Synthesis,insecticidal activity,and ensembled docking of nitroguanidines bearing S- and R-proline     

Alfredo Rodríguez-Hernández  Israel Bonilla-Landa  Abraham Vidal-Limon  Arturo Ibarra-Juárez  Felipe Barrera-Méndez  Francisco Javier Enríquez Medrano  Ramón E. Díaz de León  José Luis Olivares-Romero 《Pest management science》2023,79(5):1912-1921

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Detection of Ecballium elaterium in hedgerow olive orchards using a low-cost uncrewed aerial vehicle and open-source algorithms     

Jorge Torres-Sánchez  Francisco Javier Mesas-Carrascosa  Fernando Pérez-Porras  Francisca López-Granados 《Pest management science》2023,79(2):645-654

BACKGROUND

Ecballium elaterium (common name: squirting cucumber) is an emerging weed problem in hedgerow or superintensive olive groves under no tillage. It colonizes the inter-row area infesting the natural or sown cover crops, and is considered a hard-to-control weed. Research in other woody crops has shown E. elaterium has a patchy distribution, which makes this weed susceptible to design a site-specific control strategy only addressed to E. elaterium patches. Therefore, the aim of this work was to develop a methodology based on the analysis of imagery acquired with an uncrewed aerial vehicle (UAV) to detect and map E. elaterium infestations in hedgerow olive orchards.

RESULTS

The study was conducted in two superintensive olive orchards, and the images were taken using a UAV equipped with an RGB sensor. Flights were conducted on two dates: in May, when there were various weeds infesting the orchard, and in September, when E. elaterium was the only infesting weed. UAV-orthomosaics in the first scenario were classified using random forest models, and the orthomosaics from September with E. elaterium as the only weed, were analyzed using an unsupervised algorithm. In both cases, the overall accuracies were over 0.85, and the producer's accuracies for E. elaterium ranged between 0.74 and 1.00.

CONCLUSION

These results allow the design of a site-specific and efficient herbicide control protocol which would represent a step forward in sustainable weed management. The development of these algorithms in free and open-source software fosters their application in small and medium farms. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.  相似文献   

First report of Seville root-knot nematode (Meloidogyne hispanica) infecting tobacco in the Brazil     

de Ramos  Eduarda Kalena Kirsch  Pazdiora  Paulo Cesar  Dallagnol  Leandro José  de Souza  Mayara Rodrigues  de Araujo Filho  Jeronimo Vieira 《植物病害和植物保护杂志》2023,130(1):211-214

Journal of Plant Diseases and Protection - Tobacco (Nicotiana tabacum L.) is one of the major nonfood crops in the Southern of Brazil. During 2019/2020 and 2020/2021 crop seasons, Root Knot...  相似文献   

Genetic basis and origin of resistance to acetolactate synthase inhibitors in Amaranthus palmeri from Spain and Italy     

Alfredo Manicardi  Laura Scarabel  Josep María Llenes  José María Montull  María Dolores Osuna  Joel Torra Farré  Andrea Milani 《Pest management science》2023,79(12):4886-4896

BACKGROUND

Amaranthus palmeri is an aggressive annual weed native to the United States, which has become invasive in some European countries. Populations resistant to acetolactate synthase (ALS) inhibitors have been recorded in Spain and Italy, but the evolutionary origin of the resistance traits remains unknown. Bioassays were conducted to identify cross-resistance to ALS inhibitors and a haplotype-based genetic approach was used to elucidate the origin and distribution of resistance in both countries.

RESULTS

Amaranthus palmeri populations were resistant to thifensulfuron-methyl and imazamox, and the 574-Leu mutant ALS allele was found to be the main cause of resistance among them. In two Spanish populations, 376-Glu and 197-Thr mutant ALS alleles were also found. The haplotype analyses revealed the presence of two and four distinct 574-Leu mutant haplotypes in the Italian and Spanish populations, respectively. None was common to both countries, but some mutant haplotypes were shared between geographically close populations or between populations more than 100 km apart. Wide genetic diversity was found in two very close Spanish populations.

