005,61(5):483-490

A series of 3-chloro-2-(4-chloro-2-fluorophenyl)-4,5,6,7-tetrahydro-2H-indazole derivatives containing various substituted isoxazolinylmethoxy groups at the 5-position of the benzene ring were synthesized and their herbicidal activities assessed under greenhouse and flooded paddy conditions. Among them, compounds having a phenyl or cyano substituent at the 3-position of the 5-methyl-isoxazolin-5-yl structure demonstrated good rice selectivity and potent herbicidal activity against annual weeds at 16-63 g AI ha(-1) under greenhouse conditions. Field trials indicated that these two compounds controlled a wide range of annual weeds rapidly with a good tolerance on transplanted rice seedlings by pre-emergence application. They showed a low mammalian and environmental toxicity in various toxicological tests.  相似文献   

The intelligent data management system for toxicogenomics     

Lee WS  Lee GJ  Lee GJ  Yeo CD  Kang JS  Kim YH  Kim SJ  Kim JS  Hwang SY  Park JS  Hwang JW  Kang KS  Lee YS  Jeon KS  Um CH  Hong SI  Kim YS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1335-1338

Toxicogenomics is now emerging as one of the most important genomic application because the toxicity test based on gene expression profiles is expected to be more precise and efficient than current histopathological approaches in a pre-clinical phase. One of the challenging issues in toxicogenomics is the construction of intelligent database management system which can deal with heterogeneous and complex data from many different experimental and information sources. TEST(Toxicogenomics for Efficient Safety Test) database is especially focused on the connectivity of heterogeneous data and the intelligent query system which enable users to obtain relevant useful information from the complex data sets. The database deals with four kinds of information; compound, histopathology, gene expression, and annotation information. Currently, TEST database maintains toxicogenomics information for 16 compounds, 45 microarrays, 190 animal experiments, and customized 4.8 K rat clone set. Our presented system is expected to be a good information source for studying of toxicology mechanism in the genome-wide level and can also be applied to the designing toxicity test chip.  相似文献   

Synthesis and fungicidal activity of ethaboxam against Oomycetes     

Kim DS  Chun SJ  Jeon JJ  Lee SW  Joe GH 《Pest management science》2004,60(10):1007-1012

This study describes the chemical synthesis and intrinsic fungicidal activity of ethaboxam [(RS)-N-(alpha-cyano-2-thenyl)-4-ethyl-2-(ethylamino)-1,3-thiazole-5-carboxamide], a new Oomycetes fungicide. In in vitro tests, ethaboxam showed inhibitory activity against isolates of Phytophthora and some Pythium spp, with MIC values ranging from 0.1 to 0.5 mg litre(-1) for nine isolates of Phytophthora infestans (Montagne) de Bary and from 1.0 to 5.0 mg litre(-1) for eight isolates of Phytophthora capsici Leonian. In tests to determine time and concentration for complete inactivation of each pathogen (five isolates of P infestans and five isolates of P capsici), ethaboxam inactivated all isolates of P infestans within 48h at 10 mg litre(-1) and those of P capsici within 96 h at 10 mg litre(-1). Ethaboxam effectively suppressed development of tomato late blight caused by P infestans and pepper Phytophthora blight caused by P capsici in the studies conducted to determine its preventive, curative, persistent and systemic activity. These results show that ethaboxam has desirable fungicidal characteristics as an Oomycetes fungicide.  相似文献   

Use of a crush-reducing device to decrease crushing of suckling piglets by sows     

Jeon JH  Kim DJ  Han JH  Yeon SC  Bahng SH  Myeong BS  Chang HH 《Journal of animal science》2005,83(6):1397-1402

Weanling pigs tend to avoid wind, and suckling piglets are thought to be more sensitive to wind than weanlings, owing to their thinner s.c. fat layer. We developed a crush-reducing device based on the anticipated behavior of suckling piglets toward wind and evaluated the performance of the device through field testing. The crush-reducing device consisted of six photo sensors, a controller, six solenoid valves, and an air compressor. In this study, 206 sows and their suckling piglets (Landrace xYorkshire) were investigated to ascertain the individual effects of several factors. Some of the newborn piglets were cross-fostered before the crush-reducing device was implemented. Litter weights were measured on d 0 and 4 to determine the influence of the crush-reducing device on the weights of suckling piglets. The crushing of suckling piglets by sows was affected by season (P < 0.01) and litter size (P < 0.05), but not by the parity of the sows; however, the number of crushed piglets per litter was less (P < 0.01) in the crush-reducing device group (0.05 +/- 0.02 crushed piglets/litter) than in the control group (0.23 +/- 0.04 crushed piglets/litter), regardless of litter size or season. The BW gain of suckling piglets did not differ between the control and the crush-reducing device groups. Based on these results, the crush-reducing device is expected to decrease the number of crushed piglets per litter without influencing the BW gain of suckling piglets, thereby greatly contributing to the productivity of pig breeders.  相似文献