Relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats     

XU Rui  ZHANG Yun  ZHANG Mei  GE Zhi-ming  CHEN Wen-qiang  LI Xiu-chang  CAI Heng  ZHU Hui 《园艺学报》2003,19(5):642-644

AIM:To elucidate the relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats (SHR).METHODS:Intracellular free calcium concentrations were measured by Fura 2 methodology and left ventricular function quantitated by cardiac catheterization in 20 SHR aged 10, 22, and 34 weeks and 20 age-matched Wistar-kyoto (WKY) rats.RESULTS:(1) The systolic blood pressure(SBP), intracellular calcium concentrations and left ventricular mass / body weight index (LVM/BW) were significantly higher in all three age groups of SHR than the corresponding groups of WKY; (2) Compared with age-matched WKY groups, the peak left ventricular pressure descending rate(-dp／dt_max) decreased while left ventricular relaxation time constant (τ)increased significantly in SHR aged 22 and 34 weeks. The peak left ventricular pressure ascending rate(dp／dt_max) and the left ventricular contractility index were significantly increased only in the 34 weeks SHR; (3) Intracellular calcium concentrations showed a positive correlation with LVM/BW,SBP,-dp／dt_max and τ(r=0.47-0.83,P<0.01)and a negative correlation with dp／dt_max and the left ventricular contractility index (r=-0.46,P<0.05 and r=-0.81, P<0.01).CONCLUSION:Intracellular calcium overload is one of the potential mechanisms in the induction of left ventricular hypertrophy as well as of systolic and diastolic dysfunction.  相似文献   

Effects of matrix metalloproteinases on ventricular remodelingfollowing myocardial ischemia in the rat     

ZHANG En-guang  ZHANG Huai-qin  LIN Jie  HUANG Wei-jian  LI Shang-gong  XU Li-xin 《园艺学报》2003,19(5):653-656

AIM:To examine the relationship between the activity of matrix metallproteinases(MMPs) and ventricular remodeling following myocardial ischemia in the rat.METHODS:The model of myocardial ischemia(MI) in the rat was established by isoprenaline(ISP). The activity of MMPs was measured by zymography and collagen concentration was assessed by the method of chloramine T, so did I/III collagen ratio by immunohistochemical staining. The microstructure of myocardium was also observed by electron microscope.RESULTS:The activity of MMP-2 in myocardial ischemia group (group M) increased by 5.8 folds at 1 st week(P＜0.01), 2.3 folds at 2 nd week(P＜0.01) and 1.7 flods at 4 th week(P＜0.05) compared with control group (group C) and MMP-9's activity in group M increased by 4.9 folds (P＜0.01), 1.9 flods(P＜0.01) and 1.4 folds(P＜0.05), respectively. Collagen amount and I/III collagen ratio in group M increased compared with that in group C at 2 nd week and 4 th week. It showed that cardiac myocytes in group M were necrosed and collagen grew abundantly in interstitium under electron microscope.CONCLUSION:The activity of MMPs in the myocardial interstitium increased following myocardial ischemia, then collagen amount and I/III collagen ratio increased, which may be the major causes of ventricular remodeling.  相似文献   

Protective effect of onychin on the human umbilical vein endothelial cells injured by menadione     

GUO Yu  ZHU Bing-yang  YAN Feng-xiang  HUANG Hong-lin  LIAO Duan-fang 《园艺学报》2003,19(1):10-13

AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO₂^-／NO₃^- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO₂^-／NO₃^- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway.  相似文献   

Detection,cloning and expression of bone morphogenetic protein-1 from human osteosarcoma cell lines     

CHEN Dong-ying  ZHU Quan-sheng  LIU Chao  QIU Ju-shi 《园艺学报》2003,19(6):751-756

AIM:To manufacture recombinant protein of the highly conserved domain in human bone morphogenetic protein-1(BMP-1) using gene engineering methods as antigen for making wide spectrum antibody to BMP-1.METHODS:We analyzed the gene sequences and protein structures of BMP-1 and its related proteins, and chose a highly conserved fragment as target gene. Total RNA was prepared from human osteosarcoma cell line Saos-2, then the target gene was amplified with RT-PCR. The PCR product was cloned into prokaryotic expression vector pMAL c2 to get recombinant vector BMP-1(322-588aa)-pMAL c2. After transforming the recombinant plasmid into DH5-alpha and screening, several prositive clones were got for sequencing. Finally the transformed cells was induced with IPTG to get fusion protein.RESULTS:The BMP-1 gene fragment was successfully cloned into vector pMAL c2, and was able to express efficiently with IPTG inducement. The amount of expressed fusion protein is about 66%-72% in total volume of bacterial proteins.CONCLUSIONS:The recombinant protein contains several key domains(2 CUB domains and 1 EGF domain), which are shared by BMP-1 and its related proteins. Specific wide spectrum antibody to human BMP-1 and its related proteins may be generated with this recombinant protein antigen.  相似文献   

Biological characteristics of long passaging rat mesenchymal stem cells     

LEI Jun-xia  LI Hao-wei  HUANG Chun-nong  ZHU Mei-ling  WEN Guan-mei  ZHANG Xiu-ming  LI Yan  LI Shu-nong 《园艺学报》2003,19(10):1325-1330

AIM:To investigate multi-potential of rat bone marrow mesenchymal stem cells (rBMMSC) and mutation inclination, the rBMMSC were long passaged in vitro. METHODS:Cellular cycles of different passages were assayed by FACSan flow cytometry and karyotypes of passage 6, passage 25 and passage 45 were compared by G-binding analysis. RESULTS:The early passages and long-term passages all showed strong proliferation; passage 6, passage 25 and passage 45 all showed normal karyotype. CONCLUSION:Long-term culture and passage of rBMMSC still remains strong proliferation. With this capability, the mutation inclination is not enhanced.  相似文献   