CONCLUSION

ALS-resistant A. palmeri populations were introduced to Italy and Spain from outside Europe. Populations from both countries have different evolutionary histories and originate from independent introduction events. ALS resistance then spread over short and long distances by seed dispersal. The higher number and genetic diversity among mutant haplotypes from the Spanish populations indicated recurrent invasions. The implementation of control tactics to limit seed dispersal and the establishment of A. palmeri is recommended in both countries. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.  相似文献   

Hepatitis E virus in Cuba: A cross-sectional serological and virological study in pigs and people occupationally exposed to pigs     

Maria Caridad Montalvo Villalba  Chantal J. Snoeck  Licel de los Angeles Rodriguez Lay  Aurélie Sausy  Dayesi Hernández López  Marite Bello Corredor  Barbara Marrero Sanchéz  Judith M. Hübschen 《Zoonoses and public health》2023,70(1):58-68

Surveillance of hepatitis E virus (HEV) in risk groups is an important strategy to monitor its circulation pattern and to timely detect changes thereof. The aims of this cross-sectional study were to estimate the prevalence of HEV infections in pigs and humans from different regions of the country, to identify risk factors for increasing anti-HEV IgG prevalence and to characterize HEV strains. The presence of anti-HEV antibodies was assessed by commercial ELISA in serum samples from the general population, farm and slaughterhouse employees, as well as pigs sampled in the three regions of Cuba from February to September 2016. Overall, individuals with occupational exposure to swine or swine products (70/248, 28.2%) were 4 times more likely to be seropositive compared to the general population (25/285, 8.7%; OR: 4.18; p < .001). Within the risk group, risk factors included age, number of years working in a professional activity with direct exposure to swine, geographic region and distance between residence and closest professional swine setting, while wearing gloves had a protective effect. Prevalence of total anti-HEV antibodies in swine was 88.2% (165/187) and HEV RNA was detected by real-time RT-PCR in 9.2% (16/173) swine stools. All HEV strains sequenced clustered within genotype 3. Some strains clearly belonged to subtype 3a, while another group of strains was related with subtypes 3b and 3 k but partial HEV sequences did not allow unequivocal subtype assignment. These findings suggest that the high HEV exposure in Cuban individuals with swine-related occupations could be due to enzootic HEV in certain regions, direct contact with infectious animals or their products as well as environmental contamination.  相似文献   

Pilot study of the ultrasonographic examination of the intact and transected medial glenohumeral ligament in dogs     

Francesco Gemignani  Mathieu Harel  Véronique Livet  Anthony Barthélemy  Émilie Ségard  Thibaut Cachon  Juliette Sonet 《Veterinary radiology & ultrasound》2023,64(2):306-313

Medial glenohumeral ligament injury is commonly reported during medial shoulder joint instability in dogs. Arthroscopy is considered the gold standard procedure, but it is invasive and requires distension of the joint. Ultrasonographic examination of the medial glenohumeral ligament has been studied as a possible, less invasive alternative to arthroscopy however it has not been considered a useful method of assessment due to the interference of the probe with the pectoral muscles. The aims of this prospective analytical randomized pilot study were to develop a standardized ultrasound protocol for visualizing the canine medial glenohumeral ligament and to compare goniometry and ultrasound findings in cadaver dogs with versus without transection of the medial glenohumeral ligament. Nine adult Beagle cadavers (18 shoulders) were used. The first six shoulders were used in a preliminary study to describe an ultrasound technique to identify the medial glenohumeral ligament. Arthroscopy was performed on the remaining 12 shoulders, with six randomly selected medial glenohumeral ligaments from these shoulders, transected during the procedure. Ultrasound examination was performed after each arthroscopic procedure by an ultrasonographer blinded to the patient group. Four medial glenohumeral ligaments (67%) were correctly identified during the preliminary study. Ultrasonographic examination failed to diagnose the transection of all six medial glenohumeral ligaments in the second part of the study. No difference was observed in the ligament thickness between the dogs with and without a transected medial glenohumeral ligament. Dogs with a transected medial glenohumeral ligament had a wider articular space compared to dogs without a transected ligament (P < 0.001), and an articular space wider than 8.2 mm was discriminatory of a transected medial glenohumeral ligament in all the shoulders. In conclusion, the medial glenohumeral ligament could be identified with a medial ultrasonographic approach of the shoulder and a wider articular space can be a sign of a medial shoulder joint instability. Further studies are needed to confirm these preliminary findings in living dogs, with and without shoulder instability.  相似文献   

Fertility of Holstein cows and heifers submitted to timed artificial insemination and receiving one or two doses (12 h apart) of semen     

Miguel Mellado  Lucía M. Treviño  María I. Chavez  Francisco G. Véliz  Ulises Macías-Cruz  Leonel Avendaño-Reyes  José E. García 《Reproduction in domestic animals》2023,58(1):39-47

The objective of this retrospective study was to assess the effect of receiving a single (n = 50,285) or double (n = 4392) artificial insemination (AI), 12 h apart, within a timed artificial insemination protocol on pregnancy per AI (P/AI) in nulliparous heifers (inseminated with either sex-sorted or conventional semen) and pluriparous Holstein cows in a commercial dairy herd. Also, this study aimed to investigate the relationship between temperature-humidity index (THI) and time of the first AI and fertility. Fertility of cows receiving two AI with normothermia (THI <68) was higher (p < .05) than cows receiving a single AI (42.9% vs. 36.4%). P/AI of cows receiving two AI with severe heat stress (THI >85) was higher (p < .05) than cows receiving a single AI (21.0% vs. 12.6%). Regardless of heat stress conditions, applying the first AI in the morning increased (p < .05) P/AI in cows with double AI than in cows whose first AI occurred in the afternoon (38.4 vs. 33.3%). With moderate heat stress, and sexed-sorted semen, P/AI to timed AI was higher (65.0 vs. 51.9%; p < .05) in heifers receiving double AI than those serviced once. It was concluded that double AI, 12 h apart, enhanced fertility at timed AI than herd mates with a single AI, particularly with heat stress at breeding.  相似文献   

Using midday stem water potential for scheduling deficit irrigation in mid–late maturing peach trees under Mediterranean conditions     

José Manuel Mirás-Avalos  Francisco Pérez-Sarmiento  Rosalía Alcobendas  Juan José Alarcón  Oussama Mounzer  Emilio Nicolás 《Irrigation Science》2016,34(2):161-173

Irrigation techniques that reduce water applications are increasingly applied in areas with scarce water resources. In this study, the effect of two regulated deficit irrigation (RDI) strategies on peach [Prunus persica (L.) Batsch cv. “Catherine”] performance was studied over three growing seasons. The experimental site was located in Murcia (SE Spain), a Mediterranean region. Two RDI strategies (restricting water applications at stage II of fruit development and postharvest) based on stem water potential (Ψ_s) thresholds (?1.5 and ?1.8 MPa during fruit growth and ?1.5 and ?2.0 MPa during postharvest) were compared to a fully irrigated control. Soil water content (θ_v), Ψ_s, gas exchange parameters, vegetative growth, crop load, yield and fruit quality were determined. RDI treatments showed significantly lower values of θ_v and Ψ_s than control trees when irrigation water was restricted, causing reductions in stomatal conductance and photosynthesis rates. Vegetative growth was reduced by RDI, as lower shoot lengths and pruning weights were observed under those treatments when compared to control. However, fruit size and yield were unaffected, and fruit quality was slightly improved by RDI. Water savings from 43 to 65 % were achieved depending on the year and the RDI strategy, and no negative carryover effect was detected during the study period. In conclusion, RDI strategies using Ψ_s thresholds for scheduling irrigation in mid–late maturing peach trees under Mediterranean conditions are viable options to save water without compromising yield and even improving fruit quality.  相似文献   

Irrigation scheduling in a mature peach orchard using tensiometers and dendrometers     

Shao-Hua Li  Jean-Gérard Huguet  Claude Bussi 《Irrigation and Drainage Systems》1989,3(1):1-12

Three trickle irrigation schedules, two of which were scheduled according to soil water potential ( soil) (tensiometer method) and daily stem contraction (DSC) (dendrometer method) respectively and the other one was a schedule of restricted water supply, were applied to a mature peach orchard.The annual water application based on soil was greater than that based on DSC. However, tree growth, fruit size and leaf water potential (leaf) on the trees in the dendrometer scheduling plot did not differ from those in the tensiometer scheduling plot while the premature fruit drop and fruit bud initiation were greatly different. The restricted water supply treatment limited significantly both tree and fruit growth. In addition, the lower leaf was observed on the trees in this plot.Further study shows that use of the dendrometer method for scheduling irrigation satisfies the water needs of the plant and that the tensiometer method is less accurate.Abbreviations leaf leaf water potential - soil soil water potential - DSC daily stem contraction - LVDT linear variable displacement transducer - PET potential evapotranspiration  相似文献   

Effects of irrigation regimes on the yield and water use of strawberry     

Lydia Serrano  Xavier Carbonell  Robert Savé  Oriol Marfà  Josep Peñuelas 《Irrigation Science》1992,13(1):45-48

Summary Strawberry plants (Fragaria x annanasa D. cv Chandler) were grown in field plots and in drainage lysimeters under controlled soil moisture regimes. Four irrigation treatments were established by watering the plants when soil water potential reached -0.01, -0.03,-0.05 and -0.07 MPa. The maximum yield was attained at -0.01 MPa soil water potential. Differences in yield were caused by both changes in the number of fruits per plant and in the fresh weight per fruit. Yield reductions were associated with reductions in total assimilation rate resulting from the decreased assimilatory surface area in plants irrigated at lower soil water potentials. The crop water production function calculated on a fruit fresh weight basis resulted in a yield response factor (K _y) of 1.01.  相似文献