Effect of Yigu capsule drug-containing serum on apoptosis and tartrate resistant acid phosphatase secretion in rat osteoclasts     

SHU Xiao-chun  ZHANG Rong-hua  ZHU Xiao-feng  PENG Ke-ping 《园艺学报》2003,19(9):1234-1237

AIM:The effects of Yigu capsule on tartrate resistant acid phosphatase (TRAP) secretion and apoptosis in rat osteoclasts were investigated in order to further explore its mechanism of preventing and treating osteoporosis.METHODS:(1) Twenty-month-old Sprague-daweley rats were randomly divided into two groups(Yigu capsule group and saline group), and the drug-containing serum and control serum were prepared. (2) The newborn Sprague-daweley rat osteoclasts were cultured with different concentrations of Yigu capsule drug-containing serum. At different time point, TRAP activity was measured and the survival osteoclast was counted under reverse microscope.The percentage of osteoclast apoptosis was observed under fluorescence microscope after acridine orange staining.RESULTS:TRAP activity was lower and the percentage of osteoclast apoptosis was higher in drug-containing serum group than in control group at 24, 48 and 72 h(P<0.01), respectively, and the survival osteoclasts were less in drug-containing serum group than in control groups at 24, 48 and 72 h(P<0.01).CONCLUSIONS:These data suggest that Yigu capsule drug-containing serum induces apoptosis and inhibits TRAP activity in osteoclasts, which may be one of the mechanisms of Yigu capsule preventing and treating osteoporosis.  相似文献   

黑河干流中游地区耗水量变化的历史分析   总被引：9，自引：6，他引：9  

任建华  李万寿  张婕 《干旱区研究》2002,19(1):18-22

黑河流域水资源极其匮乏 ,加之地区上的配置不均衡和中游地区水土资源的过度开发导致了黑河下游严重生态环境问题 ,已影响到我国北方广大地区生态环境质量 ,受到国家的高度重视和全社会的广泛关注。黑河水资源开发利用主要集中在中游地区 ,该区是全国重要的商品粮、蔬菜和经济作物生产基地之一 ,耗水量占流域耗水量的 85 %以上 ,是黑河的主要耗水区和径流利用区。本文利用黑河上游控制站莺落峡站和中游控制站正义峡站水文资料 ,分析了黑河中游耗水量的变化 ,结果表明 :2 0世纪 80年代以前黑河中游耗水量在 4.2 0× 10 8m3 左右 ,比较稳定 ;进入 2 0世纪 80年代以来耗水量明显增加 ,80年代耗水量比 40年代末期至 70年代增加了 2 .0× 10 8～ 2 .5×10 8m3 左右 ;90年代耗水量又比 80年代增加了 1.6 0× 10 8m3 左右。 80年代以来流域耗水量呈明显增加的趋势 ,这是以人类活动影响为主的结果  相似文献   

敌死虫对不同发育阶段烟粉虱的生物活性测定   总被引：4，自引：1，他引：4  

吴青君  徐宝云  徐维红  张友军  朱国仁 《植物保护》2002,28(4):11-13

敌死虫对烟粉虱成虫具有较强的驱避作用 ,99.1%敌死虫200、100、50倍液药后 2h～120h对黄瓜上烟粉虱的驱避率均在90%以上 ,50倍液处理药后3d的驱避率为98%～100%。药剂处理的非洲菊上烟粉虱成虫产卵量显著低于对照。敌死虫对烟粉虱卵具有一定活性 ,致死中量为10468.6mg/kg ;对若虫活性较高 ,对1、2、3龄若虫的致死中量分别为165.81、199.46、232.38mg/kg ,稀释倍数分别为5976、4968、4264倍。  相似文献   

苏云金芽孢杆菌cry基因在大肠杆菌中表达产物和生物活性   总被引：4，自引：0，他引：4  

韩岚岚  宋福平  李长友  张杰  赵奎军  黄大昉 《植物保护》2002,28(5):5-9

研究了几种Bt cry基因于大肠杆菌(Escherichia coli)中表达产物在pH 10.0的50mmol/L碳酸钠和20mmol/L乙醇胺溶解液中的溶解性 ,发现同样的Cry蛋白在碳酸钠中的溶解度大于乙醇胺。通过胰蛋白酶消化 ,明确Cry1Ca7、Cry1Ia8酶解产物为 38kD多肽 ;Cry1Ie1、Cry1Cb2、Cry2Ab4酶解产物为 41kD多肽 ;Cry1Ac酶解产物为60kD多肽。采用FPLC层析方法对 6种原毒素及其酶解后得到的毒素多肽进行了分离纯化 ,比较了原毒素和毒素的杀虫活性的差异。其结果表明 ,Cry1Ac的原毒素和毒素对棉铃虫初孵幼虫的校正死亡率均为 100% ,Cry2Ab4的原毒素的毒力高于其酶解毒素。  相似文献   

玉米疯顶病种子传播研究   总被引：2，自引：0，他引：2  

杨建国  金晓华  谢爱婷  王晓鸣  朱振东 《中国植保导刊》2002,22(6):3-4

连续3a对不同来源的玉米种子,利用显微镜镜检和试种观察植株发病显症的方法,检测其携带玉米疯顶病病菌状况。结果表明,病株病穗籽粒、病株健穗籽粒、病田健株籽粒都有较高的带菌率。因此,病菌随种子调运远距离传播是造成各地病害突发的主要原因。  相似文献